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P11884

- ALDH2_RAT

UniProt

P11884 - ALDH2_RAT

Protein

Aldehyde dehydrogenase, mitochondrial

Gene

Aldh2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 1 (01 Oct 1989)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    An aldehyde + NAD+ + H2O = a carboxylate + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei188 – 1881Transition state stabilizer
    Active sitei287 – 2871Proton acceptor
    Active sitei321 – 3211Nucleophile

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi264 – 2696NADBy similarity

    GO - Molecular functioni

    1. aldehyde dehydrogenase (NAD) activity Source: RGD
    2. identical protein binding Source: RGD
    3. NADH binding Source: RGD

    GO - Biological processi

    1. cellular response to fatty acid Source: RGD
    2. cellular response to hormone stimulus Source: RGD
    3. ethanol catabolic process Source: UniProtKB-UniPathway
    4. liver development Source: RGD
    5. negative regulation of apoptotic process Source: RGD
    6. response to estradiol Source: RGD
    7. response to hyperoxia Source: RGD
    8. response to lipopolysaccharide Source: RGD
    9. response to nicotine Source: RGD
    10. response to progesterone Source: RGD
    11. response to testosterone Source: RGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    SABIO-RKP11884.
    UniPathwayiUPA00780; UER00768.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aldehyde dehydrogenase, mitochondrial (EC:1.2.1.3)
    Alternative name(s):
    ALDH class 2
    ALDH-E2
    ALDH1
    Gene namesi
    Name:Aldh2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi69219. Aldh2.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell
    2. mitochondrion Source: RGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1919MitochondrionAdd
    BLAST
    Chaini20 – 519500Aldehyde dehydrogenase, mitochondrialPRO_0000007170Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei20 – 201N-acetylserineCurated
    Modified residuei54 – 541N6-acetyllysineBy similarity
    Modified residuei75 – 751N6-acetyllysineBy similarity
    Modified residuei80 – 801N6-acetyllysineBy similarity
    Modified residuei161 – 1611N6-acetyllysineBy similarity
    Modified residuei370 – 3701N6-acetyllysineBy similarity
    Modified residuei377 – 3771N6-acetyllysineBy similarity
    Modified residuei385 – 3851N6-acetyllysineBy similarity
    Modified residuei409 – 4091N6-acetyllysineBy similarity
    Modified residuei428 – 4281N6-acetyllysineBy similarity
    Modified residuei430 – 4301N6-acetyllysineBy similarity
    Modified residuei443 – 4431N6-acetyllysineBy similarity
    Modified residuei453 – 4531N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP11884.
    PRIDEiP11884.

    2D gel databases

    World-2DPAGE0004:P11884.

    PTM databases

    PhosphoSiteiP11884.

    Expressioni

    Gene expression databases

    GenevestigatoriP11884.

    Interactioni

    Subunit structurei

    Homotetramer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TIM50Q027763EBI-916402,EBI-30302From a different organism.

    Protein-protein interaction databases

    IntActiP11884. 3 interactions.
    MINTiMINT-4569918.

    Structurei

    Secondary structure

    1
    519
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi15 – 206

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OM2NMR-B12-20[»]
    2V1SX-ray2.05H/I/J/K/L/M/N12-24[»]
    2V1TX-ray1.92C/D12-22[»]
    3AWRX-ray2.00C/D12-20[»]
    3AX2X-ray1.90B/D/F/H12-20[»]
    3AX3X-ray2.10B/D/F/H12-20[»]
    3AX5X-ray2.20B/D12-20[»]
    ProteinModelPortaliP11884.
    SMRiP11884. Positions 26-519.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP11884.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldehyde dehydrogenase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1012.
    HOGENOMiHOG000271505.
    HOVERGENiHBG000097.
    InParanoidiP11884.
    KOiK00128.
    PhylomeDBiP11884.

