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P11914

- MPPA_YEAST

UniProt

P11914 - MPPA_YEAST

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Protein

Mitochondrial-processing peptidase subunit alpha

Gene
MAS2, MIF2, YHR024C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cleaves presequences (transit peptides) from mitochondrial protein precursors. The cleavage sites typically contain an arginine at position -2 (in the N-terminal portion) from the scissible peptide bond in addition to other distal basic residues, and an aromatic residue at position +1.

Catalytic activityi

Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.

GO - Molecular functioni

  1. metal ion binding Source: InterPro
  2. metalloendopeptidase activity Source: InterPro
  3. protein binding Source: IntAct

GO - Biological processi

  1. protein processing involved in protein targeting to mitochondrion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16674.
YEAST:G3O-31085-MONOMER.
ReactomeiREACT_189012. Mitochondrial protein import.
SABIO-RKP11914.

Protein family/group databases

MEROPSiM16.971.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial-processing peptidase subunit alpha (EC:3.4.24.64)
Alternative name(s):
Alpha-MPP
Gene namesi
Name:MAS2
Synonyms:MIF2
Ordered Locus Names:YHR024C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR024c.
SGDiS000001066. MAS2.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: Reactome
  2. mitochondrial processing peptidase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1313Mitochondrion1 PublicationAdd
BLAST
Chaini14 – 482469Mitochondrial-processing peptidase subunit alphaPRO_0000026773Add
BLAST

Proteomic databases

MaxQBiP11914.
PaxDbiP11914.
PeptideAtlasiP11914.

Expressioni

Gene expression databases

GenevestigatoriP11914.

Interactioni

Subunit structurei

Heterodimer of alpha and beta subunits.

Binary interactionsi

WithEntry#Exp.IntActNotes
MAS1P105075EBI-11205,EBI-11212

Protein-protein interaction databases

BioGridi36455. 10 interactions.
DIPiDIP-2401N.
IntActiP11914. 6 interactions.
MINTiMINT-630854.
STRINGi4932.YHR024C.

Structurei

Secondary structure

1
482
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni15 – 184
Beta strandi20 – 234
Beta strandi29 – 346
Beta strandi38 – 4710
Helixi50 – 523
Turni54 – 596
Helixi60 – 667
Turni67 – 693
Beta strandi72 – 754
Helixi77 – 8610
Beta strandi91 – 955
Beta strandi100 – 1067
Helixi108 – 1103
Helixi111 – 12313
Helixi129 – 14618
Helixi150 – 16213
Turni163 – 1653
Helixi167 – 1693
Helixi176 – 1816
Helixi184 – 19411
Helixi197 – 1993
Beta strandi200 – 2078
Helixi209 – 22012
Beta strandi240 – 2445
Beta strandi251 – 2533
Beta strandi257 – 2648
Helixi273 – 28311
Beta strandi285 – 2895
Helixi301 – 3055
Turni306 – 3094
Beta strandi311 – 32212
Beta strandi327 – 3359
Helixi337 – 3426
Helixi343 – 35210
Turni353 – 3553
Helixi364 – 38118
Helixi385 – 39915
Helixi405 – 4139
Helixi417 – 42812
Beta strandi443 – 4486
Helixi450 – 4534
Helixi456 – 4627

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HR6X-ray2.50A/C/E/G14-482[»]
1HR7X-ray2.55A/C/E/G14-482[»]
1HR8X-ray2.70A/C/E/G14-482[»]
1HR9X-ray3.01A/C/E/G14-482[»]
ProteinModelPortaliP11914.
SMRiP11914. Positions 14-470.

Miscellaneous databases

EvolutionaryTraceiP11914.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M16 family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0612.
GeneTreeiENSGT00550000074666.
HOGENOMiHOG000206848.
KOiK01412.
OrthoDBiEOG7N63XC.

Family and domain databases

Gene3Di3.30.830.10. 2 hits.
InterProiIPR011249. Metalloenz_LuxS/M16.
IPR011237. Pept_M16_dom.
IPR011765. Pept_M16_N.
IPR001431. Pept_M16_Zn_BS.
IPR007863. Peptidase_M16_C.
[Graphical view]
PfamiPF00675. Peptidase_M16. 1 hit.
PF05193. Peptidase_M16_C. 1 hit.
[Graphical view]
SUPFAMiSSF63411. SSF63411. 2 hits.
PROSITEiPS00143. INSULINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11914-1 [UniParc]FASTAAdd to Basket

