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Protein

Integrin beta-2

Gene

Itgb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi139 – 1391Calcium; via carbonyl oxygenBy similarity
Metal bindingi142 – 1421CalciumBy similarity
Metal bindingi143 – 1431CalciumBy similarity
Metal bindingi348 – 3481CalciumBy similarity

GO - Molecular functioni

  1. cell adhesion molecule binding Source: MGI
  2. glycoprotein binding Source: MGI
  3. ICAM-3 receptor activity Source: MGI
  4. metal ion binding Source: UniProtKB-KW
  5. protein kinase binding Source: MGI

GO - Biological processi

  1. activated T cell proliferation Source: MGI
  2. cell adhesion Source: MGI
  3. cell-matrix adhesion Source: MGI
  4. cellular extravasation Source: MGI
  5. heterotypic cell-cell adhesion Source: MGI
  6. integrin-mediated signaling pathway Source: MGI
  7. leukocyte cell-cell adhesion Source: MGI
  8. multicellular organismal development Source: InterPro
  9. neutrophil chemotaxis Source: MGI
  10. receptor clustering Source: MGI
  11. regulation of peptidyl-tyrosine phosphorylation Source: MGI
  12. single organismal cell-cell adhesion Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Integrin, Receptor

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin beta-2
Alternative name(s):
Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta
Complement receptor C3 subunit beta
CD_antigen: CD18
Gene namesi
Name:Itgb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:96611. Itgb2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 702679ExtracellularSequence AnalysisAdd
BLAST
Transmembranei703 – 72523HelicalSequence AnalysisAdd
BLAST
Topological domaini726 – 77146CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: MGI
  2. extracellular vesicular exosome Source: MGI
  3. integrin alphaL-beta2 complex Source: MGI
  4. membrane Source: MGI
  5. membrane raft Source: MGI
  6. receptor complex Source: MGI
  7. vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Add
BLAST
Chaini24 – 771748Integrin beta-2PRO_0000016342Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei24 – 241Pyrrolidone carboxylic acidBy similarity
Disulfide bondi26 ↔ 44By similarity
Disulfide bondi34 ↔ 448By similarity
Disulfide bondi37 ↔ 63By similarity
Disulfide bondi47 ↔ 74By similarity
Glycosylationi51 – 511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi117 – 1171N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi192 ↔ 199By similarity
Disulfide bondi247 ↔ 287By similarity
Glycosylationi255 – 2551N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi387 ↔ 401By similarity
Disulfide bondi421 ↔ 446By similarity
Disulfide bondi450 ↔ 468By similarity
Disulfide bondi460 ↔ 471By similarity
Disulfide bondi473 ↔ 482By similarity
Disulfide bondi484 ↔ 515By similarity
Disulfide bondi498 ↔ 513By similarity
Glycosylationi502 – 5021N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi507 ↔ 518By similarity
Disulfide bondi520 ↔ 535By similarity
Disulfide bondi537 ↔ 560By similarity
Disulfide bondi542 ↔ 558By similarity
Disulfide bondi550 ↔ 563By similarity
Disulfide bondi565 ↔ 574By similarity
Disulfide bondi576 ↔ 599By similarity
Disulfide bondi583 ↔ 597By similarity
Disulfide bondi591 ↔ 602By similarity
Disulfide bondi604 ↔ 613By similarity
Disulfide bondi616 ↔ 619By similarity
Disulfide bondi623 ↔ 664By similarity
Glycosylationi626 – 6261N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi630 ↔ 649By similarity
Disulfide bondi633 ↔ 645By similarity
Glycosylationi644 – 6441N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi672 ↔ 697By similarity
Modified residuei747 – 7471PhosphoserineBy similarity
Modified residuei758 – 7581PhosphoserineBy similarity
Modified residuei760 – 7601PhosphothreonineBy similarity
Modified residuei762 – 7621PhosphothreonineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Pyrrolidone carboxylic acid

Proteomic databases

MaxQBiP11835.
PaxDbiP11835.
PRIDEiP11835.

PTM databases

PhosphoSiteiP11835.

