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P11745

- RNA1_YEAST

UniProt

P11745 - RNA1_YEAST

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Protein
Ran GTPase-activating protein 1
Gene
RNA1, YMR235C, YM9959.17C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

GTPase activator for the nuclear Ras-related regulatory protein GSP1 (Ran), converting it to the putatively inactive GDP-bound state.

GO - Molecular functioni

  1. Ran GTPase activator activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. RNA import into nucleus Source: SGD
  2. chromatin silencing at telomere Source: SGD
  3. positive regulation of Ran GTPase activity Source: GOC
  4. protein import into nucleus Source: SGD
  5. rRNA export from nucleus Source: SGD
  6. ribosomal subunit export from nucleus Source: SGD
  7. tRNA export from nucleus Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

BioCyciYEAST:G3O-32916-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ran GTPase-activating protein 1
Alternative name(s):
Protein involved in RNA production/processing
Gene namesi
Name:RNA1
Ordered Locus Names:YMR235C
ORF Names:YM9959.17C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR235c.
SGDiS000004848. RNA1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Causes conditional lethality. Strains bearing this mutation do not grow at temperatures exceeding 30 degrees Celsius.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 407407Ran GTPase-activating protein 1
PRO_0000056742Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei360 – 3601Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP11745.
PaxDbiP11745.
PeptideAtlasiP11745.

Expressioni

Gene expression databases

GenevestigatoriP11745.

Interactioni

Protein-protein interaction databases

BioGridi35413. 147 interactions.
DIPiDIP-2509N.
IntActiP11745. 7 interactions.
MINTiMINT-558123.
STRINGi4932.YMR235C.

Structurei

3D structure databases

ProteinModelPortaliP11745.
SMRiP11745. Positions 16-359.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati11 – 3929LRR 1
Add
BLAST
Repeati40 – 6728LRR 2
Add
BLAST
Repeati68 – 10134LRR 3
Add
BLAST
Repeati102 – 13332LRR 4
Add
BLAST
Repeati134 – 16633LRR 5
Add
BLAST
Repeati167 – 19731LRR 6
Add
BLAST
Repeati198 – 22629LRR 7
Add
BLAST
Repeati227 – 25630LRR 8
Add
BLAST
Repeati257 – 28529LRR 9
Add
BLAST
Repeati286 – 31530LRR 10
Add
BLAST
Repeati316 – 34631LRR 11
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi348 – 37528Asp/Glu-rich (acidic)
Add
BLAST

Sequence similaritiesi

Belongs to the RNA1 family.

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiCOG5238.
GeneTreeiENSGT00440000039203.
HOGENOMiHOG000195026.
KOiK14319.
OMAiFTIMGST.
OrthoDBiEOG71ZPB0.

Family and domain databases

InterProiIPR027038. RanGap.
[Graphical view]
PANTHERiPTHR24113. PTHR24113. 1 hit.

Sequencei

Sequence statusi: Complete.

P11745-1 [UniParc]FASTAAdd to Basket

« Hide

MATLHFVPQH EEEQVYSISG KALKLTTSDD IKPYLEELAA LKTCTKLDLS    50
GNTIGTEASE ALAKCIAENT QVRESLVEVN FADLYTSRLV DEVVDSLKFL 100
LPVLLKCPHL EIVNLSDNAF GLRTIELLED YIAHAVNIKH LILSNNGMGP 150
FAGERIGKAL FHLAQNKKAA SKPFLETFIC GRNRLENGSA VYLALGLKSH 200
SEGLKVVKLY QNGIRPKGVA TLIHYGLQYL KNLEILDLQD NTFTKHASLI 250
LAKALPTWKD SLFELNLNDC LLKTAGSDEV FKVFTEVKFP NLHVLKFEYN 300
EMAQETIEVS FLPAMEKGNL PELEKLEING NRLDEDSDAL DLLQSKFDDL 350
EVDDFEEVDS EDEEGEDEED EDEDEKLEEI ETERLEKELL EVQVDDLAER 400
LAETEIK 407
Length:407
Mass (Da):45,816
Last modified:April 1, 1990 - v2
Checksum:iD77029B6783FFF09
GO

