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Protein

Regulatory protein SIR4

Gene

SIR4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The proteins SIR1 through SIR4 are required for transcriptional repression of the silent mating type loci, HML and HMR. The proteins SIR2 through SIR4 repress mulitple loci by modulating chromatin structure. Involves the compaction of chromatin fiber into a more condensed form.1 Publication

GO - Molecular functioni

  • double-stranded DNA binding Source: SGD
  • nucleosome binding Source: SGD
  • protein complex scaffold activity Source: SGD

GO - Biological processi

  • chromatin silencing at silent mating-type cassette Source: SGD
  • chromatin silencing at subtelomere Source: SGD
  • chromatin silencing at telomere Source: SGD
  • double-strand break repair via nonhomologous end joining Source: SGD
  • establishment of chromatin silencing at silent mating-type cassette Source: SGD
  • establishment of macromolecular complex localization to telomere Source: SGD
  • negative regulation of chromatin silencing involved in replicative cell aging Source: SGD
  • positive regulation of heterochromatin assembly Source: SGD
  • telomere tethering at nuclear periphery Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29806-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein SIR4
Alternative name(s):
Silent information regulator 4
Gene namesi
Name:SIR4
Synonyms:ASD1, STE9, UTH2
Ordered Locus Names:YDR227W
ORF Names:YD9934.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR227W.
SGDiS000002635. SIR4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000977691 – 1358Regulatory protein SIR4Add BLAST1358

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei692PhosphoserineCombined sources1
Cross-linki1128Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP11978.
PRIDEiP11978.

PTM databases

iPTMnetiP11978.

Interactioni

Subunit structurei

Homodimer. Interacts with MPS3, RIS1, SIR1, SIR2, SIR3, YKU80 and RAP1 C-terminus.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein complex scaffold activity Source: SGD

Protein-protein interaction databases

BioGridi32279. 101 interactors.
DIPiDIP-33N.
IntActiP11978. 31 interactors.
MINTiMINT-622754.
STRINGi4932.YDR227W.

Structurei

Secondary structure

11358
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi808 – 821Combined sources14
Helixi829 – 837Combined sources9
Turni846 – 848Combined sources3
Turni854 – 857Combined sources4
Turni861 – 863Combined sources3
Helixi864 – 866Combined sources3
Helixi869 – 872Combined sources4
Beta strandi879 – 883Combined sources5
Helixi1273 – 1342Combined sources70
Turni1343 – 1345Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NYHX-ray3.10A1198-1358[»]
1PL5X-ray2.50A/S1217-1358[»]
4IAOX-ray2.90C/D737-893[»]
ProteinModelPortaliP11978.
SMRiP11978.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11978.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1271 – 1347Add BLAST77

Keywords - Domaini

Coiled coil

Phylogenomic databases

InParanoidiP11978.
KOiK11123.
OMAiKHASENI.
OrthoDBiEOG092C4Z1J.

Family and domain databases

CDDicd13746. Sir4p-SID_like. 1 hit.
InterProiView protein in InterPro
IPR031556. SIR4_SID.
PfamiView protein in Pfam
PF16991. SIR4_SID. 1 hit.

Sequencei

Sequence statusi: Complete.

