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P11986

- INO1_YEAST

UniProt

P11986 - INO1_YEAST

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Protein

Inositol-3-phosphate synthase

Gene
INO1, YJL153C, J0610
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate.

Cofactori

NAD.

Pathwayi

GO - Molecular functioni

  1. inositol-3-phosphate synthase activity Source: UniProtKB

GO - Biological processi

  1. inositol biosynthetic process Source: UniProtKB
  2. phospholipid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Inositol biosynthesis, Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciYEAST:YJL153C-MONOMER.
ReactomeiREACT_188936. Synthesis of IP2, IP, and Ins in the cytosol.
SABIO-RKP11986.
UniPathwayiUPA00823; UER00787.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol-3-phosphate synthase (EC:5.5.1.4)
Short name:
MIP synthase
Alternative name(s):
Myo-inositol 1-phosphate synthase
Short name:
IPS
Short name:
MI-1-P synthase
Gene namesi
Name:INO1
Ordered Locus Names:YJL153C
ORF Names:J0610
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL153c.
SGDiS000003689. INO1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 533533Inositol-3-phosphate synthasePRO_0000195185Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei368 – 3681Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP11986.
PaxDbiP11986.
PRIDEiP11986.

Expressioni

Gene expression databases

GenevestigatoriP11986.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself1EBI-9257,EBI-9257
AAT2P235421EBI-9257,EBI-2002
ADE17P380091EBI-9257,EBI-14223
ALO1P547831EBI-9257,EBI-2519
ARO8P530901EBI-9257,EBI-2042933
ARP2P323811EBI-9257,EBI-2927
BNA3P470391EBI-9257,EBI-25893
CAR2P079911EBI-9257,EBI-12430
CHC1P221371EBI-9257,EBI-4766
CYS4P325821EBI-9257,EBI-4167
EFT1P323241EBI-9257,EBI-6333
GCY1P140651EBI-9257,EBI-7505
GET3Q121541EBI-9257,EBI-2989
GLN1P322881EBI-9257,EBI-7665
HSP12P229431EBI-9257,EBI-8548
HUG1Q6Q5K61EBI-9257,EBI-392766
IPP1P008171EBI-9257,EBI-9338
MBF1O144671EBI-9257,EBI-2043025
MET13P531281EBI-9257,EBI-11572
OYE2Q035581EBI-9257,EBI-12729
RNR1P215241EBI-9257,EBI-15234
RNR2P099381EBI-9257,EBI-15240
SEC27P418111EBI-9257,EBI-4898
SRV2P175551EBI-9257,EBI-4024
SSD1P242761EBI-9257,EBI-18153
VAS1P07806-11EBI-9257,EBI-1009942From a different organism.
VMA4P222031EBI-9257,EBI-20268
VMA8P326101EBI-9257,EBI-20264

Protein-protein interaction databases

BioGridi33607. 51 interactions.
DIPiDIP-5687N.
IntActiP11986. 20 interactions.
MINTiMINT-551495.
STRINGi4932.YJL153C.

Structurei

Secondary structure

1
533
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 144
Beta strandi19 – 224
Beta strandi25 – 3915
Beta strandi43 – 5917
Beta strandi64 – 718
Turni72 – 743
Helixi76 – 8914
Beta strandi94 – 963
Beta strandi99 – 1013
Helixi109 – 1124
Beta strandi114 – 1196
Beta strandi121 – 1233
Beta strandi125 – 1295
Helixi130 – 1323
Helixi139 – 1413
Beta strandi142 – 1476
Helixi154 – 1618
Helixi166 – 17611
Helixi188 – 1903
Helixi193 – 1986
Beta strandi207 – 2093
Helixi216 – 23318
Beta strandi239 – 2435
Turni255 – 2573
Beta strandi258 – 2603
Helixi261 – 2699
Helixi277 – 28711
Beta strandi292 – 2943
Beta strandi296 – 2983
Helixi303 – 31210
Beta strandi316 – 3216
Helixi325 – 33814
Beta strandi342 – 35211
Helixi355 – 3606
Helixi363 – 37513
Helixi378 – 3825
Turni385 – 3873
Turni390 – 3923
Beta strandi397 – 4048
Helixi406 – 4083
Beta strandi411 – 42111
Helixi423 – 4253
Beta strandi427 – 43711
Helixi438 – 45720
Beta strandi459 – 4635
Beta strandi466 – 4683
Helixi482 – 4876
Beta strandi488 – 4903
Helixi503 – 51715
Helixi528 – 5314

