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Protein

26 kDa endochitinase 1

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Defense against chitin containing fungal pathogens.

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Enzyme and pathway databases

BRENDAi3.2.1.14. 2687.

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
26 kDa endochitinase 1 (EC:3.2.1.14)
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Organism-specific databases

GrameneiP11955.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 31829926 kDa endochitinase 1PRO_0000005296Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi22 ↔ 37PROSITE-ProRule annotation
Disulfide bondi31 ↔ 43PROSITE-ProRule annotation
Disulfide bondi36 ↔ 49PROSITE-ProRule annotation
Disulfide bondi53 ↔ 57PROSITE-ProRule annotation
Disulfide bondi98 ↔ 160PROSITE-ProRule annotation
Disulfide bondi172 ↔ 180PROSITE-ProRule annotation
Disulfide bondi279 ↔ 311PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

By ethylene.

Structurei

3D structure databases

ProteinModelPortaliP11955.
SMRiP11955. Positions 20-318.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 5940Chitin-binding type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 chitin-binding type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11955-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAFVLFAVV AMAATMAVAE QCGSQAGGAT CPNCLCCSRF GWCGSTPYCG
60 70 80 90 100
DGCQSQCSGC GGGSTPVTPT PSGGGGVSSI VSRALFDRML LHRNDGACQA
110 120 130 140 150
KGFYTYDAFV AAASAFRGFG TTGGTDTRKR EVAAFLAQTS HETTGGWATA
160 170 180 190 200
PDGAFAWGYC FKQERGATSN YCTPSAQWPC APGKSYYGRG PIQLSHNYNY
210 220 230 240 250
GPAGRAIGVD LLRNPDLVAT DPTVSFKTAM WFWMTAQAPK PSSHAVITGQ
260 270 280 290 300
WSPSGTDRAA GRVPGFGVIT NIVNGGIECG HGQDSRVADR IGFYKRYCDI
310
LGVGYGNNLD CYSQRPFA
Length:318
Mass (Da):33,402
Last modified:November 1, 1997 - v4
Checksum:i42D62B2FE8041954
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02287 Unassigned DNA. Translation: AAA18586.1.
X15349 mRNA. Translation: CAA33407.1.
PIRiS04131.
T04403.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02287 Unassigned DNA. Translation: AAA18586.1.
X15349 mRNA. Translation: CAA33407.1.
PIRiS04131.
T04403.

3D structure databases

ProteinModelPortaliP11955.
SMRiP11955. Positions 20-318.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GrameneiP11955.

Enzyme and pathway databases

BRENDAi3.2.1.14. 2687.

Family and domain databases

Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Ignatius S.M.J., Huang J., Muthukrishnan S.
    Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: cv. NK 1558.
    Tissue: Leaf.
  2. "Identification of an endochitinase cDNA clone from barley aleurone cells."
    Swegle M., Huang J.-K., Lee G., Muthukrishnan S.
    Plant Mol. Biol. 12:403-412(1989)
    Cited for: NUCLEOTIDE SEQUENCE OF 141-318.
    Strain: cv. Himalaya.

Entry informationi

Entry nameiCHI1_HORVU
AccessioniPrimary (citable) accession number: P11955
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: November 1, 1997
Last modified: July 22, 2015
This is version 95 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.