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Protein

Arginine--tRNA ligase

Gene

argS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).

GO - Molecular functioni

  • arginine-tRNA ligase activity Source: EcoCyc
  • ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  • arginyl-tRNA aminoacylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ARGS-MONOMER.
ECOL316407:JW1865-MONOMER.
MetaCyc:ARGS-MONOMER.
BRENDAi6.1.1.19. 2026.
SABIO-RKP11875.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine--tRNA ligase (EC:6.1.1.19)
Alternative name(s):
Arginyl-tRNA synthetase
Short name:
ArgRS
Gene namesi
Name:argS
Ordered Locus Names:b1876, JW1865
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10071. argS.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 577577Arginine--tRNA ligasePRO_0000151557Add
BLAST

Proteomic databases

PaxDbiP11875.
PRIDEiP11875.

2D gel databases

SWISS-2DPAGEP11875.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

DIPiDIP-9147N.
IntActiP11875. 10 interactions.
MINTiMINT-1241236.
STRINGi511145.b1876.

Structurei

Secondary structure

1
577
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1715Combined sources
Beta strandi28 – 303Combined sources
Helixi34 – 363Combined sources
Beta strandi38 – 414Combined sources
Helixi44 – 507Combined sources
Helixi55 – 6511Combined sources
Turni69 – 713Combined sources
Beta strandi72 – 787Combined sources
Turni79 – 813Combined sources
Beta strandi82 – 876Combined sources
Helixi89 – 10113Combined sources
Turni103 – 1064Combined sources
Beta strandi114 – 1185Combined sources
Helixi130 – 14920Combined sources
Beta strandi152 – 1554Combined sources
Helixi165 – 17612Combined sources
Helixi189 – 20113Combined sources
Helixi206 – 21813Combined sources
Helixi222 – 24524Combined sources
Helixi252 – 2543Combined sources
Helixi258 – 2647Combined sources
Helixi265 – 2739Combined sources
Beta strandi281 – 2899Combined sources
Helixi290 – 2923Combined sources
Beta strandi300 – 3056Combined sources
Helixi313 – 32614Combined sources
Beta strandi331 – 3355Combined sources
Helixi339 – 3413Combined sources
Helixi342 – 35413Combined sources
Beta strandi360 – 3623Combined sources
Helixi390 – 40516Combined sources
Turni413 – 4164Combined sources
Helixi417 – 43418Combined sources
Helixi446 – 4494Combined sources
Beta strandi452 – 4554Combined sources
Helixi457 – 47216Combined sources
Helixi478 – 4814Combined sources
Helixi491 – 50111Combined sources
Helixi503 – 51311Combined sources
Helixi517 – 53519Combined sources
Helixi546 – 56924Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4OBYX-ray2.57A1-577[»]
ProteinModelPortaliP11875.
SMRiP11875. Positions 1-576.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi122 – 13211"HIGH" regionAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0018.
HOGENOMiHOG000247212.
InParanoidiP11875.
KOiK01887.
OMAiNPNGPLH.
OrthoDBiEOG6JB13C.
PhylomeDBiP11875.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00123. Arg_tRNA_synth.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR11956. PTHR11956. 1 hit.
PfamiPF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSiPR01038. TRNASYNTHARG.
SMARTiSM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsiTIGR00456. argS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11875-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIQALLSEK VRQAMIAAGA PADCEPQVRQ SAKVQFGDYQ ANGMMAVAKK
60 70 80 90 100
LGMAPRQLAE QVLTHLDLNG IASKVEIAGP GFINIFLDPA FLAEHVQQAL
110 120 130 140 150
ASDRLGVATP EKQTIVVDYS APNVAKEMHV GHLRSTIIGD AAVRTLEFLG
160 170 180 190 200
HKVIRANHVG DWGTQFGMLI AWLEKQQQEN AGEMELADLE GFYRDAKKHY
210 220 230 240 250
DEDEEFAERA RNYVVKLQSG DEYFREMWRK LVDITMTQNQ ITYDRLNVTL
260 270 280 290 300
TRDDVMGESL YNPMLPGIVA DLKAKGLAVE SEGATVVFLD EFKNKEGEPM
310 320 330 340 350
GVIIQKKDGG YLYTTTDIAC AKYRYETLHA DRVLYYIDSR QHQHLMQAWA
360 370 380 390 400
IVRKAGYVPE SVPLEHHMFG MMLGKDGKPF KTRAGGTVKL ADLLDEALER
410 420 430 440 450
ARRLVAEKNP DMPADELEKL ANAVGIGAVK YADLSKNRTT DYIFDWDNML
460 470 480 490 500
AFEGNTAPYM QYAYTRVLSV FRKAEIDEEQ LAAAPVIIRE DREAQLAARL
510 520 530 540 550
LQFEETLTVV AREGTPHVMC AYLYDLAGLF SGFYEHCPIL SAENEEVRNS
560 570
RLKLAQLTAK TLKLGLDTLG IETVERM
Length:577
Mass (Da):64,683
Last modified:October 1, 1989 - v1
Checksum:iC107D346660CF9B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15320 Genomic DNA. Translation: CAA33384.1.
U00096 Genomic DNA. Translation: AAC74946.1.
AP009048 Genomic DNA. Translation: BAA15686.1.
PIRiJS0267. SYECRT.
RefSeqiNP_416390.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74946; AAC74946; b1876.
BAA15686; BAA15686; BAA15686.
GeneIDi946452.
KEGGiecj:Y75_p1852.
eco:b1876.
PATRICi32119077. VBIEscCol129921_1956.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15320 Genomic DNA. Translation: CAA33384.1.
U00096 Genomic DNA. Translation: AAC74946.1.
AP009048 Genomic DNA. Translation: BAA15686.1.
PIRiJS0267. SYECRT.
RefSeqiNP_416390.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4OBYX-ray2.57A1-577[»]
ProteinModelPortaliP11875.
SMRiP11875. Positions 1-576.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-9147N.
IntActiP11875. 10 interactions.
MINTiMINT-1241236.
STRINGi511145.b1876.

2D gel databases

SWISS-2DPAGEP11875.

Proteomic databases

PaxDbiP11875.
PRIDEiP11875.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74946; AAC74946; b1876.
BAA15686; BAA15686; BAA15686.
GeneIDi946452.
KEGGiecj:Y75_p1852.
eco:b1876.
PATRICi32119077. VBIEscCol129921_1956.

Organism-specific databases

EchoBASEiEB0069.
EcoGeneiEG10071. argS.

Phylogenomic databases

eggNOGiCOG0018.
HOGENOMiHOG000247212.
InParanoidiP11875.
KOiK01887.
OMAiNPNGPLH.
OrthoDBiEOG6JB13C.
PhylomeDBiP11875.

Enzyme and pathway databases

BioCyciEcoCyc:ARGS-MONOMER.
ECOL316407:JW1865-MONOMER.
MetaCyc:ARGS-MONOMER.
BRENDAi6.1.1.19. 2026.
SABIO-RKP11875.

Miscellaneous databases

PROiP11875.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00123. Arg_tRNA_synth.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR11956. PTHR11956. 1 hit.
PfamiPF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSiPR01038. TRNASYNTHARG.
SMARTiSM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsiTIGR00456. argS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the gene coding for Escherichia coli arginyl-tRNA synthetase."
    Eriani G., Dirheimer G., Gangloff J.
    Nucleic Acids Res. 17:5725-5736(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-19.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.

Entry informationi

Entry nameiSYR_ECOLI
AccessioniPrimary (citable) accession number: P11875
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 24, 2015
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.