Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase

Gene

murF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.UniRule annotation1 Publication

Catalytic activityi

ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.UniRule annotation1 Publication

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi107 – 113ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan biosynthetic process Source: EcoCyc
  • regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:UDP-NACMURALGLDAPAALIG-MONOMER.
ECOL316407:JW0084-MONOMER.
MetaCyc:UDP-NACMURALGLDAPAALIG-MONOMER.
BRENDAi6.3.2.10. 2026.
6.3.2.8. 2026.
SABIO-RKP11880.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseUniRule annotationCurated (EC:6.3.2.10UniRule annotation1 Publication)
Alternative name(s):
D-alanyl-D-alanine-adding enzyme1 PublicationUniRule annotation
UDP-MurNAc-pentapeptide synthetase1 Publication
Gene namesi
Name:murF1 PublicationUniRule annotation
Synonyms:mra
Ordered Locus Names:b0086, JW0084
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10622. murF.

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2540.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001016981 – 452UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseAdd BLAST452

Proteomic databases

EPDiP11880.
PaxDbiP11880.
PRIDEiP11880.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4261642. 416 interactors.
DIPiDIP-10281N.
IntActiP11880. 11 interactors.
MINTiMINT-1227223.
STRINGi511145.b0086.

Chemistry databases

BindingDBiP11880.

Structurei

Secondary structure

1452
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 12Combined sources7
Beta strandi16 – 19Combined sources4
Beta strandi23 – 25Combined sources3
Beta strandi27 – 29Combined sources3
Helixi31 – 33Combined sources3
Beta strandi39 – 41Combined sources3
Turni50 – 53Combined sources4
Helixi54 – 59Combined sources6
Beta strandi64 – 69Combined sources6
Beta strandi77 – 79Combined sources3
Helixi83 – 97Combined sources15
Beta strandi101 – 106Combined sources6
Helixi111 – 122Combined sources12
Turni123 – 125Combined sources3
Beta strandi128 – 130Combined sources3
Helixi140 – 145Combined sources6
Beta strandi153 – 158Combined sources6
Beta strandi162 – 165Combined sources4
Helixi166 – 174Combined sources9
Beta strandi177 – 181Combined sources5
Helixi195 – 203Combined sources9
Helixi204 – 208Combined sources5
Beta strandi214 – 218Combined sources5
Helixi224 – 231Combined sources8
Beta strandi234 – 239Combined sources6
Beta strandi247 – 255Combined sources9
Beta strandi257 – 266Combined sources10
Beta strandi269 – 275Combined sources7
Beta strandi277 – 279Combined sources3
Helixi281 – 295Combined sources15
Helixi300 – 307Combined sources8
Beta strandi316 – 323Combined sources8
Beta strandi326 – 330Combined sources5
Helixi337 – 349Combined sources13
Beta strandi350 – 358Combined sources9
Helixi367 – 382Combined sources16
Beta strandi385 – 393Combined sources9
Helixi395 – 400Combined sources6
Beta strandi405 – 409Combined sources5
Helixi410 – 423Combined sources14
Beta strandi425 – 432Combined sources8
Helixi435 – 437Combined sources3
Helixi440 – 446Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GG4X-ray2.30A/B1-452[»]
ProteinModelPortaliP11880.
SMRiP11880.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11880.

Family & Domainsi

Sequence similaritiesi

Belongs to the MurCDEF family. MurF subfamily.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4108EQQ. Bacteria.
COG0770. LUCA.
HOGENOMiHOG000268120.
InParanoidiP11880.
KOiK01929.
OMAiDYAVIEM.
PhylomeDBiP11880.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_02019. MurF. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005863. UDP-N-AcMur_synth.
[Graphical view]
PANTHERiPTHR23135:SF3. PTHR23135:SF3. 1 hit.
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01143. murF. 1 hit.

Sequencei

Sequence statusi: Complete.

