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P11875

- SYR_ECOLI

UniProt

P11875 - SYR_ECOLI

Protein

Arginine--tRNA ligase

Gene

argS

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 1 (01 Oct 1989)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).

    GO - Molecular functioni

    1. arginine-tRNA ligase activity Source: EcoCyc
    2. ATP binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. arginyl-tRNA aminoacylation Source: EcoCyc

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:ARGS-MONOMER.
    ECOL316407:JW1865-MONOMER.
    MetaCyc:ARGS-MONOMER.
    SABIO-RKP11875.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arginine--tRNA ligase (EC:6.1.1.19)
    Alternative name(s):
    Arginyl-tRNA synthetase
    Short name:
    ArgRS
    Gene namesi
    Name:argS
    Ordered Locus Names:b1876, JW1865
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10071. argS.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 577577Arginine--tRNA ligasePRO_0000151557Add
    BLAST

    Proteomic databases

    PaxDbiP11875.
    PRIDEiP11875.

    2D gel databases

    SWISS-2DPAGEP11875.

    Expressioni

    Gene expression databases

    GenevestigatoriP11875.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    DIPiDIP-9147N.
    IntActiP11875. 10 interactions.
    MINTiMINT-1241236.
    STRINGi511145.b1876.

    Structurei

    Secondary structure

    1
    577
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 1715
    Beta strandi28 – 303
    Helixi34 – 363
    Beta strandi38 – 414
    Helixi44 – 507
    Helixi55 – 6511
    Turni69 – 713
    Beta strandi72 – 787
    Turni79 – 813
    Beta strandi82 – 876
    Helixi89 – 10113
    Turni103 – 1064
    Beta strandi114 – 1185
    Helixi130 – 14920
    Beta strandi152 – 1554
    Helixi165 – 17612
    Helixi189 – 20113
    Helixi206 – 21813
    Helixi222 – 24524
    Helixi252 – 2543
    Helixi258 – 2647
    Helixi265 – 2739
    Beta strandi281 – 2899
    Helixi290 – 2923
    Beta strandi300 – 3056
    Helixi313 – 32614
    Beta strandi331 – 3355
    Helixi339 – 3413
    Helixi342 – 35413
    Beta strandi360 – 3623
    Helixi390 – 40516
    Turni413 – 4164
    Helixi417 – 43418
    Helixi446 – 4494
    Beta strandi452 – 4554
    Helixi457 – 47216
    Helixi478 – 4814
    Helixi491 – 50111
    Helixi503 – 51311
    Helixi517 – 53519
    Helixi546 – 56924

