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Protein

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase

Gene

murF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.UniRule annotation1 Publication

Catalytic activityi

ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.UniRule annotation1 Publication

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi107 – 1137ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan biosynthetic process Source: EcoCyc
  • regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:UDP-NACMURALGLDAPAALIG-MONOMER.
ECOL316407:JW0084-MONOMER.
MetaCyc:UDP-NACMURALGLDAPAALIG-MONOMER.
BRENDAi6.3.2.10. 2026.
6.3.2.8. 2026.
SABIO-RKP11880.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseUniRule annotationCurated (EC:6.3.2.10UniRule annotation1 Publication)
Alternative name(s):
D-alanyl-D-alanine-adding enzyme1 PublicationUniRule annotation
UDP-MurNAc-pentapeptide synthetase1 Publication
Gene namesi
Name:murF1 PublicationUniRule annotation
Synonyms:mra
Ordered Locus Names:b0086, JW0084
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10622. murF.

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2540.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 452452UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligasePRO_0000101698Add
BLAST

Proteomic databases

EPDiP11880.
PaxDbiP11880.
PRIDEiP11880.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4261642. 416 interactions.
DIPiDIP-10281N.
IntActiP11880. 11 interactions.
MINTiMINT-1227223.
STRINGi511145.b0086.

Chemistry

BindingDBiP11880.

Structurei

Secondary structure

1
452
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 127Combined sources
Beta strandi16 – 194Combined sources
Beta strandi23 – 253Combined sources
Beta strandi27 – 293Combined sources
Helixi31 – 333Combined sources
Beta strandi39 – 413Combined sources
Turni50 – 534Combined sources
Helixi54 – 596Combined sources
Beta strandi64 – 696Combined sources
Beta strandi77 – 793Combined sources
Helixi83 – 9715Combined sources
Beta strandi101 – 1066Combined sources
Helixi111 – 12212Combined sources
Turni123 – 1253Combined sources
Beta strandi128 – 1303Combined sources
Helixi140 – 1456Combined sources
Beta strandi153 – 1586Combined sources
Beta strandi162 – 1654Combined sources
Helixi166 – 1749Combined sources
Beta strandi177 – 1815Combined sources
Helixi195 – 2039Combined sources
Helixi204 – 2085Combined sources
Beta strandi214 – 2185Combined sources
Helixi224 – 2318Combined sources
Beta strandi234 – 2396Combined sources
Beta strandi247 – 2559Combined sources
Beta strandi257 – 26610Combined sources
Beta strandi269 – 2757Combined sources
Beta strandi277 – 2793Combined sources
Helixi281 – 29515Combined sources
Helixi300 – 3078Combined sources
Beta strandi316 – 3238Combined sources
Beta strandi326 – 3305Combined sources
Helixi337 – 34913Combined sources
Beta strandi350 – 3589Combined sources
Helixi367 – 38216Combined sources
Beta strandi385 – 3939Combined sources
Helixi395 – 4006Combined sources
Beta strandi405 – 4095Combined sources
Helixi410 – 42314Combined sources
Beta strandi425 – 4328Combined sources
Helixi435 – 4373Combined sources
Helixi440 – 4467Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GG4X-ray2.30A/B1-452[»]
ProteinModelPortaliP11880.
SMRiP11880. Positions 1-447.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11880.

Family & Domainsi

Sequence similaritiesi

Belongs to the MurCDEF family. MurF subfamily.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4108EQQ. Bacteria.
COG0770. LUCA.
HOGENOMiHOG000268120.
InParanoidiP11880.
KOiK01929.
OMAiYTALHMP.
OrthoDBiEOG6PKFCR.
PhylomeDBiP11880.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_02019. MurF.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005863. UDP-N-AcMur_synth.
[Graphical view]
PANTHERiPTHR23135:SF3. PTHR23135:SF3. 1 hit.
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01143. murF. 1 hit.

Sequencei

Sequence statusi: Complete.

P11880-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISVTLSQLT DILNGELQGA DITLDAVTTD TRKLTPGCLF VALKGERFDA
60 70 80 90 100
HDFADQAKAG GAGALLVSRP LDIDLPQLIV KDTRLAFGEL AAWVRQQVPA
110 120 130 140 150
RVVALTGSSG KTSVKEMTAA ILSQCGNTLY TAGNLNNDIG VPMTLLRLTP
160 170 180 190 200
EYDYAVIELG ANHQGEIAWT VSLTRPEAAL VNNLAAAHLE GFGSLAGVAK
210 220 230 240 250
AKGEIFSGLP ENGIAIMNAD NNDWLNWQSV IGSRKVWRFS PNAANSDFTA
260 270 280 290 300
TNIHVTSHGT EFTLQTPTGS VDVLLPLPGR HNIANALAAA ALSMSVGATL
310 320 330 340 350
DAIKAGLANL KAVPGRLFPI QLAENQLLLD DSYNANVGSM TAAVQVLAEM
360 370 380 390 400
PGYRVLVVGD MAELGAESEA CHVQVGEAAK AAGIDRVLSV GKQSHAISTA
410 420 430 440 450
SGVGEHFADK TALITRLKLL IAEQQVITIL VKGSRSAAME EVVRALQENG

