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Protein

Ribose-phosphate pyrophosphokinase 2

Gene

PRPS2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.

Catalytic activityi

ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Enzyme regulationi

Activated by magnesium and inorganic phosphate. Competitively or non-competitively inhibited by ADP, 2,3-bisphosphoglyceride or GDP.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi128 – 1281MagnesiumSequence Analysis
Metal bindingi130 – 1301MagnesiumSequence Analysis
Binding sitei130 – 1301ATPBy similarity
Metal bindingi139 – 1391MagnesiumSequence Analysis
Metal bindingi143 – 1431MagnesiumSequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi96 – 1016ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS02317-MONOMER.
ReactomeiREACT_850. 5-Phosphoribose 1-diphosphate biosynthesis.
UniPathwayiUPA00087; UER00172.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribose-phosphate pyrophosphokinase 2 (EC:2.7.6.1)
Alternative name(s):
PPRibP
Phosphoribosyl pyrophosphate synthase II
Short name:
PRS-II
Gene namesi
Name:PRPS2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:9465. PRPS2.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • ribose phosphate diphosphokinase complex Source: Ensembl
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33820.

Polymorphism and mutation databases

BioMutaiPRPS2.
DMDMi125583.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 318317Ribose-phosphate pyrophosphokinase 2PRO_0000141074Add
BLAST

Proteomic databases

MaxQBiP11908.
PaxDbiP11908.
PRIDEiP11908.

2D gel databases

REPRODUCTION-2DPAGEIPI00219617.
P11908.

PTM databases

PhosphoSiteiP11908.

Expressioni

Gene expression databases

BgeeiP11908.
CleanExiHS_PRPS2.
ExpressionAtlasiP11908. baseline and differential.
GenevestigatoriP11908.

Interactioni

Subunit structurei

Homodimer. The active form is probably a hexamer composed of 3 homodimers (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-4290895,EBI-4290895
NOTCH2NLQ7Z3S93EBI-4290895,EBI-945833
PRPS1P608915EBI-4290895,EBI-749195
PRPSAP1Q145585EBI-4290895,EBI-724449
WDYHV1Q96HA83EBI-4290895,EBI-741158

Protein-protein interaction databases

BioGridi111617. 17 interactions.
IntActiP11908. 7 interactions.
MINTiMINT-5000929.
STRINGi9606.ENSP00000381504.

Structurei

3D structure databases

ProteinModelPortaliP11908.
SMRiP11908. Positions 3-317.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni212 – 22716Binding of phosphoribosylpyrophosphateSequence AnalysisAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0462.
GeneTreeiENSGT00550000074583.
HOGENOMiHOG000210451.
HOVERGENiHBG001520.
InParanoidiP11908.
KOiK00948.
OMAiQMANEIK.
PhylomeDBiP11908.
TreeFamiTF106366.

Family and domain databases

Gene3Di3.40.50.2020. 2 hits.
HAMAPiMF_00583_B. RibP_PPkinase_B.
InterProiIPR000842. PRib_PP_synth_CS.
IPR029099. Pribosyltran_N.
IPR029057. PRTase-like.
IPR005946. Rib-P_diPkinase.
[Graphical view]
PfamiPF14572. Pribosyl_synth. 1 hit.
PF13793. Pribosyltran_N. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01251. ribP_PPkin. 1 hit.
PROSITEiPS00114. PRPP_SYNTHASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P11908-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPNIVLFSGS SHQDLSQRVA DRLGLELGKV VTKKFSNQET SVEIGESVRG
60 70 80 90 100
EDVYIIQSGC GEINDNLMEL LIMINACKIA SSSRVTAVIP CFPYARQDKK
110 120 130 140 150
DKSRAPISAK LVANMLSVAG ADHIITMDLH ASQIQGFFDI PVDNLYAEPA
160 170 180 190 200
VLQWIRENIA EWKNCIIVSP DAGGAKRVTS IADRLNVEFA LIHKERKKAN
210 220 230 240 250
EVDRMVLVGD VKDRVAILVD DMADTCGTIC HAADKLLSAG ATKVYAILTH
260 270 280 290 300
GIFSGPAISR INNAAFEAVV VTNTIPQEDK MKHCTKIQVI DISMILAEAI
310
RRTHNGESVS YLFSHVPL
Length:318
Mass (Da):34,769
Last modified:January 23, 2007 - v2
Checksum:i72A2873408250C31
GO
Isoform 2 (identifier: P11908-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     102-102: K → KVGE

Note: No experimental confirmation available.

Show »
Length:321
Mass (Da):35,054
Checksum:iEDD88B9D33A55A37
GO

Sequence cautioni

The sequence AAH30019.2 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei102 – 1021K → KVGE in isoform 2. 1 PublicationVSP_027769

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00971 mRNA. Translation: CAA68785.1.
BC030019 mRNA. Translation: AAH30019.2. Different initiation.
BC040483 mRNA. Translation: AAH40483.3.
BC110875 mRNA. Translation: AAI10876.2.
BC119662 mRNA. Translation: AAI19663.1.
BC119663 mRNA. Translation: AAI19664.1.
D28134 Genomic DNA. Translation: BAA05676.1.
CCDSiCCDS14150.1. [P11908-1]
CCDS43918.1. [P11908-2]
PIRiS02778. KIHUR2.
RefSeqiNP_001034180.1. NM_001039091.2. [P11908-2]
NP_002756.1. NM_002765.4. [P11908-1]
UniGeneiHs.654581.

