Reviewed, UniProtKB/Swiss-Prot P11988 (BGLB_ECOLI)

Names and origin

Protein names

Recommended name:
    6-phospho-beta-glucosidase bglB
    EC=3.2.1.86

Gene names

Name:	bglB
Ordered Locus Names:	b3721, JW3699

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can hydrolyze salicin and arbutin.
Catalytic activity	6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H(2)O = D-glucose + D-glucose 6-phosphate.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	6-phospho-beta-glucosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	6-phospho-beta-glucosidase bglB		PRO_0000063898
Sites
	Active site	172	1	Proton donor Potential
	Active site	361	1	Nucleophile By similarity
Experimental info
	Sequence conflict	147	1	D → G in AAA23511. Ref.1
	Sequence conflict	449 – 451	3	GWY → RMVC in AAA23511. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P11988-1 [UniParc]. Last modified July 1, 1993. Version 2. Checksum: 2B8422B20D7E5C98	FASTA	470	53,161
	10 20 30 40 50 60 MKAFPETFLW GGATAANQVE GAWQEDGKGI STSDLQPHGV MGKMEPRILG KENIKDVAID 70 80 90 100 110 120 FYHRYPEDIA LFAEMGFTCL RISIAWARIF PQGDEVEPNE AGLAFYDRLF DEMAQAGIKP 130 140 150 160 170 180 LVTLSHYEMP YGLVKNYGGW ANRAVIDHFE HYARTVFTRY QHKVALWLTF NEINMSLHAP 190 200 210 220 230 240 FTGVGLAEES GEAEVYQAIH HQLVASARAV KACHSLLPEA KIGNMLLGGL VYPLTCQPQD 250 260 270 280 290 300 MLQAMEENRR WMFFGDVQAR GQYPGYMQRF FRDHNITIEM TESDAEDLKH TVDFISFSYY 310 320 330 340 350 360 MTGCVSHDES INKNAQGNIL NMIPNPHLKS SEWGWQIDPV GLRVLLNTLW DRYQKPLFIV 370 380 390 400 410 420 ENGLGAKDSV EADGSIQDDY RIAYLNDHLV QVNEAIADGV DIMGYTSWGP IDLVSASHSQ 430 440 450 460 470 MSKRYGFIYV DRDDNGEGSL TRTRKKSFGW YAEVIKTRGL SLKKITIKAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Beta-glucoside (bgl) operon of Escherichia coli K-12: nucleotide sequence, genetic organization, and possible evolutionary relationship to regulatory components of two Bacillus subtilis genes." Schnetz K., Toloczyki C., Rak B. J. Bacteriol. 169:2579-2590(1987) [PubMed: 3034860] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R. Genomics 16:551-561(1993) [PubMed: 7686882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Nucleotide sequence of bglC, the gene specifying enzymeIIbgl of the PEP:sugar phosphotransferase system in Escherichia coli K12, and overexpression of the gene product." Bramley H.F., Kornberg H.L. J. Gen. Microbiol. 133:563-573(1987) [PubMed: 3309161] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19. Strain: K12.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M16487 Genomic DNA. Translation: AAA23511.1. L10328 Genomic DNA. Translation: AAA62072.1. U00096 Genomic DNA. Translation: AAC76744.1. AP009048 Genomic DNA. Translation: BAE77567.1. M15746 Genomic DNA. Translation: AAA83838.1.
PIR	B65175.
RefSeq	AP_004066.1. NP_418177.1.
3D structure databases
HSSP	HSSP built from PDB template 1GNX based on UniProtKB Q59976.
ModBase	Search...
Genome annotation databases
GeneID	948234.
GenomeReviews	Gene locus b3721 in contig U00096_GR. Gene locus JW3699 in contig AP009048_GR.
KEGG	ecj:JW3699. eco:b3721.
Organism-specific databases
EchoBASE	EB0112.
EcoGene	EG10114. bglB.
CMR	Search...
Phylogenomic databases
HOGENOM	P11988.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10114-MON. MetaCyc:EG10114-MON.
Family and domain databases
InterPro	IPR001360. Glyco_hydro_1. IPR013781. Glyco_hydro_sub_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGLB_ECOLI

Accession

Primary (citable) accession number: P11988
Secondary accession number(s): P78124, Q2M839

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	July 1, 1993
Last modified:	November 25, 2008
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)