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Protein

Pheromone receptor transcription factor

Gene

MCM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor required for the efficient replication of minichromosomes and the transcriptional regulation of early cell cycle genes. Activates transcription of ECB-dependent genes during the G1/M phase. Genes that contain a ECB (early cell box) element in their transcription regulatory region are transcribed only during G1/M phases. Interacts with the alpha-2 repressor or with the alpha-1 activator thereby regulating the expression of mating-type-specific genes. With ARG80, ARG81 and ARG82, coordinates the expression of arginine anabolic and catabolic genes in response to arginine.

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • arginine metabolic process Source: UniProtKB-KW
  • DNA replication initiation Source: SGD
  • negative regulation of arginine catabolic process by negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • negative regulation of mating-type specific transcription from RNA polymerase II promoter Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of arginine biosynthetic process by positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of mating-type specific transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription involved in G2/M transition of mitotic cell cycle Source: SGD
  • regulation of mating type switching Source: SGD

Keywordsi

Molecular functionActivator, DNA-binding
Biological processArginine metabolism, Cell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32748-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pheromone receptor transcription factor
Alternative name(s):
GRM/PRTF protein
Gene namesi
Name:MCM1
Synonyms:FUN80
Ordered Locus Names:YMR043W
ORF Names:YM9532.08
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR043W.
SGDiS000004646. MCM1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001994392 – 286Pheromone receptor transcription factorAdd BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineCombined sources1
Modified residuei144PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP11746.

PTM databases

iPTMnetiP11746.

Interactioni

Subunit structurei

Homodimer. Binds DNA with a high specificity in complex with mating-type protein ALPHA1. Also binds DNA with a high specificity as a heterotetramer consisting of an ALPHA2 dimer and an MCM1 dimer. Interacts with YHP1 and YOX1, possibly leading to its inactivation. Interacts with ARG80 and ARG82.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HHF2P023093EBI-10528,EBI-8113

GO - Molecular functioni

  • protein dimerization activity Source: InterPro
  • RNA polymerase II activating transcription factor binding Source: SGD
  • RNA polymerase II repressing transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi35216. 398 interactors.
DIPiDIP-72N.
IntActiP11746. 59 interactors.
MINTiMINT-478633.
STRINGi4932.YMR043W.

Structurei

Secondary structure

1286
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi29 – 54Combined sources26
Beta strandi57 – 63Combined sources7
Beta strandi69 – 73Combined sources5
Turni75 – 78Combined sources4
Helixi79 – 82Combined sources4
Helixi84 – 94Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MNMX-ray2.25A/B1-100[»]
ProteinModelPortaliP11746.
SMRiP11746.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11746.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 72MADS-boxPROSITE-ProRule annotationAdd BLAST55

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi98 – 120Asp/Glu-rich (acidic)Add BLAST23

Phylogenomic databases

GeneTreeiENSGT00400000022158.
InParanoidiP11746.
KOiK12412.
OMAiVLNAHAN.
OrthoDBiEOG092C1PUJ.

Family and domain databases

CDDicd00266. MADS_SRF_like. 1 hit.
Gene3Di3.40.1810.10. 1 hit.
InterProiView protein in InterPro
IPR033897. MADS_SRF-like.
IPR002100. TF_MADSbox.
PfamiView protein in Pfam
PF00319. SRF-TF. 1 hit.
PRINTSiPR00404. MADSDOMAIN.
SMARTiView protein in SMART
SM00432. MADS. 1 hit.
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiView protein in PROSITE
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11746-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDIEEGTPT NNGQQKERRK IEIKFIENKT RRHVTFSKRK HGIMKKAFEL
60 70 80 90 100
SVLTGTQVLL LVVSETGLVY TFSTPKFEPI VTQQEGRNLI QACLNAPDDE
110 120 130 140 150
EEDEEEDGDD DDDDDDDGND MQRQQPQQQQ PQQQQQVLNA HANSLGHLNQ
160 170 180 190 200
DQVPAGALKQ EVKSQLLGGA NPNQNSMIQQ QQHHTQNSQP QQQQQQQPQQ
210 220 230 240 250
QMSQQQMSQH PRPQQGIPHP QQSQPQQQQQ QQQQLQQQQQ QQQQQPLTGI
260 270 280
HQPHQQAFAN AASPYLNAEQ NAAYQQYFQE PQQGQY
Length:286
Mass (Da):32,802
Last modified:February 1, 1991 - v2
Checksum:iFD75B4BF549E9E3B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9P → S in AAA34609 (PubMed:3311883).Curated1
Sequence conflicti37S → F in AAA34609 (PubMed:3311883).Curated1
Sequence conflicti156 – 157GA → AR in AAA34609 (PubMed:3311883).Curated2
Sequence conflicti158 – 286Missing in AAA34609 (PubMed:3311883).CuratedAdd BLAST129

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52453 Genomic DNA. Translation: CAA36691.1.
X14187 Genomic DNA. Translation: CAA32389.1.
M17511 Genomic DNA. Translation: AAA34609.1.
Z48502 Genomic DNA. Translation: CAA88409.1.
AY557985 Genomic DNA. Translation: AAS56311.1.
BK006946 Genomic DNA. Translation: DAA09942.1.
PIRiA34599.
RefSeqiNP_013757.1. NM_001182540.1.

Genome annotation databases

EnsemblFungiiYMR043W; YMR043W; YMR043W.
GeneIDi855060.
KEGGisce:YMR043W.

Similar proteinsi

Entry informationi

Entry nameiMCM1_YEAST
AccessioniPrimary (citable) accession number: P11746
Secondary accession number(s): D6VZL8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: February 1, 1991
Last modified: September 27, 2017
This is version 169 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names