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Protein

T-complex protein 1 subunit alpha

Gene

Tcp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-390471. Association of TriC/CCT with target proteins during biosynthesis.
R-MMU-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Names & Taxonomyi

Protein namesi
Recommended name:
T-complex protein 1 subunit alpha
Short name:
TCP-1-alpha
Alternative name(s):
CCT-alpha
Tailless complex polypeptide 1A
Short name:
TCP-1-A
Tailless complex polypeptide 1B
Short name:
TCP-1-B
Gene namesi
Name:Tcp1
Synonyms:Cct1, Ccta
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:98535. Tcp1.

Subcellular locationi

GO - Cellular componenti

  • acrosomal vesicle Source: MGI
  • cell body Source: MGI
  • centrosome Source: MGI
  • chaperonin-containing T-complex Source: MGI
  • extracellular exosome Source: MGI
  • Golgi apparatus Source: MGI
  • microtubule Source: MGI
  • microtubule organizing center Source: MGI
  • myelin sheath Source: UniProtKB
  • nuclear heterochromatin Source: MGI
  • pericentriolar material Source: MGI
  • zona pellucida receptor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001283051 – 556T-complex protein 1 subunit alphaAdd BLAST556

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei6PhosphoserineBy similarity1
Modified residuei181PhosphotyrosineBy similarity1
Modified residuei199N6-acetyllysineBy similarity1
Modified residuei400N6-acetyllysineCombined sources1
Modified residuei494N6-acetyllysineCombined sources1
Modified residuei544PhosphoserineCombined sources1
Modified residuei551PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP11983.
MaxQBiP11983.
PaxDbiP11983.
PeptideAtlasiP11983.
PRIDEiP11983.

2D gel databases

REPRODUCTION-2DPAGEIPI00459493.
P11983.

PTM databases

iPTMnetiP11983.
PhosphoSitePlusiP11983.

Expressioni

Gene expression databases

BgeeiENSMUSG00000068039.
CleanExiMM_TCP1.
ExpressionAtlasiP11983. baseline and differential.
GenevisibleiP11983. MM.

Interactioni

Subunit structurei

Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Component of the BBS/CCT complex composed at least of MKKS, BBS10, BBS12, TCP1, CCT2, CCT3, CCT4, CCT5 AND CCT8 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204044. 14 interactors.
DIPiDIP-32342N.
IntActiP11983. 10 interactors.
MINTiMINT-1862852.
STRINGi10090.ENSMUSP00000116108.

Structurei

3D structure databases

ProteinModelPortaliP11983.
SMRiP11983.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TCP-1 chaperonin family.Curated

Phylogenomic databases

eggNOGiKOG0360. Eukaryota.
COG0459. LUCA.
GeneTreeiENSGT00550000074878.
HOVERGENiHBG001052.
InParanoidiP11983.
KOiK09493.
OMAiHYLAQEG.
OrthoDBiEOG091G04HL.
TreeFamiTF106331.

Family and domain databases

CDDicd03335. TCP1_alpha. 1 hit.
Gene3Di1.10.560.10. 2 hits.
3.30.260.10. 2 hits.
3.50.7.10. 1 hit.
InterProiIPR012715. Chap_CCT_alpha.
IPR017998. Chaperone_TCP-1.
IPR002194. Chaperonin_TCP-1_CS.
IPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027413. GROEL-like_equatorial.
IPR027410. TCP-1-like_intermed.
[Graphical view]
PfamiPF00118. Cpn60_TCP1. 1 hit.
[Graphical view]
PRINTSiPR00304. TCOMPLEXTCP1.
SUPFAMiSSF52029. SSF52029. 1 hit.
TIGRFAMsiTIGR02340. chap_CCT_alpha. 1 hit.
PROSITEiPS00750. TCP1_1. 1 hit.
PS00751. TCP1_2. 1 hit.
PS00995. TCP1_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P11983-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGPLSVFGD RSTGEAVRSQ NVMAAASIAN IVKSSFGPVG LDKMLVDDIG
60 70 80 90 100
DVTITNDGAT ILKLLEVEHP AAKVLCELAD LQDKEVGDGT TSVVIIAAEL
110 120 130 140 150
LKNADELVKQ KIHPTSVISG YRLACKEAVR YINENLIINT DELGRDCLIN
160 170 180 190 200
AAKTSMSSKI IGINGDYFAN MVVDAVLAVK YTDARGQPRY PVNSVNILKA
210 220 230 240 250
HGRSQIESML INGYALNCVV GSQGMPKRIV NAKIACLDFS LQKTKMKLGV
260 270 280 290 300
QVVITDPEKL DQIRQRESDI TKERIQKILA TGANVILTTG GIDDMYLKYF
310 320 330 340 350
VEAGAMAVRR VLKRDLKHVA KASGASILST LANLEGEETF EVTMLGQAEE
360 370 380 390 400
VVQERICDDE LILIKNTKAR TSASIILRGA NDFMCDEMER SLHDALCVVK
410 420 430 440 450
RVLELKSVVP GGGAVEAALS IYLENYATSM GSREQLAIAE FARSLLVIPN
460 470 480 490 500
TLAVNAAQDS TDLVAKLRAF HNEAQVNPER KNLKWIGLDL VHGKPRDNKQ
510 520 530 540 550
AGVFEPTIVK VKSLKFATEA AITILRIDDL IKLHPESKDD KHGSYENAVH

