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P11914

- MPPA_YEAST

UniProt

P11914 - MPPA_YEAST

Protein

Mitochondrial-processing peptidase subunit alpha

Gene

MAS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 150 (01 Oct 2014)
      Sequence version 1 (01 Oct 1989)
      Previous versions | rss
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    Functioni

    Cleaves presequences (transit peptides) from mitochondrial protein precursors. The cleavage sites typically contain an arginine at position -2 (in the N-terminal portion) from the scissible peptide bond in addition to other distal basic residues, and an aromatic residue at position +1.

    Catalytic activityi

    Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.

    GO - Molecular functioni

    1. metal ion binding Source: InterPro
    2. metalloendopeptidase activity Source: InterPro
    3. protein binding Source: IntAct

    GO - Biological processi

    1. protein processing involved in protein targeting to mitochondrion Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16674.
    YEAST:G3O-31085-MONOMER.
    ReactomeiREACT_189012. Mitochondrial protein import.
    SABIO-RKP11914.

    Protein family/group databases

    MEROPSiM16.971.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial-processing peptidase subunit alpha (EC:3.4.24.64)
    Alternative name(s):
    Alpha-MPP
    Gene namesi
    Name:MAS2
    Synonyms:MIF2
    Ordered Locus Names:YHR024C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR024c.
    SGDiS000001066. MAS2.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: Reactome
    2. mitochondrial processing peptidase complex Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1313Mitochondrion1 PublicationAdd
    BLAST
    Chaini14 – 482469Mitochondrial-processing peptidase subunit alphaPRO_0000026773Add
    BLAST

    Proteomic databases

    MaxQBiP11914.
    PaxDbiP11914.
    PeptideAtlasiP11914.

    Expressioni

    Gene expression databases

    GenevestigatoriP11914.

    Interactioni

    Subunit structurei

    Heterodimer of alpha and beta subunits.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MAS1P105075EBI-11205,EBI-11212

    Protein-protein interaction databases

    BioGridi36455. 10 interactions.
    DIPiDIP-2401N.
    IntActiP11914. 6 interactions.
    MINTiMINT-630854.
    STRINGi4932.YHR024C.

    Structurei

    Secondary structure

    1
    482
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni15 – 184
    Beta strandi20 – 234
    Beta strandi29 – 346
    Beta strandi38 – 4710
    Helixi50 – 523
    Turni54 – 596
    Helixi60 – 667
    Turni67 – 693
    Beta strandi72 – 754
    Helixi77 – 8610
    Beta strandi91 – 955
    Beta strandi100 – 1067
    Helixi108 – 1103
    Helixi111 – 12313
    Helixi129 – 14618
    Helixi150 – 16213
    Turni163 – 1653
    Helixi167 – 1693
    Helixi176 – 1816
    Helixi184 – 19411
    Helixi197 – 1993
    Beta strandi200 – 2078
    Helixi209 – 22012
    Beta strandi240 – 2445
    Beta strandi251 – 2533
    Beta strandi257 – 2648
    Helixi273 – 28311
    Beta strandi285 – 2895
    Helixi301 – 3055
    Turni306 – 3094
    Beta strandi311 – 32212
    Beta strandi327 – 3359
    Helixi337 – 3426
    Helixi343 – 35210
    Turni353 – 3553
    Helixi364 – 38118
    Helixi385 – 39915
    Helixi405 – 4139
    Helixi417 – 42812
    Beta strandi443 – 4486
    Helixi450 – 4534
    Helixi456 – 4627

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1HR6X-ray2.50A/C/E/G14-482[»]
    1HR7X-ray2.55A/C/E/G14-482[»]
    1HR8X-ray2.70A/C/E/G14-482[»]
    1HR9X-ray3.01A/C/E/G14-482[»]
    ProteinModelPortaliP11914.
    SMRiP11914. Positions 14-470.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP11914.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M16 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0612.
    GeneTreeiENSGT00550000074666.
    HOGENOMiHOG000206848.
    KOiK01412.
    OrthoDBiEOG7N63XC.

    Family and domain databases

    Gene3Di3.30.830.10. 2 hits.
    InterProiIPR011249. Metalloenz_LuxS/M16.
    IPR011237. Pept_M16_dom.
    IPR011765. Pept_M16_N.
    IPR001431. Pept_M16_Zn_BS.
    IPR007863. Peptidase_M16_C.
    [Graphical view]
    PfamiPF00675. Peptidase_M16. 1 hit.
    PF05193. Peptidase_M16_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF63411. SSF63411. 2 hits.
    PROSITEiPS00143. INSULINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P11914-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRNGVQRLY SNIARTDNFK LSSLANGLKV ATSNTPGHFS ALGLYIDAGS    50
    RFEGRNLKGC THILDRLAFK STEHVEGRAM AETLELLGGN YQCTSSRENL 100
    MYQASVFNQD VGKMLQLMSE TVRFPKITEQ ELQEQKLSAE YEIDEVWMKP 150
    ELVLPELLHT AAYSGETLGS PLICPRELIP SISKYYLLDY RNKFYTPENT 200
    VAAFVGVPHE KALELTEKYL GDWQSTHPPI TKKVAQYTGG ESCIPPAPVF 250
    GNLPELFHIQ IGFEGLPIDH PDIYALATLQ TLLGGGGSFS AGGPGKGMYS 300
    RLYTHVLNQY YFVENCVAFN HSYSDSGIFG ISLSCIPQAA PQAVEVIAQQ 350
    MYNTFANKDL RLTEDEVSRA KNQLKSSLLM NLESKLVELE DMGRQVLMHG 400
    RKIPVNEMIS KIEDLKPDDI SRVAEMIFTG NVNNAGNGKG RATVVMQGDR 450
    GSFGDVENVL KAYGLGNSSS SKNDSPKKKG WF 482
    Length:482
    Mass (Da):53,338
    Last modified:October 1, 1989 - v1
    Checksum:i0FDCD157388A56C7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti235 – 2351A → P in CAA32262. (PubMed:3061808)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13455 Genomic DNA. Translation: CAA31804.1.
    X14105 Genomic DNA. Translation: CAA32262.1.
    U10399 Genomic DNA. Translation: AAB68877.1.
    BK006934 Genomic DNA. Translation: DAA06715.1.
    PIRiS05738. ZPBY.
    RefSeqiNP_011889.1. NM_001179154.1.

