Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serum response factor

Gene

SRF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi133 – 222Add BLAST90

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000112658-MONOMER.
ReactomeiR-HSA-5663220. RHO GTPases Activate Formins.
SignaLinkiP11831.
SIGNORiP11831.

Names & Taxonomyi

Protein namesi
Recommended name:
Serum response factor
Short name:
SRF
Gene namesi
Name:SRF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:11291. SRF.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • nuclear chromatin Source: Ensembl
  • nucleoplasm Source: Reactome
  • nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6722.
OpenTargetsiENSG00000112658.
PharmGKBiPA36116.

Polymorphism and mutation databases

BioMutaiSRF.
DMDMi134876.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001994231 – 508Serum response factorAdd BLAST508

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei77PhosphoserineCombined sources1 Publication1
Modified residuei79PhosphoserineCombined sources1 Publication1
Modified residuei83PhosphoserineCombined sources1 Publication1
Modified residuei85PhosphoserineCombined sources1 Publication1
Modified residuei103Phosphoserine1 Publication1
Modified residuei224PhosphoserineCombined sources1
Modified residuei253Phosphoserine1 Publication1
GlycosylationiCAR_000181277O-linked (GlcNAc)1 Publication1
Glycosylationi307O-linked (GlcNAc)1 Publication1
Glycosylationi309O-linked (GlcNAc)1 Publication1
GlycosylationiCAR_000196316O-linked (GlcNAc)1 Publication1
Glycosylationi383O-linked (GlcNAc)1 Publication1
Modified residuei435Phosphoserine; by dsDNA kinase1 Publication1
Modified residuei446Phosphoserine; by dsDNA kinase1 Publication1

Post-translational modificationi

Phosphorylated by PRKDC.3 Publications

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP11831.
MaxQBiP11831.
PaxDbiP11831.
PeptideAtlasiP11831.
PRIDEiP11831.

PTM databases

iPTMnetiP11831.
PhosphoSitePlusiP11831.
UniCarbKBiP11831.

Miscellaneous databases

PMAP-CutDBP11831.

Expressioni

Gene expression databases

BgeeiENSG00000112658.
CleanExiHS_SRF.
ExpressionAtlasiP11831. baseline and differential.
GenevisibleiP11831. HS.

Organism-specific databases

HPAiCAB005416.
HPA001819.

Interactioni

Subunit structurei

Binds DNA as a multimer, probably a dimer. Interacts with MLLT7/FOXO4, NKX3A and SSRP1. Interacts with ARID2 and SRFBP1 (By similarity). Forms complexes with ARID2, MYOCD, NKX2-5 and SRFBP1 (By similarity). Forms a nuclear ternary complex with MKL1 and SCAI (PubMed:19350017). Interacts with LPXN (PubMed:18497331). Interacts with OLFM2; the interaction promotes dissociation of SRF from the transcriptional repressor HEY2, facilitates binding of SRF to target genes and promotes smooth muscle differentiation (PubMed:25298399).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MKL1Q969V62EBI-493034,EBI-493122
MKL2Q9ULH73EBI-493034,EBI-493007
MYOCDQ8IZQ82EBI-493034,EBI-493384

GO - Molecular functioni

  • protein homodimerization activity Source: BHF-UCL
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi112600. 61 interactors.
DIPiDIP-49N.
IntActiP11831. 6 interactors.
MINTiMINT-130058.
STRINGi9606.ENSP00000265354.

Structurei

Secondary structure

1508
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi154 – 179Combined sources26
Beta strandi182 – 188Combined sources7
Beta strandi190 – 192Combined sources3
Beta strandi194 – 198Combined sources5
Helixi200 – 205Combined sources6
Helixi209 – 220Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HBXX-ray3.15A/B/D/E132-223[»]
1K6OX-ray3.19B/C133-235[»]
1SRSX-ray3.20A/B132-223[»]
DisProtiDP00574.
ProteinModelPortaliP11831.
SMRiP11831.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11831.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini141 – 201MADS-boxPROSITE-ProRule annotationAdd BLAST61

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni168 – 222Involved in dimerizationAdd BLAST55

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi13 – 142Gly-richAdd BLAST130
Compositional biasi80 – 90Asp/Glu-rich (acidic)Add BLAST11
Compositional biasi242 – 258Asp/Glu-rich (acidic)Add BLAST17

Sequence similaritiesi

Contains 1 MADS-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0015. Eukaryota.
COG5068. LUCA.
GeneTreeiENSGT00400000022158.
HOGENOMiHOG000012380.
HOVERGENiHBG014968.
InParanoidiP11831.
KOiK04378.
OMAiNAFPQAP.
OrthoDBiEOG091G0X24.
PhylomeDBiP11831.
TreeFamiTF318482.

Family and domain databases

CDDicd00266. MADS_SRF_like. 1 hit.
InterProiIPR033897. MADS_SRF-like.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11831-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPTQAGAAA ALGRGSALGG SLNRTPTGRP GGGGGTRGAN GGRVPGNGAG
60 70 80 90 100
LGPGRLEREA AAAAATTPAP TAGALYSGSE GDSESGEEEE LGAERRGLKR
110 120 130 140 150
SLSEMEIGMV VGGPEASAAA TGGYGPVSGA VSGAKPGKKT RGRVKIKMEF
160 170 180 190 200
IDNKLRRYTT FSKRKTGIMK KAYELSTLTG TQVLLLVASE TGHVYTFATR
210 220 230 240 250
KLQPMITSET GKALIQTCLN SPDSPPRSDP TTDQRMSATG FEETDLTYQV
260 270 280 290 300
SESDSSGETK DTLKPAFTVT NLPGTTSTIQ TAPSTSTTMQ VSSGPSFPIT
310 320 330 340 350
NYLAPVSASV SPSAVSSANG TVLKSTGSGP VSSGGLMQLP TSFTLMPGGA
360 370 380 390 400
VAQQVPVQAI QVHQAPQQAS PSRDSSTDLT QTSSSGTVTL PATIMTSSVP
410 420 430 440 450
TTVGGHMMYP SPHAVMYAPT SGLGDGSLTV LNAFSQAPST MQVSHSQVQE
460 470 480 490 500
PGGVPQVFLT ASSGTVQIPV SAVQLHQMAV IGQQAGSSSN LTELQVVNLD

TAHSTKSE
Length:508
Mass (Da):51,593
Last modified:October 1, 1989 - v1
Checksum:i25505828D3276F44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03161 mRNA. Translation: AAA36647.1.
AL133375, AL355385 Genomic DNA. Translation: CAI20207.1.
AL355385, AL133375 Genomic DNA. Translation: CAI13785.1.
CH471081 Genomic DNA. Translation: EAX04161.1.
BC048211 mRNA. Translation: AAH48211.1.
BC052572 mRNA. Translation: AAH52572.1.
CCDSiCCDS4889.1.
PIRiA31637.
RefSeqiNP_001278930.1. NM_001292001.1.
NP_003122.1. NM_003131.3.
UniGeneiHs.520140.

Genome annotation databases

EnsembliENST00000265354; ENSP00000265354; ENSG00000112658.
GeneIDi6722.
KEGGihsa:6722.
UCSCiuc003oui.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03161 mRNA. Translation: AAA36647.1.
AL133375, AL355385 Genomic DNA. Translation: CAI20207.1.
AL355385, AL133375 Genomic DNA. Translation: CAI13785.1.
CH471081 Genomic DNA. Translation: EAX04161.1.
BC048211 mRNA. Translation: AAH48211.1.
BC052572 mRNA. Translation: AAH52572.1.
CCDSiCCDS4889.1.
PIRiA31637.
RefSeqiNP_001278930.1. NM_001292001.1.
NP_003122.1. NM_003131.3.
UniGeneiHs.520140.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HBXX-ray3.15A/B/D/E132-223[»]
1K6OX-ray3.19B/C133-235[»]
1SRSX-ray3.20A/B132-223[»]
DisProtiDP00574.
ProteinModelPortaliP11831.
SMRiP11831.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112600. 61 interactors.
DIPiDIP-49N.
IntActiP11831. 6 interactors.
MINTiMINT-130058.
STRINGi9606.ENSP00000265354.

PTM databases

iPTMnetiP11831.
PhosphoSitePlusiP11831.
UniCarbKBiP11831.

Polymorphism and mutation databases

BioMutaiSRF.
DMDMi134876.

Proteomic databases

EPDiP11831.
MaxQBiP11831.
PaxDbiP11831.
PeptideAtlasiP11831.
PRIDEiP11831.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265354; ENSP00000265354; ENSG00000112658.
GeneIDi6722.
KEGGihsa:6722.
UCSCiuc003oui.4. human.

Organism-specific databases

CTDi6722.
DisGeNETi6722.
GeneCardsiSRF.
HGNCiHGNC:11291. SRF.
HPAiCAB005416.
HPA001819.
MIMi600589. gene.
neXtProtiNX_P11831.
OpenTargetsiENSG00000112658.
PharmGKBiPA36116.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0015. Eukaryota.
COG5068. LUCA.
GeneTreeiENSGT00400000022158.
HOGENOMiHOG000012380.
HOVERGENiHBG014968.
InParanoidiP11831.
KOiK04378.
OMAiNAFPQAP.
OrthoDBiEOG091G0X24.
PhylomeDBiP11831.
TreeFamiTF318482.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000112658-MONOMER.
ReactomeiR-HSA-5663220. RHO GTPases Activate Formins.
SignaLinkiP11831.
SIGNORiP11831.

Miscellaneous databases

EvolutionaryTraceiP11831.
GeneWikiiSerum_response_factor.
GenomeRNAii6722.
PMAP-CutDBP11831.
PROiP11831.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000112658.
CleanExiHS_SRF.
ExpressionAtlasiP11831. baseline and differential.
GenevisibleiP11831. HS.

Family and domain databases

CDDicd00266. MADS_SRF_like. 1 hit.
InterProiIPR033897. MADS_SRF-like.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRF_HUMAN
AccessioniPrimary (citable) accession number: P11831
Secondary accession number(s): Q5T648
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 30, 2016
This is version 190 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.