Reviewed, UniProtKB/Swiss-Prot P11957 (MT1A_RABIT)

Names and origin

Protein names	Recommended name:     Metallothionein-1A       Short name=MT-1A Alternative name(s):     Metallothionein-IA       Short name=MT-IA
Organism	Oryctolagus cuniculus (Rabbit)
Taxonomic identifier	9986 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	61 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Subunit structure	Monomer.
Domain	Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Ligand	Cadmium Copper Metal-binding Metal-thiolate cluster Zinc
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	cadmium ion binding Inferred from electronic annotation. Source: UniProtKB-KW copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 61	61	Metallothionein-1A		PRO_0000197215
Regions
	Region	1 – 29	29	Beta
	Region	30 – 61	32	Alpha
Sites
	Metal binding	5	1	Cluster B
	Metal binding	7	1	Cluster B
	Metal binding	13	1	Cluster B
	Metal binding	15	1	Cluster B
	Metal binding	19	1	Cluster B
	Metal binding	21	1	Cluster B
	Metal binding	24	1	Cluster B
	Metal binding	26	1	Cluster B
	Metal binding	29	1	Cluster B
	Metal binding	33	1	Cluster A
	Metal binding	34	1	Cluster A
	Metal binding	36	1	Cluster A
	Metal binding	37	1	Cluster A
	Metal binding	41	1	Cluster A
	Metal binding	44	1	Cluster A
	Metal binding	48	1	Cluster A
	Metal binding	50	1	Cluster A
	Metal binding	57	1	Cluster A
	Metal binding	59	1	Cluster A
	Metal binding	60	1	Cluster A
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine

Sequences

	Sequence		Length	Mass (Da)
	P11957-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: F90A73BFFF6E0DD1	FASTA	61	6,103
	10 20 30 40 50 60 MDPNCSCATG NSCTCASSCK CKECKCTSCK KSCCSCCPAG CTKCAQGCIC KGASDKCSCC A

References

[1]	"Cloning, nucleotide sequence and characterization of a New Zealand rabbit metallothionein-I gene." Tam Y.C., Chopra A., Hassan M., Thirion J.-P. Biochem. Biophys. Res. Commun. 153:209-216(1988) [PubMed: 2837200] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: New Zealand white.
[2]	"Primary structures of seven metallothioneins from rabbit tissue." Hunziker P.E., Kaur P., Wan M., Kaenzig A. Biochem. J. 306:265-270(1995) [PubMed: 7864820] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: New Zealand white. Tissue: Kidney and Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M20015 Genomic DNA. Translation: AAA31147.1.
PIR	A27652.
3D structure databases
SMR	P11957. Positions 1-61.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P11957.
Family and domain databases
InterPro	IPR017854. Metallothionein_sfam. IPR003019. Metallothionein_sfam_euk. IPR000006. Metallothionein_vert. IPR018064. Metallothionein_vert_metal_BS. [Graphical view]
Gene3D	G3DSA:4.10.10.10. Metallothionein_vert. 1 hit.
PANTHER	PTHR23299. Metallothionein_vert. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00860. MTVERTEBRATE.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MT1A_RABIT

Accession

Primary (citable) accession number: P11957

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	November 24, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)