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Protein

Non-specific lipid-transfer protein

Gene

Scp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates in vitro the transfer of all common phospholipids, cholesterol and gangliosides between membranes. May play a role in regulating steroidogenesis.

Catalytic activityi

3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanoyl-CoA + propanoyl-CoA = CoA + 3-alpha,7-alpha,12-alpha-trihydroxy-24-oxo-5-beta-cholestanoyl-CoA.

GO - Molecular functioni

  • acetyl-CoA C-acyltransferase activity Source: RGD
  • cholesterol transporter activity Source: RGD
  • phosphatidylcholine transporter activity Source: RGD
  • phosphatidylethanolamine transporter activity Source: RGD
  • propanoyl-CoA C-acyltransferase activity Source: UniProtKB-EC
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD
  • sterol binding Source: GO_Central

GO - Biological processi

  • aging Source: RGD
  • cellular response to cholesterol Source: RGD
  • metabolic process Source: InterPro
  • positive regulation of apoptotic process Source: RGD
  • positive regulation of cholesterol biosynthetic process Source: RGD
  • positive regulation of cholesterol import Source: RGD
  • positive regulation of intracellular cholesterol transport Source: GO_Central
  • positive regulation of steroid biosynthetic process Source: UniProtKB
  • response to estradiol Source: RGD
  • response to luteinizing hormone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14332.
SABIO-RKP11915.

Chemistry databases

SwissLipidsiSLP:000001233. [P11915-1]

Names & Taxonomyi

Protein namesi
Recommended name:
Non-specific lipid-transfer protein (EC:2.3.1.176)
Short name:
NSL-TP
Alternative name(s):
Propanoyl-CoA C-acyltransferase
SCP-chi
SCPX
Sterol carrier protein 2
Short name:
SCP-2
Sterol carrier protein X
Short name:
SCP-X
Gene namesi
Name:Scp2
Synonyms:Scp-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3642. Scp2.

Subcellular locationi

  • Cytoplasm
  • Mitochondrion

  • Note: Cytoplasmic in the liver and also associated with mitochondria especially in steroidogenic tissues.
Isoform SCPx :
  • Peroxisome

  • Note: Interaction with PEX5 is essential for peroxisomal import.By similarity
Isoform SCP2 :

GO - Cellular componenti

  • cytosol Source: RGD
  • endoplasmic reticulum Source: RGD
  • extrinsic component of membrane Source: RGD
  • extrinsic component of mitochondrial outer membrane Source: RGD
  • membrane Source: RGD
  • mitochondrion Source: RGD
  • nucleus Source: RGD
  • peroxisomal matrix Source: RGD
  • peroxisome Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000340971 – 547Non-specific lipid-transfer proteinAdd BLAST547

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3PhosphoserineCombined sources1
Modified residuei8PhosphoserineCombined sources1
Modified residuei40N6-succinyllysineBy similarity1
Modified residuei132N6-acetyllysine; alternateBy similarity1
Modified residuei132N6-succinyllysine; alternateBy similarity1
Modified residuei168N6-succinyllysineBy similarity1
Modified residuei177N6-acetyllysineBy similarity1
Modified residuei183N6-acetyllysine; alternateBy similarity1
Modified residuei183N6-succinyllysine; alternateBy similarity1
Modified residuei211N6-succinyllysineBy similarity1
Modified residuei282N6-succinyllysineBy similarity1
Modified residuei341N6-acetyllysine; alternateBy similarity1
Modified residuei341N6-succinyllysine; alternateBy similarity1
Modified residuei432N6-acetyllysine; alternateBy similarity1
Modified residuei432N6-succinyllysine; alternateBy similarity1
Modified residuei438N6-acetyllysine; alternateBy similarity1
Modified residuei438N6-succinyllysine; alternateBy similarity1
Modified residuei443N6-acetyllysine; alternateBy similarity1
Modified residuei443N6-succinyllysine; alternateBy similarity1
Modified residuei453N6-acetyllysine; alternateBy similarity1
Modified residuei453N6-succinyllysine; alternateBy similarity1
Modified residuei464N6-succinyllysineBy similarity1
Modified residuei470N6-acetyllysine; alternateBy similarity1
Modified residuei470N6-succinyllysine; alternateBy similarity1
Modified residuei479N6-succinyllysineBy similarity1
Modified residuei491N6-acetyllysineBy similarity1
Modified residuei492N6-succinyllysineBy similarity1
Modified residuei511N6-succinyllysineBy similarity1
Modified residuei516PhosphoserineBy similarity1
Modified residuei522N6-succinyllysineBy similarity1
Modified residuei534N6-succinyllysineBy similarity1
Modified residuei537PhosphoserineCombined sources1
Modified residuei544N6-succinyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP11915.
PRIDEiP11915.

