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Protein

DNA ligase 1

Gene

cdc17

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.By similarity

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.PROSITE-ProRule annotation

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei414ATPBy similarity1
Active sitei416N6-AMP-lysine intermediatePROSITE-ProRule annotation1
Binding sitei421ATPBy similarity1
Binding sitei437ATPBy similarity1
Metal bindingi469Magnesium 1By similarity1
Metal bindingi568Magnesium 2By similarity1
Binding sitei573ATPBy similarity1
Binding sitei587ATPBy similarity1
Binding sitei593ATPBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: InterPro
  • DNA ligase (ATP) activity Source: PomBase
  • DNA ligase activity Source: PomBase
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • base-excision repair Source: PomBase
  • cell division Source: UniProtKB-KW
  • DNA biosynthetic process Source: InterPro
  • DNA ligation Source: PomBase
  • DNA recombination Source: PomBase
  • DNA repair Source: PomBase
  • nucleotide-excision repair Source: PomBase
  • Okazaki fragment processing involved in mitotic DNA replication Source: PomBase

Keywordsi

Molecular functionLigase
Biological processCell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-174414 Processive synthesis on the C-strand of the telomere
R-SPO-5358565 Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha)
R-SPO-5358606 Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta)
R-SPO-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-SPO-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SPO-69183 Processive synthesis on the lagging strand

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 1 (EC:6.5.1.1PROSITE-ProRule annotation)
Alternative name(s):
DNA ligase I
Polydeoxyribonucleotide synthase [ATP] 1
Gene namesi
Name:cdc17
ORF Names:SPAC20G8.01, SPAC57A10.13c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC20G8.01
PomBaseiSPAC20G8.01 cdc17

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000595851 – 768DNA ligase 1Add BLAST768

Proteomic databases

MaxQBiP12000
PaxDbiP12000
PRIDEiP12000

PTM databases

iPTMnetiP12000

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei165Interaction with target DNABy similarity1
Sitei438Interaction with target DNABy similarity1
Sitei619Interaction with target DNABy similarity1
Sitei644Interaction with target DNABy similarity1

Protein-protein interaction databases

BioGridi278340, 14 interactors
STRINGi4896.SPAC20G8.01.1

Structurei

3D structure databases

ProteinModelPortaliP12000
SMRiP12000
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni309 – 318Interaction with target DNABy similarity10
Regioni490 – 492Interaction with target DNABy similarity3

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated

Phylogenomic databases

HOGENOMiHOG000036006
InParanoidiP12000
KOiK10747
OMAiWIKYKRD
OrthoDBiEOG092C0FHN
PhylomeDBiP12000

Family and domain databases

Gene3Di1.10.3260.10, 1 hit
InterProiView protein in InterPro
IPR000977 DNA_ligase_ATP-dep
IPR012309 DNA_ligase_ATP-dep_C
IPR012310 DNA_ligase_ATP-dep_cent
IPR016059 DNA_ligase_ATP-dep_CS
IPR012308 DNA_ligase_ATP-dep_N
IPR036599 DNA_ligase_N_sf
IPR012340 NA-bd_OB-fold
PfamiView protein in Pfam
PF04679 DNA_ligase_A_C, 1 hit
PF01068 DNA_ligase_A_M, 1 hit
PF04675 DNA_ligase_A_N, 1 hit
SUPFAMiSSF117018 SSF117018, 1 hit
SSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00574 dnl1, 1 hit
PROSITEiView protein in PROSITE
PS00697 DNA_LIGASE_A1, 1 hit
PS00333 DNA_LIGASE_A2, 1 hit
PS50160 DNA_LIGASE_A3, 1 hit

Sequencei

Sequence statusi: Complete.

P12000-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTVFSQIPR FKQVNQYIRM STRQSDISNF FISSASHKSE HVEVSQSSSD
60 70 80 90 100
SKNVDGRSTS EKRKVESVKL VDESKHNNHD DTGTQNVERE NNIVSEAKKQ
110 120 130 140 150
KTLGSSSSSS DAVSSNNDSG ASTPIPLPIK EPPLESNARN DKLKGHATFA
160 170 180 190 200
EMVKAFTKIE NTSKRLEIID IMGTYFFGIL RDHPSDLLAC VYLSINKLGP
210 220 230 240 250
DYSGLELGIG ESIIMKAIGE STGQTLQQIK LSFHKVGDLG LVAQTSRQNQ
260 270 280 290 300
PTMFKPAALT IPFLFDSLKK IAQMSGNQSQ NRKIGVIKRL LSSCEGAEPK
310 320 330 340 350
YLIRALEGKL RLQLAEKTIL VALANATAQY HADKNGEKLS QQDRIEGEQI
360 370 380 390 400
LRDVYCQLPS YDLIVPHLIE HGLGTLRETC KLTPGIPTKP MLAKPTKQIS
410 420 430 440 450
EVLNTFDQAA FTCEYKYDGE RAQVHFTEDG KFYVFSRNSE NMSVRYPDIS
460 470 480 490 500
VSVSKWKKPD ARSFILDCEA VGWDRDENKI LPFQKLATRK RKDVKIGDIK
510 520 530 540 550
VRACLFAFDI LYLNGQPLLE TPLNERRKLL YSMFQPSTGD FTFAKHSDQK
560 570 580 590 600
SIESIEEFLE ESVKDSCEGL MVKMLEGPDS HYEPSKRSRH WLKVKKDYLS
610 620 630 640 650
GVGDSLDLIV IGAYYGKGKR TSVYGAFLLG CYDPDTETVQ SICKLGTGFS
660 670 680 690 700
EEHLETFYNQ LKDIVISKKK DFYAHSDVPA HQPDVWFEPK YLWEVLAADL
710 720 730 740 750
SLSPVYKAAI GYVQEDKGIS LRFPRFIRIR EDKSWEDATT SEQVSEFYRS
760
QVAYSQKEKE GSPAAEDY
Length:768
Mass (Da):86,581
Last modified:October 1, 1989 - v1
Checksum:i6783FF3DDC675F31
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05107 Genomic DNA Translation: CAA28754.1
CU329670 Genomic DNA Translation: CAB08176.1
PIRiA29066
RefSeqiNP_593318.2, NM_001018749.2

Genome annotation databases

EnsemblFungiiSPAC20G8.01.1; SPAC20G8.01.1:pep; SPAC20G8.01
GeneIDi2541849
KEGGispo:SPAC20G8.01

Similar proteinsi

Entry informationi

Entry nameiDNLI1_SCHPO
AccessioniPrimary (citable) accession number: P12000
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: March 28, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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