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P11801 (KPSH1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 145. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase H1

EC=2.7.11.1
Alternative name(s):
Protein serine kinase H1
Short name=PSK-H1
Gene names
Name:PSKH1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length424 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing. Ref.4

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activity depends on Ca2+ concentration.

Subunit structure

Homodimer. Ref.1

Subcellular location

Golgi apparatus. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Nucleus speckle. Endoplasmic reticulum membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Cytoplasm. Note: Localized in the brefeldin A-sensitive Golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and more diffusely in the cytoplasm. Found to concentrate in splicing factor compartments (SFCs) within the nucleus of interphase cells. The acylation-negative form may be only cytoplasmic and nuclear. Acylation seems to allow the sequestering to the intracellular membranes. Myristoylation may mediate targeting to the intracellular non-Golgi membranes and palmitoylation may mediate the targeting to the Golgi membranes. Dual acylation is required to stabilize the interaction with Golgi membranes. Ref.1 Ref.5

Tissue specificity

Expressed in all tissues and cell lines tested with the highest level of abundance in testis. Ref.1

Post-translational modification

Autophosphorylated on serine residues. Ref.1

Myristoylated. Required for membrane association. Prerequisite for palmitoylation to occur. Ref.5

Palmitoylated. Ref.5

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HSP90AB1P082382EBI-3922781,EBI-352572

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Probable
Chain2 – 424423Serine/threonine-protein kinase H1
PRO_0000086167

Regions

Domain98 – 355258Protein kinase
Nucleotide binding104 – 1129ATP By similarity

Sites

Active site2181Proton acceptor By similarity
Binding site1271ATP By similarity

Amino acid modifications

Modified residue3801Phosphoserine; by autocatalysis Potential
Modified residue3811Phosphoserine; by autocatalysis Potential
Lipidation21N-myristoyl glycine Ref.5
Lipidation31S-palmitoyl cysteine Ref.5

Natural variations

Natural variant3011N → S. Ref.6
Corresponds to variant rs35552721 [ dbSNP | Ensembl ].
VAR_040614

Experimental info

Mutagenesis2181D → A: Loss of autophosphorylation. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P11801 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: B357757DDC12388D

FASTA42448,035
        10         20         30         40         50         60 
MGCGTSKVLP EPPKDVQLDL VKKVEPFSGT KSDVYKHFIT EVDSVGPVKA GFPAASQYAH 

        70         80         90        100        110        120 
PCPGPPTAGH TEPPSEPPRR ARVAKYRAKF DPRVTAKYDI KALIGRGSFS RVVRVEHRAT 

       130        140        150        160        170        180 
RQPYAIKMIE TKYREGREVC ESELRVLRRV RHANIIQLVE VFETQERVYM VMELATGGEL 

       190        200        210        220        230        240 
FDRIIAKGSF TERDATRVLQ MVLDGVRYLH ALGITHRDLK PENLLYYHPG TDSKIIITDF 

       250        260        270        280        290        300 
GLASARKKGD DCLMKTTCGT PEYIAPEVLV RKPYTNSVDM WALGVIAYIL LSGTMPFEDD 

       310        320        330        340        350        360 
NRTRLYRQIL RGKYSYSGEP WPSVSNLAKD FIDRLLTVDP GARMTALQAL RHPWVVSMAA 

       370        380        390        400        410        420 
SSSMKNLHRS ISQNLLKRAS SRCQSTKSAQ STRSSRSTRS NKSRRVRERE LRELNLRYQQ 


QYNG 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of PSKH1, a novel human protein serine kinase with centrosomal, Golgi, and nuclear localization."
Brede G., Solheim J., Troen G., Prydz H.
Genomics 70:82-92(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PHOSPHORYLATION, HOMODIMERIZATION, MUTAGENESIS OF ASP-218.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon.
[3]"Homology probing: identification of cDNA clones encoding members of the protein-serine kinase family."
Hanks S.K.
Proc. Natl. Acad. Sci. U.S.A. 84:388-392(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 199-348.
[4]"PSKH1, a novel splice factor compartment-associated serine kinase."
Brede G., Solheim J., Prydz H.
Nucleic Acids Res. 30:5301-5309(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Mutants of the protein serine kinase PSKH1 disassemble the Golgi apparatus."
Brede G., Solheim J., Stang E., Prydz H.
Exp. Cell Res. 291:299-312(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: MYRISTOYLATION AT GLY-2, PALMITOYLATION AT CYS-3, SUBCELLULAR LOCATION.
[6]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] SER-301.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ272212 mRNA. Translation: CAB91984.1.
BC062616 mRNA. Translation: AAH62616.1.
M14504 mRNA. Translation: AAA36519.1.
PIRB26368.
I38138.
RefSeqNP_006733.1. NM_006742.2.
UniGeneHs.513683.

3D structure databases

ProteinModelPortalP11801.
SMRP11801. Positions 98-382.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111654. 4 interactions.
IntActP11801. 2 interactions.
STRING9606.ENSP00000291041.

PTM databases

PhosphoSiteP11801.

Polymorphism databases

DMDM17379144.

Proteomic databases

PaxDbP11801.
PRIDEP11801.

Protocols and materials databases

DNASU5681.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000291041; ENSP00000291041; ENSG00000159792.
GeneID5681.
KEGGhsa:5681.
UCSCuc002euv.3. human.

Organism-specific databases

CTD5681.
GeneCardsGC16P067927.
HGNCHGNC:9529. PSKH1.
HPAHPA053927.
MIM177015. gene.
neXtProtNX_P11801.
PharmGKBPA33874.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233016.
HOVERGENHBG108055.
InParanoidP11801.
KOK08808.
OMAAASQCAN.
OrthoDBEOG7WHH9K.
PhylomeDBP11801.
TreeFamTF314166.

Enzyme and pathway databases

BRENDA2.7.11.1. 2681.
SignaLinkP11801.

Gene expression databases

BgeeP11801.
CleanExHS_PSKH1.
GenevestigatorP11801.

Family and domain databases

InterProIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERPTHR24347. PTHR24347. 1 hit.
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPSKH1. human.
GeneWikiPSKH1.
GenomeRNAi5681.
NextBio22058.
PROP11801.
SOURCESearch...

Entry information

Entry nameKPSH1_HUMAN
AccessionPrimary (citable) accession number: P11801
Secondary accession number(s): Q9NY19
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: March 19, 2014
This is version 145 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM