P11972 (SST2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein SST2

Gene names

Name:	SST2
Ordered Locus Names:	YLR452C
ORF Names:	L9324.9

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	698 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Desensitization to alpha-factor pheromone. Is involved in regulating the signaling pathway for responding to mating pheromone.
Induction	By exposure to pheromone.
Domain	The fungal-differentiation regulator (Fungal-DR) domain is only found in fungal regulator of G-protein signaling (RGS) proteins that regulate differentiation pathways. It is required for function By similarity.
Post-translational modification	Phosphorylated by FUS3 and KSS1. Ref.4
Miscellaneous	Present with 5980 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 DEP domain. Contains 1 RGS domain.

Ontologies

Keywords
Biological process	Pheromone response
Molecular function	Signal transduction inhibitor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Inferred from mutant phenotype Ref.1. Source: SGD intracellular signal transduction Inferred from electronic annotation. Source: InterPro signal transduction Inferred from mutant phenotype PubMed 9537998. Source: SGD termination of G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	plasma membrane Inferred from direct assay PubMed 8756677. Source: SGD
Molecular_function	GTPase activator activity Inferred from direct assay PubMed 9537998. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GPA1	P08539	3	EBI-18232,EBI-7376
STE2	P0CI39	4	EBI-18232,EBI-18360	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 698	698	Protein SST2		PRO_0000204173
Regions
	Domain	273 – 358	86	DEP
	Domain	420 – 689	270	RGS
	Region	10 – 203	194	Fungal-DR
Amino acid modifications
	Modified residue	252	1	Phosphoserine Ref.7
	Modified residue	380	1	Phosphoserine Ref.6 Ref.9
	Modified residue	408	1	Phosphoserine Ref.9
	Modified residue	539	1	Phosphoserine; by MAPK Ref.4 Ref.9
	Modified residue	587	1	Phosphoserine Ref.6 Ref.7 Ref.8 Ref.9
	Modified residue	592	1	Phosphoserine Ref.6 Ref.9
	Modified residue	617	1	Phosphoserine Ref.9
Experimental info
	Sequence conflict	616	1	H → D in AAA35104. Ref.1
	Sequence conflict	644	1	E → D in AAA35104. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P11972 [UniParc]. Last modified April 16, 2002. Version 2. Checksum: 48CF5BB6E456C11C	FASTA	698	79,716
	10 20 30 40 50 60 MVDKNRTLHE LSSKNFSRTP NGLIFTNDLK TVYSIFLICL DLKEKKHSSD TKSFLLTAFT 70 80 90 100 110 120 KHFHFTFTYQ EAIKAMGQLE LKVDMNTTCI NVSYNIKPSL ARHLLTLFMS SKLLHTPQDR 130 140 150 160 170 180 TRGEPKEKVL FQPTPKGVAV LQKYVRDIGL KTMPDILLSS FNSMKLFTFE RSSVTDSIIH 190 200 210 220 230 240 SDYLIHILFI KMMGAKPNVW SPTNADDPLP CLSSLLEYTN NDDTFTFEKS KPEQGWQAQI 250 260 270 280 290 300 GNIDINDLER VSPLAHRFFT NPDSESHTQY YVSNAGIRLF ENKTFGTSKK IVIKYTFTTK 310 320 330 340 350 360 AIWQWIMDCT DIMHVKEAVS LAALFLKTGL IVPVLLQPSR TDKKKFQISR SSFFTLSKRG 370 380 390 400 410 420 WDLVSWTGCK SNNIRAPNGS TIDLDFTLRG HMTVRDEKKT LDDSEGFSQD MLISSSNLNK 430 440 450 460 470 480 LDYVLTDPGM RYLFRRHLEK ELCVENLDVF IEIKRFLKKM TILKKLIDSK HCDKKSNTST 490 500 510 520 530 540 SKNNIVKTID SALMKQANEC LEMAYHIYSS YIMIGSPYQL NIHHNLRQNI SDIMLHPHSP 550 560 570 580 590 600 LSEHFPTNLY DPSPASAESA ASSISSTEAD TLGEPPEVSL KPSKNLSNEN CSFKKQGFKH 610 620 630 640 650 660 QLKEYKPAPL TLAETHSPNA SVENSHTIVR YGMDNTQNDT KSVESFPATL KVLRKLYPLF 670 680 690 EIVSNEMYRL MNNDSFQKFT QSDVYKDASA LIEIQEKC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pheromonal regulation and sequence of the Saccharomyces cerevisiae SST2 gene: a model for desensitization to pheromone." Dietzel C., Kurjan J. Mol. Cell. Biol. 7:4169-4177(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Feedback phosphorylation of an RGS protein by MAP kinase in yeast." Garrison T.R., Zhang Y., Pausch M., Apanovitch D., Aebersold R., Dohlman H.G. J. Biol. Chem. 274:36387-36391(1999) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-539.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-380; SER-587 AND SER-592, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-252 AND SER-587, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-587, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-380; SER-408; SER-539; SER-587; SER-592 AND SER-617, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M18105 Genomic DNA. Translation: AAA35104.1. U22382 Genomic DNA. Translation: AAB67534.1. BK006945 Genomic DNA. Translation: DAA09752.1.
PIR	S55974.
RefSeq	NP_013557.1. NM_001182340.1.
3D structure databases
ProteinModelPortal	P11972.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2354N.
IntAct	P11972. 11 interactions.
MINT	MINT-2782980.
STRING	4932.YLR452C.
Proteomic databases
PaxDb	P11972.
PeptideAtlas	P11972.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR452C; YLR452C; YLR452C.
GeneID	851173.
KEGG	sce:YLR452C.
Organism-specific databases
CYGD	YLR452c.
SGD	S000004444. SST2.
Phylogenomic databases
eggNOG	NOG270876.
HOGENOM	HOG000248349.
OMA	DPGMRYL.
OrthoDB	EOG45QMNK.
Gene expression databases
Genevestigator	P11972.
GermOnline	YLR452C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR000591. DEP_dom. IPR000342. Regulat_G_prot_signal. IPR016137. Regulat_G_prot_signal_superfam. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00610. DEP. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
SMART	SM00049. DEP. 2 hits. SM00315. RGS. 1 hit. [Graphical view]
SUPFAM	SSF48097. Regulat_G_prot_signal_superfam. 1 hit.
PROSITE	PS50186. DEP. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967987.

Entry information

Entry name

SST2_YEAST

Accession

Primary (citable) accession number: P11972
Secondary accession number(s): D6VZ86, Q06207

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	April 16, 2002
Last modified:	May 1, 2013
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program