Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial

Gene

Bckdha

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).

Catalytic activityi

3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi202 – 2021PotassiumBy similarity
Metal bindingi207 – 2071PotassiumBy similarity
Metal bindingi208 – 2081PotassiumBy similarity

GO - Molecular functioni

GO - Biological processi

  • branched-chain amino acid catabolic process Source: HGNC
  • response to cAMP Source: RGD
  • response to glucocorticoid Source: RGD
  • response to nutrient Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, Potassium, Thiamine pyrophosphate

Enzyme and pathway databases

SABIO-RKP11960.

Names & Taxonomyi

Protein namesi
Recommended name:
2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial (EC:1.2.4.4)
Alternative name(s):
Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain
Short name:
BCKDE1A
Short name:
BCKDH E1-alpha
Gene namesi
Name:Bckdha
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2196. Bckdha.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial alpha-ketoglutarate dehydrogenase complex Source: HGNC
  • mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei‹1 – 41›41MitochondrionBy similarityAdd
BLAST
Chaini42 – 4414002-oxoisovalerate dehydrogenase subunit alpha, mitochondrialPRO_0000020468Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei333 – 3331PhosphoserineCombined sources
Modified residuei334 – 3341PhosphothreonineBy similarity
Modified residuei335 – 3351PhosphoserineBy similarity
Modified residuei343 – 3431PhosphoserineCombined sources
Modified residuei352 – 3521N6-acetyllysine; alternateBy similarity
Modified residuei352 – 3521N6-succinyllysine; alternateBy similarity
Modified residuei376 – 3761N6-succinyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP11960.
PRIDEiP11960.

PTM databases

iPTMnetiP11960.
PhosphoSiteiP11960.

Expressioni

Gene expression databases

BgeeiENSRNOG00000020607.

Interactioni

Subunit structurei

Heterotetramer of alpha and beta chains.By similarity

GO - Molecular functioni

  • protein complex binding Source: RGD

Protein-protein interaction databases

IntActiP11960. 1 interaction.
STRINGi10116.ENSRNOP00000027995.

Structurei

3D structure databases

ProteinModelPortaliP11960.
SMRiP11960. Positions 47-441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni153 – 1553Thiamine pyrophosphate bindingBy similarity

Sequence similaritiesi

Belongs to the BCKDHA family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1182. Eukaryota.
COG1071. LUCA.
HOVERGENiHBG002459.
InParanoidiP11960.
KOiK00166.
PhylomeDBiP11960.

Family and domain databases

Gene3Di3.40.50.970. 1 hit.
InterProiIPR001017. DH_E1.
IPR029061. THDP-binding.
[Graphical view]
PfamiPF00676. E1_dh. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11960-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
SAAKIWRPSR GLRQAALLLL GRPGARGLAR FHPSRQQQQQ FPSLDDKPQF
60 70 80 90 100
PGASAEFVDK LEFIQPNVIS GIPIYRVMDR QGQIINPSED PHLPQEEVLK
110 120 130 140 150
LYRSMTLLNT MDRILYESQR QGRISFYMTN YGEEGTHVGS AAALERTDLV
160 170 180 190 200
FGQYREAGVL MYRDYPLELF MAQCYGNVSD PGKGRQMPVH YGCKERHFVT
210 220 230 240 250
ISSPLATQIP QAVGAAYAAK RANANQIVIC YFGEGAASEG DAHAGFNFAA
260 270 280 290 300
TLECPIIFFC RNNGYAISTP TSEQYRGDGI AARGPGYGIM SIRVDGNDVF
310 320 330 340 350
AVYNATKEAR RRAVAENQPF LIEAMTYRIG HHSTSDDSSA YRSVDEVNYW
360 370 380 390 400
DKQDHPISRL RQYLLNQGWW DEEQEKAWRK QSRKKVMEAF EQAERKLKPN
410 420 430 440
PSLLFSDVYQ EMPAQLRRQQ ESLARHLQTY GEHYPLDHFD K
Length:441
Mass (Da):50,164
Last modified:October 1, 1989 - v1
Checksum:iFFD7709A94094C8E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02827 mRNA. Translation: AAA40811.1.
PIRiA29468. DERTXA.
RefSeqiNP_036914.1. NM_012782.1.
UniGeneiRn.49145.

Genome annotation databases

GeneIDi25244.
KEGGirno:25244.
UCSCiRGD:2196. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02827 mRNA. Translation: AAA40811.1.
PIRiA29468. DERTXA.
RefSeqiNP_036914.1. NM_012782.1.
UniGeneiRn.49145.

3D structure databases

ProteinModelPortaliP11960.
SMRiP11960. Positions 47-441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP11960. 1 interaction.
STRINGi10116.ENSRNOP00000027995.

PTM databases

iPTMnetiP11960.
PhosphoSiteiP11960.

Proteomic databases

PaxDbiP11960.
PRIDEiP11960.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25244.
KEGGirno:25244.
UCSCiRGD:2196. rat.

Organism-specific databases

CTDi593.
RGDi2196. Bckdha.

Phylogenomic databases

eggNOGiKOG1182. Eukaryota.
COG1071. LUCA.
HOVERGENiHBG002459.
InParanoidiP11960.
KOiK00166.
PhylomeDBiP11960.

Enzyme and pathway databases

SABIO-RKP11960.

Gene expression databases

BgeeiENSRNOG00000020607.

Family and domain databases

Gene3Di3.40.50.970. 1 hit.
InterProiIPR001017. DH_E1.
IPR029061. THDP-binding.
[Graphical view]
PfamiPF00676. E1_dh. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiODBA_RAT
AccessioniPrimary (citable) accession number: P11960
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: September 7, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Bound potassium ions stabilize the protein structure.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.