P11839 (CASB_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-casein

Gene names

Name:

CSN2

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Important role in determination of the surface properties of the casein micelles.
Subcellular location	Secreted.
Tissue specificity	Mammary gland specific. Secreted in milk.
Polymorphism	The frequency of variant Val-198 is 0.237, 0.244 and 0.176 in Italian breeds Comisana, Sarda and Sopravissana, respectively.
Sequence similarities	Belongs to the beta-casein family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Milk protein
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Ref.3
	Chain	16 – 222	207	Beta-casein		PRO_0000004481
Amino acid modifications
	Modified residue	27	1	Phosphothreonine By similarity
	Modified residue	30	1	Phosphoserine By similarity
	Modified residue	32	1	Phosphoserine By similarity
	Modified residue	33	1	Phosphoserine By similarity
	Modified residue	34	1	Phosphoserine By similarity
	Modified residue	50	1	Phosphoserine By similarity
Natural variations
	Natural variant	198	1	M → V. Ref.4
Experimental info
	Sequence conflict	70	1	A → T AA sequence Ref.3
	Sequence conflict	82	1	P → A AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P11839 [UniParc]. Last modified November 1, 1995. Version 3. Checksum: 061B4424DCB49EB1	FASTA	222	24,875
	10 20 30 40 50 60 MKVLILACLV ALALAREQEE LNVVGETVES LSSSEESITH INKKIEKFQS EEQQQTEDEL 70 80 90 100 110 120 QDKIHPFAQA QSLVYPFTGP IPNSLPQNIL PLTQTPVVVP PFLQPEIMGV PKVKETMVPK 130 140 150 160 170 180 HKEMPFPKYP VEPFTESQSL TLTDVEKLHL PLPLVQSWMH QPPQPLPPTV MFPPQSVLSL 190 200 210 220 SQPKVLPVPQ KAVPQRDMPI QAFLLYQEPV LGPVRGPFPI LV

References

[1]	"Complete nucleotide sequence of ovine beta-casein cDNA: inter-species comparison." Provot C., Persuy M.A., Mercier J.-C. Biochimie 71:827-832(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequence of the ovine beta-casein-encoding gene and interspecies comparison." Provot C., Persuy M.A., Mercier J.-C. Gene 154:259-263(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The primary structure of the ovine beta-caseins." Richardson B.C., Mercier J.-C. Eur. J. Biochem. 99:285-297(1979) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-222.
[4]	"Single nucleotide polymorphisms in the ovine casein genes detected by polymerase chain reaction-single strand conformation polymorphism." Ceriotti G., Chessa S., Bolla P., Budelli E., Bianchi L., Duranti E., Caroli A. J. Dairy Sci. 87:2606-2613(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 123-221, VARIANT VAL-198. Strain: Comisana, Sarda and Sopravissana. Tissue: Blood.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X16482 mRNA. Translation: CAA34502.1. X79703 Genomic DNA. Translation: CAA56139.1. AY444504 Genomic DNA. Translation: AAS17942.1.
PIR	A32979.
RefSeq	NP_001009373.1. NM_001009373.1.
UniGene	Oar.425.
3D structure databases
ModBase	Search...
Protein family/group databases
Allergome	1241. Ovi a 8. 2964. Ovi a 11.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	443391.
Organism-specific databases
CTD	1447.
Phylogenomic databases
HOVERGEN	HBG004973.
Family and domain databases
InterPro	IPR001588. Casein. IPR016345. Casein_beta. [Graphical view]
PANTHER	PTHR11500. PTHR11500. 1 hit.
Pfam	PF00363. Casein. 1 hit. [Graphical view]
PIRSF	PIRSF002372. Beta-casein. 1 hit.
PROSITE	PS00306. CASEIN_ALPHA_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CASB_SHEEP

Accession

Primary (citable) accession number: P11839
Secondary accession number(s): Q69B25

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 76 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program