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P11818

- SPIKE_ADE05

UniProt

P11818 - SPIKE_ADE05

Protein

Fiber protein

Gene

L5

Organism
Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Oct 1989)
      Previous versions | rss
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    Functioni

    Forms spikes that protrude from each vertex of the icosahedral capsid. Interacts with host coxsackievirus and adenovirus receptor CXADR located at the cell tight junctions to provide virion initial attachment to target cell. The fiber protein binds to CXADR with a higher affinity than CXADR binds to itself, thereby blocking the cell-cell adhesion function of CXADR dimers and leading to local disruption of the tight junction. Fiber protein present on neo-synthesized particles may thus disrupt the junctional integrity in order to facilitate further neighboring cells infection. Fiber proteins are shed during virus entry, when virus is still at the cell surface. Fiber shedding is dependent on viral CXADR drifting motion and subsequent binding to immobile integrins. Heparan sulfate might also play a role in virus binding By similarity.By similarity

    GO - Biological processi

    1. cell adhesion Source: InterPro
    2. pathogenesis Source: InterPro
    3. viral entry into host cell Source: UniProtKB-KW
    4. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Viral attachment to host adhesion receptor, Viral attachment to host cell, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fiber protein
    Short name:
    SPIKE
    Alternative name(s):
    Protein IV
    Gene namesi
    ORF Names:L5
    OrganismiHuman adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
    Taxonomic identifieri28285 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000004992: Genome

    Subcellular locationi

    Virion By similarity. Host nucleus By similarity
    Note: Anchored to the pentons, protrudes from the virion surface. Present in 36 copies per virion By similarity.By similarity

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell
    2. viral capsid, fiber Source: CACAO

    Keywords - Cellular componenti

    Capsid protein, Host nucleus, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 581581Fiber proteinPRO_0000221789Add
    BLAST

    Post-translational modificationi

    O-glycosylated; glycans contain N-acetylglucosamine and may play a role in fiber assembly and stabilization.By similarity

    PTM databases

    UniCarbKBiP11818.

    Expressioni

    Inductioni

    Expressed in the late phase of the viral replicative cycle.

    Keywords - Developmental stagei

    Late protein

    Interactioni

    Subunit structurei

    Homotrimer; arranged in a triple beta-spiral. Interacts with host receptor CXADR. Interacts (via N-terminal tail region) with pentons By similarity.By similarity

    Structurei

    Secondary structure

    1
    581
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi397 – 3993
    Beta strandi400 – 4034
    Beta strandi413 – 4153
    Beta strandi419 – 42810
    Beta strandi431 – 44010
    Helixi443 – 4453
    Turni446 – 4483
    Beta strandi453 – 4619
    Beta strandi470 – 4734
    Helixi475 – 4773
    Beta strandi479 – 4824
    Helixi485 – 4873
    Helixi495 – 4973
    Turni501 – 5033
    Helixi508 – 5103
    Helixi512 – 5143
    Beta strandi515 – 5228
    Beta strandi528 – 53710
    Beta strandi543 – 5453
    Beta strandi549 – 5579
    Beta strandi573 – 5797

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1KNBX-ray1.70A386-581[»]
    4ATZX-ray1.95A/B/C387-581[»]
    ProteinModelPortaliP11818.
    SMRiP11818. Positions 321-581.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP11818.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati45 – 5915Shaft 1Add
    BLAST
    Repeati60 – 7516Shaft 2Add
    BLAST
    Repeati76 – 9520Shaft 3Add
    BLAST
    Repeati96 – 10914Shaft 4Add
    BLAST
    Repeati110 – 12415Shaft 5Add
    BLAST
    Repeati125 – 13915Shaft 6Add
    BLAST
    Repeati140 – 15415Shaft 7Add
    BLAST
    Repeati155 – 17016Shaft 8Add
    BLAST
    Repeati171 – 18515Shaft 9Add
    BLAST
    Repeati186 – 20015Shaft 10Add
    BLAST
    Repeati201 – 21717Shaft 11Add
    BLAST
    Repeati218 – 23215Shaft 12Add
    BLAST
    Repeati233 – 24816Shaft 13Add
    BLAST
    Repeati249 – 26517Shaft 14Add
    BLAST
    Repeati266 – 27914Shaft 15Add
    BLAST
    Repeati280 – 29617Shaft 16Add
    BLAST
    Repeati297 – 31620Shaft 17Add
    BLAST
    Repeati317 – 33317Shaft 18Add
    BLAST
    Repeati334 – 35421Shaft 19Add
    BLAST
    Repeati355 – 37016Shaft 20Add
    BLAST
    Repeati371 – 38616Shaft 21Add
    BLAST
    Repeati387 – 3926Shaft 22

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 4444Tail (penton base attachment)By similarityAdd
    BLAST
    Regioni45 – 392348Shaft regionBy similarityAdd
    BLAST
    Regioni393 – 3986SpacerBy similarity
    Regioni399 – 581183HeadBy similarityAdd
    BLAST

    Domaini

    The tail region anchors the fiber to penton base capsomers, whereas the shaft, built from several repeated motifs, allows the knob to protrude from the virion.By similarity

    Sequence similaritiesi

    Belongs to the adenoviridae fiber family.Curated
    Contains 22 Shaft repeats.Curated

    Keywords - Domaini

    Repeat

    Family and domain databases

    Gene3Di2.10.25.20. 3 hits.
    2.60.90.10. 1 hit.
    InterProiIPR000931. Adeno_fibre.
    IPR000978. Adeno_fibre_knob.
    IPR000939. Adenobir_fibre_prot_rpt/shaft.
    IPR008982. Adenovirus_pIV-rel_att.
    IPR009013. Attachment_protein_shaft_dom.
    [Graphical view]
    PfamiPF00541. Adeno_knob. 1 hit.
    PF00608. Adeno_shaft. 6 hits.
    [Graphical view]
    PRINTSiPR00307. ADENOVSFIBRE.
    SUPFAMiSSF49835. SSF49835. 1 hit.
    SSF51225. SSF51225. 4 hits.

    Sequencei

    Sequence statusi: Complete.

    P11818-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRARPSEDT FNPVYPYDTE TGPPTVPFLT PPFVSPNGFQ ESPPGVLSLR    50
    LSEPLVTSNG MLALKMGNGL SLDEAGNLTS QNVTTVSPPL KKTKSNINLE 100
    ISAPLTVTSE ALTVAAAAPL MVAGNTLTMQ SQAPLTVHDS KLSIATQGPL 150
    TVSEGKLALQ TSGPLTTTDS STLTITASPP LTTATGSLGI DLKEPIYTQN 200
    GKLGLKYGAP LHVTDDLNTL TVATGPGVTI NNTSLQTKVT GALGFDSQGN 250
    MQLNVAGGLR IDSQNRRLIL DVSYPFDAQN QLNLRLGQGP LFINSAHNLD 300
    INYNKGLYLF TASNNSKKLE VNLSTAKGLM FDATAIAINA GDGLEFGSPN 350
    APNTNPLKTK IGHGLEFDSN KAMVPKLGTG LSFDSTGAIT VGNKNNDKLT 400
    LWTTPAPSPN CRLNAEKDAK LTLVLTKCGS QILATVSVLA VKGSLAPISG 450
    TVQSAHLIIR FDENGVLLNN SFLDPEYWNF RNGDLTEGTA YTNAVGFMPN 500
    LSAYPKSHGK TAKSNIVSQV YLNGDKTKPV TLTITLNGTQ ETGDTTPSAY 550
    SMSFSWDWSG HNYINEIFAT SSYTFSYIAQ E 581
    Length:581
    Mass (Da):61,585
    Last modified:October 1, 1989 - v1
    Checksum:iC3FDDFCF9E5E599F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73260 Genomic DNA. No translation available.
    M18369 Genomic DNA. Translation: AAA42504.1.
    PIRiA27404. ERADF5.
    RefSeqiAP_000226.1. AC_000008.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73260 Genomic DNA. No translation available.
    M18369 Genomic DNA. Translation: AAA42504.1 .
    PIRi A27404. ERADF5.
    RefSeqi AP_000226.1. AC_000008.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1KNB X-ray 1.70 A 386-581 [» ]
    4ATZ X-ray 1.95 A/B/C 387-581 [» ]
    ProteinModelPortali P11818.
    SMRi P11818. Positions 321-581.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    UniCarbKBi P11818.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P11818.

    Family and domain databases

    Gene3Di 2.10.25.20. 3 hits.
    2.60.90.10. 1 hit.
    InterProi IPR000931. Adeno_fibre.
    IPR000978. Adeno_fibre_knob.
    IPR000939. Adenobir_fibre_prot_rpt/shaft.
    IPR008982. Adenovirus_pIV-rel_att.
    IPR009013. Attachment_protein_shaft_dom.
    [Graphical view ]
    Pfami PF00541. Adeno_knob. 1 hit.
    PF00608. Adeno_shaft. 6 hits.
    [Graphical view ]
    PRINTSi PR00307. ADENOVSFIBRE.
    SUPFAMi SSF49835. SSF49835. 1 hit.
    SSF51225. SSF51225. 4 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The sequence of adenovirus fiber: similarities and differences between serotypes 2 and 5."
      Chroboczek J., Jacrot B.
      Virology 161:549-554(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The sequence of the genome of adenovirus type 5 and its comparison with the genome of adenovirus type 2."
      Chroboczek J., Bieber F., Jacrot B.
      Virology 186:280-285(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "De novo derivation of proteomes from transcriptomes for transcript and protein identification."
      Evans V.C., Barker G., Heesom K.J., Fan J., Bessant C., Matthews D.A.
      Nat. Methods 9:1207-1211(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Relative accessibility of N-acetylglucosamine in trimers of the adenovirus types 2 and 5 fiber proteins."
      Mullis K.G., Haltiwanger R.S., Hart G.W., Marchase R.B., Engler J.A.
      J. Virol. 64:5317-5323(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION.
    5. Cited for: REVIEW.
    6. "Crystal structure of the receptor-binding domain of adenovirus type 5 fiber protein at 1.7-A resolution."
      Xia D., Henry L.J., Gerard R.D., Deisenhofer J.
      Structure 2:1259-1270(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 386-581.

    Entry informationi

    Entry nameiSPIKE_ADE05
    AccessioniPrimary (citable) accession number: P11818
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell By similarity.By similarity

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3