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Protein

Myosin light chain kinase, smooth muscle

Gene

Mylk

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates a specific serine in the N-terminus of a myosin light chain, which leads to the formation of calmodulin/MLCK signal transduction complexes which allow selective transduction of calcium signals.

Catalytic activityi

ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Activated by phosphorylation on Tyr-478. Isoforms which lack this tyrosine residue are not regulated in this way. All catalytically active isoforms require binding to calcium and calmodulin for activation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1482ATPPROSITE-ProRule annotation1
Active sitei1574Proton acceptor1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi1459 – 1467ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.18. 1306.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light chain kinase, smooth muscle (EC:2.7.11.18)
Short name:
MLCK
Alternative name(s):
Telokin
Cleaved into the following chain:
Gene namesi
Name:Mylk
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

  • Cytoplasmcytosol 1 Publication
  • Membrane 1 Publication

  • Note: Telokin is cytosolic and can translocate to the membrane upon stimulation.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1762S → A: Decreases membrane translocation. 1 Publication1
Mutagenesisi1768S → A: Decreases membrane translocation. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL3062.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000243611 – 1906Myosin light chain kinase, smooth muscleAdd BLAST1906
ChainiPRO_00004248671 – 1901Myosin light chain kinase, smooth muscle, deglutamylated formAdd BLAST1901

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei478Phosphotyrosine1 Publication1
Modified residuei1748Phosphoserine1 Publication1
Modified residuei1762Phosphoserine; by PKG1 Publication1
Modified residuei1768Phosphoserine; by MAPK1 Publication1

Post-translational modificationi

The C-terminus is deglutamylated, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The C-terminus is variable, with one to five C-terminal glutamyl residues being removed producing five forms differring in their number of C-terminal glutamyl residues.
Acetylated.1 Publication
Phosphorylation of telokin by PKG has no significant effect on its myosin binding activity, but promotes translocation to the membrane.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP11799.
PRIDEiP11799.

PTM databases

iPTMnetiP11799.

Expressioni

Tissue specificityi

Isoform telokin is expressed in gizzard, heart, lung, intestine, and skeletal muscle although the levels of the expression in the latter were much less than that in the gizzard.

Interactioni

Subunit structurei

All isoforms including Telokin bind calmodulin.1 Publication

Protein-protein interaction databases

BioGridi676694. 1 interactor.
IntActiP11799. 3 interactors.
STRINGi9031.ENSGALP00000019112.

Chemistry databases

BindingDBiP11799.

Structurei

Secondary structure

11906
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1731 – 1747Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CDLX-ray2.00E/F/G/H1730-1749[»]
1QS7X-ray1.80B/D1731-1749[»]
1QTXX-ray1.65B1731-1749[»]
1VRKX-ray1.90B1731-1749[»]
2O5GX-ray1.08B1730-1748[»]
3EVUX-ray1.75A1731-1749[»]
3EVVX-ray2.60A1731-1749[»]
3O77X-ray2.35A1730-1749[»]
3O78X-ray2.60A/B1731-1749[»]
ProteinModelPortaliP11799.
SMRiP11799.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11799.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 117Ig-like C2-type 1Add BLAST90
Domaini156 – 244Ig-like C2-type 2Add BLAST89
Domaini429 – 517Ig-like C2-type 3Add BLAST89
Domaini521 – 613Ig-like C2-type 4Add BLAST93
Domaini637 – 725Ig-like C2-type 5Add BLAST89
Repeati660 – 676IIA-1Add BLAST17
Repeati693 – 708IIB-1Add BLAST16
Domaini735 – 830Ig-like C2-type 6Add BLAST96
Repeati758 – 774IIA-2Add BLAST17
Repeati791 – 807IIB-2Add BLAST17
Repeati970 – 987III-1Add BLAST18
Repeati999 – 1016III-2Add BLAST18
Repeati1061 – 1078III-3Add BLAST18
Domaini1084 – 1172Ig-like C2-type 7Add BLAST89
Repeati1107 – 1123IIA-3Add BLAST17
Repeati1140 – 1156IIB-3Add BLAST17
Repeati1209 – 1226III-4Add BLAST18
Domaini1225 – 1313Ig-like C2-type 8Add BLAST89
Repeati1281 – 1297IIB-4Add BLAST17
Domaini1321 – 1414Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST94
Domaini1453 – 1708Protein kinasePROSITE-ProRule annotationAdd BLAST256
Domaini1794 – 1885Ig-like C2-type 9Add BLAST92
Repeati1817 – 1833IIA-4Add BLAST17
Repeati1851 – 1866IIB-5Add BLAST16

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni660 – 18334 X repeats, motif IIAAdd BLAST1174
Regioni693 – 18665 X repeats, motif IIBAdd BLAST1174
Regioni970 – 12264 X repeats, motif IIIAdd BLAST257
Regioni1317 – 1364Motif IAAdd BLAST48
Regioni1385 – 1402Motif IBAdd BLAST18
Regioni1700 – 1763Calmodulin-bindingAdd BLAST64
Regioni1716 – 1728Calmodulin autoinhibition (AM13) regionSequence analysisAdd BLAST13
Regioni1730 – 1749Calmodulin recognition (RS20) regionSequence analysisAdd BLAST20

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1896 – 1906Poly-GluAdd BLAST11

Sequence similaritiesi

Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat

Phylogenomic databases

eggNOGiKOG0613. Eukaryota.
ENOG410XQFD. LUCA.
HOGENOMiHOG000049287.
HOVERGENiHBG052551.
PhylomeDBiP11799.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 10 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR015725. Telokin/Myosin_light_ch_kin.
[Graphical view]
PANTHERiPTHR10489:SF700. PTHR10489:SF700. 4 hits.
PfamiPF00041. fn3. 1 hit.
PF07679. I-set. 9 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
SM00409. IG. 9 hits.
SM00408. IGc2. 9 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 9 hits.
SSF49265. SSF49265. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50853. FN3. 1 hit.
PS50835. IG_LIKE. 9 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative initiation. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P11799-1) [UniParc]FASTAAdd to basket
Also known as: MLCK-108, Smooth-muscle

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDVKLVTST RVSKTSLTLS PSVPAEAPAF TLPPRNIRVQ LGATARFEGK
60 70 80 90 100
VRGYPEPQIT WYRNGHPLPE GDHYVVDHSI RGIFSLVIKG VQEGDSGKYT
110 120 130 140 150
CEAANDGGVR QVTVELTVEG NSLKKYSLPS SAKTPGGRLS VPPVEHRPSI
160 170 180 190 200
WGESPPKFAT KPNRVVVREG QTGRFSCKIT GRPQPQVTWT KGDIHLQQNE
210 220 230 240 250
RFNMFEKTGI QYLEIQNVQL ADAGIYTCTV VNSAGKASVS AELTVQGPDK
260 270 280 290 300
TDTHAQPLCM PPKPTTLATK AIENSDFKQA TSNGIAKELK STSTELMVET
310 320 330 340 350
KDRLSAKKET FYTSREAKDG KQGQNQEANA VPLQESRGTK GPQVLQKTSS
360 370 380 390 400
TITLQAVKAQ PEPKAEPQTT FIRQAEDRKR TVQPLMTTTT QENPSLTGQV
410 420 430 440 450
SPRSRETENR AGVRKSVKEE KREPLGIPPQ FESRPQSLEA SEGQEIKFKS
460 470 480 490 500
KVSGKPKPDV EWFKEGVPIK TGEGIQIYEE DGTHCLWLKK ACLGDSGSYS
510 520 530 540 550
CAAFNPRGQT STSWLLTVKR PKVEEVAPCF SSVLKGCTVS EGQDFVLQCY
560 570 580 590 600
VGGVPVPEIT WLLNEQPIQY AHSTFEAGVA KLTVQDALPE DDGIYTCLAE
610 620 630 640 650
NNAGRASCSA QVTVKEKKSS KKAEGTQAAK LNKTFAPIFL KGLTDLKVMD
660 670 680 690 700
GSQVIMTVEV SANPCPEIIW LHNGKEIQET EDFHFEKKGN EYSLYIQEVF
710 720 730 740 750
PEDTGKYTCE AWNELGETQT QATLTVQEPQ DGIQPWFISK PRSVTAAAGQ
760 770 780 790 800
NVLISCAIAG DPFPTVHWFK DGQEITPGTG CEILQNEDIF TLILRNVQSR
810 820 830 840 850
HAGQYEIQLR NQVGECSCQV SLMLRESSAS RAEMLRDGRE SASSGERRDG
860 870 880 890 900
GNYGALTFGR TSGFKKSSSE TRAAEEEQED VRGVLKRRVE TREHTEESLR
910 920 930 940 950
QQEAEQLDFR DILGKKVSTK SFSEEDLKEI PAEQMDFRAN LQRQVKPKTL
960 970 980 990 1000
SEEERKVHAP QQVDFRSVLA KKGTPKTPLP EKVPPPKPAV TDFRSVLGAK
1010 1020 1030 1040 1050
KKPPAENGSA STPAPNARAG SEAQNATPNS EAPAPKPVVK KEEKNDRKCE
1060 1070 1080 1090 1100
HGCAVVDGGI IGKKAENKPA ASKPTPPPSK GTAPSFTEKL QDAKVADGEK
1110 1120 1130 1140 1150
LVLQCRISSD PPASVSWTLD SKAIKSSKSI VISQEGTLCS LTIEKVMPED
1160 1170 1180 1190 1200
GGEYKCIAEN AAGKAECACK VLVEDTSSTK AAKPAEKKTK KPKTTLPPVL
1210 1220 1230 1240 1250
STESSEATVK KKPAPKTPPK AATPPQITQF PEDRKVRAGE SVELFAKVVG
1260 1270 1280 1290 1300
TAPITCTWMK FRKQIQENEY IKIENAENSS KLTISSTKQE HCGCYTLVVE
1310 1320 1330 1340 1350
NKLGSRQAQV NLTVVDKPDP PAGTPCASDI RSSSLTLSWY GSSYDGGSAV
1360 1370 1380 1390 1400
QSYTVEIWNS VDNKWTDLTT CRSTSFNVQD LQADREYKFR VRAANVYGIS
1410 1420 1430 1440 1450
EPSQESEVVK VGEKQEEELK EEEAELSDDE GKETEVNYRT VTINTEQKVS
1460 1470 1480 1490 1500
DVYNIEERLG SGKFGQVFRL VEKKTGKVWA GKFFKAYSAK EKENIRDEIS
1510 1520 1530 1540 1550
IMNCLHHPKL VQCVDAFEEK ANIVMVLEMV SGGELFERII DEDFELTERE
1560 1570 1580 1590 1600
CIKYMRQISE GVEYIHKQGI VHLDLKPENI MCVNKTGTSI KLIDFGLARR
1610 1620 1630 1640 1650
LESAGSLKVL FGTPEFVAPE VINYEPIGYE TDMWSIGVIC YILVSGLSPF
1660 1670 1680 1690 1700
MGDNDNETLA NVTSATWDFD DEAFDEISDD AKDFISNLLK KDMKSRLNCT
1710 1720 1730 1740 1750
QCLQHPWLQK DTKNMEAKKL SKDRMKKYMA RRKWQKTGHA VRAIGRLSSM
1760 1770 1780 1790 1800
AMISGMSGRK ASGSSPTSPI NADKVENEDA FLEEVAEEKP HVKPYFTKTI
1810 1820 1830 1840 1850
LDMEVVEGSA ARFDCKIEGY PDPEVMWYKD DQPVKESRHF QIDYDEEGNC
1860 1870 1880 1890 1900
SLTISEVCGD DDAKYTCKAV NSLGEATCTA ELLVETMGKE GEGEGEGEED

EEEEEE
Length:1,906
Mass (Da):210,446
Last modified:July 15, 1998 - v2
Checksum:iAD7D8A3B69EE3363
GO
Isoform 2 (identifier: P11799-2) [UniParc]FASTAAdd to basket
Also known as: MLCK-210, Non-muscle

The sequence of this isoform differs from the canonical sequence as follows:
     1-934: Missing.

Show »
Length:972
Mass (Da):107,547
Checksum:i375ED3627731EFFE
GO
Isoform 3 (identifier: P11799-3) [UniParc]FASTAAdd to basket
Also known as: Telokin

The sequence of this isoform differs from the canonical sequence as follows:
     1-1749: Missing.

Show »
Length:157
Mass (Da):17,337
Checksum:i557E7CD96C672E7D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1439R → Q in AAA49069 (PubMed:3030394).Curated1
Sequence conflicti1439R → Q in AAA69964 (PubMed:3030394).Curated1
Sequence conflicti1776E → D AA sequence (PubMed:9283094).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188521 – 1749Missing in isoform 3. 2 PublicationsAdd BLAST1749
Alternative sequenceiVSP_0188511 – 934Missing in isoform 2. 1 PublicationAdd BLAST934

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52876 mRNA. Translation: CAA37056.1.
X52876 mRNA. Translation: CAA37057.1.
X52876 mRNA. Translation: CAA37058.1.
M31048 mRNA. Translation: AAA49069.1.
M14953 mRNA. Translation: AAA69964.1.
AF045285
, AF045255, AF045256, AF045257, AF045260, AF045259, AF045258, AF045261, AF045263, AF045265, AF045274, AF045273, AF045272, AF045271, AF045270, AF045269, AF045268, AF045267, AF045266, AF045283, AF045282, AF045281, AF045280, AF045279, AF045278, AF045277, AF045276, AF045275, AF045284, AF045264, AF045262 Genomic DNA. Translation: AAC29031.1.
M96655 mRNA. Translation: AAA49083.1.
M88283 mRNA. Translation: AAA48647.1.
M88284 Genomic DNA. Translation: AAB53768.1.
PIRiS68235.
RefSeqiXP_015145280.1. XM_015289794.1. [P11799-3]
UniGeneiGga.4091.

Genome annotation databases

GeneIDi396445.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52876 mRNA. Translation: CAA37056.1.
X52876 mRNA. Translation: CAA37057.1.
X52876 mRNA. Translation: CAA37058.1.
M31048 mRNA. Translation: AAA49069.1.
M14953 mRNA. Translation: AAA69964.1.
AF045285
, AF045255, AF045256, AF045257, AF045260, AF045259, AF045258, AF045261, AF045263, AF045265, AF045274, AF045273, AF045272, AF045271, AF045270, AF045269, AF045268, AF045267, AF045266, AF045283, AF045282, AF045281, AF045280, AF045279, AF045278, AF045277, AF045276, AF045275, AF045284, AF045264, AF045262 Genomic DNA. Translation: AAC29031.1.
M96655 mRNA. Translation: AAA49083.1.
M88283 mRNA. Translation: AAA48647.1.
M88284 Genomic DNA. Translation: AAB53768.1.
PIRiS68235.
RefSeqiXP_015145280.1. XM_015289794.1. [P11799-3]
UniGeneiGga.4091.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CDLX-ray2.00E/F/G/H1730-1749[»]
1QS7X-ray1.80B/D1731-1749[»]
1QTXX-ray1.65B1731-1749[»]
1VRKX-ray1.90B1731-1749[»]
2O5GX-ray1.08B1730-1748[»]
3EVUX-ray1.75A1731-1749[»]
3EVVX-ray2.60A1731-1749[»]
3O77X-ray2.35A1730-1749[»]
3O78X-ray2.60A/B1731-1749[»]
ProteinModelPortaliP11799.
SMRiP11799.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi676694. 1 interactor.
IntActiP11799. 3 interactors.
STRINGi9031.ENSGALP00000019112.

Chemistry databases

BindingDBiP11799.
ChEMBLiCHEMBL3062.

PTM databases

iPTMnetiP11799.

Proteomic databases

PaxDbiP11799.
PRIDEiP11799.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396445.

Organism-specific databases

CTDi4638.

Phylogenomic databases

eggNOGiKOG0613. Eukaryota.
ENOG410XQFD. LUCA.
HOGENOMiHOG000049287.
HOVERGENiHBG052551.
PhylomeDBiP11799.

Enzyme and pathway databases

BRENDAi2.7.11.18. 1306.

Miscellaneous databases

EvolutionaryTraceiP11799.
PROiP11799.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 10 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR015725. Telokin/Myosin_light_ch_kin.
[Graphical view]
PANTHERiPTHR10489:SF700. PTHR10489:SF700. 4 hits.
PfamiPF00041. fn3. 1 hit.
PF07679. I-set. 9 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
SM00409. IG. 9 hits.
SM00408. IGc2. 9 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 9 hits.
SSF49265. SSF49265. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50853. FN3. 1 hit.
PS50835. IG_LIKE. 9 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYLK_CHICK
AccessioniPrimary (citable) accession number: P11799
Secondary accession number(s): P19038, Q549S2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 15, 1998
Last modified: November 30, 2016
This is version 175 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.