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Protein

6-phospho-beta-glucosidase BglB

Gene

bglB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of phosphorylated beta-glucosides into glucose-6-phosphate (G-6-P) and aglycone. It has a high affinity for phosphorylated aromatic beta-glucosides (p-nitrophenyl-beta-glucoside, phenyl beta-glucoside, arbutin and phosphorylated salicin), and a low affinity for phosphorylated beta-methyl-glucoside.1 Publication

Miscellaneous

Part of the cryptic bglGFBH operon.

Catalytic activityi

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei172Proton donorSequence analysis1
Active sitei361NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:EG10114-MONOMER.
MetaCyc:EG10114-MONOMER.
SABIO-RKiP11988.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-beta-glucosidase BglB (EC:3.2.1.86)
Alternative name(s):
Phospho-beta-glucosidase B
Gene namesi
Name:bglB
Ordered Locus Names:b3721, JW3699
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10114. bglB.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000638981 – 4706-phospho-beta-glucosidase BglBAdd BLAST470

Proteomic databases

PaxDbiP11988.
PRIDEiP11988.

Interactioni

Protein-protein interaction databases

BioGridi4261195. 13 interactors.
DIPiDIP-9214N.
IntActiP11988. 3 interactors.
STRINGi316385.ECDH10B_3908.

Structurei

3D structure databases

ProteinModelPortaliP11988.
SMRiP11988.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Phylogenomic databases

eggNOGiENOG4105CS2. Bacteria.
COG2723. LUCA.
HOGENOMiHOG000088631.
InParanoidiP11988.
KOiK01223.
PhylomeDBiP11988.

Family and domain databases

InterProiView protein in InterPro
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR017853. Glycoside_hydrolase_SF.
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiView protein in Pfam
PF00232. Glyco_hydro_1. 1 hit.
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiView protein in PROSITE
PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P11988-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAFPETFLW GGATAANQVE GAWQEDGKGI STSDLQPHGV MGKMEPRILG
60 70 80 90 100
KENIKDVAID FYHRYPEDIA LFAEMGFTCL RISIAWARIF PQGDEVEPNE
110 120 130 140 150
AGLAFYDRLF DEMAQAGIKP LVTLSHYEMP YGLVKNYGGW ANRAVIDHFE
160 170 180 190 200
HYARTVFTRY QHKVALWLTF NEINMSLHAP FTGVGLAEES GEAEVYQAIH
210 220 230 240 250
HQLVASARAV KACHSLLPEA KIGNMLLGGL VYPLTCQPQD MLQAMEENRR
260 270 280 290 300
WMFFGDVQAR GQYPGYMQRF FRDHNITIEM TESDAEDLKH TVDFISFSYY
310 320 330 340 350
MTGCVSHDES INKNAQGNIL NMIPNPHLKS SEWGWQIDPV GLRVLLNTLW
360 370 380 390 400
DRYQKPLFIV ENGLGAKDSV EADGSIQDDY RIAYLNDHLV QVNEAIADGV
410 420 430 440 450
DIMGYTSWGP IDLVSASHSQ MSKRYGFIYV DRDDNGEGSL TRTRKKSFGW
460 470
YAEVIKTRGL SLKKITIKAP
Length:470
Mass (Da):53,161
Last modified:July 1, 1993 - v2
Checksum:i2B8422B20D7E5C98
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti147D → G in AAA23511 (PubMed:3034860).Curated1
Sequence conflicti449 – 451GWY → RMVC in AAA23511 (PubMed:3034860).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16487 Genomic DNA. Translation: AAA23511.1.
L10328 Genomic DNA. Translation: AAA62072.1.
U00096 Genomic DNA. Translation: AAC76744.1.
AP009048 Genomic DNA. Translation: BAE77567.1.
M15746 Genomic DNA. Translation: AAA83838.1.
PIRiB65175.
RefSeqiNP_418177.1. NC_000913.3.
WP_000643228.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76744; AAC76744; b3721.
BAE77567; BAE77567; BAE77567.
GeneIDi948234.
KEGGiecj:JW3699.
eco:b3721.
PATRICifig|1411691.4.peg.2980.

Similar proteinsi

Entry informationi

Entry nameiBGLB_ECOLI
AccessioniPrimary (citable) accession number: P11988
Secondary accession number(s): P78124, Q2M839
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 1, 1993
Last modified: August 30, 2017
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families