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Protein

Lysozyme C II

Gene
N/A
Organism
Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has antibacterial activity against the Gram positive bacterium P.citreus. Has no antibacterial activity against the Gram negative bacteria E.coli and Y.ruckeri. Does not have hemolytic activity against trout erythrocytes.PROSITE-ProRule annotation1 Publication

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.1 Publication

pH dependencei

Optimum pH is 4.5-5.5. No activity above pH 8.8.1 Publication

Temperature dependencei

Optimum temperature is 33-49 degrees Celsius. Retains 25% of its maximal activity after heating 10 minutes at 80 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei501
Active sitei671

GO - Molecular functioni

  • lysozyme activity Source: AgBase

GO - Biological processi

Keywordsi

Molecular functionAntibiotic, Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH22. Glycoside Hydrolase Family 22.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysozyme C II (EC:3.2.1.17)
Alternative name(s):
1,4-beta-N-acetylmuramidase C
Lysozyme type II
OrganismiOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Taxonomic identifieri8022 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 152 PublicationsAdd BLAST15
ChainiPRO_000001850116 – 144Lysozyme C IIAdd BLAST129

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi21 ↔ 142
Disulfide bondi45 ↔ 130
Disulfide bondi79 ↔ 95
Disulfide bondi91 ↔ 109

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP11941.

Structurei

Secondary structure

1144
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi20 – 29Combined sources10
Helixi40 – 51Combined sources12
Beta strandi58 – 60Combined sources3
Beta strandi66 – 68Combined sources3
Turni69 – 72Combined sources4
Turni75 – 77Combined sources3
Helixi95 – 98Combined sources4
Beta strandi99 – 102Combined sources4
Helixi104 – 113Combined sources10
Helixi119 – 122Combined sources4
Helixi124 – 129Combined sources6
Turni130 – 132Combined sources3
Helixi136 – 138Combined sources3
Turni139 – 141Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BB6X-ray2.00A16-144[»]
1BB7X-ray2.00A16-144[»]
1LMCX-ray2.00A16-144[»]
1LMNX-ray1.80A16-144[»]
1LMOX-ray1.80A16-144[»]
1LMPX-ray2.00A16-144[»]
1LMQX-ray1.60A16-144[»]
ProteinModelPortaliP11941.
SMRiP11941.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11941.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 22 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG052297.

Family and domain databases

CDDicd00119. LYZ1. 1 hit.
InterProiView protein in InterPro
IPR001916. Glyco_hydro_22.
IPR019799. Glyco_hydro_22_CS.
IPR000974. Glyco_hydro_22_lys.
IPR023346. Lysozyme-like_dom_sf.
IPR030056. Lysozyme_C.
PANTHERiPTHR11407:SF28. PTHR11407:SF28. 1 hit.
PfamiView protein in Pfam
PF00062. Lys. 1 hit.
PRINTSiPR00137. LYSOZYME.
PR00135. LYZLACT.
SMARTiView protein in SMART
SM00263. LYZ1. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiView protein in PROSITE
PS00128. LACTALBUMIN_LYSOZYME_1. 1 hit.
PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11941-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAVVVLLLV AVASAKVYDR CELARALKAS GMDGYAGNSL PNWVCLSKWE
60 70 80 90 100
SSYNTQATNR NTDGSTDYGI FQINSRYWCD DGRTPGAKNV CGIRCSQLLT
110 120 130 140
ADLTVAIRCA KRVVLDPNGI GAWVAWRLHC QNQDLRSYVA GCGV
Length:144
Mass (Da):15,737
Last modified:April 1, 1993 - v2
Checksum:iBF945ADCDCC2D668
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94R → H in AAG34564 (Ref. 3) Curated1
Sequence conflicti101A → D in AAG34564 (Ref. 3) Curated1

Mass spectrometryi

Molecular mass is 14252 Da from positions 16 - 144. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59491 mRNA. Translation: CAA42084.1.
AF322106 Genomic DNA. Translation: AAG45933.1.
AF452171 Genomic DNA. Translation: AAL48290.1.
AF321519 mRNA. Translation: AAG34564.1.
PIRiI51047.
RefSeqiNP_001118188.1. NM_001124716.1.
UniGeneiOmy.11916.

Genome annotation databases

GeneIDi100136768.

Similar proteinsi

Entry informationi

Entry nameiLYSC2_ONCMY
AccessioniPrimary (citable) accession number: P11941
Secondary accession number(s): P83333, Q9DDK3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: April 1, 1993
Last modified: November 22, 2017
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families