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Protein

Nitrate reductase [NADH] 1

Gene

NIA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NAD+ + H2O = nitrate + NADH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD per subunit.By similarity
  • hemeBy similarityNote: Binds 1 heme group per subunit.By similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi197MolybdenumBy similarity1
Metal bindingi580Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi603Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • nitrate assimilation Source: TAIR
  • nitric oxide biosynthetic process Source: TAIR
  • response to herbicide Source: UniProtKB-KW
  • response to light stimulus Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Herbicide resistance, Nitrate assimilation

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD

Enzyme and pathway databases

BioCyciMetaCyc:AT1G77760-MONOMER.
ReactomeiR-ATH-114608. Platelet degranulation.
R-ATH-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-ATH-196836. Vitamin C (ascorbate) metabolism.
R-ATH-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase [NADH] 1 (EC:1.7.1.1)
Short name:
NR1
Gene namesi
Name:NIA1
Ordered Locus Names:At1g77760
ORF Names:T32E8.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G77760.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi198A → T: Loss of activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660491 – 917Nitrate reductase [NADH] 1Add BLAST917

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi436InterchainSequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP11832.
PRIDEiP11832.

PTM databases

iPTMnetiP11832.

Expressioni

Tissue specificityi

Root, leaf, and shoot.

Gene expression databases

GenevisibleiP11832. AT.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi29331. 8 interactors.
IntActiP11832. 4 interactors.
STRINGi3702.AT1G77760.1.

Structurei

3D structure databases

ProteinModelPortaliP11832.
SMRiP11832.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini545 – 620Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST76
Domaini660 – 772FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the nitrate reductase family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410JWE3. Eukaryota.
KOG0534. Eukaryota.
KOG0535. Eukaryota.
KOG0537. Eukaryota.
COG0543. LUCA.
COG2041. LUCA.
COG5274. LUCA.
HOGENOMiHOG000252609.
InParanoidiP11832.
KOiK10534.
OMAiSPFMNTA.
OrthoDBiEOG0936034C.
PhylomeDBiP11832.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11832-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSVDNRHY PTMNGVAHAF KPPLVPSPRS FDRHRHQNQT LDVILTETKI
60 70 80 90 100
VKETEVITTV VDSYDDSSSD DEDESHNRNV PYYKELVKKS NSDLEPSILD
110 120 130 140 150
PRDESTADSW IQRNSSMLRL TGKHPFNAEA PLPRLMHHGF ITPVPLHYVR
160 170 180 190 200
NHGAVPKANW SDWSIEITGL VKRPAKFTME ELISEFPSRE FPVTLVCAGN
210 220 230 240 250
RRKEQNMVKQ TIGFNWGSAG VSTSLWKGIP LSEILRRCGI YSRRGGALNV
260 270 280 290 300
CFEGAEDLPG GGGSKYGTSI KKEMAMDPAR DIILAYMQNG ELLTPDHGFP
310 320 330 340 350
VRVIVPGFIG GRMVKWLKRI IVTPQESDSY YHYKDNRVLP SLVDAELANS
360 370 380 390 400
EAWWYKPEYI INELNINSVI TTPGHAEILP INAFTTQKPY TLKGYAYSGG
410 420 430 440 450
GKKVTRVEVT LDGGDTWSVC ELDHQEKPNK YGKFWCWCFW SLDVEVLDLL
460 470 480 490 500
SAKDVAVRAW DESFNTQPDK LIWNLMGMMN NCWFRIRTNV CKPHRGEIGI
510 520 530 540 550
VFEHPTRPGN QSGGWMAKER QLEISSESNN TLKKSVSSPF MNTASKMYSI
560 570 580 590 600
SEVRKHNTAD SAWIIVHGHI YDCTRFLKDH PGGTDSILIN AGTDCTEEFE
610 620 630 640 650
AIHSDKAKKL LEDYRIGELI TTGYDSSPNV SVHGASNFGP LLAPIKELTP
660 670 680 690 700
QKNIALVNPR EKIPVRLIEK TSISHDVRKF RFALPSEDQQ LGLPVGKHVF
710 720 730 740 750
VCANINDKLC LRAYTPTSAI DAVGHIDLVV KVYFKDVHPR FPNGGLMSQH
760 770 780 790 800
LDSLPIGSMI DIKGPLGHIE YKGKGNFLVS GKPKFAKKLA MLAGGTGITP
810 820 830 840 850
IYQIIQSILS DPEDETEMYV VYANRTEDDI LVREELEGWA SKHKERLKIW
860 870 880 890 900
YVVEIAKEGW SYSTGFITEA VLREHIPEGL EGESLALACG PPPMIQFALQ
910
PNLEKMGYNV KEDLLIF
Length:917
Mass (Da):103,041
Last modified:June 6, 2002 - v3
Checksum:i6FD3ED46B9F63825
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17A → R in CAA79494 (PubMed:8510658).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z19050 Genomic DNA. Translation: CAA79494.1.
AC012193 Genomic DNA. Translation: AAG51627.1.
CP002684 Genomic DNA. Translation: AEE36018.1.
AY090950 mRNA. Translation: AAM13997.1.
AF424624 mRNA. Translation: AAL11617.1.
BT000989 mRNA. Translation: AAN41389.1.
X13434 mRNA. Translation: CAA31786.1.
X13436 Genomic DNA. Translation: CAA31788.1.
PIRiE96807.
S35228.
RefSeqiNP_177899.1. NM_106425.3.
UniGeneiAt.17771.

