P11747 (TAF13_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription initiation factor TFIID subunit 13

Alternative name(s):
Function unknown 81 protein
TAFII-19
Short name=TAFII19
TBP-associated factor 13
TBP-associated factor 19 kDa

Gene names

Name:	TAF13
Synonyms:	FUN81, TAF19
Ordered Locus Names:	YML098W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
Subunit structure	In TFIID, TAF13 heterodimerizes with TAF11, but they do not seem to form a heterotetramer like TAF6/TAF9. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14. Ref.6
Subcellular location	Nucleus Ref.10.
Miscellaneous	Present with 2100 molecules/cell in log phase SD medium. Ref.11
Sequence similarities	Belongs to the TAF13 family. Contains 1 histone-fold domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA polymerase II transcriptional preinitiation complex assembly Inferred from mutant phenotype. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	transcription factor TFIID complex Inferred from direct assay Ref.6. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TAF11	Q04226	5	EBI-18897,EBI-18884

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 167	167	Transcription initiation factor TFIID subunit 13		PRO_0000118912
Regions
	Domain	10 – 74	65	Histone-fold
	Coiled coil	109 – 129	21	Potential
	Compositional bias	113 – 136	24	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	131	1	Phosphothreonine Ref.13
Experimental info
	Sequence conflict	137 – 138	2	AA → GV in AAA34610. Ref.1
	Sequence conflict	158	1	G → A in AAA34610. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P11747 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: F54E639C0A59C506	FASTA	167	19,132
	10 20 30 40 50 60 MSRKLKKTNL FNKDVSSLLY AYGDVPQPLQ ATVQCLDELV SGYLVDVCTN AFHTAQNSQR 70 80 90 100 110 120 NKLRLEDFKF ALRKDPIKLG RAEELIATNK LITEAKKQFN ETDNQNSLKR YREEDEEGDE 130 140 150 160 MEEDEDEQQV TDDDEEAAGR NSAKQSTDSK ATKIRKQGPK NLKKTKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of two new genes essential for vegetative growth in Saccharomyces cerevisiae: nucleotide sequence determination and chromosome mapping." Dubois E., Bercy J., Descamps F., Messenguy F. Gene 55:265-275(1987) [PubMed: 3311883] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Human TAF(II)28 and TAF(II)18 interact through a histone fold encoded by atypical evolutionary conserved motifs also found in the SPT3 family." Birck C., Poch O., Romier C., Ruff M., Mengus G., Lavigne A.C., Davidson I., Moras D. Cell 94:239-249(1998) [PubMed: 9695952] [Abstract] Cited for: FUNCTION, TAF-TAF INTERACTION THROUGH HISTONE-FOLD DOMAIN.
[6]	"Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex." Sanders S.L., Weil P.A. J. Biol. Chem. 275:13895-13900(2000) [PubMed: 10788514] [Abstract] Cited for: FUNCTION, SUBUNIT.
[7]	"The histone fold is a key structural motif of transcription factor TFIID." Gangloff Y.G., Romier C., Thuault S., Werten S., Davidson I. Trends Biochem. Sci. 26:250-257(2001) [PubMed: 11295558] [Abstract] Cited for: FUNCTION, HISTONE-FOLD DOMAIN CHARACTERIZATION.
[8]	"Molecular characterization of Saccharomyces cerevisiae TFIID." Sanders S.L., Garbett K.A., Weil P.A. Mol. Cell. Biol. 22:6000-6013(2002) [PubMed: 12138208] [Abstract] Cited for: FUNCTION, TFIID STOICHIOMETRY.
[9]	"Multi-protein complexes in eukaryotic gene transcription." Martinez E. Plant Mol. Biol. 50:925-947(2002) [PubMed: 12516863] [Abstract] Cited for: FUNCTION.
[10]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]	"Mapping histone fold TAFs within yeast TFIID." Leurent C., Sanders S.L., Ruhlmann C., Mallouh V., Weil P.A., Kirschner D.B., Tora L., Schultz P. EMBO J. 21:3424-3433(2002) [PubMed: 12093743] [Abstract] Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.
[13]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-131, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M17512 Genomic DNA. Translation: AAA34610.1. Z46660 Genomic DNA. Translation: CAA86639.1. AY557763 Genomic DNA. Translation: AAS56089.1. BK006946 Genomic DNA. Translation: DAA09801.1.
PIR	S49628.
RefSeq	NP_013611.1. NM_001182458.1.
3D structure databases
ProteinModelPortal	P11747.
SMR	P11747. Positions 10-54.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2054N.
IntAct	P11747. 25 interactions.
MINT	MINT-490157.
STRING	P11747.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML098W; YML098W; YML098W.
GeneID	854875.
KEGG	sce:YML098W.
NMPDR	fig|4932.3.peg.4648.
Organism-specific databases
CYGD	YML098w.
SGD	S000004564. TAF13.
Phylogenomic databases
eggNOG	fuNOG12211.
GeneTree	EFGT00050000004406.
OMA	DICANAY.
OrthoDB	EOG42Z80V.
Gene expression databases
ArrayExpress	P11747.
Genevestigator	P11747.
GermOnline	YML098W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009072. Histone-fold. IPR003195. TFIID-18. [Graphical view]
Gene3D	G3DSA:1.10.20.10. Histone-fold. 1 hit.
KO	K03127.
PANTHER	PTHR11380. TFIID-18. 1 hit.
Pfam	PF02269. TFIID-18kDa. 1 hit. [Graphical view]
SUPFAM	SSF47113. Histone-fold. 1 hit.
ProtoNet	Search...
Other
NextBio	977813.

Entry information

Entry name

TAF13_YEAST

Accession

Primary (citable) accession number: P11747
Secondary accession number(s): D6W0I7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program