    Family and domain databases

    Gene3Di3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProiIPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR029510. Ald_DH_CS_GLU.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    [Graphical view]
    PfamiPF00171. Aldedh. 1 hit.
    [Graphical view]
    SUPFAMiSSF53720. SSF53720. 1 hit.
    PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P11884-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRAALSTAR RGPRLSRLLS AAATSAVPAP NQQPEVFCNQ IFINNEWHDA    50
    VSKKTFPTVN PSTGEVICQV AEGNKEDVDK AVKAAQAAFQ LGSPWRRMDA 100
    SDRGRLLYRL ADLIERDRTY LAALETLDNG KPYVISYLVD LDMVLKCLRY 150
    YAGWADKYHG KTIPIDGDFF SYTRHEPVGV CGQIIPWNFP LLMQAWKLGP 200
    ALATGNVVVM KVAEQTPLTA LYVANLIKEA GFPPGVVNIV PGFGPTAGAA 250
    IASHEDVDKV AFTGSTEVGH LIQVAAGSSN LKRVTLELGG KSPNIIMSDA 300
    DMDWAVEQAH FALFFNQGQC CCAGSRTFVQ EDVYDEFVER SVARAKSRVV 350
    GNPFDSRTEQ GPQVDETQFK KILGYIKSGQ QEGAKLLCGG GAAADRGYFI 400
    QPTVFGDVKD GMTIAKEEIF GPVMQILKFK TIEEVVGRAN NSKYGLAAAV 450
    FTKDLDKANY LSQALQAGTV WINCYDVFGA QSPFGGYKMS GSGRELGEYG 500
    LQAYTEVKTV TVKVPQKNS 519
    Length:519
    Mass (Da):56,488
    Last modified:October 1, 1989 - v1
    Checksum:i75C748202F1333E5
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti86 – 861Q → R in allele Aldh2*2 and allele Aldh2*3; in strains UChA and UChB. 1 Publication
    Natural varianti498 – 4981E → K in allele Aldh2*3; in strain UChB. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14977 mRNA. Translation: CAA33101.1.
    BC062081 mRNA. Translation: AAH62081.1.
    M19030 mRNA. Translation: AAA40719.1.
    AY566467 mRNA. Translation: AAS75813.1.
    AY566468 mRNA. Translation: AAS75814.1.
    AY566469 mRNA. Translation: AAS75815.1.
    AF529165 mRNA. Translation: AAM94394.2.
    AY034137 Genomic DNA. Translation: AAK57732.1.
    PIRiS03564.
    RefSeqiNP_115792.1. NM_032416.1.
    UniGeneiRn.101781.

    Genome annotation databases

    GeneIDi29539.
    KEGGirno:29539.
    UCSCiRGD:69219. rat.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14977 mRNA. Translation: CAA33101.1 .
    BC062081 mRNA. Translation: AAH62081.1 .
    M19030 mRNA. Translation: AAA40719.1 .
    AY566467 mRNA. Translation: AAS75813.1 .
    AY566468 mRNA. Translation: AAS75814.1 .
    AY566469 mRNA. Translation: AAS75815.1 .
    AF529165 mRNA. Translation: AAM94394.2 .
    AY034137 Genomic DNA. Translation: AAK57732.1 .
    PIRi S03564.
    RefSeqi NP_115792.1. NM_032416.1.
    UniGenei Rn.101781.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OM2 NMR - B 12-20 [» ]
    2V1S X-ray 2.05 H/I/J/K/L/M/N 12-24 [» ]
    2V1T X-ray 1.92 C/D 12-22 [» ]
    3AWR X-ray 2.00 C/D 12-20 [» ]
    3AX2 X-ray 1.90 B/D/F/H 12-20 [» ]
    3AX3 X-ray 2.10 B/D/F/H 12-20 [» ]
    3AX5 X-ray 2.20 B/D 12-20 [» ]
    ProteinModelPortali P11884.
    SMRi P11884. Positions 26-519.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P11884. 3 interactions.
    MINTi MINT-4569918.

