Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P11745

- RNA1_YEAST

UniProt

P11745 - RNA1_YEAST

Protein

Ran GTPase-activating protein 1

Gene

RNA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 145 (01 Oct 2014)
      Sequence version 2 (01 Apr 1990)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    GTPase activator for the nuclear Ras-related regulatory protein GSP1 (Ran), converting it to the putatively inactive GDP-bound state.

    GO - Molecular functioni

    1. Ran GTPase activator activity Source: SGD

    GO - Biological processi

    1. chromatin silencing at telomere Source: SGD
    2. positive regulation of Ran GTPase activity Source: GOC
    3. protein import into nucleus Source: SGD
    4. ribosomal subunit export from nucleus Source: SGD
    5. RNA import into nucleus Source: SGD
    6. rRNA export from nucleus Source: SGD
    7. tRNA export from nucleus Source: SGD

    Keywords - Molecular functioni

    GTPase activation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32916-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ran GTPase-activating protein 1
    Alternative name(s):
    Protein involved in RNA production/processing
    Gene namesi
    Name:RNA1
    Ordered Locus Names:YMR235C
    ORF Names:YM9959.17C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR235c.
    SGDiS000004848. RNA1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Causes conditional lethality. Strains bearing this mutation do not grow at temperatures exceeding 30 degrees Celsius.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 407407Ran GTPase-activating protein 1PRO_0000056742Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei360 – 3601Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP11745.
    PaxDbiP11745.
    PeptideAtlasiP11745.

    Expressioni

    Gene expression databases

    GenevestigatoriP11745.

    Interactioni

    Protein-protein interaction databases

    BioGridi35413. 147 interactions.
    DIPiDIP-2509N.
    IntActiP11745. 7 interactions.
    MINTiMINT-558123.
    STRINGi4932.YMR235C.

    Structurei

    3D structure databases

    ProteinModelPortaliP11745.
    SMRiP11745. Positions 16-359.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati11 – 3929LRR 1Add
    BLAST
    Repeati40 – 6728LRR 2Add
    BLAST
    Repeati68 – 10134LRR 3Add
    BLAST
    Repeati102 – 13332LRR 4Add
    BLAST
    Repeati134 – 16633LRR 5Add
    BLAST
    Repeati167 – 19731LRR 6Add
    BLAST
    Repeati198 – 22629LRR 7Add
    BLAST
    Repeati227 – 25630LRR 8Add
    BLAST
    Repeati257 – 28529LRR 9Add
    BLAST
    Repeati286 – 31530LRR 10Add
    BLAST
    Repeati316 – 34631LRR 11Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi348 – 37528Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the RNA1 family.Curated
    Contains 11 LRR (leucine-rich) repeats.Curated

    Keywords - Domaini

    Leucine-rich repeat, Repeat

    Phylogenomic databases

    eggNOGiCOG5238.
    GeneTreeiENSGT00440000039203.
    HOGENOMiHOG000195026.
    KOiK14319.
    OMAiFTIMGST.
    OrthoDBiEOG71ZPB0.

    Family and domain databases

    InterProiIPR027038. RanGap.
    [Graphical view]
    PANTHERiPTHR24113. PTHR24113. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P11745-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATLHFVPQH EEEQVYSISG KALKLTTSDD IKPYLEELAA LKTCTKLDLS    50
    GNTIGTEASE ALAKCIAENT QVRESLVEVN FADLYTSRLV DEVVDSLKFL 100
    LPVLLKCPHL EIVNLSDNAF GLRTIELLED YIAHAVNIKH LILSNNGMGP 150
    FAGERIGKAL FHLAQNKKAA SKPFLETFIC GRNRLENGSA VYLALGLKSH 200
    SEGLKVVKLY QNGIRPKGVA TLIHYGLQYL KNLEILDLQD NTFTKHASLI 250
    LAKALPTWKD SLFELNLNDC LLKTAGSDEV FKVFTEVKFP NLHVLKFEYN 300
    EMAQETIEVS FLPAMEKGNL PELEKLEING NRLDEDSDAL DLLQSKFDDL 350
    EVDDFEEVDS EDEEGEDEED EDEDEKLEEI ETERLEKELL EVQVDDLAER 400
    LAETEIK 407
    Length:407
    Mass (Da):45,816
    Last modified:April 1, 1990 - v2
    Checksum:iD77029B6783FFF09
    GO

