Inositol-3-phosphate synthase - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P11986 (INO1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 118. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Inositol-3-phosphate synthase
Short name=MIP synthase
EC=5.5.1.4

Alternative name(s):
Myo-inositol-1-phosphate synthase
Short name=IPS
Short name=MI-1-P synthase

Gene names

Name:	INO1
Ordered Locus Names:	YJL153C
ORF Names:	J0610

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate.
Cofactor	NAD.
Pathway	Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 1/2.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the myo-inositol-1-phosphate synthase family.
Sequence caution	The sequence CAA60802.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA89448.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Inositol biosynthesis Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Isomerase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	inositol biosynthetic process Inferred from direct assay Ref.3. Source: UniProtKB phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay Ref.3. Source: UniProtKB
Molecular function	inositol-3-phosphate synthase activity Inferred from direct assay Ref.3. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-9257,EBI-9257
AAT2	P23542	1	EBI-9257,EBI-2002
ADE17	P38009	1	EBI-9257,EBI-14223
ALO1	P54783	1	EBI-9257,EBI-2519
ARO8	P53090	1	EBI-9257,EBI-2042933
ARP2	P32381	1	EBI-9257,EBI-2927
BNA3	P47039	1	EBI-9257,EBI-25893
CAR2	P07991	1	EBI-9257,EBI-12430
CHC1	P22137	1	EBI-9257,EBI-4766
CYS4	P32582	1	EBI-9257,EBI-4167
EFT1	P32324	1	EBI-9257,EBI-6333
GCY1	P14065	1	EBI-9257,EBI-7505
GET3	Q12154	1	EBI-9257,EBI-2989
GLN1	P32288	1	EBI-9257,EBI-7665
HSP12	P22943	1	EBI-9257,EBI-8548
HUG1	Q6Q5K6	1	EBI-9257,EBI-392766
IPP1	P00817	1	EBI-9257,EBI-9338
MBF1	O14467	1	EBI-9257,EBI-2043025
MET13	P53128	1	EBI-9257,EBI-11572
OYE2	Q03558	1	EBI-9257,EBI-12729
RNR1	P21524	1	EBI-9257,EBI-15234
RNR2	P09938	1	EBI-9257,EBI-15240
SEC27	P41811	1	EBI-9257,EBI-4898
SRV2	P17555	1	EBI-9257,EBI-4024
SSD1	P24276	1	EBI-9257,EBI-18153
VAS1	P07806-1	1	EBI-9257,EBI-1009942	From a different organism.
VMA4	P22203	1	EBI-9257,EBI-20268
VMA8	P32610	1	EBI-9257,EBI-20264

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 533

533

Inositol-3-phosphate synthase

PRO_0000195185

Experimental info

Sequence conflict

V → RL Ref.1

Sequence conflict

V → RL Ref.2

Sequence conflict

L → FE Ref.1

Sequence conflict

L → FE Ref.2

Sequence conflict

66 – 93

LGIML…KHNVE → TRNYAHWVRWLQQWLTLWPR YWRISTMWS Ref.1

Sequence conflict

66 – 93

LGIML…KHNVE → TRNYAHWVRWLQQWLTLWPR YWRISTMWS Ref.2

Sequence conflict

E → AK Ref.1

Sequence conflict

E → AK Ref.2

Sequence conflict

140 – 141

ND → KH Ref.1

Sequence conflict

140 – 141

ND → KH Ref.2

Sequence conflict

201

N → Q Ref.1

Sequence conflict

201

N → Q Ref.2

Sequence conflict

444

P → A Ref.1

Sequence conflict

444

P → A Ref.2

Sequence conflict

476 – 497

NFYPV…TRPGF → ELLSSFNLLELLVKSSINKN QDL in AAA66310. Ref.1

Sequence conflict

524 – 533

NELRFEERLL → KRTKIRREIVVISFQRLSFS FSAYL in AAA34706. Ref.1

Secondary structure

533

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P11986 [UniParc].

