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Protein

Aldehyde dehydrogenase, mitochondrial

Gene

Aldh2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

An aldehyde + NAD+ + H2O = a carboxylate + NADH.

Pathwayi: ethanol degradation

This protein is involved in step 2 of the subpathway that synthesizes acetate from ethanol.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Aldehyde dehydrogenase X, mitochondrial (Aldh1b1), Aldehyde dehydrogenase, mitochondrial (Aldh2), Aldehyde dehydrogenase, cytosolic 1 (Aldh1a7), Aldehyde dehydrogenase family 3 member B1 (Aldh3b1)
This subpathway is part of the pathway ethanol degradation, which is itself part of Alcohol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetate from ethanol, the pathway ethanol degradation and in Alcohol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei188Transition state stabilizerBy similarity1
Active sitei287Proton acceptor1
Active sitei321Nucleophile1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi264 – 269NADBy similarity6

GO - Molecular functioni

  • aldehyde dehydrogenase (NAD) activity Source: CACAO
  • identical protein binding Source: RGD
  • NADH binding Source: RGD

GO - Biological processi

  • cellular response to fatty acid Source: RGD
  • cellular response to hormone stimulus Source: RGD
  • ethanol catabolic process Source: UniProtKB-UniPathway
  • liver development Source: RGD
  • negative regulation of apoptotic process Source: RGD
  • response to estradiol Source: RGD
  • response to hyperoxia Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to nicotine Source: RGD
  • response to progesterone Source: RGD
  • response to testosterone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.2.1.3. 5301.
1.2.1.5. 5301.
SABIO-RKP11884.
UniPathwayiUPA00780; UER00768.

Names & Taxonomyi

Protein namesi
Recommended name:
Aldehyde dehydrogenase, mitochondrial (EC:1.2.1.3)
Alternative name(s):
ALDH class 2
ALDH-E2
ALDH1
Gene namesi
Name:Aldh2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69219. Aldh2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2812.
GuidetoPHARMACOLOGYi2595.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 19MitochondrionAdd BLAST19
ChainiPRO_000000717020 – 519Aldehyde dehydrogenase, mitochondrialAdd BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54N6-acetyllysineBy similarity1
Modified residuei75N6-acetyllysineBy similarity1
Modified residuei80N6-acetyllysineBy similarity1
Modified residuei161N6-acetyllysineBy similarity1
Modified residuei370N6-acetyllysineBy similarity1
Modified residuei377N6-acetyllysineBy similarity1
Modified residuei385N6-acetyllysineBy similarity1
Modified residuei409N6-acetyllysineBy similarity1
Modified residuei428N6-acetyllysineBy similarity1
Modified residuei430N6-acetyllysineBy similarity1
Modified residuei443N6-acetyllysineBy similarity1
Modified residuei453N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP11884.
PRIDEiP11884.

2D gel databases

World-2DPAGE0004:P11884.

PTM databases

iPTMnetiP11884.
PhosphoSitePlusiP11884.

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
TIM50Q027763EBI-916402,EBI-30302From a different organism.

GO - Molecular functioni

  • identical protein binding Source: RGD

Protein-protein interaction databases

BioGridi248173. 1 interactor.
IntActiP11884. 3 interactors.
MINTiMINT-4569918.
STRINGi10116.ENSRNOP00000001816.

Chemistry databases

BindingDBiP11884.

Structurei

Secondary structure

1519
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 15Combined sources3
Helixi16 – 18Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OM2NMR-B12-20[»]
2V1SX-ray2.05H/I/J/K/L/M/N12-24[»]
2V1TX-ray1.92C/D12-22[»]
3AWRX-ray2.00C/D12-20[»]
3AX2X-ray1.90B/D/F/H12-20[»]
3AX3X-ray2.10B/D/F/H12-20[»]
3AX5X-ray2.20B/D12-20[»]
5AZ8X-ray1.70B12-24[»]
ProteinModelPortaliP11884.
SMRiP11884.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11884.