P11978-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNDNKTPNR SSTPKFTKKP VTPNDKIPER EEKSNEVKTP KIPLFTFAKS
60 70 80 90 100
KNYSRPSTAI HTSPHQPSDV KPTSHKQLQQ PKSSPLKKNN YNSFPHSNLE
110 120 130 140 150
KISNSKLLSL LRSKTSAGRI ESNNPSHDAS RSLASFEQTA FSRHAQQQTS
160 170 180 190 200
TFNSKPVRTI VPISTSQTNN SFLSGVKSLL SEEKIRDYSK EILGINLANE
210 220 230 240 250
QPVLEKPLKK GSADIGASVI SLTKDKSIRK DTVEEKKEEK LNIGKNFAHS
260 270 280 290 300
DSLSVPKVSA GDSGISPEES KARSPGIAKP NAIQTEVYGI NEESTNERLE
310 320 330 340 350
INQEKPVKLD ENSANSTVAS ALDTNGTSAT TETLTSKKIV PSPKKVAIDQ
360 370 380 390 400
DKITLHDEKT LAPSKHQPIT SEQKMKEDAD LKRMEILKSP HLSKSPADRP
410 420 430 440 450
QGRRNSRNFS TRDEETTKLA FLVEYEGQEN NYNSTSRSTE KKNDMNTSAK
460 470 480 490 500
NKNGENKKIG KRPPEIMSTE AHVNKVTEET TKQIQSVRID GRKVLQKVQG
510 520 530 540 550
ESHIDSRNNT LNVTPSKRPQ LGEIPNPMKK HKPNEGRTPN ISNGTINIQK
560 570 580 590 600
KLEPKEIVRD ILHTKESSNE AKKTIQNPLN KSQNTALPST HKVTQKKDIK
610 620 630 640 650
IGTNDLFQVE SAPKISSEID RENVKSKDEP VSKAVESKSL LNLFSNVLKA
660 670 680 690 700
PFIKSESKPF SSDALSKEKA NFLETIASTE KPENKTDKVS LSQPVSASKH
710 720 730 740 750
EYSDNFPVSL SQPSKKSFAN HTEDEQIEKK KICRGRMNTI ITHPGKMELV
760 770 780 790 800
YVSDSDDSSS DNDSLTDLES LSSGESNEIK VTNDLDTSAE KDQIQAGKWF
810 820 830 840 850
DPVLDWRKSD RELTKNILWR IADKTTYDKE TITDLIEQGI PKHSYLSGNP
860 870 880 890 900
LTSVTNDICS VENYETSSAF FYQQVHKKDR LQYLPLYAVS TFENTNNTEK
910 920 930 940 950
NDVTNKNINI GKHSQEQNSS SAKPSQIPTV SSPLGFEETK LSTTPTKSNR
960 970 980 990 1000
RVSHSDTNSS KPKNTKENLS KSSWRQEWLA NLKLISVSLV DEFPSELSDS
1010 1020 1030 1040 1050
DRQIINEKMQ LLKDIFANNL KSAISNNFRE SDIIILKGEI EDYPMSSEIK
1060 1070 1080 1090 1100
IYYNELQNKP DAKKARFWSF MKTQRFVSNM GFDIQKSCEP VSISTSVKPH
1110 1120 1130 1140 1150
VVEPEHMADA KIMPKDILQI TKKPLMVKNV KPSSPPDVKS LVQLSTMETK
1160 1170 1180 1190 1200
TLPEKKQFDS IFNSNKAKII PGNGKHASEN ISLSFSRPAS YGYFSVGKRV
1210 1220 1230 1240 1250
PIVEDRRVKQ LDDITDSNTT EILTSVDVLG THSQTGTQQS NMYTSTQKTE
1260 1270 1280 1290 1300
LEIDNKDSVT ECSKDMKEDG LSFVDIVLSK AASALDEKEK QLAVANEIIR
1310 1320 1330 1340 1350
SLSDEVMRNE IRITSLQGDL TFTKKCLENA RSQISEKDAK INKLMEKDFQ

VNKEIKPY
Length:1,358
Mass (Da):152,062
Last modified:October 1, 1989 - v1
Checksum:i9C698765964F094E
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti994P → L. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37249 Genomic DNA. Translation: AAA20881.1.
U13239 Genomic DNA. Translation: AAC33144.1.
Z48612 Genomic DNA. Translation: CAA88507.1.
BK006938 Genomic DNA. Translation: DAA12069.1.
PIRiA29360.
RefSeqiNP_010513.1. NM_001180535.1.

Genome annotation databases

EnsemblFungiiYDR227W; YDR227W; YDR227W.
GeneIDi851813.
KEGGisce:YDR227W.

Similar proteinsi

Entry informationi

Entry nameiSIR4_YEAST
AccessioniPrimary (citable) accession number: P11978
Secondary accession number(s): D6VSK9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: September 27, 2017
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names