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P11988 (BGLB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-phospho-beta-glucosidase BglB

EC=3.2.1.86
Alternative name(s):
Phospho-beta-glucosidase B
Gene names
Name:bglB
Ordered Locus Names:b3721, JW3699
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length470 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the hydrolysis of phosphorylated beta-glucosides into glucose-6-phosphate (G-6-P) and aglycone. It has a high affinity for phosphorylated aromatic beta-glucosides (p-nitrophenyl-beta-glucoside, phenyl beta-glucoside, arbutin and phosphorylated salicin), and a low affinity for phosphorylated beta-methyl-glucoside. Ref.6

Catalytic activity

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Miscellaneous

Part of the cryptic bglGFBH operon.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4704706-phospho-beta-glucosidase BglB
PRO_0000063898

Sites

Active site1721Proton donor Potential
Active site3611Nucleophile By similarity

Experimental info

Sequence conflict1471D → G in AAA23511. Ref.1
Sequence conflict449 – 4513GWY → RMVC in AAA23511. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P11988 [UniParc].

Last modified July 1, 1993. Version 2.
Checksum: 2B8422B20D7E5C98

FASTA47053,161
        10         20         30         40         50         60 
MKAFPETFLW GGATAANQVE GAWQEDGKGI STSDLQPHGV MGKMEPRILG KENIKDVAID 

        70         80         90        100        110        120 
FYHRYPEDIA LFAEMGFTCL RISIAWARIF PQGDEVEPNE AGLAFYDRLF DEMAQAGIKP 

       130        140        150        160        170        180 
LVTLSHYEMP YGLVKNYGGW ANRAVIDHFE HYARTVFTRY QHKVALWLTF NEINMSLHAP 

       190        200        210        220        230        240 
FTGVGLAEES GEAEVYQAIH HQLVASARAV KACHSLLPEA KIGNMLLGGL VYPLTCQPQD 

       250        260        270        280        290        300 
MLQAMEENRR WMFFGDVQAR GQYPGYMQRF FRDHNITIEM TESDAEDLKH TVDFISFSYY 

       310        320        330        340        350        360 
MTGCVSHDES INKNAQGNIL NMIPNPHLKS SEWGWQIDPV GLRVLLNTLW DRYQKPLFIV 

       370        380        390        400        410        420 
ENGLGAKDSV EADGSIQDDY RIAYLNDHLV QVNEAIADGV DIMGYTSWGP IDLVSASHSQ 

       430        440        450        460        470 
MSKRYGFIYV DRDDNGEGSL TRTRKKSFGW YAEVIKTRGL SLKKITIKAP 

« Hide

References

« Hide 'large scale' references
[1]"Beta-glucoside (bgl) operon of Escherichia coli K-12: nucleotide sequence, genetic organization, and possible evolutionary relationship to regulatory components of two Bacillus subtilis genes."
Schnetz K., Toloczyki C., Rak B.
J. Bacteriol. 169:2579-2590(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CRYPTIC OPERON.
Strain: K12.
[2]"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Nucleotide sequence of bglC, the gene specifying enzymeIIbgl of the PEP:sugar phosphotransferase system in Escherichia coli K12, and overexpression of the gene product."
Bramley H.F., Kornberg H.L.
J. Gen. Microbiol. 133:563-573(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19.
Strain: K12.
[6]"Genetic determination of the constitutive biosynthesis of phospho--glucosidase A in Escherichia coli K-12."
Prasad I., Young B., Schaefler S.
J. Bacteriol. 114:909-915(1973) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A GLUCOSIDASE, SUBSTRATE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M16487 Genomic DNA. Translation: AAA23511.1.
L10328 Genomic DNA. Translation: AAA62072.1.
U00096 Genomic DNA. Translation: AAC76744.1.
AP009048 Genomic DNA. Translation: BAE77567.1.
M15746 Genomic DNA. Translation: AAA83838.1.
PIRB65175.
RefSeqNP_418177.1. NC_000913.3.
YP_491708.1. NC_007779.1.

3D structure databases

ProteinModelPortalP11988.
SMRP11988. Positions 4-462.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-9214N.
IntActP11988. 3 interactions.
STRING511145.b3721.

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76744; AAC76744; b3721.
BAE77567; BAE77567; BAE77567.
GeneID12932201.
948234.
KEGGecj:Y75_p3447.
eco:b3721.
PATRIC32122937. VBIEscCol129921_3844.

Organism-specific databases

EchoBASEEB0112.
EcoGeneEG10114. bglB.

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088631.
KOK01223.
OMAEINISLH.
OrthoDBEOG6F81PM.
PhylomeDBP11988.
ProtClustDBCLSK891820.

Enzyme and pathway databases

BioCycEcoCyc:EG10114-MONOMER.
ECOL316407:JW3699-MONOMER.
MetaCyc:EG10114-MONOMER.
SABIO-RKP11988.

Gene expression databases

GenevestigatorP11988.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP11988.

Entry information

Entry nameBGLB_ECOLI
AccessionPrimary (citable) accession number: P11988
Secondary accession number(s): P78124, Q2M839
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 1, 1993
Last modified: April 16, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene