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Protein

T-cell surface glycoprotein CD3 gamma chain

Gene

Cd3g

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G (PubMed:25920998).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionReceptor
Biological processAdaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-MMU-202424. Downstream TCR signaling.
R-MMU-202427. Phosphorylation of CD3 and TCR zeta chains.
R-MMU-202430. Translocation of ZAP-70 to Immunological synapse.
R-MMU-202433. Generation of second messenger molecules.
R-MMU-2029481. FCGR activation.
R-MMU-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-MMU-2029485. Role of phospholipids in phagocytosis.
R-MMU-389948. PD-1 signaling.
R-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD3 gamma chain
Alternative name(s):
T-cell receptor T3 gamma chain
CD_antigen: CD3g
Gene namesi
Name:Cd3g
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:88333. Cd3g.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 116ExtracellularSequence analysisAdd BLAST94
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Topological domaini138 – 182CytoplasmicSequence analysisAdd BLAST45

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

The absence of CD3G results in a severe reduction in the level of the TCR-CD3 at the cell surface of thymocytes and peripheral T cells. The development of both the TCRalphabeta and TCRgammadelta lineages are affected by the absence of CD3G.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Add BLAST22
ChainiPRO_000001461723 – 182T-cell surface glycoprotein CD3 gamma chainAdd BLAST160

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi46 ↔ 87By similarity
Glycosylationi66N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei145PhosphoserineBy similarity1
Modified residuei148PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. Phosphorylated also by PKC; leading to the TCR complex down-regulation.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP11942.
PaxDbiP11942.
PRIDEiP11942.

PTM databases

iPTMnetiP11942.
PhosphoSitePlusiP11942.

Expressioni

Gene expression databases

BgeeiENSMUSG00000002033.
CleanExiMM_CD3G.
ExpressionAtlasiP11942. baseline and differential.
GenevisibleiP11942. MM.

Interactioni

Subunit structurei

The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta.By similarity

GO - Molecular functioni

Protein-protein interaction databases

CORUMiP11942.
ELMiP11942.
IntActiP11942. 1 interactor.
STRINGi10090.ENSMUSP00000002101.

Structurei

Secondary structure

1182
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi37 – 39Combined sources3
Beta strandi41 – 45Combined sources5
Beta strandi47 – 49Combined sources3
Beta strandi53 – 57Combined sources5
Beta strandi73 – 76Combined sources4
Beta strandi82 – 91Combined sources10
Beta strandi93 – 100Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JBJNMR-A23-103[»]
ProteinModelPortaliP11942.
SMRiP11942.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11942.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 94Ig-likeAdd BLAST58
Domaini149 – 177ITAMPROSITE-ProRule annotationAdd BLAST29

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IWAX. Eukaryota.
ENOG410Y2TD. LUCA.
GeneTreeiENSGT00510000046930.
HOGENOMiHOG000015287.
HOVERGENiHBG005278.
InParanoidiP11942.
KOiK06452.
OMAiDRENDQY.
OrthoDBiEOG091G0MXQ.
PhylomeDBiP11942.
TreeFamiTF335892.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR015484. CD3_esu/gsu/dsu.
IPR036179. Ig-like_dom_sf.
IPR013783. Ig-like_fold.
IPR032052. Ig_4.
IPR003598. Ig_sub2.
IPR003110. Phos_immunorcpt_sig_ITAM.
PANTHERiPTHR10570. PTHR10570. 1 hit.
PfamiView protein in Pfam
PF16680. Ig_4. 1 hit.
PF02189. ITAM. 1 hit.
SMARTiView protein in SMART
SM00408. IGc2. 1 hit.
SM00077. ITAM. 1 hit.
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiView protein in PROSITE
PS51055. ITAM_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11942-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQRKGLAGL FLVISLLQGT VAQTNKAKNL VQVDGSRGDG SVLLTCGLTD
60 70 80 90 100
KTIKWLKDGS IISPLNATKN TWNLGNNAKD PRGTYQCQGA KETSNPLQVY
110 120 130 140 150
YRMCENCIEL NIGTISGFIF AEVISIFFLA LGVYLIAGQD GVRQSRASDK
160 170 180
QTLLQNEQLY QPLKDREYDQ YSHLQGNQLR KK
Length:182
Mass (Da):20,234
Last modified:October 1, 1989 - v1
Checksum:i7F9D8D44A5C360D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00635 mRNA. Translation: CAA68667.1.
M58149 mRNA. Translation: AAA63289.1. Sequence problems.
M18227
, M18222, M18223, M18224, M18225, M18226 Genomic DNA. Translation: AAA37400.1.
BC027528 mRNA. Translation: AAH27528.1.
CCDSiCCDS23123.1.
PIRiA39952. A28508.
RefSeqiNP_033980.1. NM_009850.2.
UniGeneiMm.335106.

Genome annotation databases

EnsembliENSMUST00000002101; ENSMUSP00000002101; ENSMUSG00000002033.
GeneIDi12502.
KEGGimmu:12502.
UCSCiuc009pex.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiCD3G_MOUSE
AccessioniPrimary (citable) accession number: P11942
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 22, 2017
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references