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Protein

Ribose-phosphate pyrophosphokinase 2

Gene

PRPS2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.

Catalytic activityi

ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Enzyme regulationi

Activated by magnesium and inorganic phosphate. Competitively or non-competitively inhibited by ADP, 2,3-bisphosphoglyceride or GDP.

Pathwayi: 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I).
Proteins known to be involved in this subpathway in this organism are:
  1. Ribose-phosphate pyrophosphokinase 3 (PRPS1L1), Ribose-phosphate pyrophosphokinase 1 (PRPS1), Ribose-phosphate pyrophosphokinase 2 (PRPS2)
This subpathway is part of the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I), the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi128MagnesiumSequence analysis1
Metal bindingi130MagnesiumSequence analysis1
Binding sitei130ATPBy similarity1
Metal bindingi139MagnesiumSequence analysis1
Metal bindingi143MagnesiumSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi96 – 101ATPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processNucleotide biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS02317-MONOMER.
ReactomeiR-HSA-73843. 5-Phosphoribose 1-diphosphate biosynthesis.
UniPathwayiUPA00087; UER00172.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribose-phosphate pyrophosphokinase 2 (EC:2.7.6.1)
Alternative name(s):
PPRibP
Phosphoribosyl pyrophosphate synthase II
Short name:
PRS-II
Gene namesi
Name:PRPS2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000101911.12.
HGNCiHGNC:9465. PRPS2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi5634.
OpenTargetsiENSG00000101911.
PharmGKBiPA33820.

Polymorphism and mutation databases

BioMutaiPRPS2.
DMDMi125583.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001410742 – 318Ribose-phosphate pyrophosphokinase 2Add BLAST317

Proteomic databases

EPDiP11908.
MaxQBiP11908.
PaxDbiP11908.
PeptideAtlasiP11908.
PRIDEiP11908.

2D gel databases

REPRODUCTION-2DPAGEiIPI00219617.
P11908.

PTM databases

iPTMnetiP11908.
PhosphoSitePlusiP11908.
SwissPalmiP11908.

Expressioni

Gene expression databases

BgeeiENSG00000101911.
CleanExiHS_PRPS2.
ExpressionAtlasiP11908. baseline and differential.
GenevisibleiP11908. HS.

Organism-specific databases

HPAiCAB070417.

Interactioni

Subunit structurei

Homodimer. The active form is probably a hexamer composed of 3 homodimers (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi111617. 23 interactors.
IntActiP11908. 10 interactors.
MINTiMINT-5000929.
STRINGi9606.ENSP00000381504.

Structurei

3D structure databases

ProteinModelPortaliP11908.
SMRiP11908.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni212 – 227Binding of phosphoribosylpyrophosphateSequence analysisAdd BLAST16

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1448. Eukaryota.
COG0462. LUCA.
GeneTreeiENSGT00550000074583.
HOGENOMiHOG000210451.
HOVERGENiHBG001520.
InParanoidiP11908.
KOiK00948.
OMAiPYARMDK.
OrthoDBiEOG091G0ZG8.
PhylomeDBiP11908.
TreeFamiTF106366.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
HAMAPiMF_00583_B. RibP_PPkinase_B. 1 hit.
InterProiView protein in InterPro
IPR000842. PRib_PP_synth_CS.
IPR029099. Pribosyltran_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005946. Rib-P_diPkinase.
PfamiView protein in Pfam
PF14572. Pribosyl_synth. 1 hit.
PF13793. Pribosyltran_N. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01251. ribP_PPkin. 1 hit.
PROSITEiView protein in PROSITE
PS00114. PRPP_SYNTHASE. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P11908-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPNIVLFSGS SHQDLSQRVA DRLGLELGKV VTKKFSNQET SVEIGESVRG
60 70 80 90 100
EDVYIIQSGC GEINDNLMEL LIMINACKIA SSSRVTAVIP CFPYARQDKK
110 120 130 140 150
DKSRAPISAK LVANMLSVAG ADHIITMDLH ASQIQGFFDI PVDNLYAEPA
160 170 180 190 200
VLQWIRENIA EWKNCIIVSP DAGGAKRVTS IADRLNVEFA LIHKERKKAN
210 220 230 240 250
EVDRMVLVGD VKDRVAILVD DMADTCGTIC HAADKLLSAG ATKVYAILTH
260 270 280 290 300
GIFSGPAISR INNAAFEAVV VTNTIPQEDK MKHCTKIQVI DISMILAEAI
310
RRTHNGESVS YLFSHVPL
Length:318
Mass (Da):34,769
Last modified:January 23, 2007 - v2
Checksum:i72A2873408250C31
GO
Isoform 2 (identifier: P11908-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     102-102: K → KVGE

Note: No experimental confirmation available.
Show »
Length:321
Mass (Da):35,054
Checksum:iEDD88B9D33A55A37
GO

Sequence cautioni

The sequence AAH30019 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_027769102K → KVGE in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00971 mRNA. Translation: CAA68785.1.
BC030019 mRNA. Translation: AAH30019.2. Different initiation.
BC040483 mRNA. Translation: AAH40483.3.
BC110875 mRNA. Translation: AAI10876.2.
BC119662 mRNA. Translation: AAI19663.1.
BC119663 mRNA. Translation: AAI19664.1.
D28134 Genomic DNA. Translation: BAA05676.1.
CCDSiCCDS14150.1. [P11908-1]
CCDS43918.1. [P11908-2]
PIRiS02778. KIHUR2.
RefSeqiNP_001034180.1. NM_001039091.2. [P11908-2]
NP_002756.1. NM_002765.4. [P11908-1]
UniGeneiHs.654581.

Genome annotation databases

EnsembliENST00000380668; ENSP00000370043; ENSG00000101911. [P11908-1]
ENST00000398491; ENSP00000381504; ENSG00000101911. [P11908-2]
GeneIDi5634.
KEGGihsa:5634.
UCSCiuc004cva.4. human. [P11908-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPRPS2_HUMAN
AccessioniPrimary (citable) accession number: P11908
Secondary accession number(s): Q0VDH9
, Q0VDI0, Q15245, Q2TAK7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: September 27, 2017
This is version 188 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families