P11880-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISVTLSQLT DILNGELQGA DITLDAVTTD TRKLTPGCLF VALKGERFDA
60 70 80 90 100
HDFADQAKAG GAGALLVSRP LDIDLPQLIV KDTRLAFGEL AAWVRQQVPA
110 120 130 140 150
RVVALTGSSG KTSVKEMTAA ILSQCGNTLY TAGNLNNDIG VPMTLLRLTP
160 170 180 190 200
EYDYAVIELG ANHQGEIAWT VSLTRPEAAL VNNLAAAHLE GFGSLAGVAK
210 220 230 240 250
AKGEIFSGLP ENGIAIMNAD NNDWLNWQSV IGSRKVWRFS PNAANSDFTA
260 270 280 290 300
TNIHVTSHGT EFTLQTPTGS VDVLLPLPGR HNIANALAAA ALSMSVGATL
310 320 330 340 350
DAIKAGLANL KAVPGRLFPI QLAENQLLLD DSYNANVGSM TAAVQVLAEM
360 370 380 390 400
PGYRVLVVGD MAELGAESEA CHVQVGEAAK AAGIDRVLSV GKQSHAISTA
410 420 430 440 450
SGVGEHFADK TALITRLKLL IAEQQVITIL VKGSRSAAME EVVRALQENG

TC
Length:452
Mass (Da):47,447
Last modified:November 1, 1997 - v2
Checksum:iB46E2E57BDBBC572
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61G → A (PubMed:2668880).Curated1
Sequence conflicti61G → A (PubMed:1630901).Curated1
Sequence conflicti178A → R (PubMed:2668880).Curated1
Sequence conflicti178A → R (PubMed:1630901).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti288A → T in murF2; temperature-sensitive mutant with low activity. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15432 Genomic DNA. Translation: CAA33473.1.
X55034 Genomic DNA. Translation: CAA38863.1.
U67891 Genomic DNA. Translation: AAC44657.1.
U00096 Genomic DNA. Translation: AAC73197.1.
AP009048 Genomic DNA. Translation: BAB96654.2.
U67893 Genomic DNA. Translation: AAB60788.1.
PIRiF64730.
RefSeqiNP_414628.1. NC_000913.3.
WP_000626685.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73197; AAC73197; b0086.
BAB96654; BAB96654; BAB96654.
GeneIDi944813.
KEGGiecj:JW0084.
eco:b0086.
PATRICi32115277. VBIEscCol129921_0090.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15432 Genomic DNA. Translation: CAA33473.1.
X55034 Genomic DNA. Translation: CAA38863.1.
U67891 Genomic DNA. Translation: AAC44657.1.
U00096 Genomic DNA. Translation: AAC73197.1.
AP009048 Genomic DNA. Translation: BAB96654.2.
U67893 Genomic DNA. Translation: AAB60788.1.
PIRiF64730.
RefSeqiNP_414628.1. NC_000913.3.
WP_000626685.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GG4X-ray2.30A/B1-452[»]
ProteinModelPortaliP11880.
SMRiP11880.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261642. 416 interactors.
DIPiDIP-10281N.
IntActiP11880. 11 interactors.
MINTiMINT-1227223.
STRINGi511145.b0086.

Chemistry databases

BindingDBiP11880.
ChEMBLiCHEMBL2540.

Proteomic databases

EPDiP11880.
PaxDbiP11880.
PRIDEiP11880.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73197; AAC73197; b0086.
BAB96654; BAB96654; BAB96654.
GeneIDi944813.
KEGGiecj:JW0084.
eco:b0086.
PATRICi32115277. VBIEscCol129921_0090.

Organism-specific databases

EchoBASEiEB0617.
EcoGeneiEG10622. murF.

Phylogenomic databases

eggNOGiENOG4108EQQ. Bacteria.
COG0770. LUCA.
HOGENOMiHOG000268120.
InParanoidiP11880.
KOiK01929.
OMAiDYAVIEM.
PhylomeDBiP11880.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciEcoCyc:UDP-NACMURALGLDAPAALIG-MONOMER.
ECOL316407:JW0084-MONOMER.
MetaCyc:UDP-NACMURALGLDAPAALIG-MONOMER.
BRENDAi6.3.2.10. 2026.
6.3.2.8. 2026.
SABIO-RKP11880.

Miscellaneous databases

EvolutionaryTraceiP11880.
PROiP11880.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_02019. MurF. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005863. UDP-N-AcMur_synth.
[Graphical view]
PANTHERiPTHR23135:SF3. PTHR23135:SF3. 1 hit.
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01143. murF. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMURF_ECOLI
AccessioniPrimary (citable) accession number: P11880
Secondary accession number(s): O07100, P77636
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 165 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.