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P11746

- MCM1_YEAST

UniProt

P11746 - MCM1_YEAST

Protein

Pheromone receptor transcription factor

Gene

MCM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 145 (01 Oct 2014)
      Sequence version 2 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Transcription factor required for the efficient replication of minichromosomes and the transcriptional regulation of early cell cycle genes. Activates transcription of ECB-dependent genes during the G1/M phase. Genes that contain a ECB (early cell box) element in their transcription regulatory region are transcribed only during G1/M phases. Interacts with the alpha-2 repressor or with the alpha-1 activator thereby regulating the expression of mating-type-specific genes. With ARG80, ARG81 and ARG82, coordinates the expression of arginine anabolic and catabolic genes in response to arginine.

    GO - Molecular functioni

    1. DNA replication origin binding Source: SGD
    2. RNA polymerase II activating transcription factor binding Source: SGD
    3. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
    4. RNA polymerase II core promoter proximal region sequence-specific DNA binding, bending Source: SGD
    5. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: SGD
    6. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD
    7. RNA polymerase II repressing transcription factor binding Source: SGD
    8. sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity Source: SGD

    GO - Biological processi

    1. arginine metabolic process Source: UniProtKB-KW
    2. DNA replication initiation Source: SGD
    3. negative regulation of arginine catabolic process by negative regulation of transcription from RNA polymerase II promoter Source: SGD
    4. negative regulation of mating-type specific transcription from RNA polymerase II promoter Source: SGD
    5. negative regulation of transcription from RNA polymerase II promoter Source: SGD
    6. positive regulation of arginine biosynthetic process by positive regulation of transcription from RNA polymerase II promoter Source: SGD
    7. positive regulation of mating-type specific transcription from RNA polymerase II promoter Source: SGD
    8. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    9. positive regulation of transcription involved in G2/M transition of mitotic cell cycle Source: SGD
    10. regulation of mating type switching Source: SGD

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Arginine metabolism, Cell cycle, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32748-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pheromone receptor transcription factor
    Alternative name(s):
    GRM/PRTF protein
    Gene namesi
    Name:MCM1
    Synonyms:FUN80
    Ordered Locus Names:YMR043W
    ORF Names:YM9532.08
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR043w.
    SGDiS000004646. MCM1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. nuclear chromatin Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 286285Pheromone receptor transcription factorPRO_0000199439Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei2 – 21Phosphoserine1 Publication
    Modified residuei144 – 1441Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP11746.

    Expressioni

    Gene expression databases

    GenevestigatoriP11746.

    Interactioni

    Subunit structurei

    Homodimer. Binds DNA with a high specificity in complex with mating-type protein ALPHA1. Also binds DNA with a high specificity as a heterotetramer consisting of an ALPHA2 dimer and an MCM1 dimer. Interacts with YHP1 and YOX1, possibly leading to its inactivation. Interacts with ARG80 and ARG82.5 Publications

    Protein-protein interaction databases

    BioGridi35216. 39 interactions.
    DIPiDIP-72N.
    IntActiP11746. 51 interactions.
    MINTiMINT-478633.
    STRINGi4932.YMR043W.

    Structurei

    Secondary structure

    1
    286
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi29 – 5426
    Beta strandi57 – 637
    Beta strandi69 – 735
    Turni75 – 784
    Helixi79 – 824
    Helixi84 – 9411

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1MNMX-ray2.25A/B1-100[»]
    ProteinModelPortaliP11746.
    SMRiP11746. Positions 18-98.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP11746.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini18 – 7255MADS-boxPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi98 – 12023Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Contains 1 MADS-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5068.
    GeneTreeiENSGT00400000022158.
    KOiK12412.
    OMAiCLNAPDD.
    OrthoDBiEOG7M0P1S.

    Family and domain databases

    InterProiIPR002100. TF_MADSbox.
    [Graphical view]
    PfamiPF00319. SRF-TF. 1 hit.
    [Graphical view]
    PRINTSiPR00404. MADSDOMAIN.
    SMARTiSM00432. MADS. 1 hit.
    [Graphical view]
    SUPFAMiSSF55455. SSF55455. 1 hit.
    PROSITEiPS00350. MADS_BOX_1. 1 hit.
    PS50066. MADS_BOX_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P11746-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDIEEGTPT NNGQQKERRK IEIKFIENKT RRHVTFSKRK HGIMKKAFEL    50
    SVLTGTQVLL LVVSETGLVY TFSTPKFEPI VTQQEGRNLI QACLNAPDDE 100
    EEDEEEDGDD DDDDDDDGND MQRQQPQQQQ PQQQQQVLNA HANSLGHLNQ 150
    DQVPAGALKQ EVKSQLLGGA NPNQNSMIQQ QQHHTQNSQP QQQQQQQPQQ 200
    QMSQQQMSQH PRPQQGIPHP QQSQPQQQQQ QQQQLQQQQQ QQQQQPLTGI 250
    HQPHQQAFAN AASPYLNAEQ NAAYQQYFQE PQQGQY 286
    Length:286
    Mass (Da):32,802
    Last modified:February 1, 1991 - v2
    Checksum:iFD75B4BF549E9E3B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti9 – 91P → S in AAA34609. (PubMed:3311883)Curated
    Sequence conflicti37 – 371S → F in AAA34609. (PubMed:3311883)Curated
    Sequence conflicti156 – 1572GA → AR in AAA34609. (PubMed:3311883)Curated
    Sequence conflicti158 – 286129Missing in AAA34609. (PubMed:3311883)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52453 Genomic DNA. Translation: CAA36691.1.
    X14187 Genomic DNA. Translation: CAA32389.1.
    M17511 Genomic DNA. Translation: AAA34609.1.
    Z48502 Genomic DNA. Translation: CAA88409.1.
    AY557985 Genomic DNA. Translation: AAS56311.1.
    BK006946 Genomic DNA. Translation: DAA09942.1.
    PIRiA34599.
    RefSeqiNP_013757.1. NM_001182540.1.