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2450. Eukaryota.
COG1012. LUCA.
HOGENOMiHOG000271505.
HOVERGENiHBG000097.
InParanoidiP11884.
KOiK00128.
PhylomeDBiP11884.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11884-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRAALSTAR RGPRLSRLLS AAATSAVPAP NQQPEVFCNQ IFINNEWHDA
60 70 80 90 100
VSKKTFPTVN PSTGEVICQV AEGNKEDVDK AVKAAQAAFQ LGSPWRRMDA
110 120 130 140 150
SDRGRLLYRL ADLIERDRTY LAALETLDNG KPYVISYLVD LDMVLKCLRY
160 170 180 190 200
YAGWADKYHG KTIPIDGDFF SYTRHEPVGV CGQIIPWNFP LLMQAWKLGP
210 220 230 240 250
ALATGNVVVM KVAEQTPLTA LYVANLIKEA GFPPGVVNIV PGFGPTAGAA
260 270 280 290 300
IASHEDVDKV AFTGSTEVGH LIQVAAGSSN LKRVTLELGG KSPNIIMSDA
310 320 330 340 350
DMDWAVEQAH FALFFNQGQC CCAGSRTFVQ EDVYDEFVER SVARAKSRVV
360 370 380 390 400
GNPFDSRTEQ GPQVDETQFK KILGYIKSGQ QEGAKLLCGG GAAADRGYFI
410 420 430 440 450
QPTVFGDVKD GMTIAKEEIF GPVMQILKFK TIEEVVGRAN NSKYGLAAAV
460 470 480 490 500
FTKDLDKANY LSQALQAGTV WINCYDVFGA QSPFGGYKMS GSGRELGEYG
510
LQAYTEVKTV TVKVPQKNS
Length:519
Mass (Da):56,488
Last modified:October 1, 1989 - v1
Checksum:i75C748202F1333E5
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti86Q → R in allele Aldh2*2 and allele Aldh2*3; in strains UChA and UChB. 1 Publication1
Natural varianti498E → K in allele Aldh2*3; in strain UChB. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14977 mRNA. Translation: CAA33101.1.
BC062081 mRNA. Translation: AAH62081.1.
M19030 mRNA. Translation: AAA40719.1.
AY566467 mRNA. Translation: AAS75813.1.
AY566468 mRNA. Translation: AAS75814.1.
AY566469 mRNA. Translation: AAS75815.1.
AF529165 mRNA. Translation: AAM94394.2.
AY034137 Genomic DNA. Translation: AAK57732.1.
PIRiS03564.
RefSeqiNP_115792.1. NM_032416.1.
UniGeneiRn.101781.

Genome annotation databases

GeneIDi29539.
KEGGirno:29539.
UCSCiRGD:69219. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14977 mRNA. Translation: CAA33101.1.
BC062081 mRNA. Translation: AAH62081.1.
M19030 mRNA. Translation: AAA40719.1.
AY566467 mRNA. Translation: AAS75813.1.
AY566468 mRNA. Translation: AAS75814.1.
AY566469 mRNA. Translation: AAS75815.1.
AF529165 mRNA. Translation: AAM94394.2.
AY034137 Genomic DNA. Translation: AAK57732.1.
PIRiS03564.
RefSeqiNP_115792.1. NM_032416.1.
UniGeneiRn.101781.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OM2NMR-B12-20[»]
2V1SX-ray2.05H/I/J/K/L/M/N12-24[»]
2V1TX-ray1.92C/D12-22[»]
3AWRX-ray2.00C/D12-20[»]
3AX2X-ray1.90B/D/F/H12-20[»]
3AX3X-ray2.10B/D/F/H12-20[»]
3AX5X-ray2.20B/D12-20[»]
5AZ8X-ray1.70B12-24[»]
ProteinModelPortaliP11884.
SMRiP11884.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248173. 1 interactor.
IntActiP11884. 3 interactors.
MINTiMINT-4569918.
STRINGi10116.ENSRNOP00000001816.

Chemistry databases

BindingDBiP11884.
ChEMBLiCHEMBL2812.
GuidetoPHARMACOLOGYi2595.

PTM databases

iPTMnetiP11884.
PhosphoSitePlusiP11884.

2D gel databases

World-2DPAGE0004:P11884.

Proteomic databases

PaxDbiP11884.
PRIDEiP11884.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29539.
KEGGirno:29539.
UCSCiRGD:69219. rat.

Organism-specific databases

CTDi217.
RGDi69219. Aldh2.

Phylogenomic databases

eggNOGiKOG2450. Eukaryota.
COG1012. LUCA.
HOGENOMiHOG000271505.
HOVERGENiHBG000097.
InParanoidiP11884.
KOiK00128.
PhylomeDBiP11884.

Enzyme and pathway databases

UniPathwayiUPA00780; UER00768.
BRENDAi1.2.1.3. 5301.
1.2.1.5. 5301.
SABIO-RKP11884.

Miscellaneous databases

EvolutionaryTraceiP11884.
PROiP11884.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALDH2_RAT
AccessioniPrimary (citable) accession number: P11884
Secondary accession number(s): Q6Q288
, Q6Q289, Q6Q290, Q8K3V8, Q91ZD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 30, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.