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P11818

- SPIKE_ADE05

UniProt

P11818 - SPIKE_ADE05

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Protein

Fiber protein

Gene

L5

Organism
Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Forms spikes that protrude from each vertex of the icosahedral capsid. Interacts with host coxsackievirus and adenovirus receptor CXADR located at the cell tight junctions to provide virion initial attachment to target cell. The fiber protein binds to CXADR with a higher affinity than CXADR binds to itself, thereby blocking the cell-cell adhesion function of CXADR dimers and leading to local disruption of the tight junction. Fiber protein present on neo-synthesized particles may thus disrupt the junctional integrity in order to facilitate further neighboring cells infection. Fiber proteins are shed during virus entry, when virus is still at the cell surface. Fiber shedding is dependent on viral CXADR drifting motion and subsequent binding to immobile integrins. Heparan sulfate might also play a role in virus binding (By similarity).By similarity

GO - Biological processi

  1. cell adhesion Source: InterPro
  2. pathogenesis Source: InterPro
  3. viral entry into host cell Source: UniProtKB-KW
  4. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral attachment to host adhesion receptor, Viral attachment to host cell, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Fiber protein
Short name:
SPIKE
Alternative name(s):
Protein IV
Gene namesi
ORF Names:L5
OrganismiHuman adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Taxonomic identifieri28285 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000004992: Genome

Subcellular locationi

Virion By similarity. Host nucleus By similarity
Note: Anchored to the pentons, protrudes from the virion surface. Present in 36 copies per virion (By similarity).By similarity

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-KW
  2. viral capsid, fiber Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 581581Fiber proteinPRO_0000221789Add
BLAST

Post-translational modificationi

O-glycosylated; glycans contain N-acetylglucosamine and may play a role in fiber assembly and stabilization.By similarity

PTM databases

UniCarbKBiP11818.

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homotrimer; arranged in a triple beta-spiral. Interacts with host receptor CXADR. Interacts (via N-terminal tail region) with pentons (By similarity).By similarity

Structurei

Secondary structure

1
581
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi397 – 3993
Beta strandi400 – 4034
Beta strandi413 – 4153
Beta strandi419 – 42810
Beta strandi431 – 44010
Helixi443 – 4453
Turni446 – 4483
Beta strandi453 – 4619
Beta strandi470 – 4734
Helixi475 – 4773
Beta strandi479 – 4824
Helixi485 – 4873
Helixi495 – 4973
Turni501 – 5033
Helixi508 – 5103
Helixi512 – 5143
Beta strandi515 – 5228
Beta strandi528 – 53710
Beta strandi543 – 5453
Beta strandi549 – 5579
Beta strandi573 – 5797

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KNBX-ray1.70A386-581[»]
4ATZX-ray1.95A/B/C387-581[»]
ProteinModelPortaliP11818.
SMRiP11818. Positions 321-581.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11818.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati45 – 5915Shaft 1Add
BLAST
Repeati60 – 7516Shaft 2Add
BLAST
Repeati76 – 9520Shaft 3Add
BLAST
Repeati96 – 10914Shaft 4Add
BLAST
Repeati110 – 12415Shaft 5Add
BLAST
Repeati125 – 13915Shaft 6Add
BLAST
Repeati140 – 15415Shaft 7Add
BLAST
Repeati155 – 17016Shaft 8Add
BLAST
Repeati171 – 18515Shaft 9Add
BLAST
Repeati186 – 20015Shaft 10Add
BLAST
Repeati201 – 21717Shaft 11Add
BLAST
Repeati218 – 23215Shaft 12Add
BLAST
Repeati233 – 24816Shaft 13Add
BLAST
Repeati249 – 26517Shaft 14Add
BLAST
Repeati266 – 27914Shaft 15Add
BLAST
Repeati280 – 29617Shaft 16Add
BLAST
Repeati297 – 31620Shaft 17Add
BLAST
Repeati317 – 33317Shaft 18Add
BLAST
Repeati334 – 35421Shaft 19Add
BLAST
Repeati355 – 37016Shaft 20Add
BLAST
Repeati371 – 38616Shaft 21Add
BLAST
Repeati387 – 3926Shaft 22

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 4444Tail (penton base attachment)By similarityAdd
BLAST
Regioni45 – 392348Shaft regionBy similarityAdd
BLAST
Regioni393 – 3986SpacerBy similarity
Regioni399 – 581183HeadBy similarityAdd
BLAST

Domaini

The tail region anchors the fiber to penton base capsomers, whereas the shaft, built from several repeated motifs, allows the knob to protrude from the virion.By similarity

Sequence similaritiesi

Belongs to the adenoviridae fiber family.Curated
Contains 22 Shaft repeats.Curated

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di2.10.25.20. 3 hits.
2.60.90.10. 1 hit.
InterProiIPR000931. Adeno_fibre.
IPR000978. Adeno_fibre_knob.
IPR000939. Adenobir_fibre_prot_rpt/shaft.
IPR008982. Adenovirus_pIV-rel_att.
IPR009013. Attachment_protein_shaft_dom.
[Graphical view]
PfamiPF00541. Adeno_knob. 1 hit.
PF00608. Adeno_shaft. 6 hits.
[Graphical view]
PRINTSiPR00307. ADENOVSFIBRE.
SUPFAMiSSF49835. SSF49835. 1 hit.
SSF51225. SSF51225. 4 hits.

Sequencei

Sequence statusi: Complete.

P11818-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRARPSEDT FNPVYPYDTE TGPPTVPFLT PPFVSPNGFQ ESPPGVLSLR
60 70 80 90 100
LSEPLVTSNG MLALKMGNGL SLDEAGNLTS QNVTTVSPPL KKTKSNINLE
110 120 130 140 150
ISAPLTVTSE ALTVAAAAPL MVAGNTLTMQ SQAPLTVHDS KLSIATQGPL
160 170 180 190 200
TVSEGKLALQ TSGPLTTTDS STLTITASPP LTTATGSLGI DLKEPIYTQN
210 220 230 240 250
GKLGLKYGAP LHVTDDLNTL TVATGPGVTI NNTSLQTKVT GALGFDSQGN
260 270 280 290 300
MQLNVAGGLR IDSQNRRLIL DVSYPFDAQN QLNLRLGQGP LFINSAHNLD
310 320 330 340 350
INYNKGLYLF TASNNSKKLE VNLSTAKGLM FDATAIAINA GDGLEFGSPN
360 370 380 390 400
APNTNPLKTK IGHGLEFDSN KAMVPKLGTG LSFDSTGAIT VGNKNNDKLT
410 420 430 440 450
LWTTPAPSPN CRLNAEKDAK LTLVLTKCGS QILATVSVLA VKGSLAPISG
460 470 480 490 500
TVQSAHLIIR FDENGVLLNN SFLDPEYWNF RNGDLTEGTA YTNAVGFMPN
510 520 530 540 550
LSAYPKSHGK TAKSNIVSQV YLNGDKTKPV TLTITLNGTQ ETGDTTPSAY
560 570 580
SMSFSWDWSG HNYINEIFAT SSYTFSYIAQ E
Length:581
Mass (Da):61,585
Last modified:October 1, 1989 - v1
Checksum:iC3FDDFCF9E5E599F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M73260 Genomic DNA. No translation available.
M18369 Genomic DNA. Translation: AAA42504.1.
PIRiA27404. ERADF5.
RefSeqiAP_000226.1. AC_000008.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M73260 Genomic DNA. No translation available.
M18369 Genomic DNA. Translation: AAA42504.1 .
PIRi A27404. ERADF5.
RefSeqi AP_000226.1. AC_000008.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1KNB X-ray 1.70 A 386-581 [» ]
4ATZ X-ray 1.95 A/B/C 387-581 [» ]
ProteinModelPortali P11818.
SMRi P11818. Positions 321-581.
ModBasei Search...
MobiDBi Search...

PTM databases

UniCarbKBi P11818.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P11818.

Family and domain databases

Gene3Di 2.10.25.20. 3 hits.
2.60.90.10. 1 hit.
InterProi IPR000931. Adeno_fibre.
IPR000978. Adeno_fibre_knob.
IPR000939. Adenobir_fibre_prot_rpt/shaft.
IPR008982. Adenovirus_pIV-rel_att.
IPR009013. Attachment_protein_shaft_dom.
[Graphical view ]
Pfami PF00541. Adeno_knob. 1 hit.
PF00608. Adeno_shaft. 6 hits.
[Graphical view ]
PRINTSi PR00307. ADENOVSFIBRE.
SUPFAMi SSF49835. SSF49835. 1 hit.
SSF51225. SSF51225. 4 hits.
ProtoNeti Search...

Publicationsi

  1. "The sequence of adenovirus fiber: similarities and differences between serotypes 2 and 5."
    Chroboczek J., Jacrot B.
    Virology 161:549-554(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The sequence of the genome of adenovirus type 5 and its comparison with the genome of adenovirus type 2."
    Chroboczek J., Bieber F., Jacrot B.
    Virology 186:280-285(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "De novo derivation of proteomes from transcriptomes for transcript and protein identification."
    Evans V.C., Barker G., Heesom K.J., Fan J., Bessant C., Matthews D.A.
    Nat. Methods 9:1207-1211(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Relative accessibility of N-acetylglucosamine in trimers of the adenovirus types 2 and 5 fiber proteins."
    Mullis K.G., Haltiwanger R.S., Hart G.W., Marchase R.B., Engler J.A.
    J. Virol. 64:5317-5323(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION.
  5. Cited for: REVIEW.
  6. "Crystal structure of the receptor-binding domain of adenovirus type 5 fiber protein at 1.7-A resolution."
    Xia D., Henry L.J., Gerard R.D., Deisenhofer J.
    Structure 2:1259-1270(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 386-581.

Entry informationi

Entry nameiSPIKE_ADE05
AccessioniPrimary (citable) accession number: P11818
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: October 29, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell (By similarity).By similarity

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3