Expressioni

Gene expression databases

CleanExiMM_ITGB2.
GenevestigatoriP11835.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Beta-2 associates with either alpha-L, alpha-M, alpha-X or alpha-D. Interacts with COPS5 and RANBP9 (By similarity). Interacts with FGR.By similarity1 Publication

Protein-protein interaction databases

BioGridi200828. 1 interaction.
DIPiDIP-37428N.
IntActiP11835. 6 interactions.
MINTiMINT-1662243.

Structurei

3D structure databases

ProteinModelPortaliP11835.
SMRiP11835. Positions 24-698.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini125 – 364240VWFAAdd
BLAST
Repeati450 – 49748IAdd
BLAST
Repeati498 – 54144IIAdd
BLAST
Repeati542 – 58241IIIAdd
BLAST
Repeati583 – 61836IVAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni450 – 618169Cysteine-rich tandem repeatsAdd
BLAST

Sequence similaritiesi

Belongs to the integrin beta chain family.Curated
Contains 1 VWFA domain.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG287997.
HOGENOMiHOG000252936.
HOVERGENiHBG006190.
InParanoidiP11835.
KOiK06464.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR015812. Integrin_bsu.
IPR015439. Integrin_bsu-2.
IPR014836. Integrin_bsu_cyt_dom.
IPR002369. Integrin_bsu_N.
IPR012896. Integrin_bsu_tail.
IPR016201. Plexin-like_fold.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10082. PTHR10082. 1 hit.
PTHR10082:SF15. PTHR10082:SF15. 1 hit.
PfamiPF08725. Integrin_b_cyt. 1 hit.
PF07965. Integrin_B_tail. 1 hit.
PF00362. Integrin_beta. 1 hit.
[Graphical view]
PIRSFiPIRSF002512. Integrin_B. 1 hit.
PRINTSiPR01186. INTEGRINB.
SMARTiSM00187. INB. 1 hit.
SM00423. PSI. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF103575. SSF103575. 1 hit.
SSF53300. SSF53300. 1 hit.
SSF69687. SSF69687. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 3 hits.
PS00243. INTEGRIN_BETA. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11835-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLGLRPSLLL ALAGLFFLGS AVSQECTKYK VSSCRDCIQS GPGCSWCQKL
60 70 80 90 100
NFTGPGEPDS LRCDTRAQLL LKGCPADDIM DPRSIANPEF DQRGQRKQLS
110 120 130 140 150
PQKVTLYLRP GQAAAFNVTF RRAKGYPIDL YYLMDLSYSM LDDLNNVKKL
160 170 180 190 200
GGDLLQALNE ITESGRIGFG SFVDKTVLPF VNTHPEKLRN PCPNKEKACQ
210 220 230 240 250
PPFAFRHVLK LTDNSNQFQT EVGKQLISGN LDAPEGGLDA IMQVAACPEE
260 270 280 290 300
IGWRNVTRLL VFATDDGFHF AGDGKLGAIL TPNDGRCHLE DNMYKRSNEF
310 320 330 340 350
DYPSVGQLAH KLSESNIQPI FAVTKKMVKT YEKLTEIIPK SAVGELSDDS
360 370 380 390 400
SNVVQLIKNA YYKLSSRVFL DHSTLPDTLK VTYDSFCSNG ASSIGKSRGD
410 420 430 440 450
CDGVQINNPV TFQVKVMASE CIQEQSFVIR ALGFTDTVTV QVRPQCECQC
460 470 480 490 500
RDQSREQSLC GGKGVMECGI CRCESGYIGK NCECQTQGRS SQELERNCRK
510 520 530 540 550
DNSSIVCSGL GDCICGQCVC HTSDVPNKEI FGQYCECDNV NCERYNSQVC
560 570 580 590 600
GGSDRGSCNC GKCSCKPGYE GSACQCQRST TGCLNARLVE CSGRGHCQCN
610 620 630 640 650
RCICDEGYQP PMCEDCPSCG SHCRDNHTSC AECLKFDKGP FEKNCSVQCA
660 670 680 690 700
GMTLQTIPLK KKPCKERDSE GCWITYTLQQ KDGRNIYNIH VEDSLECVKG
710 720 730 740 750
PNVAAIVGGT VVGVVLIGVL LLVIWKALTH LTDLREYRRF EKEKLKSQWN
760 770
NDNPLFKSAT TTVMNPKFAE S
Length:771
Mass (Da):85,026
Last modified:July 15, 1998 - v2
Checksum:i6FA51DA93AB40D8A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 63LRP → PH in CAA33077. (PubMed:2569711)Curated
Sequence conflicti449 – 4491Q → H in AAA39325. (PubMed:1969385)Curated
Sequence conflicti667 – 6671R → K in AAA39325. (PubMed:1969385)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14951 mRNA. Translation: CAA33077.1.
M31039 mRNA. Translation: AAA39325.1.
PIRiA45839.
S04847.
RefSeqiNP_032430.2. NM_008404.4.
UniGeneiMm.1137.

