Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Integrin beta-2

Gene

Itgb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity (By similarity). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (By similarity). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed:18587400). Integrin alpha-L/beta-2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (By similarity).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi139Calcium; via carbonyl oxygenBy similarity1
Metal bindingi142CalciumBy similarity1
Metal bindingi143CalciumBy similarity1
Metal bindingi348CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • activated T cell proliferation Source: MGI
  • cell adhesion Source: MGI
  • cell-matrix adhesion Source: MGI
  • cellular extravasation Source: MGI
  • cellular response to low-density lipoprotein particle stimulus Source: UniProtKB
  • heterotypic cell-cell adhesion Source: MGI
  • integrin-mediated signaling pathway Source: MGI
  • leukocyte cell-cell adhesion Source: MGI
  • neutrophil chemotaxis Source: MGI
  • phagocytosis Source: UniProtKB-KW
  • receptor clustering Source: MGI
  • receptor internalization Source: UniProtKB
  • regulation of peptidyl-tyrosine phosphorylation Source: MGI
  • single organismal cell-cell adhesion Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Integrin, Receptor

Keywords - Biological processi

Cell adhesion, Phagocytosis

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin beta-2
Alternative name(s):
Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta
Complement receptor C3 subunit beta
CD_antigen: CD18
Gene namesi
Name:Itgb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96611. Itgb2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 702ExtracellularSequence analysisAdd BLAST679
Transmembranei703 – 725HelicalSequence analysisAdd BLAST23
Topological domaini726 – 771CytoplasmicSequence analysisAdd BLAST46

GO - Cellular componenti

  • cell surface Source: MGI
  • extracellular exosome Source: MGI
  • extracellular vesicle Source: MGI
  • integrin alphaL-beta2 complex Source: MGI
  • membrane Source: UniProtKB
  • membrane raft Source: MGI
  • receptor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Add BLAST23
ChainiPRO_000001634224 – 771Integrin beta-2Add BLAST748

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 44By similarity
Disulfide bondi34 ↔ 448By similarity
Disulfide bondi37 ↔ 63By similarity
Disulfide bondi47 ↔ 74By similarity
Glycosylationi51N-linked (GlcNAc...)Sequence analysis1
Glycosylationi117N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi192 ↔ 199By similarity
Disulfide bondi247 ↔ 287By similarity
Glycosylationi255N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi387 ↔ 401By similarity
Disulfide bondi421 ↔ 446By similarity
Disulfide bondi450 ↔ 468By similarity
Disulfide bondi460 ↔ 471By similarity
Disulfide bondi473 ↔ 482By similarity
Disulfide bondi484 ↔ 515By similarity
Disulfide bondi498 ↔ 513By similarity
Glycosylationi502N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi507 ↔ 518By similarity
Disulfide bondi520 ↔ 535By similarity
Disulfide bondi537 ↔ 560By similarity
Disulfide bondi542 ↔ 558By similarity
Disulfide bondi550 ↔ 563By similarity
Disulfide bondi565 ↔ 574By similarity
Disulfide bondi576 ↔ 599By similarity
Disulfide bondi583 ↔ 597By similarity
Disulfide bondi591 ↔ 602By similarity
Disulfide bondi604 ↔ 613By similarity
Disulfide bondi616 ↔ 619By similarity
Disulfide bondi623 ↔ 664By similarity
Glycosylationi626N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi630 ↔ 649By similarity
Disulfide bondi633 ↔ 645By similarity
Glycosylationi644N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi672 ↔ 697By similarity
Modified residuei747PhosphoserineBy similarity1
Modified residuei758PhosphoserineBy similarity1
Modified residuei760PhosphothreonineBy similarity1
Modified residuei762PhosphothreonineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP11835.
MaxQBiP11835.
PaxDbiP11835.
PeptideAtlasiP11835.
PRIDEiP11835.

PTM databases

iPTMnetiP11835.
PhosphoSitePlusiP11835.
SwissPalmiP11835.

