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Protein

2'-5'-oligoadenylate synthase 1A

Gene

Oas1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L.1 Publication

Catalytic activityi

3 ATP = pppA2'p5'A2'p5'A + 2 diphosphate.

Cofactori

Mg2+By similarity

Enzyme regulationi

Produced as a latent enzyme which is activated by dsRNA generated during the course of viral infection. The dsRNA activator must be at least 15 nucleotides long, and no modification of the 2'-hydroxyl group is tolerated. ssRNA or dsDNA do not act as activators.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64ATPBy similarity1
Metal bindingi76Magnesium; catalyticBy similarity1
Metal bindingi78Magnesium; catalyticBy similarity1
Metal bindingi149Magnesium; catalyticBy similarity1
Binding sitei214ATPBy similarity1
Binding sitei231ATPBy similarity1

GO - Molecular functioni

  • 2'-5'-oligoadenylate synthetase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • double-stranded RNA binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • defense response to virus Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • negative regulation of viral process Source: MGI
  • purine nucleotide biosynthetic process Source: UniProtKB
  • response to virus Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-877300. Interferon gamma signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
2'-5'-oligoadenylate synthase 1A (EC:2.7.7.84)
Short name:
(2-5')oligo(A) synthase 1A
Short name:
2-5A synthase 1A
Alternative name(s):
p42 OAS
Gene namesi
Name:Oas1a
Synonyms:Oias1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2180860. Oas1a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Microsome, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001602601 – 3672'-5'-oligoadenylate synthase 1AAdd BLAST367

Proteomic databases

MaxQBiP11928.
PaxDbiP11928.
PeptideAtlasiP11928.
PRIDEiP11928.

PTM databases

PhosphoSitePlusiP11928.

Expressioni

Tissue specificityi

Expressed at high levels in the digestive tract and lymphoid organs.1 Publication

Inductioni

By type I interferon (IFN) and viruses.

Gene expression databases

BgeeiENSMUSG00000052776.
GenevisibleiP11928. MM.

Interactioni

Subunit structurei

Monomer. Homotetramer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
O891685EBI-9352099,EBI-10107989From a different organism.

Protein-protein interaction databases

BioGridi232938. 1 interactor.
IntActiP11928. 1 interactor.
STRINGi10090.ENSMUSP00000079198.

Structurei

3D structure databases

ProteinModelPortaliP11928.
SMRiP11928.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 61Interaction with dsRNABy similarityAdd BLAST48
Regioni201 – 211Interaction with dsRNABy similarityAdd BLAST11

Sequence similaritiesi

Belongs to the 2-5A synthase family.Curated

Phylogenomic databases

eggNOGiKOG0001. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00510000046406.
HOGENOMiHOG000022614.
HOVERGENiHBG000994.
InParanoidiP11928.
KOiK14216.
OMAiFQHQEVS.
OrthoDBiEOG091G0160.
TreeFamiTF329749.

Family and domain databases

Gene3Di1.10.1410.20. 1 hit.
InterProiIPR006117. 2-5-oligoadenylate_synth_CS.
IPR006116. 2-5-oligoadenylate_synth_N.
IPR018952. 2-5-oligoAdlate_synth_1_dom2/C.
IPR026774. 2-5A_synthase.
IPR002934. Polymerase_NTP_transf_dom.
[Graphical view]
PANTHERiPTHR11258. PTHR11258. 1 hit.
PfamiPF01909. NTP_transf_2. 1 hit.
PF10421. OAS1_C. 1 hit.
[Graphical view]
PROSITEiPS00832. 25A_SYNTH_1. 1 hit.
PS00833. 25A_SYNTH_2. 1 hit.
PS50152. 25A_SYNTH_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11928-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHGLRSIPA WTLDKFIEDY LLPDTTFGAD VKSAVNVVCD FLKERCFQGA
60 70 80 90 100
AHPVRVSKVV KGGSSGKGTT LKGKSDADLV VFLNNLTSFE DQLNRRGEFI
110 120 130 140 150
KEIKKQLYEV QHERRFRVKF EVQSSWWPNA RSLSFKLSAP HLHQEVEFDV
160 170 180 190 200
LPAFDVLGHV NTSSKPDPRI YAILIEECTS LGKDGEFSTC FTELQRNFLK
210 220 230 240 250
QRPTKLKSLI RLVKHWYQLC KEKLGKPLPP QYALELLTVF AWEQGNGCYE
260 270 280 290 300
FNTAQGFRTV LELVINYQHL RIYWTKYYDF QHQEVSKYLH RQLRKARPVI
310 320 330 340 350
LDPADPTGNV AGGNPEGWRR LAEEADVWLW YPCFIKKDGS RVSSWDVPTV
360
VPVPFEQVEE NWTCILL
Length:367
Mass (Da):42,429
Last modified:July 27, 2011 - v2
Checksum:iAE5946D5807776A4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti74K → R in CAA28620 (PubMed:3808949).Curated1
Sequence conflicti74K → R in AAA37116 (PubMed:2171206).Curated1
Sequence conflicti176E → W in CAA41105 (PubMed:1709495).Curated1
Sequence conflicti240F → Y in CAA41105 (PubMed:1709495).Curated1
Sequence conflicti249Y → N in CAA41105 (PubMed:1709495).Curated1
Sequence conflicti283Q → K in CAA41105 (PubMed:1709495).Curated1
Sequence conflicti329L → V in AAH57878 (PubMed:15489334).Curated1
Sequence conflicti329L → V in AAH13715 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04958 mRNA. Translation: CAA28620.1.
M33863 mRNA. Translation: AAA37116.1.
AK149613 mRNA. Translation: BAE28990.1.
AC015535 Genomic DNA. No translation available.
CH466529 Genomic DNA. Translation: EDL19743.1.
BC013715 mRNA. Translation: AAH13715.1.
BC057878 mRNA. Translation: AAH57878.1.
X58077 mRNA. Translation: CAA41105.1.
CCDSiCCDS19630.1.
PIRiA24725. SYMSO1.
S15660.
RefSeqiNP_660212.2. NM_145211.2.
UniGeneiMm.14301.
Mm.389688.