SGALDD
Length:556
Mass (Da):60,449
Last modified:October 1, 1996 - v3
Checksum:i48F2387BE0F909A4
GO
Isoform 2 (identifier: P11983-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.
     50-50: G → M

Note: No experimental confirmation available.
Show »
Length:507
Mass (Da):55,459
Checksum:i1E169BE37404520B
GO

Sequence cautioni

The sequence AAA40337 differs from that shown. Reason: Frameshift at position 528.Curated
The sequence AAA40338 differs from that shown. Reason: Frameshift at position 528.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17V → I in AAA40337 (PubMed:3753900).Curated1
Sequence conflicti17V → I in BAA14356 (PubMed:1937024).Curated1
Sequence conflicti17V → I in BAE30084 (PubMed:16141072).Curated1
Sequence conflicti36F → L in AAA40337 (PubMed:3753900).Curated1
Sequence conflicti36F → L in BAA14356 (PubMed:1937024).Curated1
Sequence conflicti140T → A in AAA40337 (PubMed:3753900).Curated1
Sequence conflicti140T → A in BAA14356 (PubMed:1937024).Curated1
Sequence conflicti149 – 151INA → TNT in AAA40337 (PubMed:3753900).Curated3
Sequence conflicti149 – 151INA → TNT in BAA14356 (PubMed:1937024).Curated3
Sequence conflicti151A → T in AAA40338 (PubMed:3753900).Curated1
Sequence conflicti177L → H in BAE31381 (PubMed:16141072).Curated1
Sequence conflicti192V → I in AAA40337 (PubMed:3753900).Curated1
Sequence conflicti192V → I in BAA14356 (PubMed:1937024).Curated1
Sequence conflicti217N → D in BAE39599 (PubMed:16141072).Curated1
Sequence conflicti259K → E in BAE31381 (PubMed:16141072).Curated1
Sequence conflicti296Y → C in AAA40337 (PubMed:3753900).Curated1
Sequence conflicti296Y → C in BAA14356 (PubMed:1937024).Curated1
Sequence conflicti318H → C in AAA40337 (PubMed:3753900).Curated1
Sequence conflicti318H → C in BAA14356 (PubMed:1937024).Curated1
Sequence conflicti326S → T in AAA40337 (PubMed:3753900).Curated1
Sequence conflicti326S → T in BAA14356 (PubMed:1937024).Curated1
Sequence conflicti378R → Q in BAE30084 (PubMed:16141072).Curated1
Sequence conflicti402V → I in BAE30407 (PubMed:16141072).Curated1
Sequence conflicti402V → I in BAE31988 (PubMed:16141072).Curated1
Sequence conflicti405L → S in AAA40337 (PubMed:3753900).Curated1
Sequence conflicti405L → S in BAA14356 (PubMed:1937024).Curated1
Sequence conflicti429S → N in AAA40338 (PubMed:3753900).Curated1
Sequence conflicti439A → V in BAE31381 (PubMed:16141072).Curated1
Sequence conflicti454V → M in BAE39052 (PubMed:16141072).Curated1
Sequence conflicti494K → N in BAE30407 (PubMed:16141072).Curated1
Sequence conflicti537S → C in AAA40337 (PubMed:3753900).Curated1
Sequence conflicti537S → C in BAA14356 (PubMed:1937024).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0247341 – 49Missing in isoform 2. 1 PublicationAdd BLAST49
Alternative sequenceiVSP_02473550G → M in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20130 mRNA. Translation: AAA40337.1. Frameshift.
M12899 mRNA. Translation: AAA40338.1. Frameshift.
D90344 mRNA. Translation: BAA14356.1.
D10606 Genomic DNA. Translation: BAA01461.1.
S46763 Genomic DNA. Translation: AAB23855.1.
AK149611 mRNA. Translation: BAE28989.1.
AK149755 mRNA. Translation: BAE29063.1.
AK151068 mRNA. Translation: BAE30084.1.
AK151445 mRNA. Translation: BAE30407.1.
AK152641 mRNA. Translation: BAE31381.1.
AK153430 mRNA. Translation: BAE31988.1.
AK165665 mRNA. Translation: BAE38327.1.
AK166828 mRNA. Translation: BAE39052.1.
AK166966 mRNA. Translation: BAE39149.1.
AK167529 mRNA. Translation: BAE39599.1.
BC003809 mRNA. Translation: AAH03809.1.
CCDSiCCDS28397.1. [P11983-1]
CCDS70760.1. [P11983-2]
PIRiB24059.
JC1443.
RefSeqiNP_001277641.1. NM_001290712.1. [P11983-2]
NP_038714.2. NM_013686.4. [P11983-1]
UniGeneiMm.229342.

