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Protein

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase

Gene

murF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.UniRule annotation1 Publication

Catalytic activityi

ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.UniRule annotation1 Publication

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi107 – 113ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan biosynthetic process Source: EcoCyc
  • regulation of cell shape Source: UniProtKB-KW

Keywordsi

Molecular functionLigase
Biological processCell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:UDP-NACMURALGLDAPAALIG-MONOMER
MetaCyc:UDP-NACMURALGLDAPAALIG-MONOMER
BRENDAi6.3.2.10 2026
6.3.2.8 2026
SABIO-RKiP11880
UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseUniRule annotationCurated (EC:6.3.2.10UniRule annotation1 Publication)
Alternative name(s):
D-alanyl-D-alanine-adding enzyme1 PublicationUniRule annotation
UDP-MurNAc-pentapeptide synthetase1 Publication
Gene namesi
Name:murF1 PublicationUniRule annotation
Synonyms:mra
Ordered Locus Names:b0086, JW0084
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10622 murF

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2540

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001016981 – 452UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseAdd BLAST452

Proteomic databases

EPDiP11880
PaxDbiP11880
PRIDEiP11880

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4261642, 419 interactors
DIPiDIP-10281N
IntActiP11880, 11 interactors
STRINGi316385.ECDH10B_0068

Chemistry databases

BindingDBiP11880

Structurei

Secondary structure

1452
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 12Combined sources7
Beta strandi16 – 19Combined sources4
Beta strandi23 – 25Combined sources3
Beta strandi27 – 29Combined sources3
Helixi31 – 33Combined sources3
Beta strandi39 – 41Combined sources3
Turni50 – 53Combined sources4
Helixi54 – 59Combined sources6
Beta strandi64 – 69Combined sources6
Beta strandi77 – 79Combined sources3
Helixi83 – 97Combined sources15
Beta strandi101 – 106Combined sources6
Helixi111 – 122Combined sources12
Turni123 – 125Combined sources3
Beta strandi128 – 130Combined sources3
Helixi140 – 145Combined sources6
Beta strandi153 – 158Combined sources6
Beta strandi162 – 165Combined sources4
Helixi166 – 174Combined sources9
Beta strandi177 – 181Combined sources5
Helixi195 – 203Combined sources9
Helixi204 – 208Combined sources5
Beta strandi214 – 218Combined sources5
Helixi224 – 231Combined sources8
Beta strandi234 – 239Combined sources6
Beta strandi247 – 255Combined sources9
Beta strandi257 – 266Combined sources10
Beta strandi269 – 275Combined sources7
Beta strandi277 – 279Combined sources3
Helixi281 – 295Combined sources15
Helixi300 – 307Combined sources8
Beta strandi316 – 323Combined sources8
Beta strandi326 – 330Combined sources5
Helixi337 – 349Combined sources13
Beta strandi350 – 358Combined sources9
Helixi367 – 382Combined sources16
Beta strandi385 – 393Combined sources9
Helixi395 – 400Combined sources6
Beta strandi405 – 409Combined sources5
Helixi410 – 423Combined sources14
Beta strandi425 – 432Combined sources8
Helixi435 – 437Combined sources3
Helixi440 – 446Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GG4X-ray2.30A/B1-452[»]
ProteinModelPortaliP11880
SMRiP11880
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11880

Family & Domainsi

Sequence similaritiesi

Belongs to the MurCDEF family. MurF subfamily.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4108EQQ Bacteria
COG0770 LUCA
HOGENOMiHOG000268120
InParanoidiP11880
KOiK01929
OMAiSYNNHWG
PhylomeDBiP11880

Family and domain databases

Gene3Di3.40.1190.10, 1 hit
3.90.190.20, 1 hit
HAMAPiMF_02019 MurF, 1 hit
InterProiView protein in InterPro
IPR036565 Mur-like_cat_sf
IPR004101 Mur_ligase_C
IPR036615 Mur_ligase_C_dom_sf
IPR013221 Mur_ligase_cen
IPR000713 Mur_ligase_N
IPR035911 MurE/MurF_N
IPR005863 UDP-N-AcMur_synth
PfamiView protein in Pfam
PF01225 Mur_ligase, 1 hit
PF02875 Mur_ligase_C, 1 hit
PF08245 Mur_ligase_M, 1 hit
SUPFAMiSSF53244 SSF53244, 1 hit
SSF53623 SSF53623, 1 hit
SSF63418 SSF63418, 1 hit
TIGRFAMsiTIGR01143 murF, 1 hit

Sequencei

Sequence statusi: Complete.

P11880-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISVTLSQLT DILNGELQGA DITLDAVTTD TRKLTPGCLF VALKGERFDA
60 70 80 90 100
HDFADQAKAG GAGALLVSRP LDIDLPQLIV KDTRLAFGEL AAWVRQQVPA
110 120 130 140 150
RVVALTGSSG KTSVKEMTAA ILSQCGNTLY TAGNLNNDIG VPMTLLRLTP
160 170 180 190 200
EYDYAVIELG ANHQGEIAWT VSLTRPEAAL VNNLAAAHLE GFGSLAGVAK
210 220 230 240 250
AKGEIFSGLP ENGIAIMNAD NNDWLNWQSV IGSRKVWRFS PNAANSDFTA
260 270 280 290 300
TNIHVTSHGT EFTLQTPTGS VDVLLPLPGR HNIANALAAA ALSMSVGATL
310 320 330 340 350
DAIKAGLANL KAVPGRLFPI QLAENQLLLD DSYNANVGSM TAAVQVLAEM
360 370 380 390 400
PGYRVLVVGD MAELGAESEA CHVQVGEAAK AAGIDRVLSV GKQSHAISTA
410 420 430 440 450
SGVGEHFADK TALITRLKLL IAEQQVITIL VKGSRSAAME EVVRALQENG

TC
Length:452
Mass (Da):47,447
Last modified:November 1, 1997 - v2
Checksum:iB46E2E57BDBBC572
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61G → A (PubMed:2668880).Curated1
Sequence conflicti61G → A (PubMed:1630901).Curated1
Sequence conflicti178A → R (PubMed:2668880).Curated1
Sequence conflicti178A → R (PubMed:1630901).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti288A → T in murF2; temperature-sensitive mutant with low activity. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15432 Genomic DNA Translation: CAA33473.1
X55034 Genomic DNA Translation: CAA38863.1
U67891 Genomic DNA Translation: AAC44657.1
U00096 Genomic DNA Translation: AAC73197.1
AP009048 Genomic DNA Translation: BAB96654.2
U67893 Genomic DNA Translation: AAB60788.1
PIRiF64730
RefSeqiNP_414628.1, NC_000913.3
WP_000626685.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73197; AAC73197; b0086
BAB96654; BAB96654; BAB96654
GeneIDi944813
KEGGiecj:JW0084
eco:b0086
PATRICifig|1411691.4.peg.2194

Similar proteinsi

Entry informationi

Entry nameiMURF_ECOLI
AccessioniPrimary (citable) accession number: P11880
Secondary accession number(s): O07100, P77636
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health