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Protein

Dihydrofolate reductase type 5

Gene

dhfrV

Organism
Escherichia coli
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Miscellaneous

Confers trimethoprim resistance.

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase type 8 (dhfrVIII), Dihydrofolate reductase type 9 (dhfrIX), Dihydrofolate reductase (BTQ06_28630), Dihydrofolate reductase, Dihydrofolate reductase (APZ14_00990), Dihydrofolate reductase (dfrB2), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase type A10 (dfrA10), Dihydrofolate reductase (dfrA21), Dihydrofolate reductase (dfr2d), Dihydrofolate reductase type 2, Dihydrofolate reductase type 2, Dihydrofolate reductase (dfrB3), Dihydrofolate reductase type 1 (dhfrI), Dihydrofolate reductase type 5 (dhfrV), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase type 15 (dhfrXV), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfrII), Dihydrofolate reductase (folA), Dihydrofolate reductase (BHS87_26495), Dihydrofolate reductase (ECs0051), Dihydrofolate reductase (dfrA26), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (dfr2d), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase type A13 (dfrA13), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase type 2, Dihydrofolate reductase (CSB64_27845), Dihydrofolate reductase (dfr2a), Dihydrofolate reductase type 7 (dhfrVII), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfr22), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfr22), Dihydrofolate reductase (dfrA12)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAntibiotic resistance, Methotrexate resistance, One-carbon metabolism, Trimethoprim resistance
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase type 5 (EC:1.5.1.3)
Alternative name(s):
Dihydrofolate reductase type V
Gene namesi
Name:dhfrV
Encoded oniPlasmid pLMO200 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001864221 – 157Dihydrofolate reductase type 5Add BLAST157

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP11731
SMRiP11731
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 156DHFRPROSITE-ProRule annotationAdd BLAST155

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

KOiK18589

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.10, 1 hit
InterProiView protein in InterPro
IPR012259 DHFR
IPR024072 DHFR-like_dom_sf
IPR017925 DHFR_CS
IPR001796 DHFR_dom
PANTHERiPTHR22778:SF16 PTHR22778:SF16, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P11731-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVSLMAAKA KNGVIGCGPH IPWSAKGEQL LFKALTYNQW LLVGRKTFES
60 70 80 90 100
MGALPNRKYA VVTRSAWTAD NDNVIVFPSI EEAMYGLAEL TDHVIVSGGG
110 120 130 140 150
EIYRETLPMA STLHISTIDI EPEGDVFFPN IPNTFEVVFE QHFSSNINYC

YQIWQKG
Length:157
Mass (Da):17,531
Last modified:October 1, 1989 - v1
Checksum:i04B968163A25EFF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12868 Genomic DNA Translation: CAA31356.1
PIRiS04810
RefSeqiWP_001317507.1, NZ_NXOL01000089.1
YP_008864016.1, NC_022992.1
YP_008864683.1, NC_022996.1

Genome annotation databases

GeneIDi17824297
17824432
KEGGiag:CAA31356

Similar proteinsi

Entry informationi

Entry nameiDYR5_ECOLX
AccessioniPrimary (citable) accession number: P11731
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

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