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Protein

Growth arrest-specific protein 2

Gene

Gas2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May play a role in chondrocyte proliferation and differentiation, and in limb myogenesis. May be involved in the regulation of the apoptosis in the interdigital tissues of the developing hindlimb. May be involved in the membrane ruffling process.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis, Cell cycle, Cell shape, Growth arrest

Enzyme and pathway databases

ReactomeiR-MMU-264870. Caspase-mediated cleavage of cytoskeletal proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Growth arrest-specific protein 2
Short name:
GAS-2
Gene namesi
Name:Gas2
Synonyms:Gas-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:95657. Gas2.

Subcellular locationi

  • Cytoplasmcytoskeleton 1 Publication
  • Membrane 1 Publication; Peripheral membrane protein 1 Publication

  • Note: Component of the microfilament system. Colocalizes with actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Mainly membrane-associated. When hyperphosphorylated, accumulates at membrane ruffles.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi279 – 2791D → A: Abolishes proteolytic processing.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314Growth arrest-specific protein 2PRO_0000190441Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei184 – 1841PhosphoserineCombined sources
Modified residuei188 – 1881PhosphoserineCombined sources

Post-translational modificationi

Cleaved, during apoptosis, on a specific aspartic residue by caspases.By similarity
Phosphorylated on serine residues during the G0-G1 transition phase.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei279 – 2802Cleavage; by a caspase during apoptosis

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP11862.
MaxQBiP11862.
PaxDbiP11862.
PRIDEiP11862.

PTM databases

iPTMnetiP11862.
PhosphoSiteiP11862.

Expressioni

Tissue specificityi

Expressed in most tissues. Highest levels in liver, lung and kidney. In the embryo strongly expressed in regions that undergo extensive apoptosis, such as the intervertebral tissues, the cranofacial mesenchyme and the cartilage of the limbs.

Developmental stagei

At embryonic days E11.5 and E13.5 strongly expressed in the soft connective tissue of the face and trunk, and in the invertebral tissues. Low levels are found in brain and neural tube. Low levels are found in day E13.5 lung, kidney, eye lens and in vertebral cartilage located cranially. In day E11.5 hindlimbs weakly expressed by the mesenchymal cells surrounding the perspective cartilage-forming regions. In day E12.5 hindlimbs strongly expressed by cells enveloping the chondrogenic primordia of the digits, metatarsals, tibia, and femur, and the soft connective tissue in the interdigital tissues. In day E13.5 hindlimbs expression is maintained in the intergigital tissues located proximally and is found in some chondrocytes in te stylopod and in mesenchymal cells surrounding the cartilage in the autopod and zygopod. In day E13.5 forelimb strongly expressed in the pre-hypertrophic and hypertrophic regions of the humerus, radius, and ulna. Expression in hypertrophic chondrocytes is maintained at day E14.5 and is not detectable at day E15.5. At day E14.5 also expressed by chondrocytes in the cartilage forming the carpals and tarsals and by mesenchymal cells in the process of condensing to form tendons. In day E13.5 hindlimbs expressed in some myoblasts in the proximal myogenic region. In older limbs expression is maintained in the myotubules.1 Publication

Inductioni

Down-regulated by mitogens.

Gene expression databases

BgeeiP11862.
CleanExiMM_GAS2.
ExpressionAtlasiP11862. baseline and differential.
GenevisibleiP11862. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000053514.

Structurei

Secondary structure

1
314
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi202 – 21110Combined sources
Beta strandi218 – 2203Combined sources
Beta strandi224 – 2285Combined sources
Beta strandi231 – 2344Combined sources
Beta strandi237 – 2448Combined sources
Turni245 – 2473Combined sources
Beta strandi248 – 2536Combined sources
Beta strandi256 – 2594Combined sources
Helixi260 – 2678Combined sources
Helixi269 – 2724Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V5RNMR-A201-284[»]
ProteinModelPortaliP11862.
SMRiP11862. Positions 193-284.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11862.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 157123CHPROSITE-ProRule annotationAdd
BLAST
Domaini198 – 27174GARPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the GAS2 family.Curated
Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation
Contains 1 GAR domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410KD4B. Eukaryota.
ENOG4111BF1. LUCA.
GeneTreeiENSGT00390000002205.
HOGENOMiHOG000007404.
HOVERGENiHBG005805.
InParanoidiP11862.
OMAiKFKESME.
PhylomeDBiP11862.
TreeFamiTF323754.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
3.30.920.20. 1 hit.
InterProiIPR001715. CH-domain.
IPR029929. GAS2.
IPR003108. GAS2_dom.
[Graphical view]
PANTHERiPTHR11915:SF298. PTHR11915:SF298. 1 hit.
PfamiPF00307. CH. 1 hit.
PF02187. GAS2. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 1 hit.
SM00243. GAS2. 1 hit.
[Graphical view]
SUPFAMiSSF143575. SSF143575. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS50021. CH. 1 hit.
PS51460. GAR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11862-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMCTALSPKV RSGPGLSDMH QYSQWLASRH EANLLPMKED LALWLTNLLG
60 70 80 90 100
KEITAETFME KLDNGALLCQ LAATVQEKFK ESMDANKPAK TLPLKKIPCK
110 120 130 140 150
ASAPSGSFFA RDNTANFLSW CRDLGVDETC LFESEGLVLH KQPREVCLCL
160 170 180 190 200
LELGRIAARY GVEPPGLIKL EKEIEQEETL SAPSPSPSPS SKSSGKKSTG
210 220 230 240 250
NLLDDAVKRI SEDPPCKCPT KFCVERLSQG RYRVGEKILF IRMLHNKHVM
260 270 280 290 300
VRVGGGWETF AGYLLKHDPC RMLQISRVDG KTSPVQSKSP TLKDMNPDNY
310
LVVSATYKAK KEIK
Length:314
Mass (Da):34,901
Last modified:October 1, 1989 - v1
Checksum:i7F2CC704B4057FAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21828 mRNA. Translation: AAA37660.1.
BC013456 mRNA. Translation: AAH13456.1.
BC053446 mRNA. Translation: AAH53446.1.
CCDSiCCDS21310.1.
PIRiA31590.
RefSeqiNP_032113.1. NM_008087.2.
XP_006540687.1. XM_006540624.2.
XP_006540688.1. XM_006540625.2.
XP_006540689.1. XM_006540626.2.
XP_006540690.1. XM_006540627.2.
UniGeneiMm.207360.
Mm.386823.
Mm.471773.