Genome annotation databases

EnsembliENSMUST00000080322; ENSMUSP00000079198; ENSMUSG00000052776.
GeneIDi246730.
KEGGimmu:246730.
UCSCiuc008zij.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04958 mRNA. Translation: CAA28620.1.
M33863 mRNA. Translation: AAA37116.1.
AK149613 mRNA. Translation: BAE28990.1.
AC015535 Genomic DNA. No translation available.
CH466529 Genomic DNA. Translation: EDL19743.1.
BC013715 mRNA. Translation: AAH13715.1.
BC057878 mRNA. Translation: AAH57878.1.
X58077 mRNA. Translation: CAA41105.1.
CCDSiCCDS19630.1.
PIRiA24725. SYMSO1.
S15660.
RefSeqiNP_660212.2. NM_145211.2.
UniGeneiMm.14301.
Mm.389688.

3D structure databases

ProteinModelPortaliP11928.
SMRiP11928.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi232938. 1 interactor.
IntActiP11928. 1 interactor.
STRINGi10090.ENSMUSP00000079198.

PTM databases

PhosphoSitePlusiP11928.

Proteomic databases

MaxQBiP11928.
PaxDbiP11928.
PeptideAtlasiP11928.
PRIDEiP11928.

Protocols and materials databases

DNASUi246730.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000080322; ENSMUSP00000079198; ENSMUSG00000052776.
GeneIDi246730.
KEGGimmu:246730.
UCSCiuc008zij.2. mouse.

Organism-specific databases

CTDi246730.
MGIiMGI:2180860. Oas1a.

Phylogenomic databases

eggNOGiKOG0001. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00510000046406.
HOGENOMiHOG000022614.
HOVERGENiHBG000994.
InParanoidiP11928.
KOiK14216.
OMAiFQHQEVS.
OrthoDBiEOG091G0160.
TreeFamiTF329749.

Enzyme and pathway databases

ReactomeiR-MMU-877300. Interferon gamma signaling.

Miscellaneous databases

PROiP11928.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052776.
GenevisibleiP11928. MM.

Family and domain databases

Gene3Di1.10.1410.20. 1 hit.
InterProiIPR006117. 2-5-oligoadenylate_synth_CS.
IPR006116. 2-5-oligoadenylate_synth_N.
IPR018952. 2-5-oligoAdlate_synth_1_dom2/C.
IPR026774. 2-5A_synthase.
IPR002934. Polymerase_NTP_transf_dom.
[Graphical view]
PANTHERiPTHR11258. PTHR11258. 1 hit.
PfamiPF01909. NTP_transf_2. 1 hit.
PF10421. OAS1_C. 1 hit.
[Graphical view]
PROSITEiPS00832. 25A_SYNTH_1. 1 hit.
PS00833. 25A_SYNTH_2. 1 hit.
PS50152. 25A_SYNTH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOAS1A_MOUSE
AccessioniPrimary (citable) accession number: P11928
Secondary accession number(s): F2Z419
, Q3UEC1, Q64440, Q91VI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.