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P11986 (INO1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inositol-3-phosphate synthase

Short name=MIP synthase
EC=5.5.1.4
Alternative name(s):
Myo-inositol 1-phosphate synthase
Short name=IPS
Short name=MI-1-P synthase
Gene names
Name:INO1
Ordered Locus Names:YJL153C
ORF Names:J0610
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length533 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate.

Cofactor

NAD.

Pathway

Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 1/2.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the myo-inositol 1-phosphate synthase family.

Sequence caution

The sequence CAA60802.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA89448.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 533533Inositol-3-phosphate synthase
PRO_0000195185

Amino acid modifications

Modified residue3681Phosphoserine Ref.6

Experimental info

Sequence conflict141V → RL Ref.1
Sequence conflict141V → RL Ref.2
Sequence conflict601L → FE Ref.1
Sequence conflict601L → FE Ref.2
Sequence conflict66 – 9328LGIML…KHNVE → TRNYAHWVRWLQQWLTLWPR YWRISTMWS Ref.1
Sequence conflict66 – 9328LGIML…KHNVE → TRNYAHWVRWLQQWLTLWPR YWRISTMWS Ref.2
Sequence conflict981E → AK Ref.1
Sequence conflict981E → AK Ref.2
Sequence conflict140 – 1412ND → KH Ref.1
Sequence conflict140 – 1412ND → KH Ref.2
Sequence conflict2011N → Q Ref.1
Sequence conflict2011N → Q Ref.2
Sequence conflict4441P → A Ref.1
Sequence conflict4441P → A Ref.2
Sequence conflict476 – 49722NFYPV…TRPGF → ELLSSFNLLELLVKSSINKN QDL in AAA66310. Ref.1
Sequence conflict524 – 53310NELRFEERLL → KRTKIRREIVVISFQRLSFS FSAYL in AAA34706. Ref.1

Secondary structure

.............................................................................................. 533
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P11986 [UniParc].

Last modified December 6, 2005. Version 3.
Checksum: 282E2AE5D156289D

FASTA53359,642
        10         20         30         40         50         60 
MTEDNIAPIT SVKVVTDKCT YKDNELLTKY SYENAVVTKT ASGRFDVTPT VQDYVFKLDL 

        70         80         90        100        110        120 
KKPEKLGIML IGLGGNNGST LVASVLANKH NVEFQTKEGV KQPNYFGSMT QCSTLKLGID 

       130        140        150        160        170        180 
AEGNDVYAPF NSLLPMVSPN DFVVSGWDIN NADLYEAMQR SQVLEYDLQQ RLKAKMSLVK 

       190        200        210        220        230        240 
PLPSIYYPDF IAANQDERAN NCINLDEKGN VTTRGKWTHL QRIRRDIQNF KEENALDKVI 

       250        260        270        280        290        300 
VLWTANTERY VEVSPGVNDT MENLLQSIKN DHEEIAPSTI FAAASILEGV PYINGSPQNT 

       310        320        330        340        350        360 
FVPGLVQLAE HEGTFIAGDD LKSGQTKLKS VLAQFLVDAG IKPVSIASYN HLGNNDGYNL 

       370        380        390        400        410        420 
SAPKQFRSKE ISKSSVIDDI IASNDILYND KLGKKVDHCI VIKYMKPVGD SKVAMDEYYS 

       430        440        450        460        470        480 
ELMLGGHNRI SIHNVCEDSL LATPLIIDLL VMTEFCTRVS YKKVDPVKED AGKFENFYPV 

       490        500        510        520        530 
LTFLSYWLKA PLTRPGFHPV NGLNKQRTAL ENFLRLLIGL PSQNELRFEE RLL 

« Hide

References

« Hide 'large scale' references
[1]"Biosynthesis of inositol in yeast. Primary structure of myo-inositol-1-phosphate synthase (EC 5.5.1.4) and functional analysis of its structural gene, the INO1 locus."
Dean-Johnson M., Henry S.A.
J. Biol. Chem. 264:1274-1283(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: ATCC 204510 / AB320.
[2]"Comparison of INO1 gene sequences and products in Candida albicans and Saccharomyces cerevisiae."
Klig L.S., Zobel P.A., Devry C.G., Losberger C.
Yeast 10:789-800(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: AB322.
[3]"Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI."
Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D.
Yeast 12:787-797(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-368, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"The crystal structure and mechanism of 1-L-myo-inositol-1-phosphate synthase."
Stein A.J., Geiger J.H.
J. Biol. Chem. 277:9484-9491(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L23520 Genomic DNA. Translation: AAA34706.1.
J04453 Genomic DNA. Translation: AAA66310.1.
X87371 Genomic DNA. Translation: CAA60802.1. Different initiation.
Z49428 Genomic DNA. Translation: CAA89448.1. Different initiation.
BK006943 Genomic DNA. Translation: DAA08650.1.
PIRA30902. S55160.
RefSeqNP_012382.2. NM_001181586.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1JKFX-ray2.40A/B1-533[»]
1JKIX-ray2.20A/B1-533[»]
1LA2X-ray2.65A/B/C/D1-533[»]
1P1FX-ray2.60A/B1-533[»]
1P1HX-ray1.95A/B/C/D1-533[»]
1P1IX-ray2.40A/B1-533[»]
1P1JX-ray1.70A/B1-533[»]
1P1KX-ray2.10A/B1-533[»]
1RM0X-ray2.05A/B1-533[»]
ProteinModelPortalP11986.
SMRP11986. Positions 9-533.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33607. 51 interactions.
DIPDIP-5687N.
IntActP11986. 20 interactions.
MINTMINT-551495.
STRING4932.YJL153C.

Proteomic databases

MaxQBP11986.
PaxDbP11986.
PRIDEP11986.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL153C; YJL153C; YJL153C.
GeneID853288.
KEGGsce:YJL153C.

Organism-specific databases

CYGDYJL153c.
SGDS000003689. INO1.

Phylogenomic databases

eggNOGCOG1260.
GeneTreeENSGT00390000018395.
HOGENOMHOG000013469.
KOK01858.
OMANPVLYAP.
OrthoDBEOG7DZ8V4.

Enzyme and pathway databases

BioCycYEAST:YJL153C-MONOMER.
SABIO-RKP11986.
UniPathwayUPA00823; UER00787.

Gene expression databases

GenevestigatorP11986.

Family and domain databases

Gene3D3.40.50.720. 2 hits.
InterProIPR002587. Myo-inos-1-P_Synthase.
IPR013021. Myo-inos-1-P_Synthase_GAPDH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11510. PTHR11510. 1 hit.
PfamPF01658. Inos-1-P_synth. 1 hit.
PF07994. NAD_binding_5. 1 hit.
[Graphical view]
PIRSFPIRSF015578. Myoinos-ppht_syn. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP11986.
NextBio973591.
PROP11986.

Entry information

Entry nameINO1_YEAST
AccessionPrimary (citable) accession number: P11986
Secondary accession number(s): D6VW34
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: December 6, 2005
Last modified: May 14, 2014
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways