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Protein

Serine/threonine-protein kinase H1

Gene

PSKH1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activity depends on Ca2+ concentration.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei127 – 1271ATPPROSITE-ProRule annotation
Active sitei218 – 2181Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi104 – 1129ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 2681.
SignaLinkiP11801.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase H1 (EC:2.7.11.1)
Alternative name(s):
Protein serine kinase H1
Short name:
PSK-H1
Gene namesi
Name:PSKH1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:9529. PSKH1.

Subcellular locationi

  • Golgi apparatus
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome
  • Nucleus speckle
  • Endoplasmic reticulum membrane; Lipid-anchor
  • Cell membrane; Lipid-anchor
  • Cytoplasm

  • Note: Localized in the brefeldin A-sensitive Golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and more diffusely in the cytoplasm. Found to concentrate in splicing factor compartments (SFCs) within the nucleus of interphase cells. The acylation-negative form may be only cytoplasmic and nuclear. Acylation seems to allow the sequestering to the intracellular membranes. Myristoylation may mediate targeting to the intracellular non-Golgi membranes and palmitoylation may mediate the targeting to the Golgi membranes. Dual acylation is required to stabilize the interaction with Golgi membranes.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi218 – 2181D → A: Loss of autophosphorylation. 1 Publication

Organism-specific databases

PharmGKBiPA33874.

Polymorphism and mutation databases

BioMutaiPSKH1.
DMDMi17379144.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedCurated
Chaini2 – 424423Serine/threonine-protein kinase H1PRO_0000086167Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication
Lipidationi3 – 31S-palmitoyl cysteine1 Publication
Modified residuei380 – 3801Phosphoserine; by autocatalysisSequence Analysis
Modified residuei381 – 3811Phosphoserine; by autocatalysisSequence Analysis

Post-translational modificationi

Autophosphorylated on serine residues.1 Publication
Myristoylated. Required for membrane association. Prerequisite for palmitoylation to occur.1 Publication
Palmitoylated.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP11801.
PRIDEiP11801.

PTM databases

PhosphoSiteiP11801.

Expressioni

Tissue specificityi

Expressed in all tissues and cell lines tested with the highest level of abundance in testis.1 Publication

Gene expression databases

BgeeiP11801.
CleanExiHS_PSKH1.
ExpressionAtlasiP11801. baseline and differential.
GenevisibleiP11801. HS.

Organism-specific databases

HPAiHPA053927.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
HSP90AB1P082382EBI-3922781,EBI-352572

Protein-protein interaction databases

BioGridi111654. 5 interactions.
IntActiP11801. 2 interactions.
STRINGi9606.ENSP00000291041.

Structurei

3D structure databases

ProteinModelPortaliP11801.
SMRiP11801. Positions 97-404.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini98 – 355258Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118944.
HOGENOMiHOG000233016.
HOVERGENiHBG108055.
InParanoidiP11801.
KOiK08808.
OMAiAASQCAN.
OrthoDBiEOG7WHH9K.
PhylomeDBiP11801.
TreeFamiTF314166.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11801-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCGTSKVLP EPPKDVQLDL VKKVEPFSGT KSDVYKHFIT EVDSVGPVKA
60 70 80 90 100
GFPAASQYAH PCPGPPTAGH TEPPSEPPRR ARVAKYRAKF DPRVTAKYDI
110 120 130 140 150
KALIGRGSFS RVVRVEHRAT RQPYAIKMIE TKYREGREVC ESELRVLRRV
160 170 180 190 200
RHANIIQLVE VFETQERVYM VMELATGGEL FDRIIAKGSF TERDATRVLQ
210 220 230 240 250
MVLDGVRYLH ALGITHRDLK PENLLYYHPG TDSKIIITDF GLASARKKGD
260 270 280 290 300
DCLMKTTCGT PEYIAPEVLV RKPYTNSVDM WALGVIAYIL LSGTMPFEDD
310 320 330 340 350
NRTRLYRQIL RGKYSYSGEP WPSVSNLAKD FIDRLLTVDP GARMTALQAL
360 370 380 390 400
RHPWVVSMAA SSSMKNLHRS ISQNLLKRAS SRCQSTKSAQ STRSSRSTRS
410 420
NKSRRVRERE LRELNLRYQQ QYNG
Length:424
Mass (Da):48,035
Last modified:January 23, 2007 - v4
Checksum:iB357757DDC12388D
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti301 – 3011N → S.1 Publication
Corresponds to variant rs35552721 [ dbSNP | Ensembl ].
VAR_040614