Genome annotation databases

GeneIDi16414.
KEGGimmu:16414.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14951 mRNA. Translation: CAA33077.1.
M31039 mRNA. Translation: AAA39325.1.
PIRiA45839.
S04847.
RefSeqiNP_032430.2. NM_008404.4.
UniGeneiMm.1137.

3D structure databases

ProteinModelPortaliP11835.
SMRiP11835. Positions 24-698.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200828. 1 interaction.
DIPiDIP-37428N.
IntActiP11835. 6 interactions.
MINTiMINT-1662243.

PTM databases

PhosphoSiteiP11835.

Proteomic databases

MaxQBiP11835.
PaxDbiP11835.
PRIDEiP11835.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi16414.
KEGGimmu:16414.

Organism-specific databases

CTDi3689.
MGIiMGI:96611. Itgb2.

Phylogenomic databases

eggNOGiNOG287997.
HOGENOMiHOG000252936.
HOVERGENiHBG006190.
InParanoidiP11835.
KOiK06464.

Miscellaneous databases

NextBioi289605.
PROiP11835.
SOURCEiSearch...

Gene expression databases

CleanExiMM_ITGB2.
GenevestigatoriP11835.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR015812. Integrin_bsu.
IPR015439. Integrin_bsu-2.
IPR014836. Integrin_bsu_cyt_dom.
IPR002369. Integrin_bsu_N.
IPR012896. Integrin_bsu_tail.
IPR016201. Plexin-like_fold.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10082. PTHR10082. 1 hit.
PTHR10082:SF15. PTHR10082:SF15. 1 hit.
PfamiPF08725. Integrin_b_cyt. 1 hit.
PF07965. Integrin_B_tail. 1 hit.
PF00362. Integrin_beta. 1 hit.
[Graphical view]
PIRSFiPIRSF002512. Integrin_B. 1 hit.
PRINTSiPR01186. INTEGRINB.
SMARTiSM00187. INB. 1 hit.
SM00423. PSI. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF103575. SSF103575. 1 hit.
SSF53300. SSF53300. 1 hit.
SSF69687. SSF69687. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 3 hits.
PS00243. INTEGRIN_BETA. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the cDNA from the mouse leukocyte adhesion protein CD18."
    Wilson R.W., O'Brien W.E., Beaudet A.L.
    Nucleic Acids Res. 17:5397-5397(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X CBA.
    Tissue: Thymus.
  2. "Mouse macrophage beta subunit (CD11b) cDNA for the CR3 complement receptor/Mac-1 antigen."
    Zeger D.L., Osman N., Hennings M., McKenzie I.F., Sears D.W., Hogarth P.M.
    Immunogenetics 31:191-197(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Mouse macrophage beta subunit (CD11b) cDNA for the CR3 complement receptor/Mac-1 antigen."
    Kofler R.
    Immunogenetics 33:77-77(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO N-TERMINUS.
  4. "Nonmuscle myosin light-chain kinase mediates neutrophil transmigration in sepsis-induced lung inflammation by activating beta2 integrins."
    Xu J., Gao X.-P., Ramchandran R., Zhao Y.-Y., Vogel S.M., Malik A.B.
    Nat. Immunol. 9:880-886(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION DURING LUNG INJURY.
  5. "c-Abl and Src-family kinases cross-talk in regulation of myeloid cell migration."
    Baruzzi A., Iacobucci I., Soverini S., Lowell C.A., Martinelli G., Berton G.
    FEBS Lett. 584:15-21(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH FGR.

Entry informationi

Entry nameiITB2_MOUSE
AccessioniPrimary (citable) accession number: P11835
Secondary accession number(s): Q64482
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 15, 1998
Last modified: February 4, 2015
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.