    Genome annotation databases

    EnsemblFungiiYMR043W; YMR043W; YMR043W.
    GeneIDi855060.
    KEGGisce:YMR043W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52453 Genomic DNA. Translation: CAA36691.1 .
    X14187 Genomic DNA. Translation: CAA32389.1 .
    M17511 Genomic DNA. Translation: AAA34609.1 .
    Z48502 Genomic DNA. Translation: CAA88409.1 .
    AY557985 Genomic DNA. Translation: AAS56311.1 .
    BK006946 Genomic DNA. Translation: DAA09942.1 .
    PIRi A34599.
    RefSeqi NP_013757.1. NM_001182540.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1MNM X-ray 2.25 A/B 1-100 [» ]
    ProteinModelPortali P11746.
    SMRi P11746. Positions 18-98.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35216. 39 interactions.
    DIPi DIP-72N.
    IntActi P11746. 51 interactions.
    MINTi MINT-478633.
    STRINGi 4932.YMR043W.

    Proteomic databases

    PaxDbi P11746.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR043W ; YMR043W ; YMR043W .
    GeneIDi 855060.
    KEGGi sce:YMR043W.

    Organism-specific databases

    CYGDi YMR043w.
    SGDi S000004646. MCM1.

    Phylogenomic databases

    eggNOGi COG5068.
    GeneTreei ENSGT00400000022158.
    KOi K12412.
    OMAi CLNAPDD.
    OrthoDBi EOG7M0P1S.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32748-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P11746.
    NextBioi 978315.
    PROi P11746.

    Gene expression databases

    Genevestigatori P11746.

    Family and domain databases

    InterProi IPR002100. TF_MADSbox.
    [Graphical view ]
    Pfami PF00319. SRF-TF. 1 hit.
    [Graphical view ]
    PRINTSi PR00404. MADSDOMAIN.
    SMARTi SM00432. MADS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55455. SSF55455. 1 hit.
    PROSITEi PS00350. MADS_BOX_1. 1 hit.
    PS50066. MADS_BOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification, purification, and cloning of a polypeptide (PRTF/GRM) that binds to mating-specific promoter elements in yeast."
      Ammerer G.
      Genes Dev. 4:299-312(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Saccharomyces cerevisiae protein involved in plasmid maintenance is necessary for mating of MAT alpha cells."
      Passmore S., Maine G.T., Elble R., Christ C., Tye B.K.
      J. Mol. Biol. 204:593-606(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Characterization of two new genes essential for vegetative growth in Saccharomyces cerevisiae: nucleotide sequence determination and chromosome mapping."
      Dubois E., Bercy J., Descamps F., Messenguy F.
      Gene 55:265-275(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    7. "The DNA binding and oligomerization domain of MCM1 is sufficient for its interaction with other regulatory proteins."
      Primig M., Winkler H., Ammerer G.
      EMBO J. 10:4209-4218(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ALPHA1 AND ALPHA2.
    8. "The yeast alpha2 and Mcm1 proteins interact through a region similar to a motif found in homeodomain proteins of higher eukaryotes."
      Mead J., Zhong H., Acton T.B., Vershon A.K.
      Mol. Cell. Biol. 16:2135-2143(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ALPHA2.
    9. "Recruitment of the yeast MADS-box proteins, ArgRI and Mcm1 by the pleiotropic factor ArgRIII is required for their stability."
      El Bakkoury M., Dubois E., Messenguy F.
      Mol. Microbiol. 35:15-31(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ARG81 AND ARG82.
    10. "Conserved homeodomain proteins interact with MADS box protein Mcm1 to restrict ECB-dependent transcription to the M/G1 phase of the cell cycle."
      Pramila T., Miles S., GuhaThakurta D., Jemiolo D., Breeden L.L.
      Genes Dev. 16:3034-3045(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YHP1 AND YOX1.
    11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-144, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    15. "Crystal structure of the yeast MATalpha2/MCM1/DNA ternary complex."
      Tan S., Richmond T.J.
      Nature 391:660-666(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 1-100 IN COMPLEX WITH ALPHA2.

    Entry informationi

    Entry nameiMCM1_YEAST
    AccessioniPrimary (citable) accession number: P11746
    Secondary accession number(s): D6VZL8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 145 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 8970 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3