UniProtKB - P11938 (RAP1_YEAST)
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Protein
DNA-binding protein RAP1
Gene
RAP1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Essential regulatory protein in yeast whose DNA-binding sites are found at three types of chromosomal elements: promoters, silencers, and telomeres. RAP1 is also involved in the regulation of telomere structure, where its binding sites are found within the terminal poly[C1-3A] sequences. The opposite regulatory functions of RAP1 are not intrinsic to its binding sites but, instead, result from interactions with different factors at promoters and silencers. RAP1 associates with SIR3 and SIR4 proteins to form a DNA-binding complex that initiates the repression at the HM loci and telomeres. May also target the binding of RIF1 and RIF2 to silencers and telomeres. Forms with GCR1 a transcriptional activation complex that is required for expression of glycolytic and ribosomal gene.
Miscellaneous
Present with 4390 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 388 – 411 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- DNA binding, bending Source: SGD
- double-stranded telomeric DNA binding Source: CAFA
- G-quadruplex DNA binding Source: SGD
- nucleosomal DNA binding Source: SGD
- proximal promoter sequence-specific DNA binding Source: SGD
- RNA polymerase II transcription factor activity, TBP-class protein binding Source: SGD
- telomeric DNA binding Source: SGD
- TFIID-class transcription factor binding Source: SGD
- transcription factor activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: SGD
- transcription factor activity, RNA polymerase II transcription factor binding Source: SGD
GO - Biological processi
- chromatin silencing at silent mating-type cassette Source: SGD
- chromatin silencing at telomere Source: SGD
- establishment of chromatin silencing at telomere Source: SGD
- establishment of protein localization to chromatin Source: SGD
- establishment of protein localization to telomere Source: SGD
- G-quadruplex DNA formation Source: CACAO
- negative regulation of chromatin silencing Source: SGD
- negative regulation of transcription by RNA polymerase II Source: SGD
- positive regulation of transcription by RNA polymerase II Source: SGD
- protection from non-homologous end joining at telomere Source: SGD
- regulation of glycolytic process by positive regulation of transcription from RNA polymerase II promoter Source: SGD
- telomere maintenance Source: SGD
- telomere maintenance via telomere lengthening Source: SGD
- transcription, DNA-templated Source: UniProtKB-KW
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | YEAST:G3O-33222-MONOMER. |
Names & Taxonomyi
Protein namesi | Recommended name: DNA-binding protein RAP1Alternative name(s): Repressor/activator site-binding protein SBF-E TUF |
Gene namesi | Name:RAP1 Synonyms:GRF1, TUF1 Ordered Locus Names:YNL216W ORF Names:N1310 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YNL216W. |
SGDi | S000005160. RAP1. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000197112 | 1 – 827 | DNA-binding protein RAP1Add BLAST | 827 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 486 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 731 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P11938. |
PaxDbi | P11938. |
PRIDEi | P11938. |
PTM databases
iPTMneti | P11938. |
Interactioni
Subunit structurei
RIF1, SIR3 and SIR4 associate with RAP1 C-terminus in vivo. Interacts with a GCR1 homodimer.1 Publication
Binary interactionsi
GO - Molecular functioni
- TFIID-class transcription factor binding Source: SGD
Protein-protein interaction databases
BioGridi | 35620. 698 interactors. |
DIPi | DIP-851N. |
IntActi | P11938. 60 interactors. |
MINTi | P11938. |
STRINGi | 4932.YNL216W. |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Helixi | 365 – 376 | Combined sources | 12 | |
Helixi | 379 – 381 | Combined sources | 3 | |
Helixi | 386 – 391 | Combined sources | 6 | |
Turni | 392 – 394 | Combined sources | 3 | |
Helixi | 400 – 409 | Combined sources | 10 | |
Helixi | 412 – 414 | Combined sources | 3 | |
Beta strandi | 437 – 440 | Combined sources | 4 | |
Helixi | 451 – 470 | Combined sources | 20 | |
Beta strandi | 474 – 476 | Combined sources | 3 | |
Beta strandi | 509 – 511 | Combined sources | 3 | |
Helixi | 525 – 532 | Combined sources | 8 | |
Turni | 533 – 535 | Combined sources | 3 | |
Helixi | 538 – 547 | Combined sources | 10 | |
Helixi | 549 – 552 | Combined sources | 4 | |
Helixi | 554 – 562 | Combined sources | 9 | |
Turni | 595 – 597 | Combined sources | 3 | |
Helixi | 681 – 684 | Combined sources | 4 | |
Helixi | 688 – 690 | Combined sources | 3 | |
Turni | 700 – 702 | Combined sources | 3 | |
