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P11746

- MCM1_YEAST

UniProt

P11746 - MCM1_YEAST

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Protein

Pheromone receptor transcription factor

Gene

MCM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcription factor required for the efficient replication of minichromosomes and the transcriptional regulation of early cell cycle genes. Activates transcription of ECB-dependent genes during the G1/M phase. Genes that contain a ECB (early cell box) element in their transcription regulatory region are transcribed only during G1/M phases. Interacts with the alpha-2 repressor or with the alpha-1 activator thereby regulating the expression of mating-type-specific genes. With ARG80, ARG81 and ARG82, coordinates the expression of arginine anabolic and catabolic genes in response to arginine.

GO - Molecular functioni

  1. DNA replication origin binding Source: SGD
  2. RNA polymerase II activating transcription factor binding Source: SGD
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
  4. RNA polymerase II core promoter proximal region sequence-specific DNA binding, bending Source: SGD
  5. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: SGD
  6. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD
  7. RNA polymerase II repressing transcription factor binding Source: SGD
  8. sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity Source: SGD

GO - Biological processi

  1. arginine metabolic process Source: UniProtKB-KW
  2. DNA replication initiation Source: SGD
  3. negative regulation of arginine catabolic process by negative regulation of transcription from RNA polymerase II promoter Source: SGD
  4. negative regulation of mating-type specific transcription from RNA polymerase II promoter Source: SGD
  5. negative regulation of transcription from RNA polymerase II promoter Source: SGD
  6. positive regulation of arginine biosynthetic process by positive regulation of transcription from RNA polymerase II promoter Source: SGD
  7. positive regulation of mating-type specific transcription from RNA polymerase II promoter Source: SGD
  8. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  9. positive regulation of transcription involved in G2/M transition of mitotic cell cycle Source: SGD
  10. regulation of mating type switching Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Arginine metabolism, Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32748-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pheromone receptor transcription factor
Alternative name(s):
GRM/PRTF protein
Gene namesi
Name:MCM1
Synonyms:FUN80
Ordered Locus Names:YMR043W
ORF Names:YM9532.08
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR043w.
SGDiS000004646. MCM1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
  2. nuclear chromatin Source: SGD
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 286285Pheromone receptor transcription factorPRO_0000199439Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei2 – 21Phosphoserine1 Publication
Modified residuei144 – 1441Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP11746.

Expressioni

Gene expression databases

GenevestigatoriP11746.

Interactioni

Subunit structurei

Homodimer. Binds DNA with a high specificity in complex with mating-type protein ALPHA1. Also binds DNA with a high specificity as a heterotetramer consisting of an ALPHA2 dimer and an MCM1 dimer. Interacts with YHP1 and YOX1, possibly leading to its inactivation. Interacts with ARG80 and ARG82.5 Publications

Protein-protein interaction databases

BioGridi35216. 39 interactions.
DIPiDIP-72N.
IntActiP11746. 51 interactions.
MINTiMINT-478633.
STRINGi4932.YMR043W.

Structurei

Secondary structure

1
286
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 5426
Beta strandi57 – 637
Beta strandi69 – 735
Turni75 – 784
Helixi79 – 824
Helixi84 – 9411

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MNMX-ray2.25A/B1-100[»]
ProteinModelPortaliP11746.
SMRiP11746. Positions 18-98.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11746.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 7255MADS-boxPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi98 – 12023Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 1 MADS-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5068.
GeneTreeiENSGT00400000022158.
InParanoidiP11746.
KOiK12412.
OMAiCLNAPDD.
OrthoDBiEOG7M0P1S.

Family and domain databases

InterProiIPR002100. TF_MADSbox.
[Graphical view]
PfamiPF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11746-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDIEEGTPT NNGQQKERRK IEIKFIENKT RRHVTFSKRK HGIMKKAFEL
60 70 80 90 100
SVLTGTQVLL LVVSETGLVY TFSTPKFEPI VTQQEGRNLI QACLNAPDDE
110 120 130 140 150
EEDEEEDGDD DDDDDDDGND MQRQQPQQQQ PQQQQQVLNA HANSLGHLNQ
160 170 180 190 200
DQVPAGALKQ EVKSQLLGGA NPNQNSMIQQ QQHHTQNSQP QQQQQQQPQQ
210 220 230 240 250
QMSQQQMSQH PRPQQGIPHP QQSQPQQQQQ QQQQLQQQQQ QQQQQPLTGI
260 270 280
HQPHQQAFAN AASPYLNAEQ NAAYQQYFQE PQQGQY
Length:286
Mass (Da):32,802
Last modified:February 1, 1991 - v2
Checksum:iFD75B4BF549E9E3B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91P → S in AAA34609. (PubMed:3311883)Curated
Sequence conflicti37 – 371S → F in AAA34609. (PubMed:3311883)Curated
Sequence conflicti156 – 1572GA → AR in AAA34609. (PubMed:3311883)Curated
Sequence conflicti158 – 286129Missing in AAA34609. (PubMed:3311883)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52453 Genomic DNA. Translation: CAA36691.1.
X14187 Genomic DNA. Translation: CAA32389.1.
M17511 Genomic DNA. Translation: AAA34609.1.
Z48502 Genomic DNA. Translation: CAA88409.1.
AY557985 Genomic DNA. Translation: AAS56311.1.
BK006946 Genomic DNA. Translation: DAA09942.1.
PIRiA34599.
RefSeqiNP_013757.1. NM_001182540.1.

