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P11801

- KPSH1_HUMAN

UniProt

P11801 - KPSH1_HUMAN

Protein

Serine/threonine-protein kinase H1

Gene

PSKH1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 148 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activity depends on Ca2+ concentration.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei127 – 1271ATPPROSITE-ProRule annotation
    Active sitei218 – 2181Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi104 – 1129ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine kinase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.1. 2681.
    SignaLinkiP11801.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase H1 (EC:2.7.11.1)
    Alternative name(s):
    Protein serine kinase H1
    Short name:
    PSK-H1
    Gene namesi
    Name:PSKH1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:9529. PSKH1.

    Subcellular locationi

    Golgi apparatus. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Nucleus speckle. Endoplasmic reticulum membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Cytoplasm
    Note: Localized in the brefeldin A-sensitive Golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and more diffusely in the cytoplasm. Found to concentrate in splicing factor compartments (SFCs) within the nucleus of interphase cells. The acylation-negative form may be only cytoplasmic and nuclear. Acylation seems to allow the sequestering to the intracellular membranes. Myristoylation may mediate targeting to the intracellular non-Golgi membranes and palmitoylation may mediate the targeting to the Golgi membranes. Dual acylation is required to stabilize the interaction with Golgi membranes.

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. Golgi apparatus Source: UniProtKB-SubCell
    3. microtubule organizing center Source: UniProtKB-SubCell
    4. nuclear speck Source: UniProtKB-SubCell
    5. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi218 – 2181D → A: Loss of autophosphorylation. 1 Publication

    Organism-specific databases

    PharmGKBiPA33874.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedCurated
    Chaini2 – 424423Serine/threonine-protein kinase H1PRO_0000086167Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycine1 Publication
    Lipidationi3 – 31S-palmitoyl cysteine1 Publication
    Modified residuei380 – 3801Phosphoserine; by autocatalysisSequence Analysis
    Modified residuei381 – 3811Phosphoserine; by autocatalysisSequence Analysis

    Post-translational modificationi

    Autophosphorylated on serine residues.1 Publication
    Myristoylated. Required for membrane association. Prerequisite for palmitoylation to occur.1 Publication
    Palmitoylated.1 Publication

    Keywords - PTMi

    Lipoprotein, Myristate, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiP11801.
    PRIDEiP11801.

    PTM databases

    PhosphoSiteiP11801.

    Expressioni

    Tissue specificityi

    Expressed in all tissues and cell lines tested with the highest level of abundance in testis.1 Publication

    Gene expression databases

    BgeeiP11801.
    CleanExiHS_PSKH1.
    GenevestigatoriP11801.

    Organism-specific databases

    HPAiHPA053927.

    Interactioni

    Subunit structurei

    Homodimer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HSP90AB1P082382EBI-3922781,EBI-352572

    Protein-protein interaction databases

    BioGridi111654. 4 interactions.
    IntActiP11801. 2 interactions.
    STRINGi9606.ENSP00000291041.

    Structurei

    3D structure databases

    ProteinModelPortaliP11801.
    SMRiP11801. Positions 98-382.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini98 – 355258Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233016.
    HOVERGENiHBG108055.
    InParanoidiP11801.
    KOiK08808.
    OMAiAASQCAN.
    OrthoDBiEOG7WHH9K.
    PhylomeDBiP11801.
    TreeFamiTF314166.

    Family and domain databases

    InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24347. PTHR24347. 1 hit.
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P11801-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGCGTSKVLP EPPKDVQLDL VKKVEPFSGT KSDVYKHFIT EVDSVGPVKA    50
    GFPAASQYAH PCPGPPTAGH TEPPSEPPRR ARVAKYRAKF DPRVTAKYDI 100
    KALIGRGSFS RVVRVEHRAT RQPYAIKMIE TKYREGREVC ESELRVLRRV 150
    RHANIIQLVE VFETQERVYM VMELATGGEL FDRIIAKGSF TERDATRVLQ 200
    MVLDGVRYLH ALGITHRDLK PENLLYYHPG TDSKIIITDF GLASARKKGD 250
    DCLMKTTCGT PEYIAPEVLV RKPYTNSVDM WALGVIAYIL LSGTMPFEDD 300
    NRTRLYRQIL RGKYSYSGEP WPSVSNLAKD FIDRLLTVDP GARMTALQAL 350
    RHPWVVSMAA SSSMKNLHRS ISQNLLKRAS SRCQSTKSAQ STRSSRSTRS 400
    NKSRRVRERE LRELNLRYQQ QYNG 424
    Length:424
    Mass (Da):48,035
    Last modified:January 23, 2007 - v4
    Checksum:iB357757DDC12388D
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti301 – 3011N → S.1 Publication
    Corresponds to variant rs35552721 [ dbSNP | Ensembl ].
    VAR_040614