Expressioni

Gene expression databases

CleanExiMM_ITGB2.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Beta-2 associates with either alpha-L, alpha-M, alpha-X or alpha-D. Interacts with COPS5 and RANBP9 (By similarity). Interacts with FGR (PubMed:19903482). Interacts with FLNA (via filamin repeats 4, 9, 12, 17, 19, 21, and 23) (By similarity). Interacts with THBD (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200828. 1 interactor.
DIPiDIP-37428N.
IntActiP11835. 6 interactors.
MINTiMINT-1662243.
STRINGi10090.ENSMUSP00000000299.

Structurei

3D structure databases

ProteinModelPortaliP11835.
SMRiP11835.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini125 – 364VWFAAdd BLAST240
Repeati450 – 497IAdd BLAST48
Repeati498 – 541IIAdd BLAST44
Repeati542 – 582IIIAdd BLAST41
Repeati583 – 618IVAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni450 – 618Cysteine-rich tandem repeatsAdd BLAST169

Sequence similaritiesi

Belongs to the integrin beta chain family.Curated
Contains 1 VWFA domain.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1226. Eukaryota.
ENOG410XP60. LUCA.
HOGENOMiHOG000252936.
HOVERGENiHBG006190.
InParanoidiP11835.
KOiK06464.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR033760. Integin_beta_N.
IPR015812. Integrin_bsu.
IPR015439. Integrin_bsu-2.
IPR014836. Integrin_bsu_cyt_dom.
IPR012896. Integrin_bsu_tail.
IPR002369. Integrin_bsu_VWA.
IPR032695. Integrin_dom.
IPR016201. PSI.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10082. PTHR10082. 1 hit.
PTHR10082:SF15. PTHR10082:SF15. 1 hit.
PfamiPF08725. Integrin_b_cyt. 1 hit.
PF07965. Integrin_B_tail. 1 hit.
PF00362. Integrin_beta. 1 hit.
PF17205. PSI_integrin. 1 hit.
[Graphical view]
PIRSFiPIRSF002512. Integrin_B. 1 hit.
PRINTSiPR01186. INTEGRINB.
SMARTiSM00187. INB. 1 hit.
SM01241. Integrin_b_cyt. 1 hit.
SM01242. Integrin_B_tail. 1 hit.
SM00423. PSI. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF69179. SSF69179. 1 hit.
SSF69687. SSF69687. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 3 hits.
PS00243. INTEGRIN_BETA. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11835-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGLRPSLLL ALAGLFFLGS AVSQECTKYK VSSCRDCIQS GPGCSWCQKL
60 70 80 90 100
NFTGPGEPDS LRCDTRAQLL LKGCPADDIM DPRSIANPEF DQRGQRKQLS
110 120 130 140 150
PQKVTLYLRP GQAAAFNVTF RRAKGYPIDL YYLMDLSYSM LDDLNNVKKL
160 170 180 190 200
GGDLLQALNE ITESGRIGFG SFVDKTVLPF VNTHPEKLRN PCPNKEKACQ
210 220 230 240 250
PPFAFRHVLK LTDNSNQFQT EVGKQLISGN LDAPEGGLDA IMQVAACPEE
260 270 280 290 300
IGWRNVTRLL VFATDDGFHF AGDGKLGAIL TPNDGRCHLE DNMYKRSNEF
310 320 330 340 350
DYPSVGQLAH KLSESNIQPI FAVTKKMVKT YEKLTEIIPK SAVGELSDDS
360 370 380 390 400
SNVVQLIKNA YYKLSSRVFL DHSTLPDTLK VTYDSFCSNG ASSIGKSRGD
410 420 430 440 450
CDGVQINNPV TFQVKVMASE CIQEQSFVIR ALGFTDTVTV QVRPQCECQC
460 470 480 490 500
RDQSREQSLC GGKGVMECGI CRCESGYIGK NCECQTQGRS SQELERNCRK
510 520 530 540 550
DNSSIVCSGL GDCICGQCVC HTSDVPNKEI FGQYCECDNV NCERYNSQVC
560 570 580 590 600
GGSDRGSCNC GKCSCKPGYE GSACQCQRST TGCLNARLVE CSGRGHCQCN
610 620 630 640 650
RCICDEGYQP PMCEDCPSCG SHCRDNHTSC AECLKFDKGP FEKNCSVQCA
660 670 680 690 700
GMTLQTIPLK KKPCKERDSE GCWITYTLQQ KDGRNIYNIH VEDSLECVKG
710 720 730 740 750
PNVAAIVGGT VVGVVLIGVL LLVIWKALTH LTDLREYRRF EKEKLKSQWN
760 770
NDNPLFKSAT TTVMNPKFAE S
Length:771
Mass (Da):85,026
Last modified:July 15, 1998 - v2
Checksum:i6FA51DA93AB40D8A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4 – 6LRP → PH in CAA33077 (PubMed:2569711).Curated3
Sequence conflicti449Q → H in AAA39325 (PubMed:1969385).Curated1
Sequence conflicti667R → K in AAA39325 (PubMed:1969385).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14951 mRNA. Translation: CAA33077.1.
M31039 mRNA. Translation: AAA39325.1.
PIRiA45839.
S04847.
RefSeqiNP_032430.2. NM_008404.4.
UniGeneiMm.1137.

Genome annotation databases

GeneIDi16414.
KEGGimmu:16414.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14951 mRNA. Translation: CAA33077.1.
M31039 mRNA. Translation: AAA39325.1.
PIRiA45839.
S04847.
RefSeqiNP_032430.2. NM_008404.4.
UniGeneiMm.1137.

3D structure databases

ProteinModelPortaliP11835.
SMRiP11835.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200828. 1 interactor.
DIPiDIP-37428N.
IntActiP11835. 6 interactors.
MINTiMINT-1662243.
STRINGi10090.ENSMUSP00000000299.

PTM databases

iPTMnetiP11835.
PhosphoSitePlusiP11835.
SwissPalmiP11835.

Proteomic databases

EPDiP11835.
MaxQBiP11835.
PaxDbiP11835.
PeptideAtlasiP11835.
PRIDEiP11835.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi16414.
KEGGimmu:16414.

Organism-specific databases

CTDi3689.
MGIiMGI:96611. Itgb2.

Phylogenomic databases

eggNOGiKOG1226. Eukaryota.
ENOG410XP60. LUCA.
HOGENOMiHOG000252936.
HOVERGENiHBG006190.
InParanoidiP11835.
KOiK06464.

Miscellaneous databases

PROiP11835.
SOURCEiSearch...

Gene expression databases

CleanExiMM_ITGB2.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR033760. Integin_beta_N.
IPR015812. Integrin_bsu.
IPR015439. Integrin_bsu-2.
IPR014836. Integrin_bsu_cyt_dom.
IPR012896. Integrin_bsu_tail.
IPR002369. Integrin_bsu_VWA.
IPR032695. Integrin_dom.
IPR016201. PSI.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10082. PTHR10082. 1 hit.
PTHR10082:SF15. PTHR10082:SF15. 1 hit.
PfamiPF08725. Integrin_b_cyt. 1 hit.
PF07965. Integrin_B_tail. 1 hit.
PF00362. Integrin_beta. 1 hit.
PF17205. PSI_integrin. 1 hit.
[Graphical view]
PIRSFiPIRSF002512. Integrin_B. 1 hit.
PRINTSiPR01186. INTEGRINB.
SMARTiSM00187. INB. 1 hit.
SM01241. Integrin_b_cyt. 1 hit.
SM01242. Integrin_B_tail. 1 hit.
SM00423. PSI. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF69179. SSF69179. 1 hit.
SSF69687. SSF69687. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 3 hits.
PS00243. INTEGRIN_BETA. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiITB2_MOUSE
AccessioniPrimary (citable) accession number: P11835
Secondary accession number(s): Q64482
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 15, 1998
Last modified: November 30, 2016
This is version 168 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.