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P11835

- ITB2_MOUSE

UniProt

P11835 - ITB2_MOUSE

Protein

Integrin beta-2

Gene

Itgb2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 148 (01 Oct 2014)
      Sequence version 2 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi139 – 1391Calcium; via carbonyl oxygenBy similarity
    Metal bindingi142 – 1421CalciumBy similarity
    Metal bindingi143 – 1431CalciumBy similarity
    Metal bindingi348 – 3481CalciumBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: UniProtKB
    3. receptor activity Source: InterPro

    GO - Biological processi

    1. activated T cell proliferation Source: MGI
    2. cell adhesion Source: MGI
    3. cell-matrix adhesion Source: InterPro
    4. cellular extravasation Source: MGI
    5. integrin-mediated signaling pathway Source: MGI
    6. multicellular organismal development Source: InterPro
    7. neutrophil chemotaxis Source: MGI
    8. single organismal cell-cell adhesion Source: MGI

    Keywords - Molecular functioni

    Integrin, Receptor

    Keywords - Biological processi

    Cell adhesion

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Integrin beta-2
    Alternative name(s):
    Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta
    Complement receptor C3 subunit beta
    CD_antigen: CD18
    Gene namesi
    Name:Itgb2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:96611. Itgb2.

    Subcellular locationi

    GO - Cellular componenti

    1. cell surface Source: MGI
    2. integrin complex Source: InterPro
    3. membrane Source: MGI
    4. membrane raft Source: MGI
    5. receptor complex Source: MGI

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Add
    BLAST
    Chaini24 – 771748Integrin beta-2PRO_0000016342Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei24 – 241Pyrrolidone carboxylic acidBy similarity
    Disulfide bondi26 ↔ 44By similarity
    Disulfide bondi34 ↔ 448By similarity
    Disulfide bondi37 ↔ 63By similarity
    Disulfide bondi47 ↔ 74By similarity
    Glycosylationi51 – 511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi117 – 1171N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi192 ↔ 199By similarity
    Disulfide bondi247 ↔ 287By similarity
    Glycosylationi255 – 2551N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi387 ↔ 401By similarity
    Disulfide bondi421 ↔ 446By similarity
    Disulfide bondi450 ↔ 468By similarity
    Disulfide bondi460 ↔ 471By similarity
    Disulfide bondi473 ↔ 482By similarity
    Disulfide bondi484 ↔ 515By similarity
    Disulfide bondi498 ↔ 513By similarity
    Glycosylationi502 – 5021N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi507 ↔ 518By similarity
    Disulfide bondi520 ↔ 535By similarity
    Disulfide bondi537 ↔ 560By similarity
    Disulfide bondi542 ↔ 558By similarity
    Disulfide bondi550 ↔ 563By similarity
    Disulfide bondi565 ↔ 574By similarity
    Disulfide bondi576 ↔ 599By similarity
    Disulfide bondi583 ↔ 597By similarity
    Disulfide bondi591 ↔ 602By similarity
    Disulfide bondi604 ↔ 613By similarity
    Disulfide bondi616 ↔ 619By similarity
    Disulfide bondi623 ↔ 664By similarity
    Glycosylationi626 – 6261N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi630 ↔ 649By similarity
    Disulfide bondi633 ↔ 645By similarity
    Glycosylationi644 – 6441N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi672 ↔ 697By similarity
    Modified residuei747 – 7471PhosphoserineBy similarity
    Modified residuei758 – 7581PhosphoserineBy similarity
    Modified residuei760 – 7601PhosphothreonineBy similarity
    Modified residuei762 – 7621PhosphothreonineBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein, Pyrrolidone carboxylic acid

    Proteomic databases

    MaxQBiP11835.
    PaxDbiP11835.
    PRIDEiP11835.

    PTM databases

    PhosphoSiteiP11835.

    Expressioni

    Gene expression databases

    CleanExiMM_ITGB2.
    GenevestigatoriP11835.

    Interactioni

    Subunit structurei

    Heterodimer of an alpha and a beta subunit. Beta-2 associates with either alpha-L, alpha-M, alpha-X or alpha-D. Interacts with COPS5 and RANBP9 By similarity. Interacts with FGR.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi200828. 1 interaction.
    DIPiDIP-37428N.
    IntActiP11835. 6 interactions.
    MINTiMINT-1662243.

