Reviewed, UniProtKB/Swiss-Prot P11955 (CHI1_HORVU)

Names and origin

Protein names	Recommended name:     26 kDa endochitinase 1     EC=3.2.1.14
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Induction	By ethylene.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 318	299	26 kDa endochitinase 1		PRO_0000005296
Regions
	Domain	20 – 59	40	Chitin-binding type-1
Amino acid modifications
	Disulfide bond	22 ↔ 37		By similarity
	Disulfide bond	31 ↔ 43		By similarity
	Disulfide bond	36 ↔ 49		By similarity
	Disulfide bond	53 ↔ 57		By similarity
	Disulfide bond	98 ↔ 160		By similarity
	Disulfide bond	172 ↔ 180		By similarity
	Disulfide bond	279 ↔ 311		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P11955-1 [UniParc]. Last modified November 1, 1997. Version 4. Checksum: 42D62B2FE8041954	FASTA	318	33,402
	10 20 30 40 50 60 MRAFVLFAVV AMAATMAVAE QCGSQAGGAT CPNCLCCSRF GWCGSTPYCG DGCQSQCSGC 70 80 90 100 110 120 GGGSTPVTPT PSGGGGVSSI VSRALFDRML LHRNDGACQA KGFYTYDAFV AAASAFRGFG 130 140 150 160 170 180 TTGGTDTRKR EVAAFLAQTS HETTGGWATA PDGAFAWGYC FKQERGATSN YCTPSAQWPC 190 200 210 220 230 240 APGKSYYGRG PIQLSHNYNY GPAGRAIGVD LLRNPDLVAT DPTVSFKTAM WFWMTAQAPK 250 260 270 280 290 300 PSSHAVITGQ WSPSGTDRAA GRVPGFGVIT NIVNGGIECG HGQDSRVADR IGFYKRYCDI 310 LGVGYGNNLD CYSQRPFA

References

[1]	Ignatius S.M.J., Huang J., Muthukrishnan S. Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. NK 1558. Tissue: Leaf.
[2]	"Identification of an endochitinase cDNA clone from barley aleurone cells." Swegle M., Huang J.-K., Lee G., Muthukrishnan S. Plant Mol. Biol. 12:403-412(1989) Cited for: NUCLEOTIDE SEQUENCE OF 141-318. Strain: cv. Himalaya.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U02287 Unassigned DNA. Translation: AAA18586.1. X15349 mRNA. Translation: CAA33407.1.
PIR	S04131. T04403.
3D structure databases
HSSP	HSSP built from PDB template 1CNS based on UniProtKB P23951.
SMR	P11955. Positions 20-317, 77-318.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Organism-specific databases
Gramene	P11955.
Enzyme and pathway databases
BRENDA	3.2.1.14. 283.
Family and domain databases
InterPro	IPR018371. Chitin-binding_1_CS. IPR001002. Chitin_bd_1. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view]
Gene3D	G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_binding_1. 1 hit. PD354900. Glyco_hydro_19. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI1_HORVU

Accession

Primary (citable) accession number: P11955

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 78 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)