P11937 (DNAS1_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyribonuclease-1
EC=3.1.21.1

Alternative name(s):
Deoxyribonuclease I
Short name=DNase I

Gene names

Name:	DNASE1
Synonyms:	DNL1

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization By similarity.
Catalytic activity	Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products.
Cofactor	Divalent cations. Prefers calcium or magnesium.
Subcellular location	Secreted. Nucleus envelope. Note: Secretory protein, stored in zymogen granules and found in the nuclear envelope.
Sequence similarities	Belongs to the DNase I family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Nucleus Secreted
Ligand	Actin-binding Calcium
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	DNA catabolic process Inferred from electronic annotation. Source: InterPro apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear envelope Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	deoxyribonuclease I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Deoxyribonuclease-1		PRO_0000145099
Sites
	Active site	78	1	By similarity
	Active site	134	1	By similarity
Amino acid modifications
	Glycosylation	18	1	N-linked (GlcNAc...)
	Disulfide bond	101 ↔ 104		By similarity
	Disulfide bond	173 ↔ 209		Essential for enzymatic activity By similarity
Natural variations
	Natural variant	163	1	I → V in minor variant.

Sequences

	Sequence		Length	Mass (Da)
	P11937 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: B1B4D2C50DA3BF20	FASTA	260	28,939
	10 20 30 40 50 60 LKIAAFNIRT FGETKMSNAT LSSYIVRILR RYDIALIEQV RDSHLVAVGK LLDDLNQDDP 70 80 90 100 110 120 NSYHYVVSEP LGRNSYKERY LFVFRPNKVS VLDTYQYDDG CESCGNDSFS REPAVVKFSS 130 140 150 160 170 180 PSTKVKAFAI VPLHSAPSDA VAEINSLYDV YLDVQQKWDL NDIMLMGDFN ADCSYVTSSQ 190 200 210 220 230 240 WSSIRLRTSS TFQWLIPDSA DTTATSTNCA YDRIVVAGSL LQSSVVGPSA VPFDFQAAYG 250 260 LSNEMALAIS DHYPVEVTLT

References

[1]

"Comparison of the three primary structures of deoxyribonuclease isolated from bovine, ovine, and porcine pancreas. Derivation of the amino acid sequence of ovine DNase and revision of the previously published amino acid sequence of bovine DNase." Paudel H.K., Liao T.-H. J. Biol. Chem. 261:16012-16017(1986) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Pancreas.

Cross-references

Sequence databases
PIR	B26325.
3D structure databases
ProteinModelPortal	P11937.
SMR	P11937. Positions 1-260.
ModBase	Search...
Proteomic databases
PRIDE	P11937.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG051368.
Family and domain databases
InterPro	IPR018057. Deoxyribonuclease-1_AS. IPR016202. DNase_I-like. IPR008185. DNase_I_euk. IPR005135. Endo/exonuclease/phosphatase. [Graphical view]
PANTHER	PTHR11371. PTHR11371. 1 hit.
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
PIRSF	PIRSF000988. DNase_I_euk. 1 hit.
PRINTS	PR00130. DNASEI.
SMART	SM00476. DNaseIc. 1 hit. [Graphical view]
SUPFAM	SSF56219. Exo_endo_phos. 1 hit.
PROSITE	PS00919. DNASE_I_1. 1 hit. PS00918. DNASE_I_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAS1_SHEEP

Accession

Primary (citable) accession number: P11937

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program