ID   T421_ECOLX              Reviewed;         371 AA.
AC   P11901;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   24-JAN-2024, entry version 79.
DE   RecName: Full=Transposase for insertion sequence element IS421;
OS   Escherichia coli.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=B, and C;
RX   PubMed=2542093; DOI=10.1016/0014-5793(89)80007-9;
RA   Sato S., Nakada Y., Shiratsuchi A.;
RT   "IS421, a new insertion sequence in Escherichia coli.";
RL   FEBS Lett. 249:21-26(1989).
CC   -!- FUNCTION: Involved in the transposition of the insertion sequence
CC       IS421.
CC   -!- SIMILARITY: Belongs to the transposase 11 family. {ECO:0000305}.
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DR   EMBL; Y07501; CAA68802.1; -; Genomic_DNA.
DR   PIR; S04880; S04880.
DR   AlphaFoldDB; P11901; -.
DR   eggNOG; COG3385; Bacteria.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004803; F:transposase activity; IEA:InterPro.
DR   GO; GO:0006313; P:DNA transposition; IEA:InterPro.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR047952; Transpos_IS4.
DR   InterPro; IPR002559; Transposase_11.
DR   NCBIfam; NF033592; transpos_IS4_1; 1.
DR   PANTHER; PTHR33258; TRANSPOSASE INSL FOR INSERTION SEQUENCE ELEMENT IS186A-RELATED; 1.
DR   PANTHER; PTHR33258:SF1; TRANSPOSASE INSL FOR INSERTION SEQUENCE ELEMENT IS186A-RELATED; 1.
DR   Pfam; PF01609; DDE_Tnp_1; 1.
DR   SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE   3: Inferred from homology;
KW   DNA recombination; DNA-binding; Transposable element; Transposition.
FT   CHAIN           1..371
FT                   /note="Transposase for insertion sequence element IS421"
FT                   /id="PRO_0000173295"
SQ   SEQUENCE   371 AA;  40996 MW;  2BD731641192DF38 CRC64;
     MNYSHDNWSA ILAHIGKPEE LDTSARNAGA LTRRREIRDA ATLLRLGLAY GPGGMSSLRE
     VTAWAQLHDV ATLSDVALLK RLRNAADWFG ILAAQTLAVR AAVTGCTSGK RLRLVDGTAI
     SAPGGGSAEW RLHMGYDPHT CQFTDFELTD SRDAERLDRF AQTADEIRIA DRGFGSRPEC
     IRSLAFGEAD YIVRVHWRGL RWLTAEGMRF DMMGFLRGLD CGKNGETTVM IGNSGNKKAG
     APFPARLIAV SLPPEKALIS KTRLLSENRR KGRVVQAETL EAAGHVLLLT SLPEDEYSAE
     QVADCYRLRW QIELAFKRLK SLLHLDALRA KEPELAKAWI FANLLAAFLI DDIIQPSLDF
     PPRSAGSEKK N
//