Sequence cautioni

The sequence CAA35248.1 differs from that shown. Reason: Frameshift at positions 185 and 203.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti17 – 171S → F in mutant RNA1-1.
Natural varianti194 – 1941A → V in mutant RNA1-1.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti42 – 421K → E in CAA35248. 1 Publication
Sequence conflicti222 – 2221L → S in CAA40449. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27143 Genomic DNA. Translation: AAA34985.1.
M27142 Genomic DNA. Translation: AAA34983.1.
X17376 Genomic DNA. Translation: CAA35248.1. Frameshift.
Z49939 Genomic DNA. Translation: CAA90206.1.
X57160 Genomic DNA. Translation: CAA40449.1.
BK006946 Genomic DNA. Translation: DAA10135.1.
PIRiA32492. BVBYN1.
RefSeqiNP_013962.1. NM_001182742.1.

Genome annotation databases

EnsemblFungiiYMR235C; YMR235C; YMR235C.
GeneIDi855275.
KEGGisce:YMR235C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27143 Genomic DNA. Translation: AAA34985.1 .
M27142 Genomic DNA. Translation: AAA34983.1 .
X17376 Genomic DNA. Translation: CAA35248.1 . Frameshift.
Z49939 Genomic DNA. Translation: CAA90206.1 .
X57160 Genomic DNA. Translation: CAA40449.1 .
BK006946 Genomic DNA. Translation: DAA10135.1 .
PIRi A32492. BVBYN1.
RefSeqi NP_013962.1. NM_001182742.1.

3D structure databases

ProteinModelPortali P11745.
SMRi P11745. Positions 16-359.
ModBasei Search...

Protein-protein interaction databases

BioGridi 35413. 147 interactions.
DIPi DIP-2509N.
IntActi P11745. 7 interactions.
MINTi MINT-558123.
STRINGi 4932.YMR235C.

Proteomic databases

MaxQBi P11745.
PaxDbi P11745.
PeptideAtlasi P11745.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR235C ; YMR235C ; YMR235C .
GeneIDi 855275.
KEGGi sce:YMR235C.

Organism-specific databases

CYGDi YMR235c.
SGDi S000004848. RNA1.

Phylogenomic databases

eggNOGi COG5238.
GeneTreei ENSGT00440000039203.
HOGENOMi HOG000195026.
KOi K14319.
OMAi FTIMGST.
OrthoDBi EOG71ZPB0.

Enzyme and pathway databases

BioCyci YEAST:G3O-32916-MONOMER.

Miscellaneous databases

NextBioi 978897.
PROi P11745.

Gene expression databases

Genevestigatori P11745.

Family and domain databases

InterProi IPR027038. RanGap.
[Graphical view ]
PANTHERi PTHR24113. PTHR24113. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural and functional analyses of Saccharomyces cerevisiae wild-type and mutant RNA1 genes."
    Traglia H.M., Atkinson N.S., Hopper A.K.
    Mol. Cell. Biol. 9:2989-2999(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.
  2. Koh S.S., Young S.R., Young H.S., Hyen S.K.
    Submitted (DEC-1989) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SL560-3A.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Crouch R.J.
    Submitted (JAN-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 188-407.
    Strain: ATCC 204510 / AB320.
  6. "The yeast RNA1 protein, necessary for RNA processing, is homologous to the human ribonuclease/angiogenin inhibitor (RAI)."
    Schneider R., Schweiger M.
    Mol. Gen. Genet. 233:315-318(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAINS LEUCINE-RICH REPEATS.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-360, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-360, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRNA1_YEAST
AccessioniPrimary (citable) accession number: P11745
Secondary accession number(s): D6W061
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: April 1, 1990
Last modified: June 11, 2014
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 52200 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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