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P11988

- BGLB_ECOLI

UniProt

P11988 - BGLB_ECOLI

Protein

6-phospho-beta-glucosidase BglB

Gene

bglB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolysis of phosphorylated beta-glucosides into glucose-6-phosphate (G-6-P) and aglycone. It has a high affinity for phosphorylated aromatic beta-glucosides (p-nitrophenyl-beta-glucoside, phenyl beta-glucoside, arbutin and phosphorylated salicin), and a low affinity for phosphorylated beta-methyl-glucoside.1 Publication

    Catalytic activityi

    6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei172 – 1721Proton donorSequence Analysis
    Active sitei361 – 3611NucleophilePROSITE-ProRule annotation

    GO - Molecular functioni

    1. 6-phospho-beta-glucosidase activity Source: UniProtKB
    2. hydrolase activity, hydrolyzing O-glycosyl compounds Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10114-MONOMER.
    ECOL316407:JW3699-MONOMER.
    MetaCyc:EG10114-MONOMER.
    SABIO-RKP11988.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    6-phospho-beta-glucosidase BglB (EC:3.2.1.86)
    Alternative name(s):
    Phospho-beta-glucosidase B
    Gene namesi
    Name:bglB
    Ordered Locus Names:b3721, JW3699
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10114. bglB.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4704706-phospho-beta-glucosidase BglBPRO_0000063898Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP11988.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-9214N.
    IntActiP11988. 3 interactions.
    STRINGi511145.b3721.

    Structurei

    3D structure databases

    ProteinModelPortaliP11988.
    SMRiP11988. Positions 4-462.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088631.
    KOiK01223.
    OMAiDMERIHR.
    OrthoDBiEOG6F81PM.
    PhylomeDBiP11988.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P11988-1 [UniParc]FASTAAdd to Basket

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    MKAFPETFLW GGATAANQVE GAWQEDGKGI STSDLQPHGV MGKMEPRILG    50
    KENIKDVAID FYHRYPEDIA LFAEMGFTCL RISIAWARIF PQGDEVEPNE 100
    AGLAFYDRLF DEMAQAGIKP LVTLSHYEMP YGLVKNYGGW ANRAVIDHFE 150
    HYARTVFTRY QHKVALWLTF NEINMSLHAP FTGVGLAEES GEAEVYQAIH 200
    HQLVASARAV KACHSLLPEA KIGNMLLGGL VYPLTCQPQD MLQAMEENRR 250
    WMFFGDVQAR GQYPGYMQRF FRDHNITIEM TESDAEDLKH TVDFISFSYY 300
    MTGCVSHDES INKNAQGNIL NMIPNPHLKS SEWGWQIDPV GLRVLLNTLW 350
    DRYQKPLFIV ENGLGAKDSV EADGSIQDDY RIAYLNDHLV QVNEAIADGV 400
    DIMGYTSWGP IDLVSASHSQ MSKRYGFIYV DRDDNGEGSL TRTRKKSFGW 450
    YAEVIKTRGL SLKKITIKAP 470
    Length:470
    Mass (Da):53,161
    Last modified:July 1, 1993 - v2
    Checksum:i2B8422B20D7E5C98
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti147 – 1471D → G in AAA23511. (PubMed:3034860)Curated
    Sequence conflicti449 – 4513GWY → RMVC in AAA23511. (PubMed:3034860)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16487 Genomic DNA. Translation: AAA23511.1.
    L10328 Genomic DNA. Translation: AAA62072.1.
    U00096 Genomic DNA. Translation: AAC76744.1.
    AP009048 Genomic DNA. Translation: BAE77567.1.
    M15746 Genomic DNA. Translation: AAA83838.1.
    PIRiB65175.
    RefSeqiNP_418177.1. NC_000913.3.
    YP_491708.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76744; AAC76744; b3721.
    BAE77567; BAE77567; BAE77567.
    GeneIDi12932201.
    948234.
    KEGGiecj:Y75_p3447.
    eco:b3721.
    PATRICi32122937. VBIEscCol129921_3844.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16487 Genomic DNA. Translation: AAA23511.1 .
    L10328 Genomic DNA. Translation: AAA62072.1 .
    U00096 Genomic DNA. Translation: AAC76744.1 .
    AP009048 Genomic DNA. Translation: BAE77567.1 .
    M15746 Genomic DNA. Translation: AAA83838.1 .
    PIRi B65175.
    RefSeqi NP_418177.1. NC_000913.3.
    YP_491708.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P11988.
    SMRi P11988. Positions 4-462.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9214N.
    IntActi P11988. 3 interactions.
    STRINGi 511145.b3721.

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76744 ; AAC76744 ; b3721 .
    BAE77567 ; BAE77567 ; BAE77567 .
    GeneIDi 12932201.
    948234.
    KEGGi ecj:Y75_p3447.
    eco:b3721.
    PATRICi 32122937. VBIEscCol129921_3844.

    Organism-specific databases

    EchoBASEi EB0112.
    EcoGenei EG10114. bglB.

    Phylogenomic databases

    eggNOGi COG2723.
    HOGENOMi HOG000088631.
    KOi K01223.
    OMAi DMERIHR.
    OrthoDBi EOG6F81PM.
    PhylomeDBi P11988.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10114-MONOMER.
    ECOL316407:JW3699-MONOMER.
    MetaCyc:EG10114-MONOMER.
    SABIO-RK P11988.

    Miscellaneous databases

    PROi P11988.

    Gene expression databases

    Genevestigatori P11988.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Beta-glucoside (bgl) operon of Escherichia coli K-12: nucleotide sequence, genetic organization, and possible evolutionary relationship to regulatory components of two Bacillus subtilis genes."
      Schnetz K., Toloczyki C., Rak B.
      J. Bacteriol. 169:2579-2590(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CRYPTIC OPERON.
      Strain: K12.
    2. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
      Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
      Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Nucleotide sequence of bglC, the gene specifying enzymeIIbgl of the PEP:sugar phosphotransferase system in Escherichia coli K12, and overexpression of the gene product."
      Bramley H.F., Kornberg H.L.
      J. Gen. Microbiol. 133:563-573(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19.
      Strain: K12.
    6. "Genetic determination of the constitutive biosynthesis of phospho--glucosidase A in Escherichia coli K-12."
      Prasad I., Young B., Schaefler S.
      J. Bacteriol. 114:909-915(1973) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A GLUCOSIDASE, SUBSTRATE SPECIFICITY.

    Entry informationi

    Entry nameiBGLB_ECOLI
    AccessioniPrimary (citable) accession number: P11988
    Secondary accession number(s): P78124, Q2M839
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Part of the cryptic bglGFBH operon.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3