Genome annotation databases

EnsembliENST00000380668; ENSP00000370043; ENSG00000101911. [P11908-1]
ENST00000398491; ENSP00000381504; ENSG00000101911. [P11908-2]
GeneIDi5634.
KEGGihsa:5634.
UCSCiuc004cva.3. human. [P11908-2]
uc004cvb.3. human. [P11908-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00971 mRNA. Translation: CAA68785.1.
BC030019 mRNA. Translation: AAH30019.2. Different initiation.
BC040483 mRNA. Translation: AAH40483.3.
BC110875 mRNA. Translation: AAI10876.2.
BC119662 mRNA. Translation: AAI19663.1.
BC119663 mRNA. Translation: AAI19664.1.
D28134 Genomic DNA. Translation: BAA05676.1.
CCDSiCCDS14150.1. [P11908-1]
CCDS43918.1. [P11908-2]
PIRiS02778. KIHUR2.
RefSeqiNP_001034180.1. NM_001039091.2. [P11908-2]
NP_002756.1. NM_002765.4. [P11908-1]
UniGeneiHs.654581.

3D structure databases

ProteinModelPortaliP11908.
SMRiP11908. Positions 3-317.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111617. 17 interactions.
IntActiP11908. 7 interactions.
MINTiMINT-5000929.
STRINGi9606.ENSP00000381504.

PTM databases

PhosphoSiteiP11908.

Polymorphism and mutation databases

BioMutaiPRPS2.
DMDMi125583.

2D gel databases

REPRODUCTION-2DPAGEIPI00219617.
P11908.

Proteomic databases

MaxQBiP11908.
PaxDbiP11908.
PRIDEiP11908.

Protocols and materials databases

DNASUi5634.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000380668; ENSP00000370043; ENSG00000101911. [P11908-1]
ENST00000398491; ENSP00000381504; ENSG00000101911. [P11908-2]
GeneIDi5634.
KEGGihsa:5634.
UCSCiuc004cva.3. human. [P11908-2]
uc004cvb.3. human. [P11908-1]

Organism-specific databases

CTDi5634.
GeneCardsiGC0XP012719.
HGNCiHGNC:9465. PRPS2.
MIMi311860. gene.
neXtProtiNX_P11908.
PharmGKBiPA33820.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0462.
GeneTreeiENSGT00550000074583.
HOGENOMiHOG000210451.
HOVERGENiHBG001520.
InParanoidiP11908.
KOiK00948.
OMAiQMANEIK.
PhylomeDBiP11908.
TreeFamiTF106366.

Enzyme and pathway databases

UniPathwayiUPA00087; UER00172.
BioCyciMetaCyc:HS02317-MONOMER.
ReactomeiREACT_850. 5-Phosphoribose 1-diphosphate biosynthesis.

Miscellaneous databases

ChiTaRSiPRPS2. human.
GenomeRNAii5634.
NextBioi21892.
PROiP11908.
SOURCEiSearch...

Gene expression databases

BgeeiP11908.
CleanExiHS_PRPS2.
ExpressionAtlasiP11908. baseline and differential.
GenevestigatoriP11908.

Family and domain databases

Gene3Di3.40.50.2020. 2 hits.
HAMAPiMF_00583_B. RibP_PPkinase_B.
InterProiIPR000842. PRib_PP_synth_CS.
IPR029099. Pribosyltran_N.
IPR029057. PRTase-like.
IPR005946. Rib-P_diPkinase.
[Graphical view]
PfamiPF14572. Pribosyl_synth. 1 hit.
PF13793. Pribosyltran_N. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01251. ribP_PPkin. 1 hit.
PROSITEiPS00114. PRPP_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and sequencing of human cDNA for phosphoribosyl pyrophosphate synthetase subunit II."
    Iizasa T., Taira M., Shimada H., Ishijima S., Tatibana M.
    FEBS Lett. 244:47-50(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Testis.
  2. "Deduced amino acid sequence from human phosphoribosylpyrophosphate synthetase subunit II cDNA."
    Iizasa T., Taira M., Shimada H., Tatibana M.
    Adv. Exp. Med. Biol. 253A:519-523(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Testis and Uterus.
  4. "Promoter regions of the human X-linked housekeeping genes PRPS1 and PRPS2 encoding phosphoribosylpyrophosphate synthetase subunit I and II isoforms."
    Ishizuka T., Iizasa T., Taira M., Ishijima S., Sonoda T., Shimada H., Nagatake N., Tatibana M.
    Biochim. Biophys. Acta 1130:139-148(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.
    Tissue: Leukocyte.
  5. Cited for: PROTEIN SEQUENCE OF 2-29; 34-49; 85-96; 164-196; 205-214; 244-282 AND 287-318, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Cervix carcinoma, Mammary carcinoma and Osteosarcoma.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPRPS2_HUMAN
AccessioniPrimary (citable) accession number: P11908
Secondary accession number(s): Q0VDH9
, Q0VDI0, Q15245, Q2TAK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: May 27, 2015
This is version 165 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.