Helixi | 705 – 708 | Combined sources | 4 | |
Helixi | 713 – 724 | Combined sources | 12 | |
Turni | 730 – 732 | Combined sources | 3 | |
Helixi | 733 – 744 | Combined sources | 12 | |
Helixi | 748 – 757 | Combined sources | 10 | |
Turni | 758 – 760 | Combined sources | 3 | |
Helixi | 762 – 764 | Combined sources | 3 | |
Helixi | 765 – 775 | Combined sources | 11 | |
Beta strandi | 781 – 783 | Combined sources | 3 | |
Helixi | 789 – 796 | Combined sources | 8 | |
Helixi | 800 – 810 | Combined sources | 11 | |
Helixi | 812 – 823 | Combined sources | 12 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1IGN | X-ray | 2.25 | A/B | 353-598 | [»] | |
2L42 | NMR | - | A | 116-212 | [»] | |
3CZ6 | X-ray | 1.85 | A/B | 672-827 | [»] | |
3OWT | X-ray | 2.00 | A/B | 672-827 | [»] | |
3UKG | X-ray | 2.95 | A | 360-601 | [»] | |
4BJ5 | X-ray | 3.29 | C/E | 627-827 | [»] | |
4BJ6 | X-ray | 3.26 | C/E | 627-827 | [»] | |
4BJT | X-ray | 1.61 | A/B/C | 627-827 | [»] | |
4GFB | X-ray | 2.99 | A | 358-602 | [»] | |
DisProti | DP00020. | |||||
ProteinModelPortali | P11938. | |||||
SMRi | P11938. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P11938. |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 121 – 208 | BRCTPROSITE-ProRule annotationAdd BLAST | 88 | |
Domaini | 355 – 415 | HTH myb-typePROSITE-ProRule annotationAdd BLAST | 61 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 630 – 695 | Activation domainAdd BLAST | 66 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 71 – 74 | Poly-Asp | 4 | |
Compositional biasi | 81 – 84 | Poly-Thr | 4 | |
Compositional biasi | 313 – 320 | Poly-Asn | 8 | |
Compositional biasi | 614 – 627 | Poly-AlaAdd BLAST | 14 |
Sequence similaritiesi
Belongs to the RAP1 family.Curated
Phylogenomic databases
HOGENOMi | HOG000115833. |
InParanoidi | P11938. |
KOi | K09426. |
OMAi | EISHYVP. |
OrthoDBi | EOG092C4GZO. |
Family and domain databases
CDDi | cd00027. BRCT. 1 hit. |
Gene3Di | 1.10.10.2170. 1 hit. 3.40.50.10190. 1 hit. |
InterProi | View protein in InterPro IPR001357. BRCT_dom. IPR036420. BRCT_dom_sf. IPR009057. Homeobox-like_sf. IPR017930. Myb_dom. IPR021661. Rap1_C. IPR038104. Rap1_C_sf. IPR015280. Rap1_DNA-bd. IPR001005. SANT/Myb. |
Pfami | View protein in Pfam PF16589. BRCT_2. 1 hit. PF00249. Myb_DNA-binding. 1 hit. PF09197. Rap1-DNA-bind. 1 hit. PF11626. Rap1_C. 1 hit. |
SMARTi | View protein in SMART SM00292. BRCT. 1 hit. SM00717. SANT. 1 hit. |
SUPFAMi | SSF46689. SSF46689. 2 hits. SSF52113. SSF52113. 1 hit. |
PROSITEi | View protein in PROSITE PS50172. BRCT. 1 hit. PS51294. HTH_MYB. 1 hit. |
i Sequence
Sequence statusi: Complete.
P11938-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSPDDFETA PAEYVDALDP SMVVVDSGSA AVTAPSDSAA EVKANQNEEN
60 70 80 90 100
TGATAAETSE KVDQTEVEKK DDDDTTEVGV TTTTPSIADT AATANIASTS
110 120 130 140 150
GASVTEPTTD DTAADEKKEQ VSGPPLSNMK FYLNRDADAH DSLNDIDQLA
160 170 180 190 200
RLIRANGGEV LDSKPRESKE NVFIVSPYNH TNLPTVTPTY IKACCQSNSL
210 220 230 240 250
LNMENYLVPY DNFREVVDSR LQEESHSNGV DNSNSNSDNK DSIRPKTEII
260 270 280 290 300
STNTNGATED STSEKVMVDA EQQARLQEQA QLLRQHVSST ASITSGGHND
310 320 330 340 350
LVQIEQPQKD TSNNNNSNVN DEDNDLLTQD NNPQTADEGN ASFQAQRSMI
360 370 380 390 400
SRGALPSHNK ASFTDEEDEF ILDVVRKNPT RRTTHTLYDE ISHYVPNHTG
410 420 430 440 450
NSIRHRFRVY LSKRLEYVYE VDKFGKLVRD DDGNLIKTKV LPPSIKRKFS
460 470 480 490 500
ADEDYTLAIA VKKQFYRDLF QIDPDTGRSL ITDEDTPTAI ARRNMTMDPN
510 520 530 540 550
HVPGSEPNFA AYRTQSRRGP IAREFFKHFA EEHAAHTENA WRDRFRKFLL
560 570 580 590 600
AYGIDDYISY YEAEKAQNRE PEPMKNLTNR PKRPGVPTPG NYNSAAKRAR
610 620 630 640 650
NYSSQRNVQP TANAASANAA AAAAAAASNS YAIPENELLD EDTMNFISSL
660 670 680 690 700
KNDLSNISNS LPFEYPHEIA EAIRSDFSNE DIYDNIDPDT ISFPPKIATT
710 720 730 740 750
DLFLPLFFHF GSTRQFMDKL HEVISGDYEP SQAEKLVQDL CDETGIRKNF
760 770 780 790 800
STSILTCLSG DLMVFPRYFL NMFKDNVNPP PNVPGIWTHD DDESLKSNDQ
810 820
EQIRKLVKKH GTGRMEMRKR FFEKDLL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 672 | A → R in AAA18404 (PubMed:3315231).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M18068 Unassigned DNA. Translation: AAA18404.1. X78898 Genomic DNA. Translation: CAA55491.1. Z71492 Genomic DNA. Translation: CAA96118.1. BK006947 Genomic DNA. Translation: DAA10340.1. |
PIRi | S50714. |
RefSeqi | NP_014183.1. NM_001183054.1. |
Genome annotation databases
EnsemblFungii | YNL216W; YNL216W; YNL216W. |
GeneIDi | 855505. |
KEGGi | sce:YNL216W. |
Similar proteinsi
Entry informationi
Entry namei | RAP1_YEAST | |
Accessioni | P11938Primary (citable) accession number: P11938 Secondary accession number(s): D6W0X4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | February 1, 1995 | |
Last modified: | March 28, 2018 | |
This is version 188 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |