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Protein

Nitrate reductase [NADH] 1

Gene

NIA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Miscellaneous

When mutated confers resistance to the herbicide chlorate.

Catalytic activityi

Nitrite + NAD+ + H2O = nitrate + NADH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD per subunit.By similarity
  • hemeBy similarityNote: Binds 1 heme group per subunit.By similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi197MolybdenumBy similarity1
Metal bindingi580Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi603Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • nitrate assimilation Source: TAIR
  • nitric oxide biosynthetic process Source: TAIR
  • response to herbicide Source: UniProtKB-KW
  • response to light stimulus Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processHerbicide resistance, Nitrate assimilation
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD

Enzyme and pathway databases

BioCyciMetaCyc:AT1G77760-MONOMER
ReactomeiR-ATH-114608 Platelet degranulation
R-ATH-196836 Vitamin C (ascorbate) metabolism
R-ATH-211945 Phase I - Functionalization of compounds
R-ATH-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase [NADH] 1 (EC:1.7.1.1)
Short name:
NR1
Gene namesi
Name:NIA1
Ordered Locus Names:At1g77760
ORF Names:T32E8.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G77760
TAIRilocus:2203221 AT1G77760

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi198A → T: Loss of activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660491 – 917Nitrate reductase [NADH] 1Add BLAST917

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi436InterchainSequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP11832
PRIDEiP11832

PTM databases

iPTMnetiP11832

Expressioni

Tissue specificityi

Root, leaf, and shoot.

Gene expression databases

ExpressionAtlasiP11832 baseline and differential
GenevisibleiP11832 AT

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi29331, 8 interactors
IntActiP11832, 4 interactors
STRINGi3702.AT1G77760.1

Structurei

3D structure databases

ProteinModelPortaliP11832
SMRiP11832
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini545 – 620Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST76
Domaini660 – 772FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the nitrate reductase family.Curated

Phylogenomic databases

eggNOGiENOG410JWE3 Eukaryota
KOG0534 Eukaryota
KOG0535 Eukaryota
KOG0537 Eukaryota
COG0543 LUCA
COG2041 LUCA
COG5274 LUCA
HOGENOMiHOG000252609
InParanoidiP11832
KOiK10534
OMAiQPRDMYW
OrthoDBiEOG0936034C
PhylomeDBiP11832

Family and domain databases

Gene3Di3.10.120.10, 1 hit
3.90.420.10, 1 hit
InterProiView protein in InterPro
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
IPR017927 Fd_Rdtase_FAD-bd
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR014756 Ig_E-set
IPR005066 MoCF_OxRdtse_dimer
IPR008335 Mopterin_OxRdtase_euk
IPR001834 NADH-Cyt_B5_reductase
IPR012137 Nitr_rd_NADH
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR000572 OxRdtase_Mopterin-bd_dom
IPR036374 OxRdtase_Mopterin-bd_sf
IPR022407 OxRdtase_Mopterin_BS
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PF00970 FAD_binding_6, 1 hit
PF03404 Mo-co_dimer, 1 hit
PF00175 NAD_binding_1, 1 hit
PF00174 Oxidored_molyb, 1 hit
PIRSFiPIRSF000233 Nitr_rd_NADH, 1 hit
PRINTSiPR00406 CYTB5RDTASE
PR00363 CYTOCHROMEB5
PR00407 EUMOPTERIN
PR00371 FPNCR
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF55856 SSF55856, 1 hit
SSF56524 SSF56524, 1 hit
SSF63380 SSF63380, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit
PS51384 FAD_FR, 1 hit
PS00559 MOLYBDOPTERIN_EUK, 1 hit

Sequencei

Sequence statusi: Complete.

P11832-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSVDNRHY PTMNGVAHAF KPPLVPSPRS FDRHRHQNQT LDVILTETKI
60 70 80 90 100
VKETEVITTV VDSYDDSSSD DEDESHNRNV PYYKELVKKS NSDLEPSILD
110 120 130 140 150
PRDESTADSW IQRNSSMLRL TGKHPFNAEA PLPRLMHHGF ITPVPLHYVR
160 170 180 190 200
NHGAVPKANW SDWSIEITGL VKRPAKFTME ELISEFPSRE FPVTLVCAGN
210 220 230 240 250
RRKEQNMVKQ TIGFNWGSAG VSTSLWKGIP LSEILRRCGI YSRRGGALNV
260 270 280 290 300
CFEGAEDLPG GGGSKYGTSI KKEMAMDPAR DIILAYMQNG ELLTPDHGFP
310 320 330 340 350
VRVIVPGFIG GRMVKWLKRI IVTPQESDSY YHYKDNRVLP SLVDAELANS
360 370 380 390 400
EAWWYKPEYI INELNINSVI TTPGHAEILP INAFTTQKPY TLKGYAYSGG
410 420 430 440 450
GKKVTRVEVT LDGGDTWSVC ELDHQEKPNK YGKFWCWCFW SLDVEVLDLL
460 470 480 490 500
SAKDVAVRAW DESFNTQPDK LIWNLMGMMN NCWFRIRTNV CKPHRGEIGI
510 520 530 540 550
VFEHPTRPGN QSGGWMAKER QLEISSESNN TLKKSVSSPF MNTASKMYSI
560 570 580 590 600
SEVRKHNTAD SAWIIVHGHI YDCTRFLKDH PGGTDSILIN AGTDCTEEFE
610 620 630 640 650
AIHSDKAKKL LEDYRIGELI TTGYDSSPNV SVHGASNFGP LLAPIKELTP
660 670 680 690 700
QKNIALVNPR EKIPVRLIEK TSISHDVRKF RFALPSEDQQ LGLPVGKHVF
710 720 730 740 750
VCANINDKLC LRAYTPTSAI DAVGHIDLVV KVYFKDVHPR FPNGGLMSQH
760 770 780 790 800
LDSLPIGSMI DIKGPLGHIE YKGKGNFLVS GKPKFAKKLA MLAGGTGITP
810 820 830 840 850
IYQIIQSILS DPEDETEMYV VYANRTEDDI LVREELEGWA SKHKERLKIW
860 870 880 890 900
YVVEIAKEGW SYSTGFITEA VLREHIPEGL EGESLALACG PPPMIQFALQ
910
PNLEKMGYNV KEDLLIF
Length:917
Mass (Da):103,041
Last modified:June 6, 2002 - v3
Checksum:i6FD3ED46B9F63825
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17A → R in CAA79494 (PubMed:8510658).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z19050 Genomic DNA Translation: CAA79494.1
AC012193 Genomic DNA Translation: AAG51627.1
CP002684 Genomic DNA Translation: AEE36018.1
AY090950 mRNA Translation: AAM13997.1
AF424624 mRNA Translation: AAL11617.1
BT000989 mRNA Translation: AAN41389.1
X13434 mRNA Translation: CAA31786.1
X13436 Genomic DNA Translation: CAA31788.1
PIRiE96807
S35228
RefSeqiNP_177899.1, NM_106425.3
UniGeneiAt.17771

Genome annotation databases

EnsemblPlantsiAT1G77760.1; AT1G77760.1; AT1G77760
GeneIDi844112
GrameneiAT1G77760.1; AT1G77760.1; AT1G77760
KEGGiath:AT1G77760

Similar proteinsi

Entry informationi

Entry nameiNIA1_ARATH
AccessioniPrimary (citable) accession number: P11832
Secondary accession number(s): Q9CA18
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: June 6, 2002
Last modified: May 23, 2018
This is version 169 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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