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4OBYX-ray2.57A1-577[»]
    ProteinModelPortaliP11875.
    SMRiP11875. Positions 1-576.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi122 – 13211"HIGH" regionAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0018.
    HOGENOMiHOG000247212.
    KOiK01887.
    OMAiMEHMGFG.
    OrthoDBiEOG6JB13C.
    PhylomeDBiP11875.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.30.1360.70. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPiMF_00123. Arg_tRNA_synth.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR001278. Arg-tRNA-ligase.
    IPR005148. Arg-tRNA-synth_N.
    IPR008909. DALR_anticod-bd.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view]
    PANTHERiPTHR11956. PTHR11956. 1 hit.
    PfamiPF03485. Arg_tRNA_synt_N. 1 hit.
    PF05746. DALR_1. 1 hit.
    PF00750. tRNA-synt_1d. 1 hit.
    [Graphical view]
    PRINTSiPR01038. TRNASYNTHARG.
    SMARTiSM01016. Arg_tRNA_synt_N. 1 hit.
    SM00836. DALR_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF55190. SSF55190. 1 hit.
    TIGRFAMsiTIGR00456. argS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P11875-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNIQALLSEK VRQAMIAAGA PADCEPQVRQ SAKVQFGDYQ ANGMMAVAKK    50
    LGMAPRQLAE QVLTHLDLNG IASKVEIAGP GFINIFLDPA FLAEHVQQAL 100
    ASDRLGVATP EKQTIVVDYS APNVAKEMHV GHLRSTIIGD AAVRTLEFLG 150
    HKVIRANHVG DWGTQFGMLI AWLEKQQQEN AGEMELADLE GFYRDAKKHY 200
    DEDEEFAERA RNYVVKLQSG DEYFREMWRK LVDITMTQNQ ITYDRLNVTL 250
    TRDDVMGESL YNPMLPGIVA DLKAKGLAVE SEGATVVFLD EFKNKEGEPM 300
    GVIIQKKDGG YLYTTTDIAC AKYRYETLHA DRVLYYIDSR QHQHLMQAWA 350
    IVRKAGYVPE SVPLEHHMFG MMLGKDGKPF KTRAGGTVKL ADLLDEALER 400
    ARRLVAEKNP DMPADELEKL ANAVGIGAVK YADLSKNRTT DYIFDWDNML 450
    AFEGNTAPYM QYAYTRVLSV FRKAEIDEEQ LAAAPVIIRE DREAQLAARL 500
    LQFEETLTVV AREGTPHVMC AYLYDLAGLF SGFYEHCPIL SAENEEVRNS 550
    RLKLAQLTAK TLKLGLDTLG IETVERM 577
    Length:577
    Mass (Da):64,683
    Last modified:October 1, 1989 - v1
    Checksum:iC107D346660CF9B8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15320 Genomic DNA. Translation: CAA33384.1.
    U00096 Genomic DNA. Translation: AAC74946.1.
    AP009048 Genomic DNA. Translation: BAA15686.1.
    PIRiJS0267. SYECRT.
    RefSeqiNP_416390.1. NC_000913.3.
    YP_490138.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74946; AAC74946; b1876.
    BAA15686; BAA15686; BAA15686.
    GeneIDi12931374.
    946452.
    KEGGiecj:Y75_p1852.
    eco:b1876.
    PATRICi32119077. VBIEscCol129921_1956.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15320 Genomic DNA. Translation: CAA33384.1 .
    U00096 Genomic DNA. Translation: AAC74946.1 .
    AP009048 Genomic DNA. Translation: BAA15686.1 .
    PIRi JS0267. SYECRT.
    RefSeqi NP_416390.1. NC_000913.3.
    YP_490138.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4OBY X-ray 2.57 A 1-577 [» ]
    ProteinModelPortali P11875.
    SMRi P11875. Positions 1-576.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9147N.
    IntActi P11875. 10 interactions.
    MINTi MINT-1241236.
    STRINGi 511145.b1876.

    2D gel databases

    SWISS-2DPAGE P11875.

    Proteomic databases

    PaxDbi P11875.
    PRIDEi P11875.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74946 ; AAC74946 ; b1876 .
    BAA15686 ; BAA15686 ; BAA15686 .
    GeneIDi 12931374.
    946452.
    KEGGi ecj:Y75_p1852.
    eco:b1876.
    PATRICi 32119077. VBIEscCol129921_1956.

    Organism-specific databases

    EchoBASEi EB0069.
    EcoGenei EG10071. argS.

    Phylogenomic databases

    eggNOGi COG0018.
    HOGENOMi HOG000247212.
    KOi K01887.
    OMAi MEHMGFG.
    OrthoDBi EOG6JB13C.
    PhylomeDBi P11875.

    Enzyme and pathway databases

    BioCyci EcoCyc:ARGS-MONOMER.
    ECOL316407:JW1865-MONOMER.
    MetaCyc:ARGS-MONOMER.
    SABIO-RK P11875.

    Miscellaneous databases

    PROi P11875.

    Gene expression databases

    Genevestigatori P11875.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.30.1360.70. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPi MF_00123. Arg_tRNA_synth.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR001278. Arg-tRNA-ligase.
    IPR005148. Arg-tRNA-synth_N.
    IPR008909. DALR_anticod-bd.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view ]
    PANTHERi PTHR11956. PTHR11956. 1 hit.
    Pfami PF03485. Arg_tRNA_synt_N. 1 hit.
    PF05746. DALR_1. 1 hit.
    PF00750. tRNA-synt_1d. 1 hit.
    [Graphical view ]
    PRINTSi PR01038. TRNASYNTHARG.
    SMARTi SM01016. Arg_tRNA_synt_N. 1 hit.
    SM00836. DALR_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF55190. SSF55190. 1 hit.
    TIGRFAMsi TIGR00456. argS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of the gene coding for Escherichia coli arginyl-tRNA synthetase."
      Eriani G., Dirheimer G., Gangloff J.
      Nucleic Acids Res. 17:5725-5736(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-19.
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Escherichia coli proteome analysis using the gene-protein database."
      VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
      Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY 2D-GEL.

    Entry informationi

    Entry nameiSYR_ECOLI
    AccessioniPrimary (citable) accession number: P11875
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3