P12008 (AROC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chorismate synthase
EC=4.2.3.5

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate phospholyase

Gene names

Name:	aroC
Ordered Locus Names:	b2329, JW2326

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate. HAMAP MF_00300
Cofactor	Reduced flavin.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7. HAMAP MF_00300
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the chorismate synthase family.
Sequence caution	The sequence AAA23488.1 differs from that shown. Reason: Frameshift at position 335.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chorismate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed HAMAP MF_00300
	Chain	2 – 361	360	Chorismate synthase HAMAP MF_00300		PRO_0000140584

Sequences

	Sequence		Length	Mass (Da)
	P12008 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 47C85F91B87DF093	FASTA	361	39,137
	10 20 30 40 50 60 MAGNTIGQLF RVTTFGESHG LALGCIVDGV PPGIPLTEAD LQHDLDRRRP GTSRYTTQRR 70 80 90 100 110 120 EPDQVKILSG VFEGVTTGTS IGLLIENTDQ RSQDYSAIKD VFRPGHADYT YEQKYGLRDY 130 140 150 160 170 180 RGGGRSSARE TAMRVAAGAI AKKYLAEKFG IEIRGCLTQM GDIPLDIKDW SQVEQNPFFC 190 200 210 220 230 240 PDPDKIDALD ELMRALKKEG DSIGAKVTVV ASGVPAGLGE PVFDRLDADI AHALMSINAV 250 260 270 280 290 300 KGVEIGDGFD VVALRGSQNR DEITKDGFQS NHAGGILGGI SSGQQIIAHM ALKPTSSITV 310 320 330 340 350 360 PGRTINRFGE EVEMITKGRH DPCVGIRAVP IAEAMLAIVL MDHLLRQRAQ NADVKTDIPR W

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, characterization and nucleotide sequences of the aroC genes encoding chorismate synthase from Salmonella typhi and Escherichia coli." Charles I.G., Lamb H.K., Pickard D., Dougan G., Hawkins A.R. J. Gen. Microbiol. 136:353-358(1990) [PubMed: 2182772] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The overexpression, purification and complete amino acid sequence of chorismate synthase from Escherichia coli K12 and its comparison with the enzyme from Neurospora crassa." White P.J., Millar G., Coggins J.R. Biochem. J. 251:313-322(1988) [PubMed: 2969724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: K12.
[3]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M27714 Genomic DNA. Translation: AAA23487.1. Y00720 Genomic DNA. Translation: CAA68707.1. Frameshift. M33021 Genomic DNA. Translation: AAA23488.1. Frameshift. U00096 Genomic DNA. Translation: AAC75389.1. AP009048 Genomic DNA. Translation: BAA16185.1.
PIR	SYECKR. G65005.
RefSeq	NP_416832.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P12008.
SMR	P12008. Positions 4-353.
ModBase	Search...
Protein-protein interaction databases
IntAct	P12008. 5 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000735; EBESCP00000000735; EBESCG00000000612. EBESCT00000016173; EBESCP00000015464; EBESCG00000015233.
GeneID	946814.
GenomeReviews	Gene locus JW2326 in contig AP009048_GR. Gene locus b2329 in contig U00096_GR.
KEGG	ecj:JW2326. eco:b2329.
PATRIC	32120031. VBIEscCol129921_2425.
Organism-specific databases
EchoBASE	EB0073.
EcoGene	EG10075. aroC.
Phylogenomic databases
eggNOG	COG0082.
GeneTree	EBGT00050000011090.
HOGENOM	HBG292336.
OMA	SRFTTQR.
PhylomeDB	P12008.
ProtClustDB	PRK05382.
Enzyme and pathway databases
BioCyc	EcoCyc:AROC-MONOMER. MetaCyc:AROC-MONOMER.
Gene expression databases
Genevestigator	P12008.
Family and domain databases
HAMAP	MF_00300. Chorismate_synth. [Tree]
InterPro	IPR000453. Chorismate_synth. IPR020541. Chorismate_synthase_CS. [Graphical view]
KO	K01736.
PANTHER	PTHR21085. Chorismate_synth. 1 hit.
Pfam	PF01264. Chorismate_synt. 1 hit. [Graphical view]
PIRSF	PIRSF001456. Chorismate_synth. 1 hit.
SUPFAM	SSF103263. Chorismate_synth. 1 hit.
TIGRFAMs	TIGR00033. AroC. 1 hit.
PROSITE	PS00787. CHORISMATE_SYNTHASE_1. 1 hit. PS00788. CHORISMATE_SYNTHASE_2. 1 hit. PS00789. CHORISMATE_SYNTHASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROC_ECOLI

Accession

Primary (citable) accession number: P12008
Secondary accession number(s): P78193

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 106 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program