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JKFX-ray2.40A/B1-533[»]
1JKIX-ray2.20A/B1-533[»]
1LA2X-ray2.65A/B/C/D1-533[»]
1P1FX-ray2.60A/B1-533[»]
1P1HX-ray1.95A/B/C/D1-533[»]
1P1IX-ray2.40A/B1-533[»]
1P1JX-ray1.70A/B1-533[»]
1P1KX-ray2.10A/B1-533[»]
1RM0X-ray2.05A/B1-533[»]
ProteinModelPortaliP11986.
SMRiP11986. Positions 9-533.

Miscellaneous databases

EvolutionaryTraceiP11986.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1260.
GeneTreeiENSGT00390000018395.
HOGENOMiHOG000013469.
KOiK01858.
OMAiNPVLYAP.
OrthoDBiEOG7DZ8V4.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002587. Myo-inos-1-P_Synthase.
IPR013021. Myo-inos-1-P_Synthase_GAPDH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11510. PTHR11510. 1 hit.
PfamiPF01658. Inos-1-P_synth. 1 hit.
PF07994. NAD_binding_5. 1 hit.
[Graphical view]
PIRSFiPIRSF015578. Myoinos-ppht_syn. 1 hit.

Sequencei

Sequence statusi: Complete.

P11986-1 [UniParc]FASTAAdd to Basket

« Hide

MTEDNIAPIT SVKVVTDKCT YKDNELLTKY SYENAVVTKT ASGRFDVTPT    50
VQDYVFKLDL KKPEKLGIML IGLGGNNGST LVASVLANKH NVEFQTKEGV 100
KQPNYFGSMT QCSTLKLGID AEGNDVYAPF NSLLPMVSPN DFVVSGWDIN 150
NADLYEAMQR SQVLEYDLQQ RLKAKMSLVK PLPSIYYPDF IAANQDERAN 200
NCINLDEKGN VTTRGKWTHL QRIRRDIQNF KEENALDKVI VLWTANTERY 250
VEVSPGVNDT MENLLQSIKN DHEEIAPSTI FAAASILEGV PYINGSPQNT 300
FVPGLVQLAE HEGTFIAGDD LKSGQTKLKS VLAQFLVDAG IKPVSIASYN 350
HLGNNDGYNL SAPKQFRSKE ISKSSVIDDI IASNDILYND KLGKKVDHCI 400
VIKYMKPVGD SKVAMDEYYS ELMLGGHNRI SIHNVCEDSL LATPLIIDLL 450
VMTEFCTRVS YKKVDPVKED AGKFENFYPV LTFLSYWLKA PLTRPGFHPV 500
NGLNKQRTAL ENFLRLLIGL PSQNELRFEE RLL 533
Length:533
Mass (Da):59,642
Last modified:December 6, 2005 - v3
Checksum:i282E2AE5D156289D
GO

Sequence cautioni

The sequence CAA60802.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA89448.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141V → RL1 Publication
Sequence conflicti14 – 141V → RL1 Publication
Sequence conflicti60 – 601L → FE1 Publication
Sequence conflicti60 – 601L → FE1 Publication
Sequence conflicti66 – 9328LGIML…KHNVE → TRNYAHWVRWLQQWLTLWPR YWRISTMWS1 PublicationAdd
BLAST
Sequence conflicti66 – 9328LGIML…KHNVE → TRNYAHWVRWLQQWLTLWPR YWRISTMWS1 PublicationAdd
BLAST
Sequence conflicti98 – 981E → AK1 Publication
Sequence conflicti98 – 981E → AK1 Publication
Sequence conflicti140 – 1412ND → KH1 Publication
Sequence conflicti140 – 1412ND → KH1 Publication
Sequence conflicti201 – 2011N → Q1 Publication
Sequence conflicti201 – 2011N → Q1 Publication
Sequence conflicti444 – 4441P → A1 Publication
Sequence conflicti444 – 4441P → A1 Publication
Sequence conflicti476 – 49722NFYPV…TRPGF → ELLSSFNLLELLVKSSINKN QDL in AAA66310. 1 PublicationAdd
BLAST
Sequence conflicti524 – 53310NELRFEERLL → KRTKIRREIVVISFQRLSFS FSAYL in AAA34706. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L23520 Genomic DNA. Translation: AAA34706.1.
J04453 Genomic DNA. Translation: AAA66310.1.
X87371 Genomic DNA. Translation: CAA60802.1. Different initiation.
Z49428 Genomic DNA. Translation: CAA89448.1. Different initiation.
BK006943 Genomic DNA. Translation: DAA08650.1.
PIRiS55160. A30902.
RefSeqiNP_012382.2. NM_001181586.1.