Genome annotation databases

EnsembliENSMUST00000051912; ENSMUSP00000053514; ENSMUSG00000030498.
ENSMUST00000107591; ENSMUSP00000103217; ENSMUSG00000030498.
ENSMUST00000208711; ENSMUSP00000146537; ENSMUSG00000030498.
GeneIDi14453.
KEGGimmu:14453.
UCSCiuc009hch.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21828 mRNA. Translation: AAA37660.1.
BC013456 mRNA. Translation: AAH13456.1.
BC053446 mRNA. Translation: AAH53446.1.
CCDSiCCDS21310.1.
PIRiA31590.
RefSeqiNP_032113.1. NM_008087.2.
XP_006540687.1. XM_006540624.2.
XP_006540688.1. XM_006540625.2.
XP_006540689.1. XM_006540626.2.
XP_006540690.1. XM_006540627.2.
UniGeneiMm.207360.
Mm.386823.
Mm.471773.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V5RNMR-A201-284[»]
ProteinModelPortaliP11862.
SMRiP11862. Positions 193-284.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000053514.

PTM databases

iPTMnetiP11862.
PhosphoSiteiP11862.

Proteomic databases

EPDiP11862.
MaxQBiP11862.
PaxDbiP11862.
PRIDEiP11862.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051912; ENSMUSP00000053514; ENSMUSG00000030498.
ENSMUST00000107591; ENSMUSP00000103217; ENSMUSG00000030498.
ENSMUST00000208711; ENSMUSP00000146537; ENSMUSG00000030498.
GeneIDi14453.
KEGGimmu:14453.
UCSCiuc009hch.1. mouse.

Organism-specific databases

CTDi2620.
MGIiMGI:95657. Gas2.

Phylogenomic databases

eggNOGiENOG410KD4B. Eukaryota.
ENOG4111BF1. LUCA.
GeneTreeiENSGT00390000002205.
HOGENOMiHOG000007404.
HOVERGENiHBG005805.
InParanoidiP11862.
OMAiKFKESME.
PhylomeDBiP11862.
TreeFamiTF323754.

Enzyme and pathway databases

ReactomeiR-MMU-264870. Caspase-mediated cleavage of cytoskeletal proteins.

Miscellaneous databases

ChiTaRSiGas2. mouse.
EvolutionaryTraceiP11862.
NextBioi286073.
PROiP11862.
SOURCEiSearch...

Gene expression databases

BgeeiP11862.
CleanExiMM_GAS2.
ExpressionAtlasiP11862. baseline and differential.
GenevisibleiP11862. MM.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
3.30.920.20. 1 hit.
InterProiIPR001715. CH-domain.
IPR029929. GAS2.
IPR003108. GAS2_dom.
[Graphical view]
PANTHERiPTHR11915:SF298. PTHR11915:SF298. 1 hit.
PfamiPF00307. CH. 1 hit.
PF02187. GAS2. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 1 hit.
SM00243. GAS2. 1 hit.
[Graphical view]
SUPFAMiSSF143575. SSF143575. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS50021. CH. 1 hit.
PS51460. GAR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genes specifically expressed at growth arrest of mammalian cells."
    Schneider C., King R.M., Philipson L.
    Cell 54:787-793(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Embryo and Kidney.
  3. "Gas2, a growth arrest-specific protein, is a component of the microfilament network system."
    Brancolini C., Bottega S., Schneider C.
    J. Cell Biol. 117:1251-1261(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "Phosphorylation of the growth arrest-specific protein Gas2 is coupled to actin rearrangements during Go-->G1 transition in NIH 3T3 cells."
    Brancolini C., Schneider C.
    J. Cell Biol. 124:743-756(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION, SUBCELLULAR LOCATION.
  5. "Microfilament reorganization during apoptosis: the role of Gas2, a possible substrate for ICE-like proteases."
    Brancolini C., Benedetti M., Schneider C.
    EMBO J. 14:5179-5190(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING.
  6. "gas2 is a multifunctional gene involved in the regulation of apoptosis and chondrogenesis in the developing mouse limb."
    Lee K.K., Tang M.K., Yew D.T., Chow P.H., Yee S.P., Schneider C., Brancolini C.
    Dev. Biol. 207:14-25(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.
  7. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
    Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
    J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-184 AND SER-188, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney and Liver.
  9. "Solution structure of the GAS2 domain of the growth arrest specific 2 protein."
    RIKEN structural genomics initiative (RSGI)
    Submitted (JAN-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 198-284.

Entry informationi

Entry nameiGAS2_MOUSE
AccessioniPrimary (citable) accession number: P11862
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: May 11, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-2 is the initiator.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.