PTM databases

iPTMnetiP11915.
PhosphoSitePlusiP11915.

Expressioni

Tissue specificityi

Liver > intestine > brain > lung, colon, stomach, spleen, kidney, heart and ovary.

Interactioni

Subunit structurei

Interacts with PEX5.By similarity

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

IntActiP11915. 1 interactor.
STRINGi10116.ENSRNOP00000015420.

Structurei

3D structure databases

ProteinModelPortaliP11915.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini433 – 543SCP2Add BLAST111

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi545 – 547Microbody targeting signalSequence analysis3

Sequence similaritiesi

In the N-terminal section; belongs to the thiolase family.Curated
Contains 1 SCP2 domain.Curated

Phylogenomic databases

eggNOGiKOG1406. Eukaryota.
KOG4170. Eukaryota.
ENOG410XPRW. LUCA.
HOGENOMiHOG000221741.
HOVERGENiHBG006506.
InParanoidiP11915.
KOiK08764.

Family and domain databases

Gene3Di3.30.1050.10. 1 hit.
3.40.47.10. 4 hits.
InterProiIPR003033. SCP2_sterol-bd_dom.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02036. SCP2. 1 hit.
PF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 2 hits.
SSF55718. SSF55718. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform SCPx (identifier: P11915-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPSVALNSPR LPRVFVVGVG MTKFMKPGGE NSRDYPDLAK EAGQKALADR
60 70 80 90 100
QIPYSAVEQA CVGYVYGEST CGQRAIYHSL GLTGIPIINV NNNCSTGSTA
110 120 130 140 150
LFMAQQLVQG GLANCVLALG FEKMEKGSLG TKYSDRSNPL EKHIDVLINK
160 170 180 190 200
YGMSACPFAP QLFGSAGKEH METYGTKVEH FAKIGWKNHK HSVNNPYSQF
210 220 230 240 250
QDEYSLDEIM KSRPVFDFLT VLQCCPTSDG AAAAIVSSEE FVQKHGLQSK
260 270 280 290 300
AVEIVAQEMV TDMPSTFEEK SVIKMVGYDM SKEAARKCYE KSGLGPSDVD
310 320 330 340 350
VIELHDCFST NELLTYEALG LCPEGQGGAL VDRGDNTYGG KWVINPSGGL
360 370 380 390 400
ISKGHPLGAT GLAQCAELCW QLRGEAGKRQ VPGAKVALQH NLGLGGAAVV
410 420 430 440 450
TLYRMGFPEA ASSFRTHQIS AAPTSSAGDG FKANLIFKEI EKKLEEEGEE
460 470 480 490 500
FVKKIGGIFA FKVKDGPGGK EATWVVDVKN GKGSVLPDSD KKADCTITMA
510 520 530 540
DSDLLALMTG KMNPQSAFFQ GKLKIAGNMG LAMKLQSLQL QPDKAKL
Length:547
Mass (Da):58,813
Last modified:August 1, 1991 - v3
Checksum:iD0D1B435D2DC6AFB
GO
Isoform SCP2 (identifier: P11915-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-404: Missing.

