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P11972

- SST2_YEAST

UniProt

P11972 - SST2_YEAST

Protein

Protein SST2

Gene

SST2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 2 (16 Apr 2002)
      Previous versions | rss
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    Functioni

    Desensitization to alpha-factor pheromone. Is involved in regulating the signaling pathway for responding to mating pheromone.

    GO - Molecular functioni

    1. GTPase activator activity Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Source: SGD
    2. intracellular signal transduction Source: InterPro
    3. positive regulation of GTPase activity Source: GOC
    4. signal transduction Source: SGD
    5. termination of G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Signal transduction inhibitor

    Keywords - Biological processi

    Pheromone response

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32505-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein SST2
    Gene namesi
    Name:SST2
    Ordered Locus Names:YLR452C
    ORF Names:L9324.9
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR452c.
    SGDiS000004444. SST2.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 698698Protein SST2PRO_0000204173Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei252 – 2521Phosphoserine1 Publication
    Modified residuei408 – 4081Phosphoserine1 Publication
    Modified residuei539 – 5391Phosphoserine; by MAPK1 Publication
    Modified residuei587 – 5871Phosphoserine2 Publications

    Post-translational modificationi

    Phosphorylated by FUS3 and KSS1.3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP11972.
    PaxDbiP11972.
    PeptideAtlasiP11972.

    Expressioni

    Inductioni

    By exposure to pheromone.

    Gene expression databases

    GenevestigatoriP11972.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GPA1P085393EBI-18232,EBI-7376
    STE2P0CI394EBI-18232,EBI-18360From a different organism.

    Protein-protein interaction databases

    BioGridi31710. 161 interactions.
    DIPiDIP-2354N.
    IntActiP11972. 11 interactions.
    MINTiMINT-2782980.
    STRINGi4932.YLR452C.

    Structurei

    3D structure databases

    ProteinModelPortaliP11972.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini273 – 35886DEPPROSITE-ProRule annotationAdd
    BLAST
    Domaini420 – 689270RGSPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni10 – 203194Fungal-DRAdd
    BLAST

    Domaini

    The fungal-differentiation regulator (Fungal-DR) domain is only found in fungal regulator of G-protein signaling (RGS) proteins that regulate differentiation pathways. It is required for function By similarity.By similarity

    Sequence similaritiesi

    Contains 1 DEP domain.PROSITE-ProRule annotation
    Contains 1 RGS domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG270876.
    HOGENOMiHOG000248349.
    OMAiKMASERR.
    OrthoDBiEOG793BH8.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR000591. DEP_dom.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00610. DEP. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view]
    SMARTiSM00049. DEP. 2 hits.
    SM00315. RGS. 1 hit.
    [Graphical view]
    SUPFAMiSSF48097. SSF48097. 3 hits.
    PROSITEiPS50186. DEP. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P11972-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVDKNRTLHE LSSKNFSRTP NGLIFTNDLK TVYSIFLICL DLKEKKHSSD    50
    TKSFLLTAFT KHFHFTFTYQ EAIKAMGQLE LKVDMNTTCI NVSYNIKPSL 100
    ARHLLTLFMS SKLLHTPQDR TRGEPKEKVL FQPTPKGVAV LQKYVRDIGL 150
    KTMPDILLSS FNSMKLFTFE RSSVTDSIIH SDYLIHILFI KMMGAKPNVW 200
    SPTNADDPLP CLSSLLEYTN NDDTFTFEKS KPEQGWQAQI GNIDINDLER 250
    VSPLAHRFFT NPDSESHTQY YVSNAGIRLF ENKTFGTSKK IVIKYTFTTK 300
    AIWQWIMDCT DIMHVKEAVS LAALFLKTGL IVPVLLQPSR TDKKKFQISR 350
    SSFFTLSKRG WDLVSWTGCK SNNIRAPNGS TIDLDFTLRG HMTVRDEKKT 400
    LDDSEGFSQD MLISSSNLNK LDYVLTDPGM RYLFRRHLEK ELCVENLDVF 450
    IEIKRFLKKM TILKKLIDSK HCDKKSNTST SKNNIVKTID SALMKQANEC 500
    LEMAYHIYSS YIMIGSPYQL NIHHNLRQNI SDIMLHPHSP LSEHFPTNLY 550
    DPSPASAESA ASSISSTEAD TLGEPPEVSL KPSKNLSNEN CSFKKQGFKH 600
    QLKEYKPAPL TLAETHSPNA SVENSHTIVR YGMDNTQNDT KSVESFPATL 650
    KVLRKLYPLF EIVSNEMYRL MNNDSFQKFT QSDVYKDASA LIEIQEKC 698
    Length:698
    Mass (Da):79,716
    Last modified:April 16, 2002 - v2
    Checksum:i48CF5BB6E456C11C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti616 – 6161H → D in AAA35104. (PubMed:2830483)Curated
    Sequence conflicti644 – 6441E → D in AAA35104. (PubMed:2830483)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M18105 Genomic DNA. Translation: AAA35104.1.
    U22382 Genomic DNA. Translation: AAB67534.1.
    BK006945 Genomic DNA. Translation: DAA09752.1.
    PIRiS55974.
    RefSeqiNP_013557.1. NM_001182340.1.

    Genome annotation databases

    EnsemblFungiiYLR452C; YLR452C; YLR452C.
    GeneIDi851173.
    KEGGisce:YLR452C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M18105 Genomic DNA. Translation: AAA35104.1 .
    U22382 Genomic DNA. Translation: AAB67534.1 .
    BK006945 Genomic DNA. Translation: DAA09752.1 .
    PIRi S55974.
    RefSeqi NP_013557.1. NM_001182340.1.

    3D structure databases

    ProteinModelPortali P11972.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31710. 161 interactions.
    DIPi DIP-2354N.
    IntActi P11972. 11 interactions.
    MINTi MINT-2782980.
    STRINGi 4932.YLR452C.

    Proteomic databases

    MaxQBi P11972.
    PaxDbi P11972.
    PeptideAtlasi P11972.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR452C ; YLR452C ; YLR452C .
    GeneIDi 851173.
    KEGGi sce:YLR452C.

    Organism-specific databases

    CYGDi YLR452c.
    SGDi S000004444. SST2.

    Phylogenomic databases

    eggNOGi NOG270876.
    HOGENOMi HOG000248349.
    OMAi KMASERR.
    OrthoDBi EOG793BH8.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32505-MONOMER.

    Miscellaneous databases

    NextBioi 967987.

    Gene expression databases

    Genevestigatori P11972.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR000591. DEP_dom.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00610. DEP. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view ]
    SMARTi SM00049. DEP. 2 hits.
    SM00315. RGS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48097. SSF48097. 3 hits.
    PROSITEi PS50186. DEP. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Pheromonal regulation and sequence of the Saccharomyces cerevisiae SST2 gene: a model for desensitization to pheromone."
      Dietzel C., Kurjan J.
      Mol. Cell. Biol. 7:4169-4177(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Feedback phosphorylation of an RGS protein by MAP kinase in yeast."
      Garrison T.R., Zhang Y., Pausch M., Apanovitch D., Aebersold R., Dohlman H.G.
      J. Biol. Chem. 274:36387-36391(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-539.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-252 AND SER-587, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-408 AND SER-587, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSST2_YEAST
    AccessioniPrimary (citable) accession number: P11972
    Secondary accession number(s): D6VZ86, Q06207
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: April 16, 2002
    Last modified: October 1, 2014
    This is version 135 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5980 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3