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Protein

Arginine--tRNA ligase

Gene

argS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).

GO - Molecular functioni

  • arginine-tRNA ligase activity Source: EcoCyc
  • ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  • arginyl-tRNA aminoacylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ARGS-MONOMER.
ECOL316407:JW1865-MONOMER.
MetaCyc:ARGS-MONOMER.
BRENDAi6.1.1.19. 2026.
SABIO-RKP11875.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine--tRNA ligase (EC:6.1.1.19)
Alternative name(s):
Arginyl-tRNA synthetase
Short name:
ArgRS
Gene namesi
Name:argS
Ordered Locus Names:b1876, JW1865
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10071. argS.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001515571 – 577Arginine--tRNA ligaseAdd BLAST577

Proteomic databases

EPDiP11875.
PaxDbiP11875.
PRIDEiP11875.

2D gel databases

SWISS-2DPAGEP11875.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

DIPiDIP-9147N.
IntActiP11875. 10 interactors.
MINTiMINT-1241236.
STRINGi511145.b1876.

Structurei

Secondary structure

1577
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 17Combined sources15
Beta strandi28 – 30Combined sources3
Helixi34 – 36Combined sources3
Beta strandi38 – 41Combined sources4
Helixi44 – 50Combined sources7
Helixi55 – 65Combined sources11
Turni69 – 71Combined sources3
Beta strandi72 – 78Combined sources7
Turni79 – 81Combined sources3
Beta strandi82 – 87Combined sources6
Helixi89 – 101Combined sources13
Turni103 – 106Combined sources4
Beta strandi114 – 118Combined sources5
Helixi130 – 149Combined sources20
Beta strandi152 – 155Combined sources4
Helixi165 – 176Combined sources12
Helixi189 – 201Combined sources13
Helixi206 – 218Combined sources13
Helixi222 – 245Combined sources24
Helixi252 – 254Combined sources3
Helixi258 – 264Combined sources7
Helixi265 – 273Combined sources9
Beta strandi281 – 289Combined sources9
Helixi290 – 292Combined sources3
Beta strandi300 – 305Combined sources6
Helixi313 – 326Combined sources14
Beta strandi331 – 335Combined sources5
Helixi339 – 341Combined sources3
Helixi342 – 354Combined sources13
Beta strandi360 – 362Combined sources3
Helixi390 – 405Combined sources16
Turni413 – 416Combined sources4
Helixi417 – 434Combined sources18
Helixi446 – 449Combined sources4
Beta strandi452 – 455Combined sources4
Helixi457 – 472Combined sources16
Helixi478 – 481Combined sources4
Helixi491 – 501Combined sources11
Helixi503 – 513Combined sources11
Helixi517 – 535Combined sources19
Helixi546 – 569Combined sources24

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OBYX-ray2.57A1-577[»]
ProteinModelPortaliP11875.
SMRiP11875.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi122 – 132"HIGH" regionAdd BLAST11

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C75. Bacteria.
COG0018. LUCA.
HOGENOMiHOG000247212.
InParanoidiP11875.
KOiK01887.
OMAiHVGHIRN.
PhylomeDBiP11875.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00123. Arg_tRNA_synth. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR11956. PTHR11956. 1 hit.
PfamiPF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSiPR01038. TRNASYNTHARG.
SMARTiSM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsiTIGR00456. argS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11875-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIQALLSEK VRQAMIAAGA PADCEPQVRQ SAKVQFGDYQ ANGMMAVAKK
60 70 80 90 100
LGMAPRQLAE QVLTHLDLNG IASKVEIAGP GFINIFLDPA FLAEHVQQAL
110 120 130 140 150
ASDRLGVATP EKQTIVVDYS APNVAKEMHV GHLRSTIIGD AAVRTLEFLG
160 170 180 190 200
HKVIRANHVG DWGTQFGMLI AWLEKQQQEN AGEMELADLE GFYRDAKKHY
210 220 230 240 250
DEDEEFAERA RNYVVKLQSG DEYFREMWRK LVDITMTQNQ ITYDRLNVTL
260 270 280 290 300
TRDDVMGESL YNPMLPGIVA DLKAKGLAVE SEGATVVFLD EFKNKEGEPM
310 320 330 340 350
GVIIQKKDGG YLYTTTDIAC AKYRYETLHA DRVLYYIDSR QHQHLMQAWA
360 370 380 390 400
IVRKAGYVPE SVPLEHHMFG MMLGKDGKPF KTRAGGTVKL ADLLDEALER
410 420 430 440 450
ARRLVAEKNP DMPADELEKL ANAVGIGAVK YADLSKNRTT DYIFDWDNML
460 470 480 490 500
AFEGNTAPYM QYAYTRVLSV FRKAEIDEEQ LAAAPVIIRE DREAQLAARL
510 520 530 540 550
LQFEETLTVV AREGTPHVMC AYLYDLAGLF SGFYEHCPIL SAENEEVRNS
560 570
RLKLAQLTAK TLKLGLDTLG IETVERM
Length:577
Mass (Da):64,683
Last modified:October 1, 1989 - v1
Checksum:iC107D346660CF9B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15320 Genomic DNA. Translation: CAA33384.1.
U00096 Genomic DNA. Translation: AAC74946.1.
AP009048 Genomic DNA. Translation: BAA15686.1.
PIRiJS0267. SYECRT.
RefSeqiNP_416390.1. NC_000913.3.
WP_001025322.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74946; AAC74946; b1876.
BAA15686; BAA15686; BAA15686.
GeneIDi946452.
KEGGiecj:JW1865.
eco:b1876.
PATRICi32119077. VBIEscCol129921_1956.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15320 Genomic DNA. Translation: CAA33384.1.
U00096 Genomic DNA. Translation: AAC74946.1.
AP009048 Genomic DNA. Translation: BAA15686.1.
PIRiJS0267. SYECRT.
RefSeqiNP_416390.1. NC_000913.3.
WP_001025322.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OBYX-ray2.57A1-577[»]
ProteinModelPortaliP11875.
SMRiP11875.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-9147N.
IntActiP11875. 10 interactors.
MINTiMINT-1241236.
STRINGi511145.b1876.

2D gel databases

SWISS-2DPAGEP11875.

Proteomic databases

EPDiP11875.
PaxDbiP11875.
PRIDEiP11875.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74946; AAC74946; b1876.
BAA15686; BAA15686; BAA15686.
GeneIDi946452.
KEGGiecj:JW1865.
eco:b1876.
PATRICi32119077. VBIEscCol129921_1956.

Organism-specific databases

EchoBASEiEB0069.
EcoGeneiEG10071. argS.

Phylogenomic databases

eggNOGiENOG4105C75. Bacteria.
COG0018. LUCA.
HOGENOMiHOG000247212.
InParanoidiP11875.
KOiK01887.
OMAiHVGHIRN.
PhylomeDBiP11875.

Enzyme and pathway databases

BioCyciEcoCyc:ARGS-MONOMER.
ECOL316407:JW1865-MONOMER.
MetaCyc:ARGS-MONOMER.
BRENDAi6.1.1.19. 2026.
SABIO-RKP11875.

Miscellaneous databases

PROiP11875.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00123. Arg_tRNA_synth. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR11956. PTHR11956. 1 hit.
PfamiPF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSiPR01038. TRNASYNTHARG.
SMARTiSM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsiTIGR00456. argS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYR_ECOLI
AccessioniPrimary (citable) accession number: P11875
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 2, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.