Genome annotation databases

EnsemblFungiiYJL153C; YJL153C; YJL153C.
GeneIDi853288.
KEGGisce:YJL153C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L23520 Genomic DNA. Translation: AAA34706.1 .
J04453 Genomic DNA. Translation: AAA66310.1 .
X87371 Genomic DNA. Translation: CAA60802.1 . Different initiation.
Z49428 Genomic DNA. Translation: CAA89448.1 . Different initiation.
BK006943 Genomic DNA. Translation: DAA08650.1 .
PIRi S55160. A30902.
RefSeqi NP_012382.2. NM_001181586.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1JKF X-ray 2.40 A/B 1-533 [» ]
1JKI X-ray 2.20 A/B 1-533 [» ]
1LA2 X-ray 2.65 A/B/C/D 1-533 [» ]
1P1F X-ray 2.60 A/B 1-533 [» ]
1P1H X-ray 1.95 A/B/C/D 1-533 [» ]
1P1I X-ray 2.40 A/B 1-533 [» ]
1P1J X-ray 1.70 A/B 1-533 [» ]
1P1K X-ray 2.10 A/B 1-533 [» ]
1RM0 X-ray 2.05 A/B 1-533 [» ]
ProteinModelPortali P11986.
SMRi P11986. Positions 9-533.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33607. 51 interactions.
DIPi DIP-5687N.
IntActi P11986. 20 interactions.
MINTi MINT-551495.
STRINGi 4932.YJL153C.

Proteomic databases

MaxQBi P11986.
PaxDbi P11986.
PRIDEi P11986.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL153C ; YJL153C ; YJL153C .
GeneIDi 853288.
KEGGi sce:YJL153C.

Organism-specific databases

CYGDi YJL153c.
SGDi S000003689. INO1.

Phylogenomic databases

eggNOGi COG1260.
GeneTreei ENSGT00390000018395.
HOGENOMi HOG000013469.
KOi K01858.
OMAi NPVLYAP.
OrthoDBi EOG7DZ8V4.

Enzyme and pathway databases

UniPathwayi UPA00823 ; UER00787 .
BioCyci YEAST:YJL153C-MONOMER.
Reactomei REACT_188936. Synthesis of IP2, IP, and Ins in the cytosol.
SABIO-RK P11986.

Miscellaneous databases

EvolutionaryTracei P11986.
NextBioi 973591.
PROi P11986.

Gene expression databases

Genevestigatori P11986.

Family and domain databases

Gene3Di 3.40.50.720. 2 hits.
InterProi IPR002587. Myo-inos-1-P_Synthase.
IPR013021. Myo-inos-1-P_Synthase_GAPDH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11510. PTHR11510. 1 hit.
Pfami PF01658. Inos-1-P_synth. 1 hit.
PF07994. NAD_binding_5. 1 hit.
[Graphical view ]
PIRSFi PIRSF015578. Myoinos-ppht_syn. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Biosynthesis of inositol in yeast. Primary structure of myo-inositol-1-phosphate synthase (EC 5.5.1.4) and functional analysis of its structural gene, the INO1 locus."
    Dean-Johnson M., Henry S.A.
    J. Biol. Chem. 264:1274-1283(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: ATCC 204510 / AB320.
  2. "Comparison of INO1 gene sequences and products in Candida albicans and Saccharomyces cerevisiae."
    Klig L.S., Zobel P.A., Devry C.G., Losberger C.
    Yeast 10:789-800(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: AB322.
  3. "Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI."
    Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D.
    Yeast 12:787-797(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-368, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "The crystal structure and mechanism of 1-L-myo-inositol-1-phosphate synthase."
    Stein A.J., Geiger J.H.
    J. Biol. Chem. 277:9484-9491(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).

Entry informationi

Entry nameiINO1_YEAST
AccessioniPrimary (citable) accession number: P11986
Secondary accession number(s): D6VW34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: December 6, 2005
Last modified: September 3, 2014
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

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