P11982 (TY3H_PHASP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine 3-monooxygenase
EC=1.14.16.2

Alternative name(s):
Tyrosine 3-hydroxylase
Short name=TH

Gene names

Name:

TH

Organism

Phasianidae sp. (Quail)

Taxonomic identifier

9006 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the physiology of adrenergic neurons.
Catalytic activity	L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin.
Cofactor	Fe2+ ion.
Enzyme regulation	Phosphorylation leads to an increase in the catalytic activity.
Pathway	Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

Ontologies

Keywords
Biological process	Catecholamine biosynthesis Neurotransmitter biosynthesis
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	aromatic amino acid family metabolic process Inferred from electronic annotation. Source: InterPro catecholamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW neurotransmitter biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: InterPro tyrosine 3-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Tyrosine 3-monooxygenase		PRO_0000205563
Regions
	Compositional bias	51 – 55	5	Poly-Ala
Sites
	Metal binding	324	1	Iron By similarity
	Metal binding	329	1	Iron By similarity
	Metal binding	369	1	Iron By similarity
Amino acid modifications
	Modified residue	19	1	Phosphoserine By similarity
	Modified residue	31	1	Phosphoserine By similarity
	Modified residue	40	1	Phosphoserine; by PKA By similarity

Sequences

	Sequence		Length	Mass (Da)
	P11982 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: AFB363220F70C0A0	FASTA	491	56,066
	10 20 30 40 50 60 MPTPNISTSA AKGFRRAVSE LDSKQAEAIM SPRFIGRRQS LIEDARKERE AAAAATDAAE 70 80 90 100 110 120 STETIVFEEK DGRAMLNLFF MLKGVKTSPL SRALKVFETF EAKIHHLETR LSRKPREGTA 130 140 150 160 170 180 ELEYFVRCEV HSSDLNTFIS SIKRVAEDVR TTKEDKFHWF PRKICELDKC HHLVTKFDPD 190 200 210 220 230 240 LDLDHPGYSD QVYRQRRKSI AEIAFHYKHG DPIPRVEYTA EETATWKEVY STLKSLYPTH 250 260 270 280 290 300 ACKEYLEAFN LLEKFCGYNE NNIPQLEEVS RFLKERTGFQ LRPVRGLLSA RDFLASLAFR 310 320 330 340 350 360 VFQCTQYIRH ASSPMHSPEP DCCHELLGHV PMLADKTFAQ FSQDIGLASL GATDEEIEKL 370 380 390 400 410 420 ATLYWFTVEF GLCRQNGIVK AYGAGLLSSY GELIHSLSDE PEVRDFDPDA AAVQPCQDQP 430 440 450 460 470 480 YQPVYFVSES FSDAKNKLRN YAAHIKRPFS VKYEPYTHSI ELLDSPQTIC HSLESVRDEL 490 HTLINALNVI S

References

[1]	"Cloning of quail tyrosine hydroxylase: amino acid homology with other hydroxylases discloses functional domains." Fauquet M., Grima B., Lamouroux A., Mallet J. J. Neurochem. 50:142-148(1988) [PubMed: 2447231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Adrenal gland.
[2]	"The quail tyrosine hydroxylase gene promoter contains an active cyclic AMP-responsive element." Fauquet M., Boni C. J. Neurochem. 60:274-281(1993) [PubMed: 8093261] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M24778 Genomic DNA. Translation: AAA49514.1. S50878 Genomic DNA. No translation available.
PIR	A28582.
3D structure databases
ProteinModelPortal	P11982.
SMR	P11982. Positions 153-491.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG006841.
Family and domain databases
InterPro	IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR005962. Tyr_3_mOase. IPR019773. Tyrosine_3-monooxygenase-like. IPR021164. Tyrosine_hydroxylase_CS. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF00351. Biopterin_H. 1 hit. PF12549. TOH_N. 2 hits. [Graphical view]
PIRSF	PIRSF000336. TH. 1 hit.
PRINTS	PR00372. FYWHYDRXLASE.
SUPFAM	SSF56534. Aaa_hydroxylase. 1 hit.
TIGRFAMs	TIGR01269. Tyr_3_monoox. 1 hit.
PROSITE	PS00367. BH4_AAA_HYDROXYL_1. 1 hit. PS51410. BH4_AAA_HYDROXYL_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TY3H_PHASP

Accession

Primary (citable) accession number: P11982

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	June 28, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program