« Hide

MLRNGVQRLY SNIARTDNFK LSSLANGLKV ATSNTPGHFS ALGLYIDAGS    50
RFEGRNLKGC THILDRLAFK STEHVEGRAM AETLELLGGN YQCTSSRENL 100
MYQASVFNQD VGKMLQLMSE TVRFPKITEQ ELQEQKLSAE YEIDEVWMKP 150
ELVLPELLHT AAYSGETLGS PLICPRELIP SISKYYLLDY RNKFYTPENT 200
VAAFVGVPHE KALELTEKYL GDWQSTHPPI TKKVAQYTGG ESCIPPAPVF 250
GNLPELFHIQ IGFEGLPIDH PDIYALATLQ TLLGGGGSFS AGGPGKGMYS 300
RLYTHVLNQY YFVENCVAFN HSYSDSGIFG ISLSCIPQAA PQAVEVIAQQ 350
MYNTFANKDL RLTEDEVSRA KNQLKSSLLM NLESKLVELE DMGRQVLMHG 400
RKIPVNEMIS KIEDLKPDDI SRVAEMIFTG NVNNAGNGKG RATVVMQGDR 450
GSFGDVENVL KAYGLGNSSS SKNDSPKKKG WF 482
Length:482
Mass (Da):53,338
Last modified:October 1, 1989 - v1
Checksum:i0FDCD157388A56C7
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti235 – 2351A → P in CAA32262. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X13455 Genomic DNA. Translation: CAA31804.1.
X14105 Genomic DNA. Translation: CAA32262.1.
U10399 Genomic DNA. Translation: AAB68877.1.
BK006934 Genomic DNA. Translation: DAA06715.1.
PIRiS05738. ZPBY.
RefSeqiNP_011889.1. NM_001179154.1.

Genome annotation databases

EnsemblFungiiYHR024C; YHR024C; YHR024C.
GeneIDi856419.
KEGGisce:YHR024C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X13455 Genomic DNA. Translation: CAA31804.1 .
X14105 Genomic DNA. Translation: CAA32262.1 .
U10399 Genomic DNA. Translation: AAB68877.1 .
BK006934 Genomic DNA. Translation: DAA06715.1 .
PIRi S05738. ZPBY.
RefSeqi NP_011889.1. NM_001179154.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1HR6 X-ray 2.50 A/C/E/G 14-482 [» ]
1HR7 X-ray 2.55 A/C/E/G 14-482 [» ]
1HR8 X-ray 2.70 A/C/E/G 14-482 [» ]
1HR9 X-ray 3.01 A/C/E/G 14-482 [» ]
ProteinModelPortali P11914.
SMRi P11914. Positions 14-470.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36455. 10 interactions.
DIPi DIP-2401N.
IntActi P11914. 6 interactions.
MINTi MINT-630854.
STRINGi 4932.YHR024C.

Protein family/group databases

MEROPSi M16.971.

Proteomic databases

MaxQBi P11914.
PaxDbi P11914.
PeptideAtlasi P11914.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR024C ; YHR024C ; YHR024C .
GeneIDi 856419.
KEGGi sce:YHR024C.

Organism-specific databases

CYGDi YHR024c.
SGDi S000001066. MAS2.

Phylogenomic databases

eggNOGi COG0612.
GeneTreei ENSGT00550000074666.
HOGENOMi HOG000206848.
KOi K01412.
OrthoDBi EOG7N63XC.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16674.
YEAST:G3O-31085-MONOMER.
Reactomei REACT_189012. Mitochondrial protein import.
SABIO-RK P11914.

Miscellaneous databases

EvolutionaryTracei P11914.
NextBioi 981985.
PROi P11914.

Gene expression databases

Genevestigatori P11914.

Family and domain databases

Gene3Di 3.30.830.10. 2 hits.
InterProi IPR011249. Metalloenz_LuxS/M16.
IPR011237. Pept_M16_dom.
IPR011765. Pept_M16_N.
IPR001431. Pept_M16_Zn_BS.
IPR007863. Peptidase_M16_C.
[Graphical view ]
Pfami PF00675. Peptidase_M16. 1 hit.
PF05193. Peptidase_M16_C. 1 hit.
[Graphical view ]
SUPFAMi SSF63411. SSF63411. 2 hits.
PROSITEi PS00143. INSULINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The processing peptidase of yeast mitochondria: the two co-operating components MPP and PEP are structurally related."
    Pollock R.A., Hartl F.-U., Cheng M.Y., Ostermann J., Horwich A., Neupert W.
    EMBO J. 7:3493-3500(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: MC3.
  2. "Import of proteins into yeast mitochondria: the nuclear MAS2 gene encodes a component of the processing protease that is homologous to the MAS1-encoded subunit."
    Jensen R.E., Yaffe M.P.
    EMBO J. 7:3863-3871(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The MAS-encoded processing protease of yeast mitochondria. Interaction of the purified enzyme with signal peptides and a purified precursor protein."
    Yang M., Geli V., Oppliger W., Suda K., James P., Schatz G.
    J. Biol. Chem. 266:6416-6423(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 14-22.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Crystal structures of mitochondrial processing peptidase reveal the mode for specific cleavage of import signal sequences."
    Taylor A.B., Smith B.S., Kitada S., Kojima K., Miyaura H., Otwinowski Z., Ito A., Deisenhofer J.
    Structure 9:615-625(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 45-482 IN COMPLEX WITH SUBSTRATES.

Entry informationi

Entry nameiMPPA_YEAST
AccessioniPrimary (citable) accession number: P11914
Secondary accession number(s): D3DKX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: September 3, 2014
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 31400 molecules/cell in log phase SD medium.

Caution

Does not seem to have a protease activity as it lack the zinc-binding site.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

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