    Sequence cautioni

    The sequence CAA35248.1 differs from that shown. Reason: Frameshift at positions 185 and 203.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti42 – 421K → E in CAA35248. 1 PublicationCurated
    Sequence conflicti222 – 2221L → S in CAA40449. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti17 – 171S → F in mutant RNA1-1.
    Natural varianti194 – 1941A → V in mutant RNA1-1.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27143 Genomic DNA. Translation: AAA34985.1.
    M27142 Genomic DNA. Translation: AAA34983.1.
    X17376 Genomic DNA. Translation: CAA35248.1. Frameshift.
    Z49939 Genomic DNA. Translation: CAA90206.1.
    X57160 Genomic DNA. Translation: CAA40449.1.
    BK006946 Genomic DNA. Translation: DAA10135.1.
    PIRiA32492. BVBYN1.
    RefSeqiNP_013962.1. NM_001182742.1.

    Genome annotation databases

    EnsemblFungiiYMR235C; YMR235C; YMR235C.
    GeneIDi855275.
    KEGGisce:YMR235C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27143 Genomic DNA. Translation: AAA34985.1 .
    M27142 Genomic DNA. Translation: AAA34983.1 .
    X17376 Genomic DNA. Translation: CAA35248.1 . Frameshift.
    Z49939 Genomic DNA. Translation: CAA90206.1 .
    X57160 Genomic DNA. Translation: CAA40449.1 .
    BK006946 Genomic DNA. Translation: DAA10135.1 .
    PIRi A32492. BVBYN1.
    RefSeqi NP_013962.1. NM_001182742.1.

    3D structure databases

    ProteinModelPortali P11745.
    SMRi P11745. Positions 16-359.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35413. 147 interactions.
    DIPi DIP-2509N.
    IntActi P11745. 7 interactions.
    MINTi MINT-558123.
    STRINGi 4932.YMR235C.

    Proteomic databases

    MaxQBi P11745.
    PaxDbi P11745.
    PeptideAtlasi P11745.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR235C ; YMR235C ; YMR235C .
    GeneIDi 855275.
    KEGGi sce:YMR235C.

    Organism-specific databases

    CYGDi YMR235c.
    SGDi S000004848. RNA1.

    Phylogenomic databases

    eggNOGi COG5238.
    GeneTreei ENSGT00440000039203.
    HOGENOMi HOG000195026.
    KOi K14319.
    OMAi FTIMGST.
    OrthoDBi EOG71ZPB0.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32916-MONOMER.

    Miscellaneous databases

    NextBioi 978897.
    PROi P11745.

    Gene expression databases

    Genevestigatori P11745.

    Family and domain databases

    InterProi IPR027038. RanGap.
    [Graphical view ]
    PANTHERi PTHR24113. PTHR24113. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structural and functional analyses of Saccharomyces cerevisiae wild-type and mutant RNA1 genes."
      Traglia H.M., Atkinson N.S., Hopper A.K.
      Mol. Cell. Biol. 9:2989-2999(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.
    2. Koh S.S., Young S.R., Young H.S., Hyen S.K.
      Submitted (DEC-1989) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SL560-3A.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Crouch R.J.
      Submitted (JAN-1991) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 188-407.
      Strain: ATCC 204510 / AB320.
    6. "The yeast RNA1 protein, necessary for RNA processing, is homologous to the human ribonuclease/angiogenin inhibitor (RAI)."
      Schneider R., Schweiger M.
      Mol. Gen. Genet. 233:315-318(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAINS LEUCINE-RICH REPEATS.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-360, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-360, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRNA1_YEAST
    AccessioniPrimary (citable) accession number: P11745
    Secondary accession number(s): D6W061
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 145 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 52200 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3