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ272212 mRNA. Translation: CAB91984.1.
BC062616 mRNA. Translation: AAH62616.1.
M14504 mRNA. Translation: AAA36519.1.
CCDSiCCDS10851.1.
PIRiB26368.
I38138.
RefSeqiNP_006733.1. NM_006742.2.
UniGeneiHs.513683.

Genome annotation databases

EnsembliENST00000291041; ENSP00000291041; ENSG00000159792.
GeneIDi5681.
KEGGihsa:5681.
UCSCiuc002euv.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ272212 mRNA. Translation: CAB91984.1.
BC062616 mRNA. Translation: AAH62616.1.
M14504 mRNA. Translation: AAA36519.1.
CCDSiCCDS10851.1.
PIRiB26368.
I38138.
RefSeqiNP_006733.1. NM_006742.2.
UniGeneiHs.513683.

3D structure databases

ProteinModelPortaliP11801.
SMRiP11801. Positions 97-404.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111654. 5 interactions.
IntActiP11801. 2 interactions.
STRINGi9606.ENSP00000291041.

PTM databases

PhosphoSiteiP11801.

Polymorphism and mutation databases

BioMutaiPSKH1.
DMDMi17379144.

Proteomic databases

PaxDbiP11801.
PRIDEiP11801.

Protocols and materials databases

DNASUi5681.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000291041; ENSP00000291041; ENSG00000159792.
GeneIDi5681.
KEGGihsa:5681.
UCSCiuc002euv.3. human.

Organism-specific databases

CTDi5681.
GeneCardsiGC16P067927.
HGNCiHGNC:9529. PSKH1.
HPAiHPA053927.
MIMi177015. gene.
neXtProtiNX_P11801.
PharmGKBiPA33874.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118944.
HOGENOMiHOG000233016.
HOVERGENiHBG108055.
InParanoidiP11801.
KOiK08808.
OMAiAASQCAN.
OrthoDBiEOG7WHH9K.
PhylomeDBiP11801.
TreeFamiTF314166.

Enzyme and pathway databases

BRENDAi2.7.11.1. 2681.
SignaLinkiP11801.

Miscellaneous databases

ChiTaRSiPSKH1. human.
GeneWikiiPSKH1.
GenomeRNAii5681.
NextBioi22058.
PROiP11801.
SOURCEiSearch...

Gene expression databases

BgeeiP11801.
CleanExiHS_PSKH1.
ExpressionAtlasiP11801. baseline and differential.
GenevisibleiP11801. HS.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of PSKH1, a novel human protein serine kinase with centrosomal, Golgi, and nuclear localization."
    Brede G., Solheim J., Troen G., Prydz H.
    Genomics 70:82-92(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PHOSPHORYLATION, HOMODIMERIZATION, MUTAGENESIS OF ASP-218.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon.
  3. "Homology probing: identification of cDNA clones encoding members of the protein-serine kinase family."
    Hanks S.K.
    Proc. Natl. Acad. Sci. U.S.A. 84:388-392(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 199-348.
  4. "PSKH1, a novel splice factor compartment-associated serine kinase."
    Brede G., Solheim J., Prydz H.
    Nucleic Acids Res. 30:5301-5309(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Mutants of the protein serine kinase PSKH1 disassemble the Golgi apparatus."
    Brede G., Solheim J., Stang E., Prydz H.
    Exp. Cell Res. 291:299-312(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: MYRISTOYLATION AT GLY-2, PALMITOYLATION AT CYS-3, SUBCELLULAR LOCATION.
  6. "Patterns of somatic mutation in human cancer genomes."
    Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
    , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
    Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT [LARGE SCALE ANALYSIS] SER-301.

Entry informationi

Entry nameiKPSH1_HUMAN
AccessioniPrimary (citable) accession number: P11801
Secondary accession number(s): Q9NY19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 155 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.