Genome annotation databases

EnsembliENSMUST00000089024; ENSMUSP00000086418; ENSMUSG00000068039. [P11983-2]
ENSMUST00000151287; ENSMUSP00000116108; ENSMUSG00000068039. [P11983-1]
GeneIDi21454.
KEGGimmu:21454.
UCSCiuc008all.2. mouse. [P11983-1]
uc008alm.2. mouse. [P11983-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20130 mRNA. Translation: AAA40337.1. Frameshift.
M12899 mRNA. Translation: AAA40338.1. Frameshift.
D90344 mRNA. Translation: BAA14356.1.
D10606 Genomic DNA. Translation: BAA01461.1.
S46763 Genomic DNA. Translation: AAB23855.1.
AK149611 mRNA. Translation: BAE28989.1.
AK149755 mRNA. Translation: BAE29063.1.
AK151068 mRNA. Translation: BAE30084.1.
AK151445 mRNA. Translation: BAE30407.1.
AK152641 mRNA. Translation: BAE31381.1.
AK153430 mRNA. Translation: BAE31988.1.
AK165665 mRNA. Translation: BAE38327.1.
AK166828 mRNA. Translation: BAE39052.1.
AK166966 mRNA. Translation: BAE39149.1.
AK167529 mRNA. Translation: BAE39599.1.
BC003809 mRNA. Translation: AAH03809.1.
CCDSiCCDS28397.1. [P11983-1]
CCDS70760.1. [P11983-2]
PIRiB24059.
JC1443.
RefSeqiNP_001277641.1. NM_001290712.1. [P11983-2]
NP_038714.2. NM_013686.4. [P11983-1]
UniGeneiMm.229342.

3D structure databases

ProteinModelPortaliP11983.
SMRiP11983.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204044. 14 interactors.
DIPiDIP-32342N.
IntActiP11983. 10 interactors.
MINTiMINT-1862852.
STRINGi10090.ENSMUSP00000116108.

PTM databases

iPTMnetiP11983.
PhosphoSitePlusiP11983.

2D gel databases

REPRODUCTION-2DPAGEIPI00459493.
P11983.

Proteomic databases

EPDiP11983.
MaxQBiP11983.
PaxDbiP11983.
PeptideAtlasiP11983.
PRIDEiP11983.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000089024; ENSMUSP00000086418; ENSMUSG00000068039. [P11983-2]
ENSMUST00000151287; ENSMUSP00000116108; ENSMUSG00000068039. [P11983-1]
GeneIDi21454.
KEGGimmu:21454.
UCSCiuc008all.2. mouse. [P11983-1]
uc008alm.2. mouse. [P11983-2]

Organism-specific databases

CTDi6950.
MGIiMGI:98535. Tcp1.

Phylogenomic databases

eggNOGiKOG0360. Eukaryota.
COG0459. LUCA.
GeneTreeiENSGT00550000074878.
HOVERGENiHBG001052.
InParanoidiP11983.
KOiK09493.
OMAiHYLAQEG.
OrthoDBiEOG091G04HL.
TreeFamiTF106331.

Enzyme and pathway databases

ReactomeiR-MMU-390471. Association of TriC/CCT with target proteins during biosynthesis.
R-MMU-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Miscellaneous databases

ChiTaRSiTcp1. mouse.
PROiP11983.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000068039.
CleanExiMM_TCP1.
ExpressionAtlasiP11983. baseline and differential.
GenevisibleiP11983. MM.

Family and domain databases

CDDicd03335. TCP1_alpha. 1 hit.
Gene3Di1.10.560.10. 2 hits.
3.30.260.10. 2 hits.
3.50.7.10. 1 hit.
InterProiIPR012715. Chap_CCT_alpha.
IPR017998. Chaperone_TCP-1.
IPR002194. Chaperonin_TCP-1_CS.
IPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027413. GROEL-like_equatorial.
IPR027410. TCP-1-like_intermed.
[Graphical view]
PfamiPF00118. Cpn60_TCP1. 1 hit.
[Graphical view]
PRINTSiPR00304. TCOMPLEXTCP1.
SUPFAMiSSF52029. SSF52029. 1 hit.
TIGRFAMsiTIGR02340. chap_CCT_alpha. 1 hit.
PROSITEiPS00750. TCP1_1. 1 hit.
PS00751. TCP1_2. 1 hit.
PS00995. TCP1_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTCPA_MOUSE
AccessioniPrimary (citable) accession number: P11983
Secondary accession number(s): P11984
, Q3TJ96, Q3TKU1, Q3U5T8, Q3U7I8, Q3UAA8, Q3UB80, Q3UE48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 162 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.