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P11745 (RNA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 144. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ran GTPase-activating protein 1
Alternative name(s):
Protein involved in RNA production/processing
Gene names
Name:RNA1
Ordered Locus Names:YMR235C
ORF Names:YM9959.17C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length407 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase activator for the nuclear Ras-related regulatory protein GSP1 (Ran), converting it to the putatively inactive GDP-bound state.

Subcellular location

Cytoplasm.

Disruption phenotype

Causes conditional lethality. Strains bearing this mutation do not grow at temperatures exceeding 30 degrees Celsius. Ref.1

Miscellaneous

Present with 52200 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the RNA1 family.

Contains 11 LRR (leucine-rich) repeats.

Sequence caution

The sequence CAA35248.1 differs from that shown. Reason: Frameshift at positions 185 and 203.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 407407Ran GTPase-activating protein 1
PRO_0000056742

Regions

Repeat11 – 3929LRR 1
Repeat40 – 6728LRR 2
Repeat68 – 10134LRR 3
Repeat102 – 13332LRR 4
Repeat134 – 16633LRR 5
Repeat167 – 19731LRR 6
Repeat198 – 22629LRR 7
Repeat227 – 25630LRR 8
Repeat257 – 28529LRR 9
Repeat286 – 31530LRR 10
Repeat316 – 34631LRR 11
Compositional bias348 – 37528Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue3601Phosphoserine Ref.8 Ref.9

Natural variations

Natural variant171S → F in mutant RNA1-1.
Natural variant1941A → V in mutant RNA1-1.

Experimental info

Sequence conflict421K → E in CAA35248. Ref.2
Sequence conflict2221L → S in CAA40449. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P11745 [UniParc].

Last modified April 1, 1990. Version 2.
Checksum: D77029B6783FFF09

FASTA40745,816
        10         20         30         40         50         60 
MATLHFVPQH EEEQVYSISG KALKLTTSDD IKPYLEELAA LKTCTKLDLS GNTIGTEASE 

        70         80         90        100        110        120 
ALAKCIAENT QVRESLVEVN FADLYTSRLV DEVVDSLKFL LPVLLKCPHL EIVNLSDNAF 

       130        140        150        160        170        180 
GLRTIELLED YIAHAVNIKH LILSNNGMGP FAGERIGKAL FHLAQNKKAA SKPFLETFIC 

       190        200        210        220        230        240 
GRNRLENGSA VYLALGLKSH SEGLKVVKLY QNGIRPKGVA TLIHYGLQYL KNLEILDLQD 

       250        260        270        280        290        300 
NTFTKHASLI LAKALPTWKD SLFELNLNDC LLKTAGSDEV FKVFTEVKFP NLHVLKFEYN 

       310        320        330        340        350        360 
EMAQETIEVS FLPAMEKGNL PELEKLEING NRLDEDSDAL DLLQSKFDDL EVDDFEEVDS 

       370        380        390        400 
EDEEGEDEED EDEDEKLEEI ETERLEKELL EVQVDDLAER LAETEIK 

« Hide

References

« Hide 'large scale' references
[1]"Structural and functional analyses of Saccharomyces cerevisiae wild-type and mutant RNA1 genes."
Traglia H.M., Atkinson N.S., Hopper A.K.
Mol. Cell. Biol. 9:2989-2999(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.
[2]Koh S.S., Young S.R., Young H.S., Hyen S.K.
Submitted (DEC-1989) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SL560-3A.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]Crouch R.J.
Submitted (JAN-1991) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 188-407.
Strain: ATCC 204510 / AB320.
[6]"The yeast RNA1 protein, necessary for RNA processing, is homologous to the human ribonuclease/angiogenin inhibitor (RAI)."
Schneider R., Schweiger M.
Mol. Gen. Genet. 233:315-318(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAINS LEUCINE-RICH REPEATS.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-360, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-360, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M27143 Genomic DNA. Translation: AAA34985.1.
M27142 Genomic DNA. Translation: AAA34983.1.
X17376 Genomic DNA. Translation: CAA35248.1. Frameshift.
Z49939 Genomic DNA. Translation: CAA90206.1.
X57160 Genomic DNA. Translation: CAA40449.1.
BK006946 Genomic DNA. Translation: DAA10135.1.
PIRBVBYN1. A32492.
RefSeqNP_013962.1. NM_001182742.1.

3D structure databases

ProteinModelPortalP11745.
SMRP11745. Positions 16-359.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35413. 147 interactions.
DIPDIP-2509N.
IntActP11745. 7 interactions.
MINTMINT-558123.
STRING4932.YMR235C.

Proteomic databases

MaxQBP11745.
PaxDbP11745.
PeptideAtlasP11745.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR235C; YMR235C; YMR235C.
GeneID855275.
KEGGsce:YMR235C.

Organism-specific databases

CYGDYMR235c.
SGDS000004848. RNA1.

Phylogenomic databases

eggNOGCOG5238.
GeneTreeENSGT00440000039203.
HOGENOMHOG000195026.
KOK14319.
OMAFTIMGST.
OrthoDBEOG71ZPB0.

Enzyme and pathway databases

BioCycYEAST:G3O-32916-MONOMER.

Gene expression databases

GenevestigatorP11745.

Family and domain databases

InterProIPR027038. RanGap.
[Graphical view]
PANTHERPTHR24113. PTHR24113. 1 hit.
ProtoNetSearch...

Other

NextBio978897.
PROP11745.

Entry information

Entry nameRNA1_YEAST
AccessionPrimary (citable) accession number: P11745
Secondary accession number(s): D6W061
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: April 1, 1990
Last modified: June 11, 2014
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families