Note: Mitochondrial precursor. Contains a mitochondrial transit peptide at positions 405-424 (Potential).Curated
Show »
Length:143
Mass (Da):15,298
Checksum:iF80FB9B958462F52
GO

Sequence cautioni

The sequence AAA40623 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA43060 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12P → R in AAA40622 (PubMed:2034675).Curated1
Sequence conflicti12P → R in CAA43061 (PubMed:2034675).Curated1
Sequence conflicti12P → R in AAH81713 (PubMed:15489334).Curated1
Sequence conflicti50R → A in AAA40622 (PubMed:2034675).Curated1
Sequence conflicti50R → A in AAH81713 (PubMed:15489334).Curated1
Sequence conflicti265S → T in AAA42122 (PubMed:1985920).Curated1
Sequence conflicti427 – 428AG → SV AA sequence (PubMed:3395344).Curated2
Sequence conflicti436I → V AA sequence (PubMed:3395344).Curated1
Sequence conflicti446E → D AA sequence (PubMed:3395344).Curated1
Sequence conflicti450E → Q AA sequence (PubMed:3395344).Curated1
Sequence conflicti488D → N AA sequence (PubMed:3395344).Curated1
Sequence conflicti516S → T AA sequence (PubMed:3395344).Curated1
Sequence conflicti526A → N AA sequence (PubMed:3395344).Curated1
Sequence conflicti537S → N AA sequence (PubMed:3395344).Curated1
Sequence conflicti543D → G AA sequence (PubMed:3395344).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188971 – 404Missing in isoform SCP2. CuratedAdd BLAST404

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62763 mRNA. Translation: AAA40622.1.
M62763 mRNA. Translation: AAA40623.1. Different initiation.
M57454 mRNA. Translation: AAA42121.1.
M57453 mRNA. Translation: AAA42122.1.
M58287 mRNA. Translation: AAA41726.1.
BC081713 mRNA. Translation: AAH81713.1.
M34728 mRNA. Translation: AAA42120.1.
X60654 mRNA. Translation: CAA43060.1. Different initiation.
X60654 mRNA. Translation: CAA43061.1.
PIRiA39368.
RefSeqiNP_612517.2. NM_138508.4.
UniGeneiRn.31887.

Genome annotation databases

GeneIDi25541.
KEGGirno:25541.
UCSCiRGD:3642. rat. [P11915-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62763 mRNA. Translation: AAA40622.1.
M62763 mRNA. Translation: AAA40623.1. Different initiation.
M57454 mRNA. Translation: AAA42121.1.
M57453 mRNA. Translation: AAA42122.1.
M58287 mRNA. Translation: AAA41726.1.
BC081713 mRNA. Translation: AAH81713.1.
M34728 mRNA. Translation: AAA42120.1.
X60654 mRNA. Translation: CAA43060.1. Different initiation.
X60654 mRNA. Translation: CAA43061.1.
PIRiA39368.
RefSeqiNP_612517.2. NM_138508.4.
UniGeneiRn.31887.

3D structure databases

ProteinModelPortaliP11915.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP11915. 1 interactor.
STRINGi10116.ENSRNOP00000015420.

Chemistry databases

SwissLipidsiSLP:000001233. [P11915-1]

PTM databases

iPTMnetiP11915.
PhosphoSitePlusiP11915.

Proteomic databases

PaxDbiP11915.
PRIDEiP11915.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25541.
KEGGirno:25541.
UCSCiRGD:3642. rat. [P11915-1]

Organism-specific databases

CTDi6342.
RGDi3642. Scp2.

Phylogenomic databases

eggNOGiKOG1406. Eukaryota.
KOG4170. Eukaryota.
ENOG410XPRW. LUCA.
HOGENOMiHOG000221741.
HOVERGENiHBG006506.
InParanoidiP11915.
KOiK08764.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14332.
SABIO-RKP11915.

Miscellaneous databases

PROiP11915.

Family and domain databases

Gene3Di3.30.1050.10. 1 hit.
3.40.47.10. 4 hits.
InterProiIPR003033. SCP2_sterol-bd_dom.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02036. SCP2. 1 hit.
PF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 2 hits.
SSF55718. SSF55718. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNLTP_RAT
AccessioniPrimary (citable) accession number: P11915
Secondary accession number(s): Q63383, Q642G0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: August 1, 1991
Last modified: November 2, 2016
This is version 146 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.