    Genome annotation databases

    EnsemblFungiiYHR024C; YHR024C; YHR024C.
    GeneIDi856419.
    KEGGisce:YHR024C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13455 Genomic DNA. Translation: CAA31804.1 .
    X14105 Genomic DNA. Translation: CAA32262.1 .
    U10399 Genomic DNA. Translation: AAB68877.1 .
    BK006934 Genomic DNA. Translation: DAA06715.1 .
    PIRi S05738. ZPBY.
    RefSeqi NP_011889.1. NM_001179154.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1HR6 X-ray 2.50 A/C/E/G 14-482 [» ]
    1HR7 X-ray 2.55 A/C/E/G 14-482 [» ]
    1HR8 X-ray 2.70 A/C/E/G 14-482 [» ]
    1HR9 X-ray 3.01 A/C/E/G 14-482 [» ]
    ProteinModelPortali P11914.
    SMRi P11914. Positions 14-470.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36455. 10 interactions.
    DIPi DIP-2401N.
    IntActi P11914. 6 interactions.
    MINTi MINT-630854.
    STRINGi 4932.YHR024C.

    Protein family/group databases

    MEROPSi M16.971.

    Proteomic databases

    MaxQBi P11914.
    PaxDbi P11914.
    PeptideAtlasi P11914.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR024C ; YHR024C ; YHR024C .
    GeneIDi 856419.
    KEGGi sce:YHR024C.

    Organism-specific databases

    CYGDi YHR024c.
    SGDi S000001066. MAS2.

    Phylogenomic databases

    eggNOGi COG0612.
    GeneTreei ENSGT00550000074666.
    HOGENOMi HOG000206848.
    KOi K01412.
    OrthoDBi EOG7N63XC.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16674.
    YEAST:G3O-31085-MONOMER.
    Reactomei REACT_189012. Mitochondrial protein import.
    SABIO-RK P11914.

    Miscellaneous databases

    EvolutionaryTracei P11914.
    NextBioi 981985.
    PROi P11914.

    Gene expression databases

    Genevestigatori P11914.

    Family and domain databases

    Gene3Di 3.30.830.10. 2 hits.
    InterProi IPR011249. Metalloenz_LuxS/M16.
    IPR011237. Pept_M16_dom.
    IPR011765. Pept_M16_N.
    IPR001431. Pept_M16_Zn_BS.
    IPR007863. Peptidase_M16_C.
    [Graphical view ]
    Pfami PF00675. Peptidase_M16. 1 hit.
    PF05193. Peptidase_M16_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF63411. SSF63411. 2 hits.
    PROSITEi PS00143. INSULINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The processing peptidase of yeast mitochondria: the two co-operating components MPP and PEP are structurally related."
      Pollock R.A., Hartl F.-U., Cheng M.Y., Ostermann J., Horwich A., Neupert W.
      EMBO J. 7:3493-3500(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: MC3.
    2. "Import of proteins into yeast mitochondria: the nuclear MAS2 gene encodes a component of the processing protease that is homologous to the MAS1-encoded subunit."
      Jensen R.E., Yaffe M.P.
      EMBO J. 7:3863-3871(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The MAS-encoded processing protease of yeast mitochondria. Interaction of the purified enzyme with signal peptides and a purified precursor protein."
      Yang M., Geli V., Oppliger W., Suda K., James P., Schatz G.
      J. Biol. Chem. 266:6416-6423(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 14-22.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Crystal structures of mitochondrial processing peptidase reveal the mode for specific cleavage of import signal sequences."
      Taylor A.B., Smith B.S., Kitada S., Kojima K., Miyaura H., Otwinowski Z., Ito A., Deisenhofer J.
      Structure 9:615-625(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 45-482 IN COMPLEX WITH SUBSTRATES.

    Entry informationi

    Entry nameiMPPA_YEAST
    AccessioniPrimary (citable) accession number: P11914
    Secondary accession number(s): D3DKX1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 150 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 31400 molecules/cell in log phase SD medium.1 Publication

    Caution

    Does not seem to have a protease activity as it lack the zinc-binding site.Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3