    Chemistry

    BindingDBi P11884.
    ChEMBLi CHEMBL2812.
    GuidetoPHARMACOLOGYi 2595.

    PTM databases

    PhosphoSitei P11884.

    2D gel databases

    World-2DPAGE 0004:P11884.

    Proteomic databases

    PaxDbi P11884.
    PRIDEi P11884.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 29539.
    KEGGi rno:29539.
    UCSCi RGD:69219. rat.

    Organism-specific databases

    CTDi 217.
    RGDi 69219. Aldh2.

    Phylogenomic databases

    eggNOGi COG1012.
    HOGENOMi HOG000271505.
    HOVERGENi HBG000097.
    InParanoidi P11884.
    KOi K00128.
    PhylomeDBi P11884.

    Enzyme and pathway databases

    UniPathwayi UPA00780 ; UER00768 .
    SABIO-RK P11884.

    Miscellaneous databases

    EvolutionaryTracei P11884.
    NextBioi 609531.

    Gene expression databases

    Genevestigatori P11884.

    Family and domain databases

    Gene3Di 3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProi IPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR029510. Ald_DH_CS_GLU.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    [Graphical view ]
    Pfami PF00171. Aldedh. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53720. SSF53720. 1 hit.
    PROSITEi PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structures of rat and bovine liver mitochondrial aldehyde dehydrogenases deduced from cDNA sequences."
      Farres J., Guan K.-L., Weiner H.
      Eur. J. Biochem. 180:67-74(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.
    3. "Sequence of the signal peptide for rat liver mitochondrial aldehyde dehydrogenase."
      Farres J., Guan K.-L., Weiner H.
      Biochem. Biophys. Res. Commun. 150:1083-1087(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-29.
      Tissue: Liver.
    4. "Purification and characterization of catalytically active precursor of rat liver mitochondrial aldehyde dehydrogenase expressed in Escherichia coli."
      Jeng J., Weiner H.
      Arch. Biochem. Biophys. 289:214-222(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-19.
      Tissue: Liver.
    5. "Mutations in mitochondrial aldehyde dehydrogenase (ALDH2) change cofactor affinity and segregate with voluntary alcohol consumption in rats."
      Sapag A., Tampier L., Valle-Prieto A., Quintanilla M.E., Moncada C., Israel Y.
      Pharmacogenetics 13:509-515(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-519, VARIANTS ARG-86 AND LYS-498.
      Strain: UChA, UChB and Wistar.
      Tissue: Liver.
    6. "Genomic structure and sequence of the mitochondrial aldehyde dehydrogenase gene (ALDH2) of the Lewis rat."
      Sapag A., Urra S., Gonzalez-Jara F., Moncada C., Israel Y.
      Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-519.
      Strain: Lewis.
    7. "Genomic structure and sequence of the mitochondrial aldehyde dehydrogenase gene of the Lewis rat."
      Sapag A., Urra S., Gonzalez-Jara F., Moncada C., Israel Y.
      Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 77-519.
      Strain: Lewis.
      Tissue: Liver.
    8. Lubec G., Afjehi-Sadat L., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 162-174; 198-228; 260-340; 327-340; 358-370; 397-409 AND 495-508, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus and Spinal cord.
    9. "A mitochondrial protein fraction catalyzing transport of the K+ analog T1+."
      Diwan J.J., Paliwal R., Kaftan E., Bawa R.
      FEBS Lett. 273:215-218(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 327-340.
    10. "Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20."
      Abe Y., Shodai T., Muto T., Mihara K., Torii H., Nishikawa S., Endo T., Kohda D.
      Cell 100:551-560(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 12-22.

    Entry informationi

    Entry nameiALDH2_RAT
    AccessioniPrimary (citable) accession number: P11884
    Secondary accession number(s): Q6Q288
    , Q6Q289, Q6Q290, Q8K3V8, Q91ZD7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 140 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3