Reviewed, UniProtKB/Swiss-Prot P11913 (MPPB_NEUCR)

Names and origin

Protein names

Recommended name:
    Mitochondrial-processing peptidase subunit beta
    EC=3.4.24.64
Alternative name(s):
    Beta-MPP
    Ubiquinol-cytochrome-c reductase complex core protein I

Gene names

Name:	pep
ORF Names:	NCU02549

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cleaves presequences (transit peptides) from mitochondrial protein precursors. This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1.
Catalytic activity	Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Heterodimer of alpha and beta subunits.
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the peptidase M16 family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 28	28	Mitochondrion Ref.1
	Chain	29 – 476	448	Mitochondrial-processing peptidase subunit beta		PRO_0000026782
Regions
	Compositional bias	150 – 178	29	Asp/Glu-rich (acidic)
Sites
	Active site	87	1	Proton acceptor By similarity
	Metal binding	84	1	Zinc By similarity
	Metal binding	88	1	Zinc By similarity
	Metal binding	164	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P11913-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: BF3905A20D3945E4	FASTA	476	52,556
	10 20 30 40 50 60 MASRRLALNL AQGVKARAGG VINPFRRGLA TPHSGTGIKT QTTTLKNGLT VASQYSPYAQ 70 80 90 100 110 120 TSTVGMWIDA GSRAETDETN GTAHFLEHLA FKGTTKRTQQ QLELEIENMG AHLNAYTSRE 130 140 150 160 170 180 NTVYFAKALN EDVPKCVDIL QDILQNSKLE ESAIERERDV ILRESEEVEK QLEEVVFDHL 190 200 210 220 230 240 HATAYQHQPL GRTILGPREN IRDITRTELV NYIKNNYTAD RMVLVGAGGV PHEQLVEMAD 250 260 270 280 290 300 KYFSKLPATA PVSSASILSK KKPDFIGSDI RIRDDTIPTA NIAIAVEGVS WSDDDYFTGL 310 320 330 340 350 360 VTQAIVGNYD KALGNAPHQG SKLSGFVHKH DLATSFMSFS TSYSDTGLWG IYLVTDKLDR 370 380 390 400 410 420 VDDLVHFSLR EWTRLCSNVS EAEVERAKAQ LKASILLSLD GTTAVAEDIG RQIVTTGRRM 430 440 450 460 470 SPAEIERIID AVSAKDVMDF ANKKIWDQDI AISAVGSIEG LFDYARIRGD MSRNAF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mitochondrial protein import: identification of processing peptidase and of PEP, a processing enhancing protein." Hawlitschek G., Schneider H., Schmidt B., Tropschug M., Hartl F.-U., Neupert W. Cell 53:795-806(1988) [PubMed: 2967109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 29-34. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"A family of mitochondrial proteins involved in bioenergetICS and biogenesis." Schulte U., Arretz M., Schneider H., Tropschug M., Wachter E., Neupert W., Weiss H. Nature 339:147-149(1989) [PubMed: 2524007] [Abstract] Cited for: IDENTITY WITH CYTOCHROME C REDUCTASE CORE PROTEIN I.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M20928 mRNA. Translation: AAA33606.1. AABX02000006 Genomic DNA. Translation: EAA36444.1.
PIR	A29881.
RefSeq	XP_965680.1.
3D structure databases
HSSP	HSSP built from PDB template 1HR6 based on UniProtKB P10507.
ModBase	Search...
Protein family/group databases
MEROPS	M16.003. M16.980.
Genome annotation databases
GeneID	3881830.
KEGG	ncr:NCU02549.
NMPDR	fig|5141.1.peg.9681.
Enzyme and pathway databases
BioCyc	NCRA-XX3-01:NCRA-XX3-01-006297-MON.
BRENDA	3.4.24.64. 266.
Family and domain databases
InterPro	IPR011237. Pept_M16_core. IPR011765. Pept_M16_N. IPR001431. Pept_M16_Zn_BS. IPR007863. Peptidase_M16_C. [Graphical view]
Gene3D	G3DSA:3.30.830.10. Pept_M16_core. 2 hits.
Pfam	PF00675. Peptidase_M16. 1 hit. PF05193. Peptidase_M16_C. 1 hit. [Graphical view]
PROSITE	PS00143. INSULINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPPB_NEUCR

Accession

Primary (citable) accession number: P11913
Secondary accession number(s): Q7RVM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	June 16, 2009
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)