Genome annotation databases

EnsemblPlantsiAT1G77760.1; AT1G77760.1; AT1G77760.
GeneIDi844112.
GrameneiAT1G77760.1; AT1G77760.1; AT1G77760.
KEGGiath:AT1G77760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z19050 Genomic DNA. Translation: CAA79494.1.
AC012193 Genomic DNA. Translation: AAG51627.1.
CP002684 Genomic DNA. Translation: AEE36018.1.
AY090950 mRNA. Translation: AAM13997.1.
AF424624 mRNA. Translation: AAL11617.1.
BT000989 mRNA. Translation: AAN41389.1.
X13434 mRNA. Translation: CAA31786.1.
X13436 Genomic DNA. Translation: CAA31788.1.
PIRiE96807.
S35228.
RefSeqiNP_177899.1. NM_106425.3.
UniGeneiAt.17771.

3D structure databases

ProteinModelPortaliP11832.
SMRiP11832.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi29331. 8 interactors.
IntActiP11832. 4 interactors.
STRINGi3702.AT1G77760.1.

PTM databases

iPTMnetiP11832.

Proteomic databases

PaxDbiP11832.
PRIDEiP11832.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G77760.1; AT1G77760.1; AT1G77760.
GeneIDi844112.
GrameneiAT1G77760.1; AT1G77760.1; AT1G77760.
KEGGiath:AT1G77760.

Organism-specific databases

TAIRiAT1G77760.

Phylogenomic databases

eggNOGiENOG410JWE3. Eukaryota.
KOG0534. Eukaryota.
KOG0535. Eukaryota.
KOG0537. Eukaryota.
COG0543. LUCA.
COG2041. LUCA.
COG5274. LUCA.
HOGENOMiHOG000252609.
InParanoidiP11832.
KOiK10534.
OMAiSPFMNTA.
OrthoDBiEOG0936034C.
PhylomeDBiP11832.

Enzyme and pathway databases

BioCyciMetaCyc:AT1G77760-MONOMER.
ReactomeiR-ATH-114608. Platelet degranulation.
R-ATH-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-ATH-196836. Vitamin C (ascorbate) metabolism.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiP11832.

Gene expression databases

GenevisibleiP11832. AT.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIA1_ARATH
AccessioniPrimary (citable) accession number: P11832
Secondary accession number(s): Q9CA18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: June 6, 2002
Last modified: November 30, 2016
This is version 160 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

When mutated confers resistance to the herbicide chlorate.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.