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ272212 mRNA. Translation: CAB91984.1.
    BC062616 mRNA. Translation: AAH62616.1.
    M14504 mRNA. Translation: AAA36519.1.
    CCDSiCCDS10851.1.
    PIRiB26368.
    I38138.
    RefSeqiNP_006733.1. NM_006742.2.
    UniGeneiHs.513683.

    Genome annotation databases

    EnsembliENST00000291041; ENSP00000291041; ENSG00000159792.
    GeneIDi5681.
    KEGGihsa:5681.
    UCSCiuc002euv.3. human.

    Polymorphism databases

    DMDMi17379144.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ272212 mRNA. Translation: CAB91984.1 .
    BC062616 mRNA. Translation: AAH62616.1 .
    M14504 mRNA. Translation: AAA36519.1 .
    CCDSi CCDS10851.1.
    PIRi B26368.
    I38138.
    RefSeqi NP_006733.1. NM_006742.2.
    UniGenei Hs.513683.

    3D structure databases

    ProteinModelPortali P11801.
    SMRi P11801. Positions 98-382.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111654. 4 interactions.
    IntActi P11801. 2 interactions.
    STRINGi 9606.ENSP00000291041.

    PTM databases

    PhosphoSitei P11801.

    Polymorphism databases

    DMDMi 17379144.

    Proteomic databases

    PaxDbi P11801.
    PRIDEi P11801.

    Protocols and materials databases

    DNASUi 5681.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000291041 ; ENSP00000291041 ; ENSG00000159792 .
    GeneIDi 5681.
    KEGGi hsa:5681.
    UCSCi uc002euv.3. human.

    Organism-specific databases

    CTDi 5681.
    GeneCardsi GC16P067927.
    HGNCi HGNC:9529. PSKH1.
    HPAi HPA053927.
    MIMi 177015. gene.
    neXtProti NX_P11801.
    PharmGKBi PA33874.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233016.
    HOVERGENi HBG108055.
    InParanoidi P11801.
    KOi K08808.
    OMAi AASQCAN.
    OrthoDBi EOG7WHH9K.
    PhylomeDBi P11801.
    TreeFami TF314166.

    Enzyme and pathway databases

    BRENDAi 2.7.11.1. 2681.
    SignaLinki P11801.

    Miscellaneous databases

    ChiTaRSi PSKH1. human.
    GeneWikii PSKH1.
    GenomeRNAii 5681.
    NextBioi 22058.
    PROi P11801.
    SOURCEi Search...

    Gene expression databases

    Bgeei P11801.
    CleanExi HS_PSKH1.
    Genevestigatori P11801.

    Family and domain databases

    InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24347. PTHR24347. 1 hit.
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of PSKH1, a novel human protein serine kinase with centrosomal, Golgi, and nuclear localization."
      Brede G., Solheim J., Troen G., Prydz H.
      Genomics 70:82-92(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PHOSPHORYLATION, HOMODIMERIZATION, MUTAGENESIS OF ASP-218.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Colon.
    3. "Homology probing: identification of cDNA clones encoding members of the protein-serine kinase family."
      Hanks S.K.
      Proc. Natl. Acad. Sci. U.S.A. 84:388-392(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 199-348.
    4. "PSKH1, a novel splice factor compartment-associated serine kinase."
      Brede G., Solheim J., Prydz H.
      Nucleic Acids Res. 30:5301-5309(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Mutants of the protein serine kinase PSKH1 disassemble the Golgi apparatus."
      Brede G., Solheim J., Stang E., Prydz H.
      Exp. Cell Res. 291:299-312(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: MYRISTOYLATION AT GLY-2, PALMITOYLATION AT CYS-3, SUBCELLULAR LOCATION.
    6. "Patterns of somatic mutation in human cancer genomes."
      Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
      , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
      Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT [LARGE SCALE ANALYSIS] SER-301.

    Entry informationi

    Entry nameiKPSH1_HUMAN
    AccessioniPrimary (citable) accession number: P11801
    Secondary accession number(s): Q9NY19
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 148 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3