TC
Length:452
Mass (Da):47,447
Last modified:November 1, 1997 - v2
Checksum:iB46E2E57BDBBC572
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti61 – 611G → A (PubMed:2668880).Curated
Sequence conflicti61 – 611G → A (PubMed:1630901).Curated
Sequence conflicti178 – 1781A → R (PubMed:2668880).Curated
Sequence conflicti178 – 1781A → R (PubMed:1630901).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti288 – 2881A → T in murF2; temperature-sensitive mutant with low activity. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15432 Genomic DNA. Translation: CAA33473.1.
X55034 Genomic DNA. Translation: CAA38863.1.
U67891 Genomic DNA. Translation: AAC44657.1.
U00096 Genomic DNA. Translation: AAC73197.1.
AP009048 Genomic DNA. Translation: BAB96654.2.
U67893 Genomic DNA. Translation: AAB60788.1.
PIRiF64730.
RefSeqiNP_414628.1. NC_000913.3.
WP_000626685.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73197; AAC73197; b0086.
BAB96654; BAB96654; BAB96654.
GeneIDi944813.
KEGGiecj:JW0084.
eco:b0086.
PATRICi32115277. VBIEscCol129921_0090.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15432 Genomic DNA. Translation: CAA33473.1.
X55034 Genomic DNA. Translation: CAA38863.1.
U67891 Genomic DNA. Translation: AAC44657.1.
U00096 Genomic DNA. Translation: AAC73197.1.
AP009048 Genomic DNA. Translation: BAB96654.2.
U67893 Genomic DNA. Translation: AAB60788.1.
PIRiF64730.
RefSeqiNP_414628.1. NC_000913.3.
WP_000626685.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GG4X-ray2.30A/B1-452[»]
ProteinModelPortaliP11880.
SMRiP11880. Positions 1-447.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261642. 416 interactions.
DIPiDIP-10281N.
IntActiP11880. 11 interactions.
MINTiMINT-1227223.
STRINGi511145.b0086.

Chemistry

BindingDBiP11880.
ChEMBLiCHEMBL2540.

Proteomic databases

EPDiP11880.
PaxDbiP11880.
PRIDEiP11880.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73197; AAC73197; b0086.
BAB96654; BAB96654; BAB96654.
GeneIDi944813.
KEGGiecj:JW0084.
eco:b0086.
PATRICi32115277. VBIEscCol129921_0090.

Organism-specific databases

EchoBASEiEB0617.
EcoGeneiEG10622. murF.

Phylogenomic databases

eggNOGiENOG4108EQQ. Bacteria.
COG0770. LUCA.
HOGENOMiHOG000268120.
InParanoidiP11880.
KOiK01929.
OMAiYTALHMP.
OrthoDBiEOG6PKFCR.
PhylomeDBiP11880.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciEcoCyc:UDP-NACMURALGLDAPAALIG-MONOMER.
ECOL316407:JW0084-MONOMER.
MetaCyc:UDP-NACMURALGLDAPAALIG-MONOMER.
BRENDAi6.3.2.10. 2026.
6.3.2.8. 2026.
SABIO-RKP11880.

Miscellaneous databases

EvolutionaryTraceiP11880.
PROiP11880.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_02019. MurF.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005863. UDP-N-AcMur_synth.
[Graphical view]
PANTHERiPTHR23135:SF3. PTHR23135:SF3. 1 hit.
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01143. murF. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the murF gene encoding the UDP-MurNAc-pentapeptide synthetase of Escherichia coli."
    Parquet C., Flouret B., Mengin-Lecreulx D., van Heijenoort J.
    Nucleic Acids Res. 17:5379-5379(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Kinetic mechanism of the Escherichia coli UDPMurNAc-tripeptide D-alanyl-D-alanine-adding enzyme: use of a glutathione S-transferase fusion."
    Anderson M.S., Eveland S.S., Onishi H.R., Pompliano D.L.
    Biochemistry 35:16264-16269(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / MB2884.
  3. "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
    Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
    Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 61 AND 178.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily."
    Eveland S.S., Pompliano D.L., Anderson M.S.
    Biochemistry 36:6223-6229(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTANT MURF2.
    Strain: CGSC 5990.
  7. "Purification and characterization of the D-alanyl-D-alanine-adding enzyme from Escherichia coli."
    Duncan K., van Heijenoort J., Walsh C.T.
    Biochemistry 29:2379-2386(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-15, FUNCTION, CATALYTIC ACTIVITY, PATHWAY, SUBUNIT, SUBCELLULAR LOCATION.
  8. "Crystal structure of Escherichia coli UDPMurNAc-tripeptide D-alanyl-D-alanine-adding enzyme (MurF) at 2.3-A resolution."
    Yan Y., Munshi S., Leiting B., Anderson M.S., Chrzas J., Chen Z.
    J. Mol. Biol. 304:435-445(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).

Entry informationi

Entry nameiMURF_ECOLI
AccessioniPrimary (citable) accession number: P11880
Secondary accession number(s): O07100, P77636
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: November 1, 1997
Last modified: April 13, 2016
This is version 162 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.