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Protein

Methylated-DNA--protein-cysteine methyltransferase, constitutive

Gene

ogt

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.UniRule annotation1 Publication

Miscellaneous

Bacillus subtilis contains 2 species of O-6-methylguanine-DNA alkyltransferases. Ogt and adaB are regulated in different ways, constitutively and inducibly on adaptive treatment, respectively.
This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.UniRule annotation1 Publication
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei130Nucleophile; methyl group acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciBSUB:BSU13540-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferase, constitutiveUniRule annotation (EC:2.1.1.63UniRule annotation1 Publication)
Alternative name(s):
6-O-methylguanine-DNA methyltransferaseUniRule annotation
Short name:
MGMTUniRule annotation
O-6-methylguanine-DNA-alkyltransferaseUniRule annotation
Gene namesi
Name:ogtUniRule annotation
Synonyms:dat, dat1
Ordered Locus Names:BSU13540
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393641 – 165Methylated-DNA--protein-cysteine methyltransferase, constitutiveAdd BLAST165

Proteomic databases

PaxDbiP11742.

Expressioni

Inductioni

Constitutively expressed.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100007501.

Structurei

3D structure databases

ProteinModelPortaliP11742.
SMRiP11742.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105K85. Bacteria.
COG0350. LUCA.
HOGENOMiHOG000244137.
InParanoidiP11742.
KOiK00567.
OMAiYGESRSY.
PhylomeDBiP11742.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00772. OGT. 1 hit.
InterProiView protein in InterPro
IPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR036217. MethylDNA_cys_MeTrfase_DNAb.
IPR008332. MethylG_MeTrfase_N.
IPR023546. MGMT.
IPR036631. MGMT_N_sf.
IPR036388. WH-like_DNA-bd_sf.
PfamiView protein in Pfam
PF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiView protein in PROSITE
PS00374. MGMT. 1 hit.

Sequencei

Sequence statusi: Complete.

P11742-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYYTTAETP LGELIIAEEE DRITRLFLSQ EDWVDWKETV QNTEHKETPN
60 70 80 90 100
LAEAKQQLQE YFAGERKTFS LPLSQKGTPF QQKVWQALER IPYGESRSYA
110 120 130 140 150
DIAAAVGSPK AVRAVGQANK RNDLPIFVPC HRVIGKNSAL TGYAGSKTEI
160
KAFLLNIERI SYKEK
Length:165
Mass (Da):18,752
Last modified:October 1, 1989 - v1
Checksum:i3B52393F34B5EE9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15659 Genomic DNA. Translation: CAA33694.1.
AL009126 Genomic DNA. Translation: CAB13227.1.
PIRiS04877. XUBSM1.
RefSeqiNP_389237.1. NC_000964.3.
WP_003232502.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13227; CAB13227; BSU13540.
GeneIDi939332.
KEGGibsu:BSU13540.
PATRICifig|224308.179.peg.1470.

Similar proteinsi

Entry informationi

Entry nameiOGT_BACSU
AccessioniPrimary (citable) accession number: P11742
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: February 28, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome