Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P11835 (ITB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 147. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Integrin beta-2
Alternative name(s):
Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta
Complement receptor C3 subunit beta
CD_antigen=CD18
Gene names
Name:Itgb2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length771 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. Ref.4 Ref.5

Subunit structure

Heterodimer of an alpha and a beta subunit. Beta-2 associates with either alpha-L, alpha-M, alpha-X or alpha-D. Interacts with COPS5 and RANBP9 By similarity. Interacts with FGR. Ref.5

Subcellular location

Membrane; Single-pass type I membrane protein.

Sequence similarities

Belongs to the integrin beta chain family.

Contains 1 VWFA domain.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentMembrane
   DomainRepeat
Signal
Transmembrane
Transmembrane helix
   LigandCalcium
Metal-binding
   Molecular functionIntegrin
Receptor
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
Pyrrolidone carboxylic acid
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivated T cell proliferation

Inferred from mutant phenotype PubMed 15210787. Source: MGI

cell adhesion

Inferred from mutant phenotype PubMed 10528208. Source: MGI

cell-matrix adhesion

Inferred from electronic annotation. Source: InterPro

cellular extravasation

Inferred from mutant phenotype PubMed 10528208. Source: MGI

integrin-mediated signaling pathway

Inferred from direct assay PubMed 15249579. Source: MGI

multicellular organismal development

Inferred from electronic annotation. Source: InterPro

neutrophil chemotaxis

Inferred from mutant phenotype PubMed 10528208. Source: MGI

single organismal cell-cell adhesion

Inferred from genetic interaction PubMed 16093349. Source: MGI

   Cellular_componentcell surface

Inferred from direct assay PubMed 17023661. Source: MGI

integrin complex

Inferred from electronic annotation. Source: InterPro

membrane

Inferred from direct assay PubMed 10601245. Source: MGI

membrane raft

Inferred from direct assay PubMed 10601245. Source: MGI

receptor complex

Inferred from sequence orthology PubMed 23382219. Source: MGI

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 19234461. Source: UniProtKB

receptor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323
Chain24 – 771748Integrin beta-2
PRO_0000016342

Regions

Topological domain24 – 702679Extracellular Potential
Transmembrane703 – 72523Helical; Potential
Topological domain726 – 77146Cytoplasmic Potential
Domain125 – 364240VWFA
Repeat450 – 49748I
Repeat498 – 54144II
Repeat542 – 58241III
Repeat583 – 61836IV
Region450 – 618169Cysteine-rich tandem repeats

Sites

Metal binding1391Calcium; via carbonyl oxygen By similarity
Metal binding1421Calcium By similarity
Metal binding1431Calcium By similarity
Metal binding3481Calcium By similarity

Amino acid modifications

Modified residue241Pyrrolidone carboxylic acid By similarity
Modified residue7471Phosphoserine By similarity
Modified residue7581Phosphoserine By similarity
Modified residue7601Phosphothreonine By similarity
Modified residue7621Phosphothreonine By similarity
Glycosylation511N-linked (GlcNAc...) Potential
Glycosylation1171N-linked (GlcNAc...) Potential
Glycosylation2551N-linked (GlcNAc...) Potential
Glycosylation5021N-linked (GlcNAc...) Potential
Glycosylation6261N-linked (GlcNAc...) Potential
Glycosylation6441N-linked (GlcNAc...) Potential
Disulfide bond26 ↔ 44 By similarity
Disulfide bond34 ↔ 448 By similarity
Disulfide bond37 ↔ 63 By similarity
Disulfide bond47 ↔ 74 By similarity
Disulfide bond192 ↔ 199 By similarity
Disulfide bond247 ↔ 287 By similarity
Disulfide bond387 ↔ 401 By similarity
Disulfide bond421 ↔ 446 By similarity
Disulfide bond450 ↔ 468 By similarity
Disulfide bond460 ↔ 471 By similarity
Disulfide bond473 ↔ 482 By similarity
Disulfide bond484 ↔ 515 By similarity
Disulfide bond498 ↔ 513 By similarity
Disulfide bond507 ↔ 518 By similarity
Disulfide bond520 ↔ 535 By similarity
Disulfide bond537 ↔ 560 By similarity
Disulfide bond542 ↔ 558 By similarity
Disulfide bond550 ↔ 563 By similarity
Disulfide bond565 ↔ 574 By similarity
Disulfide bond576 ↔ 599 By similarity
Disulfide bond583 ↔ 597 By similarity
Disulfide bond591 ↔ 602 By similarity
Disulfide bond604 ↔ 613 By similarity
Disulfide bond616 ↔ 619 By similarity
Disulfide bond623 ↔ 664 By similarity
Disulfide bond630 ↔ 649 By similarity
Disulfide bond633 ↔ 645 By similarity
Disulfide bond672 ↔ 697 By similarity

Experimental info

Sequence conflict4 – 63LRP → PH in CAA33077. Ref.1
Sequence conflict4491Q → H in AAA39325. Ref.2
Sequence conflict6671R → K in AAA39325. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P11835 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: 6FA51DA93AB40D8A

FASTA77185,026
        10         20         30         40         50         60 
MLGLRPSLLL ALAGLFFLGS AVSQECTKYK VSSCRDCIQS GPGCSWCQKL NFTGPGEPDS 

        70         80         90        100        110        120 
LRCDTRAQLL LKGCPADDIM DPRSIANPEF DQRGQRKQLS PQKVTLYLRP GQAAAFNVTF 

       130        140        150        160        170        180 
RRAKGYPIDL YYLMDLSYSM LDDLNNVKKL GGDLLQALNE ITESGRIGFG SFVDKTVLPF 

       190        200        210        220        230        240 
VNTHPEKLRN PCPNKEKACQ PPFAFRHVLK LTDNSNQFQT EVGKQLISGN LDAPEGGLDA 

       250        260        270        280        290        300 
IMQVAACPEE IGWRNVTRLL VFATDDGFHF AGDGKLGAIL TPNDGRCHLE DNMYKRSNEF 

       310        320        330        340        350        360 
DYPSVGQLAH KLSESNIQPI FAVTKKMVKT YEKLTEIIPK SAVGELSDDS SNVVQLIKNA 

       370        380        390        400        410        420 
YYKLSSRVFL DHSTLPDTLK VTYDSFCSNG ASSIGKSRGD CDGVQINNPV TFQVKVMASE 

       430        440        450        460        470        480 
CIQEQSFVIR ALGFTDTVTV QVRPQCECQC RDQSREQSLC GGKGVMECGI CRCESGYIGK 

       490        500        510        520        530        540 
NCECQTQGRS SQELERNCRK DNSSIVCSGL GDCICGQCVC HTSDVPNKEI FGQYCECDNV 

       550        560        570        580        590        600 
NCERYNSQVC GGSDRGSCNC GKCSCKPGYE GSACQCQRST TGCLNARLVE CSGRGHCQCN 

       610        620        630        640        650        660 
RCICDEGYQP PMCEDCPSCG SHCRDNHTSC AECLKFDKGP FEKNCSVQCA GMTLQTIPLK 

       670        680        690        700        710        720 
KKPCKERDSE GCWITYTLQQ KDGRNIYNIH VEDSLECVKG PNVAAIVGGT VVGVVLIGVL 

       730        740        750        760        770 
LLVIWKALTH LTDLREYRRF EKEKLKSQWN NDNPLFKSAT TTVMNPKFAE S 

« Hide

References

[1]"Nucleotide sequence of the cDNA from the mouse leukocyte adhesion protein CD18."
Wilson R.W., O'Brien W.E., Beaudet A.L.
Nucleic Acids Res. 17:5397-5397(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6 X CBA.
Tissue: Thymus.
[2]"Mouse macrophage beta subunit (CD11b) cDNA for the CR3 complement receptor/Mac-1 antigen."
Zeger D.L., Osman N., Hennings M., McKenzie I.F., Sears D.W., Hogarth P.M.
Immunogenetics 31:191-197(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Mouse macrophage beta subunit (CD11b) cDNA for the CR3 complement receptor/Mac-1 antigen."
Kofler R.
Immunogenetics 33:77-77(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO N-TERMINUS.
[4]"Nonmuscle myosin light-chain kinase mediates neutrophil transmigration in sepsis-induced lung inflammation by activating beta2 integrins."
Xu J., Gao X.-P., Ramchandran R., Zhao Y.-Y., Vogel S.M., Malik A.B.
Nat. Immunol. 9:880-886(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION DURING LUNG INJURY.
[5]"c-Abl and Src-family kinases cross-talk in regulation of myeloid cell migration."
Baruzzi A., Iacobucci I., Soverini S., Lowell C.A., Martinelli G., Berton G.
FEBS Lett. 584:15-21(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH FGR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14951 mRNA. Translation: CAA33077.1.
M31039 mRNA. Translation: AAA39325.1.
PIRA45839.
S04847.
RefSeqNP_032430.2. NM_008404.4.
UniGeneMm.1137.

3D structure databases

ProteinModelPortalP11835.
SMRP11835. Positions 24-698.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200828. 1 interaction.
DIPDIP-37428N.
IntActP11835. 6 interactions.
MINTMINT-1662243.

Chemistry

BindingDBP11835.

PTM databases

PhosphoSiteP11835.

Proteomic databases

MaxQBP11835.
PaxDbP11835.
PRIDEP11835.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID16414.
KEGGmmu:16414.

Organism-specific databases

CTD3689.
MGIMGI:96611. Itgb2.

Phylogenomic databases

eggNOGNOG287997.
HOGENOMHOG000252936.
HOVERGENHBG006190.
InParanoidP11835.
KOK06464.

Gene expression databases

CleanExMM_ITGB2.
GenevestigatorP11835.

Family and domain databases

Gene3D3.40.50.410. 1 hit.
InterProIPR015812. Integrin_bsu.
IPR015439. Integrin_bsu-2.
IPR014836. Integrin_bsu_cyt_dom.
IPR002369. Integrin_bsu_N.
IPR012896. Integrin_bsu_tail.
IPR016201. Plexin-like_fold.
IPR002035. VWF_A.
[Graphical view]
PANTHERPTHR10082. PTHR10082. 1 hit.
PTHR10082:SF15. PTHR10082:SF15. 1 hit.
PfamPF08725. Integrin_b_cyt. 1 hit.
PF07965. Integrin_B_tail. 1 hit.
PF00362. Integrin_beta. 1 hit.
[Graphical view]
PIRSFPIRSF002512. Integrin_B. 1 hit.
PRINTSPR01186. INTEGRINB.
SMARTSM00187. INB. 1 hit.
SM00423. PSI. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMSSF103575. SSF103575. 1 hit.
SSF53300. SSF53300. 1 hit.
SSF69687. SSF69687. 1 hit.
PROSITEPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 3 hits.
PS00243. INTEGRIN_BETA. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio289605.
PROP11835.
SOURCESearch...

Entry information

Entry nameITB2_MOUSE
AccessionPrimary (citable) accession number: P11835
Secondary accession number(s): Q64482
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 15, 1998
Last modified: July 9, 2014
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot