Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P11914 (MPPA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 148. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial-processing peptidase subunit alpha

EC=3.4.24.64
Alternative name(s):
Alpha-MPP
Gene names
Name:MAS2
Synonyms:MIF2
Ordered Locus Names:YHR024C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length482 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cleaves presequences (transit peptides) from mitochondrial protein precursors. The cleavage sites typically contain an arginine at position -2 (in the N-terminal portion) from the scissible peptide bond in addition to other distal basic residues, and an aromatic residue at position +1.

Catalytic activity

Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.

Subunit structure

Heterodimer of alpha and beta subunits.

Subcellular location

Mitochondrion matrix.

Miscellaneous

Present with 31400 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the peptidase M16 family.

Caution

Does not seem to have a protease activity as it lack the zinc-binding site.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MAS1P105075EBI-11205,EBI-11212

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1313Mitochondrion Ref.5
Chain14 – 482469Mitochondrial-processing peptidase subunit alpha
PRO_0000026773

Experimental info

Sequence conflict2351A → P in CAA32262. Ref.2

Secondary structure

............................................................................. 482
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P11914 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: 0FDCD157388A56C7

FASTA48253,338
        10         20         30         40         50         60 
MLRNGVQRLY SNIARTDNFK LSSLANGLKV ATSNTPGHFS ALGLYIDAGS RFEGRNLKGC 

        70         80         90        100        110        120 
THILDRLAFK STEHVEGRAM AETLELLGGN YQCTSSRENL MYQASVFNQD VGKMLQLMSE 

       130        140        150        160        170        180 
TVRFPKITEQ ELQEQKLSAE YEIDEVWMKP ELVLPELLHT AAYSGETLGS PLICPRELIP 

       190        200        210        220        230        240 
SISKYYLLDY RNKFYTPENT VAAFVGVPHE KALELTEKYL GDWQSTHPPI TKKVAQYTGG 

       250        260        270        280        290        300 
ESCIPPAPVF GNLPELFHIQ IGFEGLPIDH PDIYALATLQ TLLGGGGSFS AGGPGKGMYS 

       310        320        330        340        350        360 
RLYTHVLNQY YFVENCVAFN HSYSDSGIFG ISLSCIPQAA PQAVEVIAQQ MYNTFANKDL 

       370        380        390        400        410        420 
RLTEDEVSRA KNQLKSSLLM NLESKLVELE DMGRQVLMHG RKIPVNEMIS KIEDLKPDDI 

       430        440        450        460        470        480 
SRVAEMIFTG NVNNAGNGKG RATVVMQGDR GSFGDVENVL KAYGLGNSSS SKNDSPKKKG 


WF 

« Hide

References

« Hide 'large scale' references
[1]"The processing peptidase of yeast mitochondria: the two co-operating components MPP and PEP are structurally related."
Pollock R.A., Hartl F.-U., Cheng M.Y., Ostermann J., Horwich A., Neupert W.
EMBO J. 7:3493-3500(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: MC3.
[2]"Import of proteins into yeast mitochondria: the nuclear MAS2 gene encodes a component of the processing protease that is homologous to the MAS1-encoded subunit."
Jensen R.E., Yaffe M.P.
EMBO J. 7:3863-3871(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"The MAS-encoded processing protease of yeast mitochondria. Interaction of the purified enzyme with signal peptides and a purified precursor protein."
Yang M., Geli V., Oppliger W., Suda K., James P., Schatz G.
J. Biol. Chem. 266:6416-6423(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 14-22.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Crystal structures of mitochondrial processing peptidase reveal the mode for specific cleavage of import signal sequences."
Taylor A.B., Smith B.S., Kitada S., Kojima K., Miyaura H., Otwinowski Z., Ito A., Deisenhofer J.
Structure 9:615-625(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 45-482 IN COMPLEX WITH SUBSTRATES.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X13455 Genomic DNA. Translation: CAA31804.1.
X14105 Genomic DNA. Translation: CAA32262.1.
U10399 Genomic DNA. Translation: AAB68877.1.
BK006934 Genomic DNA. Translation: DAA06715.1.
PIRZPBY. S05738.
RefSeqNP_011889.1. NM_001179154.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1HR6X-ray2.50A/C/E/G14-482[»]
1HR7X-ray2.55A/C/E/G14-482[»]
1HR8X-ray2.70A/C/E/G14-482[»]
1HR9X-ray3.01A/C/E/G14-482[»]
ProteinModelPortalP11914.
SMRP11914. Positions 14-470.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36455. 10 interactions.
DIPDIP-2401N.
IntActP11914. 6 interactions.
MINTMINT-630854.
STRING4932.YHR024C.

Protein family/group databases

MEROPSM16.971.

Proteomic databases

MaxQBP11914.
PaxDbP11914.
PeptideAtlasP11914.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR024C; YHR024C; YHR024C.
GeneID856419.
KEGGsce:YHR024C.

Organism-specific databases

CYGDYHR024c.
SGDS000001066. MAS2.

Phylogenomic databases

eggNOGCOG0612.
GeneTreeENSGT00550000074666.
HOGENOMHOG000206848.
KOK01412.
OrthoDBEOG7N63XC.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16674.
YEAST:G3O-31085-MONOMER.
ReactomeREACT_118590. Mitochondrial Protein Import (yeast).
SABIO-RKP11914.

Gene expression databases

GenevestigatorP11914.

Family and domain databases

Gene3D3.30.830.10. 2 hits.
InterProIPR011249. Metalloenz_LuxS/M16.
IPR011237. Pept_M16_dom.
IPR011765. Pept_M16_N.
IPR001431. Pept_M16_Zn_BS.
IPR007863. Peptidase_M16_C.
[Graphical view]
PfamPF00675. Peptidase_M16. 1 hit.
PF05193. Peptidase_M16_C. 1 hit.
[Graphical view]
SUPFAMSSF63411. SSF63411. 2 hits.
PROSITEPS00143. INSULINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP11914.
NextBio981985.
PROP11914.

Entry information

Entry nameMPPA_YEAST
AccessionPrimary (citable) accession number: P11914
Secondary accession number(s): D3DKX1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 11, 2014
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references