Reviewed, UniProtKB/Swiss-Prot P12018 (VPREB_HUMAN)

Names and origin

Protein names

Recommended name:
    Immunoglobulin iota chain
Alternative name(s):
    V(pre)B protein
      Short name=VpreB protein
    CD179 antigen-like family member A
    CD_antigen=CD179a

Gene names

Name:	VPREB1
Synonyms:	VPREB

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	145 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Associates with the Ig-mu chain to form a molecular complex that is expressed on the surface of pre-B-cells. This complex presumably regulates ig gene rearrangements in the early steps of B-cell differentiation.
Subunit structure	Associates non-covalently with IGLL1.
Tissue specificity	Only expressed by pre-B-cells.
Sequence similarities	Belongs to the immunoglobulin superfamily.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Signal
PTM	Disulfide bond
Technical term	3D-structure
Gene Ontology (GO)
Biological process	immune response Non-traceable author statement. Source: UniProtKB
Cellular component	extracellular region Non-traceable author statement. Source: UniProtKB
Molecular function	antigen binding Non-traceable author statement. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ABL1	P00519	1	EBI-1753454,EBI-375543

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Potential

Chain

20 – 145

126

Immunoglobulin iota chain

PRO_0000015000

Regions

Region

20 – 41

22

Framework-1

Region

42 – 56

15

Complementarity-determining-1

Region

57 – 70

14

Framework-2

Region

71 – 81

11

Complementarity-determining-2

Region

82 – 115

34

Framework-3

Amino acid modifications

Disulfide bond

41 ↔ 115

By similarity

Natural variations

Natural variant

76

1

D → N: dbSNP rs1320.

VAR_024503

Natural variant

122

1

S → L: dbSNP rs11089979.

VAR_029133

Natural variant

132

1

E → K: dbSNP rs5995720.

VAR_029134

Experimental info

Sequence conflict

10

1

L → H in AAA61292. Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

145

Helix Strand Turn

Details...

Beta strand

27 – 32

6

Beta strand

37 – 43

7

Beta strand

45 – 47

3

Helix

49 – 51

3

Beta strand

53 – 59

7

Beta strand

66 – 73

8

Beta strand

76 – 79

4

Beta strand

87 – 92

6

Turn

93 – 96

4

Beta strand

97 – 102

6

Helix

107 – 109

3

Beta strand

111 – 118

8

Sequences

	Sequence		Length	Mass (Da)
	P12018-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 197665B13AF64D46	FASTA	145	16,605
	10 20 30 40 50 60 MSWAPVLLML FVYCTGCGPQ PVLHQPPAMS SALGTTIRLT CTLRNDHDIG VYSVYWYQQR 70 80 90 100 110 120 PGHPPRFLLR YFSQSDKSQG PQVPPRFSGS KDVARNRGYL SISELQPEDE AMYYCAMGAR 130 140 SSEKEERERE WEEEMEPTAA RTRVP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human pre-B cell receptor: structural constraints for a tentative model of the pseudo-light (psi L) chain." Guelpa-Fonlupt V., Bossy D., Alzari P., Fumoux F., Fougereau M., Schiff C. Mol. Immunol. 31:1099-1108(1994) [PubMed: 7935499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"One-megabase sequence analysis of the human immunoglobulin lambda gene locus." Kawasaki K., Minoshima S., Nakato E., Shibuya K., Shintani A., Schmeits J.L., Wang J., Shimizu N. Genome Res. 7:250-261(1997) [PubMed: 9074928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004) [PubMed: 15461802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Structure and pre-B lymphocyte restricted expression of the VpreB in humans and conservation of its structure in other mammalian species." Bauer S.R., Kudo A., Melchers F. EMBO J. 7:111-116(1988) [PubMed: 3258819] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-139.
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

S74019 Genomic DNA. Translation: AAB32118.1.
D86992 Genomic DNA. Translation: BAA19987.1.
D88270 Genomic DNA. Translation: BAA20030.1.
CR456609 mRNA. Translation: CAG30495.1.
M34927 Genomic DNA. Translation: AAA61292.1.

IPI

IPI00021712.

PIR

I57832.
S00258.

RefSeq

NP_009059.1.

UniGene

Hs.247979

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2H32	X-ray	2.70	A	20-145	[»]
2H3N	X-ray	2.30	A/C	21-119	[»]
3BJ9	X-ray	2.00	1	22-121	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P12018. 1 interaction.

Proteomic databases

PRIDE

P12018.

Genome annotation databases

Ensembl

ENSG00000169575. Homo sapiens. [Contig view]

GeneID

7441.

KEGG

hsa:7441.

Organism-specific databases

GeneCards

GC22P020923.

H-InvDB

HIX0016286.

HGNC

HGNC:12709. VPREB1.

MIM

605141. gene.

PharmGKB

PA37324.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P12018.

HOVERGEN

P12018.

OMA

P12018. LHQPPAM.

Gene expression databases

ArrayExpress

P12018.

Bgee

P12018.

CleanEx

HS_VPREB1.

GermOnline

ENSG00000169575. Homo sapiens.

Family and domain databases

InterPro

IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR013106. Ig_V-set.
IPR003596. Ig_V-set_sub.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

Pfam

PF07686. V-set. 1 hit.
[Graphical view]

SMART

SM00406. IGv. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

29136.

SOURCE

Search...

Entry information

Entry name

VPREB_HUMAN

Accession

Primary (citable) accession number: P12018

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)