    Structurei

    3D structure databases

    ProteinModelPortaliP11835.
    SMRiP11835. Positions 24-698.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 702679ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini726 – 77146CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei703 – 72523HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini125 – 364240VWFAAdd
    BLAST
    Repeati450 – 49748IAdd
    BLAST
    Repeati498 – 54144IIAdd
    BLAST
    Repeati542 – 58241IIIAdd
    BLAST
    Repeati583 – 61836IVAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni450 – 618169Cysteine-rich tandem repeatsAdd
    BLAST

    Sequence similaritiesi

    Belongs to the integrin beta chain family.Curated
    Contains 1 VWFA domain.Curated

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG287997.
    HOGENOMiHOG000252936.
    HOVERGENiHBG006190.
    InParanoidiP11835.
    KOiK06464.

    Family and domain databases

    Gene3Di3.40.50.410. 1 hit.
    InterProiIPR015812. Integrin_bsu.
    IPR015439. Integrin_bsu-2.
    IPR014836. Integrin_bsu_cyt_dom.
    IPR002369. Integrin_bsu_N.
    IPR012896. Integrin_bsu_tail.
    IPR016201. Plexin-like_fold.
    IPR002035. VWF_A.
    [Graphical view]
    PANTHERiPTHR10082. PTHR10082. 1 hit.
    PTHR10082:SF15. PTHR10082:SF15. 1 hit.
    PfamiPF08725. Integrin_b_cyt. 1 hit.
    PF07965. Integrin_B_tail. 1 hit.
    PF00362. Integrin_beta. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002512. Integrin_B. 1 hit.
    PRINTSiPR01186. INTEGRINB.
    SMARTiSM00187. INB. 1 hit.
    SM00423. PSI. 1 hit.
    SM00327. VWA. 1 hit.
    [Graphical view]
    SUPFAMiSSF103575. SSF103575. 1 hit.
    SSF53300. SSF53300. 1 hit.
    SSF69687. SSF69687. 1 hit.
    PROSITEiPS00022. EGF_1. 2 hits.
    PS01186. EGF_2. 3 hits.
    PS00243. INTEGRIN_BETA. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P11835-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLGLRPSLLL ALAGLFFLGS AVSQECTKYK VSSCRDCIQS GPGCSWCQKL    50
    NFTGPGEPDS LRCDTRAQLL LKGCPADDIM DPRSIANPEF DQRGQRKQLS 100
    PQKVTLYLRP GQAAAFNVTF RRAKGYPIDL YYLMDLSYSM LDDLNNVKKL 150
    GGDLLQALNE ITESGRIGFG SFVDKTVLPF VNTHPEKLRN PCPNKEKACQ 200
    PPFAFRHVLK LTDNSNQFQT EVGKQLISGN LDAPEGGLDA IMQVAACPEE 250
    IGWRNVTRLL VFATDDGFHF AGDGKLGAIL TPNDGRCHLE DNMYKRSNEF 300
    DYPSVGQLAH KLSESNIQPI FAVTKKMVKT YEKLTEIIPK SAVGELSDDS 350
    SNVVQLIKNA YYKLSSRVFL DHSTLPDTLK VTYDSFCSNG ASSIGKSRGD 400
    CDGVQINNPV TFQVKVMASE CIQEQSFVIR ALGFTDTVTV QVRPQCECQC 450
    RDQSREQSLC GGKGVMECGI CRCESGYIGK NCECQTQGRS SQELERNCRK 500
    DNSSIVCSGL GDCICGQCVC HTSDVPNKEI FGQYCECDNV NCERYNSQVC 550
    GGSDRGSCNC GKCSCKPGYE GSACQCQRST TGCLNARLVE CSGRGHCQCN 600
    RCICDEGYQP PMCEDCPSCG SHCRDNHTSC AECLKFDKGP FEKNCSVQCA 650
    GMTLQTIPLK KKPCKERDSE GCWITYTLQQ KDGRNIYNIH VEDSLECVKG 700
    PNVAAIVGGT VVGVVLIGVL LLVIWKALTH LTDLREYRRF EKEKLKSQWN 750
    NDNPLFKSAT TTVMNPKFAE S 771
    Length:771
    Mass (Da):85,026
    Last modified:July 15, 1998 - v2
    Checksum:i6FA51DA93AB40D8A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 63LRP → PH in CAA33077. (PubMed:2569711)Curated
    Sequence conflicti449 – 4491Q → H in AAA39325. (PubMed:1969385)Curated
    Sequence conflicti667 – 6671R → K in AAA39325. (PubMed:1969385)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14951 mRNA. Translation: CAA33077.1.
    M31039 mRNA. Translation: AAA39325.1.
    PIRiA45839.
    S04847.
    RefSeqiNP_032430.2. NM_008404.4.
    UniGeneiMm.1137.