Genome annotation databases

EnsemblFungiiYMR043W; YMR043W; YMR043W.
GeneIDi855060.
KEGGisce:YMR043W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52453 Genomic DNA. Translation: CAA36691.1 .
X14187 Genomic DNA. Translation: CAA32389.1 .
M17511 Genomic DNA. Translation: AAA34609.1 .
Z48502 Genomic DNA. Translation: CAA88409.1 .
AY557985 Genomic DNA. Translation: AAS56311.1 .
BK006946 Genomic DNA. Translation: DAA09942.1 .
PIRi A34599.
RefSeqi NP_013757.1. NM_001182540.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MNM X-ray 2.25 A/B 1-100 [» ]
ProteinModelPortali P11746.
SMRi P11746. Positions 18-98.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35216. 39 interactions.
DIPi DIP-72N.
IntActi P11746. 51 interactions.
MINTi MINT-478633.
STRINGi 4932.YMR043W.

Proteomic databases

PaxDbi P11746.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR043W ; YMR043W ; YMR043W .
GeneIDi 855060.
KEGGi sce:YMR043W.

Organism-specific databases

CYGDi YMR043w.
SGDi S000004646. MCM1.

Phylogenomic databases

eggNOGi COG5068.
GeneTreei ENSGT00400000022158.
InParanoidi P11746.
KOi K12412.
OMAi CLNAPDD.
OrthoDBi EOG7M0P1S.

Enzyme and pathway databases

BioCyci YEAST:G3O-32748-MONOMER.

Miscellaneous databases

EvolutionaryTracei P11746.
NextBioi 978315.
PROi P11746.

Gene expression databases

Genevestigatori P11746.

Family and domain databases

InterProi IPR002100. TF_MADSbox.
[Graphical view ]
Pfami PF00319. SRF-TF. 1 hit.
[Graphical view ]
PRINTSi PR00404. MADSDOMAIN.
SMARTi SM00432. MADS. 1 hit.
[Graphical view ]
SUPFAMi SSF55455. SSF55455. 1 hit.
PROSITEi PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification, purification, and cloning of a polypeptide (PRTF/GRM) that binds to mating-specific promoter elements in yeast."
    Ammerer G.
    Genes Dev. 4:299-312(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Saccharomyces cerevisiae protein involved in plasmid maintenance is necessary for mating of MAT alpha cells."
    Passmore S., Maine G.T., Elble R., Christ C., Tye B.K.
    J. Mol. Biol. 204:593-606(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Characterization of two new genes essential for vegetative growth in Saccharomyces cerevisiae: nucleotide sequence determination and chromosome mapping."
    Dubois E., Bercy J., Descamps F., Messenguy F.
    Gene 55:265-275(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. "The DNA binding and oligomerization domain of MCM1 is sufficient for its interaction with other regulatory proteins."
    Primig M., Winkler H., Ammerer G.
    EMBO J. 10:4209-4218(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ALPHA1 AND ALPHA2.
  8. "The yeast alpha2 and Mcm1 proteins interact through a region similar to a motif found in homeodomain proteins of higher eukaryotes."
    Mead J., Zhong H., Acton T.B., Vershon A.K.
    Mol. Cell. Biol. 16:2135-2143(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ALPHA2.
  9. "Recruitment of the yeast MADS-box proteins, ArgRI and Mcm1 by the pleiotropic factor ArgRIII is required for their stability."
    El Bakkoury M., Dubois E., Messenguy F.
    Mol. Microbiol. 35:15-31(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARG81 AND ARG82.
  10. "Conserved homeodomain proteins interact with MADS box protein Mcm1 to restrict ECB-dependent transcription to the M/G1 phase of the cell cycle."
    Pramila T., Miles S., GuhaThakurta D., Jemiolo D., Breeden L.L.
    Genes Dev. 16:3034-3045(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YHP1 AND YOX1.
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-144, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  15. "Crystal structure of the yeast MATalpha2/MCM1/DNA ternary complex."
    Tan S., Richmond T.J.
    Nature 391:660-666(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 1-100 IN COMPLEX WITH ALPHA2.

Entry informationi

Entry nameiMCM1_YEAST
AccessioniPrimary (citable) accession number: P11746
Secondary accession number(s): D6VZL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: February 1, 1991
Last modified: October 29, 2014
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3