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P11875

- SYR_ECOLI

UniProt

P11875 - SYR_ECOLI

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Protein
Arginine--tRNA ligase
Gene
argS, b1876, JW1865
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).UniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. arginine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginyl-tRNA aminoacylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ARGS-MONOMER.
ECOL316407:JW1865-MONOMER.
MetaCyc:ARGS-MONOMER.
SABIO-RKP11875.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine--tRNA ligase (EC:6.1.1.19)
Alternative name(s):
Arginyl-tRNA synthetase
Short name:
ArgRS
Gene namesi
Name:argS
Ordered Locus Names:b1876, JW1865
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10071. argS.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 577577Arginine--tRNA ligaseUniRule annotation
PRO_0000151557Add
BLAST

Proteomic databases

PaxDbiP11875.
PRIDEiP11875.

2D gel databases

SWISS-2DPAGEP11875.

Expressioni

Gene expression databases

GenevestigatoriP11875.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

DIPiDIP-9147N.
IntActiP11875. 10 interactions.
MINTiMINT-1241236.
STRINGi511145.b1876.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1715
Beta strandi28 – 303
Helixi34 – 363
Beta strandi38 – 414
Helixi44 – 507
Helixi55 – 6511
Turni69 – 713
Beta strandi72 – 787
Turni79 – 813
Beta strandi82 – 876
Helixi89 – 10113
Turni103 – 1064
Beta strandi114 – 1185
Helixi130 – 14920
Beta strandi152 – 1554
Helixi165 – 17612
Helixi189 – 20113
Helixi206 – 21813
Helixi222 – 24524
Helixi252 – 2543
Helixi258 – 2647
Helixi265 – 2739
Beta strandi281 – 2899
Helixi290 – 2923
Beta strandi300 – 3056
Helixi313 – 32614
Beta strandi331 – 3355
Helixi339 – 3413
Helixi342 – 35413
Beta strandi360 – 3623
Helixi390 – 40516
Turni413 – 4164
Helixi417 – 43418
Helixi446 – 4494
Beta strandi452 – 4554
Helixi457 – 47216
Helixi478 – 4814
Helixi491 – 50111
Helixi503 – 51311
Helixi517 – 53519
Helixi546 – 56924

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4OBYX-ray2.57A1-577[»]
ProteinModelPortaliP11875.
SMRiP11875. Positions 1-576.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi122 – 13211"HIGH" regionUniRule annotation
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0018.
HOGENOMiHOG000247212.
KOiK01887.
OMAiMEHMGFG.
OrthoDBiEOG6JB13C.
PhylomeDBiP11875.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00123. Arg_tRNA_synth.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR11956. PTHR11956. 1 hit.
PfamiPF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSiPR01038. TRNASYNTHARG.
SMARTiSM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsiTIGR00456. argS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11875-1 [UniParc]FASTAAdd to Basket

« Hide

MNIQALLSEK VRQAMIAAGA PADCEPQVRQ SAKVQFGDYQ ANGMMAVAKK    50
LGMAPRQLAE QVLTHLDLNG IASKVEIAGP GFINIFLDPA FLAEHVQQAL 100
ASDRLGVATP EKQTIVVDYS APNVAKEMHV GHLRSTIIGD AAVRTLEFLG 150
HKVIRANHVG DWGTQFGMLI AWLEKQQQEN AGEMELADLE GFYRDAKKHY 200
DEDEEFAERA RNYVVKLQSG DEYFREMWRK LVDITMTQNQ ITYDRLNVTL 250
TRDDVMGESL YNPMLPGIVA DLKAKGLAVE SEGATVVFLD EFKNKEGEPM 300
GVIIQKKDGG YLYTTTDIAC AKYRYETLHA DRVLYYIDSR QHQHLMQAWA 350
IVRKAGYVPE SVPLEHHMFG MMLGKDGKPF KTRAGGTVKL ADLLDEALER 400
ARRLVAEKNP DMPADELEKL ANAVGIGAVK YADLSKNRTT DYIFDWDNML 450
AFEGNTAPYM QYAYTRVLSV FRKAEIDEEQ LAAAPVIIRE DREAQLAARL 500
LQFEETLTVV AREGTPHVMC AYLYDLAGLF SGFYEHCPIL SAENEEVRNS 550
RLKLAQLTAK TLKLGLDTLG IETVERM 577
Length:577
Mass (Da):64,683
Last modified:October 1, 1989 - v1
Checksum:iC107D346660CF9B8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15320 Genomic DNA. Translation: CAA33384.1.
U00096 Genomic DNA. Translation: AAC74946.1.
AP009048 Genomic DNA. Translation: BAA15686.1.
PIRiJS0267. SYECRT.
RefSeqiNP_416390.1. NC_000913.3.
YP_490138.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74946; AAC74946; b1876.
BAA15686; BAA15686; BAA15686.
GeneIDi12931374.
946452.
KEGGiecj:Y75_p1852.
eco:b1876.
PATRICi32119077. VBIEscCol129921_1956.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15320 Genomic DNA. Translation: CAA33384.1 .
U00096 Genomic DNA. Translation: AAC74946.1 .
AP009048 Genomic DNA. Translation: BAA15686.1 .
PIRi JS0267. SYECRT.
RefSeqi NP_416390.1. NC_000913.3.
YP_490138.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4OBY X-ray 2.57 A 1-577 [» ]
ProteinModelPortali P11875.
SMRi P11875. Positions 1-576.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-9147N.
IntActi P11875. 10 interactions.
MINTi MINT-1241236.
STRINGi 511145.b1876.

2D gel databases

SWISS-2DPAGE P11875.

Proteomic databases

PaxDbi P11875.
PRIDEi P11875.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74946 ; AAC74946 ; b1876 .
BAA15686 ; BAA15686 ; BAA15686 .
GeneIDi 12931374.
946452.
KEGGi ecj:Y75_p1852.
eco:b1876.
PATRICi 32119077. VBIEscCol129921_1956.

Organism-specific databases

EchoBASEi EB0069.
EcoGenei EG10071. argS.

Phylogenomic databases

eggNOGi COG0018.
HOGENOMi HOG000247212.
KOi K01887.
OMAi MEHMGFG.
OrthoDBi EOG6JB13C.
PhylomeDBi P11875.

Enzyme and pathway databases

BioCyci EcoCyc:ARGS-MONOMER.
ECOL316407:JW1865-MONOMER.
MetaCyc:ARGS-MONOMER.
SABIO-RK P11875.

Miscellaneous databases

PROi P11875.

Gene expression databases

Genevestigatori P11875.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPi MF_00123. Arg_tRNA_synth.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view ]
PANTHERi PTHR11956. PTHR11956. 1 hit.
Pfami PF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view ]
PRINTSi PR01038. TRNASYNTHARG.
SMARTi SM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view ]
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsi TIGR00456. argS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the gene coding for Escherichia coli arginyl-tRNA synthetase."
    Eriani G., Dirheimer G., Gangloff J.
    Nucleic Acids Res. 17:5725-5736(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-19.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.

Entry informationi

Entry nameiSYR_ECOLI
AccessioniPrimary (citable) accession number: P11875
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: July 9, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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