Last modified December 6, 2005. Version 3.
Checksum: 282E2AE5D156289D
Show »

FASTA

533

59,642

        10         20         30         40         50         60 
MTEDNIAPIT SVKVVTDKCT YKDNELLTKY SYENAVVTKT ASGRFDVTPT VQDYVFKLDL 

        70         80         90        100        110        120 
KKPEKLGIML IGLGGNNGST LVASVLANKH NVEFQTKEGV KQPNYFGSMT QCSTLKLGID 

       130        140        150        160        170        180 
AEGNDVYAPF NSLLPMVSPN DFVVSGWDIN NADLYEAMQR SQVLEYDLQQ RLKAKMSLVK 

       190        200        210        220        230        240 
PLPSIYYPDF IAANQDERAN NCINLDEKGN VTTRGKWTHL QRIRRDIQNF KEENALDKVI 

       250        260        270        280        290        300 
VLWTANTERY VEVSPGVNDT MENLLQSIKN DHEEIAPSTI FAAASILEGV PYINGSPQNT 

       310        320        330        340        350        360 
FVPGLVQLAE HEGTFIAGDD LKSGQTKLKS VLAQFLVDAG IKPVSIASYN HLGNNDGYNL 

       370        380        390        400        410        420 
SAPKQFRSKE ISKSSVIDDI IASNDILYND KLGKKVDHCI VIKYMKPVGD SKVAMDEYYS 

       430        440        450        460        470        480 
ELMLGGHNRI SIHNVCEDSL LATPLIIDLL VMTEFCTRVS YKKVDPVKED AGKFENFYPV 

       490        500        510        520        530 
LTFLSYWLKA PLTRPGFHPV NGLNKQRTAL ENFLRLLIGL PSQNELRFEE RLL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biosynthesis of inositol in yeast. Primary structure of myo-inositol-1-phosphate synthase (EC 5.5.1.4) and functional analysis of its structural gene, the INO1 locus." Dean-Johnson M., Henry S.A. J. Biol. Chem. 264:1274-1283(1989) [PubMed: 2642902] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 204510 / AB320.
[2]	"Comparison of INO1 gene sequences and products in Candida albicans and Saccharomyces cerevisiae." Klig L.S., Zobel P.A., Devry C.G., Losberger C. Yeast 10:789-800(1994) [PubMed: 7975896] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AB322.
[3]	"Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI." Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D. Yeast 12:787-797(1996) [PubMed: 8813765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"The crystal structure and mechanism of 1-L-myo-inositol-1-phosphate synthase." Stein A.J., Geiger J.H. J. Biol. Chem. 277:9484-9491(2002) [PubMed: 11779862] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L23520 Genomic DNA. Translation: AAA34706.1.
J04453 Genomic DNA. Translation: AAA66310.1.
X87371 Genomic DNA. Translation: CAA60802.1. Different initiation.
Z49428 Genomic DNA. Translation: CAA89448.1. Different initiation.
BK006943 Genomic DNA. Translation: DAA08650.1.

PIR

A30902. S55160.

RefSeq

NP_012382.2. NM_001181586.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JKF	X-ray	2.40	A/B	1-533	[»]
1JKI	X-ray	2.20	A/B	1-533	[»]
1LA2	X-ray	2.65	A/B/C/D	1-533	[»]
1P1F	X-ray	2.60	A/B	1-533	[»]
1P1H	X-ray	1.95	A/B/C/D	1-533	[»]
1P1I	X-ray	2.40	A/B	1-533	[»]
1P1J	X-ray	1.70	A/B	1-533	[»]
1P1K	X-ray	2.10	A/B	1-533	[»]
1RM0	X-ray	2.05	A/B	1-533	[»]

ProteinModelPortal

P11986.

SMR

P11986. Positions 9-533.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5687N.

IntAct

P11986. 29 interactions.

MINT

MINT-551495.

STRING

P11986.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YJL153C; YJL153C; YJL153C.

GeneID

853288.

KEGG

sce:YJL153C.

NMPDR

fig|4932.3.peg.3347.

Organism-specific databases

CYGD

YJL153c.

SGD

S000003689. INO1.

Phylogenomic databases

eggNOG

fuNOG04565.

GeneTree

EFGT00050000002318.

HOGENOM

HBG316335.

OMA

NPVLYAP.

OrthoDB

EOG441TKW.

Gene expression databases

ArrayExpress

P11986.

Genevestigator

P11986.

GermOnline

YJL153C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR002587. Myo-inos-1-P_Synthase.
IPR013021. Myo-inos-1-P_Synthase_GAPDH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.

K01858.

PANTHER

PTHR11510. Inos-1-P_synth. 1 hit.

Pfam

PF01658. Inos-1-P_synth. 1 hit.
PF07994. NAD_binding_5. 1 hit.
[Graphical view]

PIRSF

PIRSF015578. Myoinos-ppht_syn. 1 hit.

ProtoNet

Search...

Other

NextBio

973591.

Entry information

Entry name

INO1_YEAST

Accession

Primary (citable) accession number: P11986
Secondary accession number(s): D6VW34

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	December 6, 2005
Last modified:	December 14, 2011
This is version 118 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents