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Protein

Protein phosphatase inhibitor 2

Gene

PPP1R2

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of protein-phosphatase 1.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protein phosphatase inhibitor

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase inhibitor 2
Short name:
IPP-2
Gene namesi
Name:PPP1R2
Synonyms:IPP2
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved2 Publications
Chaini2 – 205204Protein phosphatase inhibitor 2PRO_0000071483Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine2 Publications
Modified residuei44 – 441Phosphoserine; by ATMBy similarity
Modified residuei73 – 731Phosphothreonine; by GSK31 Publication
Modified residuei87 – 871Phosphoserine1 Publication
Modified residuei89 – 891PhosphothreonineBy similarity
Modified residuei92 – 921PhosphothreonineBy similarity
Modified residuei96 – 961PhosphothreonineBy similarity
Modified residuei121 – 1211Phosphoserine1 Publication
Modified residuei122 – 1221Phosphoserine1 Publication
Modified residuei127 – 1271PhosphoserineBy similarity
Modified residuei130 – 1301PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex (By similarity). Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex.By similarity3 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

PTM databases

iPTMnetiP11845.

Interactioni

Subunit structurei

Heterodimer with PP1.1 Publication

Protein-protein interaction databases

DIPiDIP-439N.
STRINGi9986.ENSOCUP00000012136.

Structurei

3D structure databases

DisProtiDP00232.
ProteinModelPortaliP11845.
SMRiP11845. Positions 127-168.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni12 – 176Required for binding PPP1CCBy similarity
Regioni43 – 5513Required for binding PPP1CCBy similarityAdd
BLAST
Regioni147 – 1504Required for binding PPP1CC catalytic center, displacing metal ions and inhibition of PPP1CC catyltic activityBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG4041. Eukaryota.
ENOG4111SYV. LUCA.
HOGENOMiHOG000017696.
HOVERGENiHBG006170.
InParanoidiP11845.
KOiK16833.
OMAiMHYNEGR.
OrthoDBiEOG776SR7.
TreeFamiTF105536.

Family and domain databases

InterProiIPR007062. PPI-2.
[Graphical view]
PfamiPF04979. IPP-2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11845-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASTASHRP IKGILKNKTS STSSRVASAE QPRGSVDEEL SKKSQKWDEM
60 70 80 90 100
NILATYHPAD KDYGLMKIDE PSTPYHSMIG DDDDAYSDTE TTEAMTPDTL
110 120 130 140 150
AKKLAAAEGS EPKYRIREQE SSGEEDSDLS PEEREKKRQF EMKRKLHYNE
160 170 180 190 200
GLNIKLARQL ISKDLHDDEE DEEMSETADG ESMNTEESNQ GSTPSDQRQN

KSQSS
Length:205
Mass (Da):23,011
Last modified:January 23, 2007 - v3
Checksum:iB82E80AAD2A0A7D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20858 mRNA. Translation: AAA17541.1.
M95581 mRNA. Translation: AAA31405.1.
PIRiI46876.
RefSeqiNP_001076213.1. NM_001082744.2.
UniGeneiOcu.1811.

Genome annotation databases

GeneIDi100009520.
KEGGiocu:100009520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20858 mRNA. Translation: AAA17541.1.
M95581 mRNA. Translation: AAA31405.1.
PIRiI46876.
RefSeqiNP_001076213.1. NM_001082744.2.
UniGeneiOcu.1811.

3D structure databases

DisProtiDP00232.
ProteinModelPortaliP11845.
SMRiP11845. Positions 127-168.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-439N.
STRINGi9986.ENSOCUP00000012136.

PTM databases

iPTMnetiP11845.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100009520.
KEGGiocu:100009520.

Organism-specific databases

CTDi5504.

Phylogenomic databases

eggNOGiKOG4041. Eukaryota.
ENOG4111SYV. LUCA.
HOGENOMiHOG000017696.
HOVERGENiHBG006170.
InParanoidiP11845.
KOiK16833.
OMAiMHYNEGR.
OrthoDBiEOG776SR7.
TreeFamiTF105536.

Family and domain databases

InterProiIPR007062. PPI-2.
[Graphical view]
PfamiPF04979. IPP-2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular mechanism of the synergistic phosphorylation of phosphatase inhibitor-2. Cloning, expression, and site-directed mutagenesis of inhibitor-2."
    Park I.-K., Roach P., Bondor J., Fox S.P., Depaoli-Roach A.A.
    J. Biol. Chem. 269:944-954(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: New Zealand white.
    Tissue: Skeletal muscle.
  2. "PCR cloning of the cDNA of rabbit skeletal muscle protein phosphatase inhibitor-2."
    Zhang Z., Bai G., Lee E.Y.C.
    Biochem. Biophys. Res. Commun. 186:1168-1170(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Skeletal muscle.
  3. "The protein phosphatases involved in cellular regulation. Primary structure of inhibitor-2 from rabbit skeletal muscle."
    Holmes C.F.B., Campbell D.G., Caudwell F.B., Aitken A., Cohen P.
    Eur. J. Biochem. 155:173-182(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-204, ACETYLATION AT ALA-2.
    Tissue: Skeletal muscle.
  4. "Amino acid sequence at the site on protein phosphatase inhibitor-2, phosphorylated by glycogen synthase kinase-3."
    Aitken A., Holmes C.F., Campbell D.G., Resink T.J., Cohen P., Leung C.T., Williams D.H.
    Biochim. Biophys. Acta 790:288-291(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 67-77, PHOSPHORYLATION AT THR-73.
  5. "Reconstitution of a Mg-ATP-dependent protein phosphatase and its activation through a phosphorylation mechanism."
    Hemmings B.A., Resink T.J., Cohen P.
    FEBS Lett. 150:319-324(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, PHOSPHORYLATION.
  6. "Analysis of the in vivo phosphorylation state of protein phosphatase inhibitor-2 from rabbit skeletal muscle by fast-atom bombardment mass spectrometry."
    Holmes C.F.B., Tonks N.K., Major H., Cohen P.
    Biochim. Biophys. Acta 929:208-219(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION AT ALA-2, PHOSPHORYLATION AT SER-87; SER-121 AND SER-122, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiIPP2_RABIT
AccessioniPrimary (citable) accession number: P11845
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: January 20, 2016
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.