P12018 (VPREB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Immunoglobulin iota chain

Alternative name(s):
CD179 antigen-like family member A
Protein VPreB1
V(pre)B protein
Short name=VpreB protein
CD_antigen=CD179a

Gene names

Name:	VPREB1
Synonyms:	VPREB

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	145 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Associates with the Ig-mu chain to form a molecular complex that is expressed on the surface of pre-B-cells. This complex presumably regulates Ig gene rearrangements in the early steps of B-cell differentiation.
Subunit structure	Associates non-covalently with IGLL1. Ref.8
Tissue specificity	Only expressed by pre-B-cells.
Sequence similarities	Belongs to the immunoglobulin superfamily. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Signal
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	immune response Non-traceable author statement. Source: UniProtKB
Cellular_component	extracellular region Non-traceable author statement. Source: UniProtKB
Molecular_function	antigen binding Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Potential

Chain

20 – 145

126

Immunoglobulin iota chain

PRO_0000015000

Regions

Domain

20 – 132

113

Ig-like V-type

Region

20 – 41

22

Framework-1

Region

42 – 56

15

Complementarity-determining-1

Region

57 – 70

14

Framework-2

Region

71 – 81

11

Complementarity-determining-2

Region

82 – 115

34

Framework-3

Amino acid modifications

Disulfide bond

41 ↔ 115

Ref.8 Ref.9

Natural variations

Natural variant

76

1

D → N.
Corresponds to variant rs1320 [ dbSNP | Ensembl ].

VAR_024503

Natural variant

122

1

S → L.
Corresponds to variant rs11089979 [ dbSNP | Ensembl ].

VAR_029133

Natural variant

132

1

E → K.
Corresponds to variant rs5995720 [ dbSNP | Ensembl ].

VAR_029134

Experimental info

Sequence conflict

10

1

L → H in AAA61292. Ref.6

Secondary structure

1

.

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145

Helix Strand Turn

Details...

Beta strand

22 – 24

3

Beta strand

27 – 32

6

Beta strand

37 – 43

7

Beta strand

45 – 47

3

Helix

49 – 51

3

Beta strand

54 – 59

6

Beta strand

66 – 73

8

Beta strand

76 – 79

4

Beta strand

87 – 92

6

Helix

93 – 95

3

Beta strand

97 – 102

6

Helix

107 – 109

3

Beta strand

111 – 117

7

Sequences

	Sequence		Length	Mass (Da)
	P12018 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 197665B13AF64D46	FASTA	145	16,605
	10 20 30 40 50 60 MSWAPVLLML FVYCTGCGPQ PVLHQPPAMS SALGTTIRLT CTLRNDHDIG VYSVYWYQQR 70 80 90 100 110 120 PGHPPRFLLR YFSQSDKSQG PQVPPRFSGS KDVARNRGYL SISELQPEDE AMYYCAMGAR 130 140 SSEKEERERE WEEEMEPTAA RTRVP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human pre-B cell receptor: structural constraints for a tentative model of the pseudo-light (psi L) chain." Guelpa-Fonlupt V., Bossy D., Alzari P., Fumoux F., Fougereau M., Schiff C. Mol. Immunol. 31:1099-1108(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"One-megabase sequence analysis of the human immunoglobulin lambda gene locus." Kawasaki K., Minoshima S., Nakato E., Shibuya K., Shintani A., Schmeits J.L., Wang J., Shimizu N. Genome Res. 7:250-261(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H. Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"Structure and pre-B lymphocyte restricted expression of the VpreB in humans and conservation of its structure in other mammalian species." Bauer S.R., Kudo A., Melchers F. EMBO J. 7:111-116(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-139.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"Structural insight into pre-B cell receptor function." Bankovich A.J., Raunser S., Juo Z.S., Walz T., Davis M.M., Garcia K.C. Science 316:291-294(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 21-119 IN COMPLEX WITH IGLL1, SUBUNIT, DISULFIDE BOND.
[9]	"Engineering and characterization of a single chain surrogate light chain variable domain." Morstadt L., Bohm A., Yuksel D., Kumar K., Stollar B.D., Baleja J.D. Protein Sci. 17:458-465(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 95-108, DISULFIDE BOND.
+	Additional computationally mapped references.

Web resources

Wikipedia

VPREB1

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

S74019 Genomic DNA. Translation: AAB32118.1.
D86992 Genomic DNA. Translation: BAA19987.1.
D88270 Genomic DNA. Translation: BAA20030.1.
CR456609 mRNA. Translation: CAG30495.1.
CH471095 Genomic DNA. Translation: EAW59498.1.
M34927 Genomic DNA. Translation: AAA61292.1.

IPI

IPI00021712.

PIR

I57832.
S00258.

RefSeq

NP_009059.1. NM_007128.2.

UniGene

Hs.247979.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2H32	X-ray	2.70	A	20-145	[»]
2H3N	X-ray	2.30	A/C	21-119	[»]
3BJ9	X-ray	2.00	1	22-121	[»]

ProteinModelPortal

P12018.

ModBase

Search...

Protein-protein interaction databases

IntAct

P12018. 1 interaction.

MINT

MINT-7241497.

STRING

9606.ENSP00000385361.

PTM databases

PhosphoSite

P12018.

Polymorphism databases

DMDM

9911096.

Proteomic databases

PaxDb

P12018.

PRIDE

P12018.

Protocols and materials databases

DNASU

7441.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000403807; ENSP00000385361; ENSG00000169575.

GeneID

7441.

KEGG

hsa:7441.

UCSC

uc002zvx.1. human.

Organism-specific databases

CTD

7441.

GeneCards

GC22P022600.

HGNC

HGNC:12709. VPREB1.

HPA

HPA055886.

MIM

605141. gene.

neXtProt

NX_P12018.

PharmGKB

PA37324.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG47424.

HOGENOM

HOG000059537.

HOVERGEN

HBG018013.

InParanoid

P12018.

KO

K06553.

OMA

VYSIYWY.

OrthoDB

EOG4D26RB.

PhylomeDB

P12018.

Gene expression databases

ArrayExpress

P12018.

Bgee

P12018.

CleanEx

HS_VPREB1.

Genevestigator

P12018.

GermOnline

ENSG00000169575. Homo sapiens.

Family and domain databases

Gene3D

2.60.40.10. 1 hit.

InterPro

IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013106. Ig_V-set.
IPR003596. Ig_V-set_subgr.
[Graphical view]

Pfam

PF07686. V-set. 1 hit.
[Graphical view]

SMART

SM00406. IGv. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P12018.

GenomeRNAi

7441.

NextBio

29136.

SOURCE

Search...

Entry information

Entry name

VPREB_HUMAN

Accession

Primary (citable) accession number: P12018
Secondary accession number(s): B5MCG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 129 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.