    Genome annotation databases

    GeneIDi16414.
    KEGGimmu:16414.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14951 mRNA. Translation: CAA33077.1 .
    M31039 mRNA. Translation: AAA39325.1 .
    PIRi A45839.
    S04847.
    RefSeqi NP_032430.2. NM_008404.4.
    UniGenei Mm.1137.

    3D structure databases

    ProteinModelPortali P11835.
    SMRi P11835. Positions 24-698.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200828. 1 interaction.
    DIPi DIP-37428N.
    IntActi P11835. 6 interactions.
    MINTi MINT-1662243.

    Chemistry

    BindingDBi P11835.

    PTM databases

    PhosphoSitei P11835.

    Proteomic databases

    MaxQBi P11835.
    PaxDbi P11835.
    PRIDEi P11835.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 16414.
    KEGGi mmu:16414.

    Organism-specific databases

    CTDi 3689.
    MGIi MGI:96611. Itgb2.

    Phylogenomic databases

    eggNOGi NOG287997.
    HOGENOMi HOG000252936.
    HOVERGENi HBG006190.
    InParanoidi P11835.
    KOi K06464.

    Miscellaneous databases

    NextBioi 289605.
    PROi P11835.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_ITGB2.
    Genevestigatori P11835.

    Family and domain databases

    Gene3Di 3.40.50.410. 1 hit.
    InterProi IPR015812. Integrin_bsu.
    IPR015439. Integrin_bsu-2.
    IPR014836. Integrin_bsu_cyt_dom.
    IPR002369. Integrin_bsu_N.
    IPR012896. Integrin_bsu_tail.
    IPR016201. Plexin-like_fold.
    IPR002035. VWF_A.
    [Graphical view ]
    PANTHERi PTHR10082. PTHR10082. 1 hit.
    PTHR10082:SF15. PTHR10082:SF15. 1 hit.
    Pfami PF08725. Integrin_b_cyt. 1 hit.
    PF07965. Integrin_B_tail. 1 hit.
    PF00362. Integrin_beta. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002512. Integrin_B. 1 hit.
    PRINTSi PR01186. INTEGRINB.
    SMARTi SM00187. INB. 1 hit.
    SM00423. PSI. 1 hit.
    SM00327. VWA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103575. SSF103575. 1 hit.
    SSF53300. SSF53300. 1 hit.
    SSF69687. SSF69687. 1 hit.
    PROSITEi PS00022. EGF_1. 2 hits.
    PS01186. EGF_2. 3 hits.
    PS00243. INTEGRIN_BETA. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the cDNA from the mouse leukocyte adhesion protein CD18."
      Wilson R.W., O'Brien W.E., Beaudet A.L.
      Nucleic Acids Res. 17:5397-5397(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6 X CBA.
      Tissue: Thymus.
    2. "Mouse macrophage beta subunit (CD11b) cDNA for the CR3 complement receptor/Mac-1 antigen."
      Zeger D.L., Osman N., Hennings M., McKenzie I.F., Sears D.W., Hogarth P.M.
      Immunogenetics 31:191-197(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Mouse macrophage beta subunit (CD11b) cDNA for the CR3 complement receptor/Mac-1 antigen."
      Kofler R.
      Immunogenetics 33:77-77(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO N-TERMINUS.
    4. "Nonmuscle myosin light-chain kinase mediates neutrophil transmigration in sepsis-induced lung inflammation by activating beta2 integrins."
      Xu J., Gao X.-P., Ramchandran R., Zhao Y.-Y., Vogel S.M., Malik A.B.
      Nat. Immunol. 9:880-886(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION DURING LUNG INJURY.
    5. "c-Abl and Src-family kinases cross-talk in regulation of myeloid cell migration."
      Baruzzi A., Iacobucci I., Soverini S., Lowell C.A., Martinelli G., Berton G.
      FEBS Lett. 584:15-21(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH FGR.

    Entry informationi

    Entry nameiITB2_MOUSE
    AccessioniPrimary (citable) accession number: P11835
    Secondary accession number(s): Q64482
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 148 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3