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Protein

Dihydrofolate reductase type 5

Gene

dhfrV

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Miscellaneous

Confers trimethoprim resistance.

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase type 8 (dhfrVIII), Dihydrofolate reductase (BHS87_26495), Dihydrofolate reductase type 9 (dhfrIX), Dihydrofolate reductase (ECs0051), Dihydrofolate reductase, Dihydrofolate reductase (APZ14_00990), Dihydrofolate reductase (dfrB2), Dihydrofolate reductase (dfrA26), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase type A10 (dfrA10), Dihydrofolate reductase (dfrA21), Dihydrofolate reductase (dfr2d), Dihydrofolate reductase (dfr2d), Dihydrofolate reductase (folA), Dihydrofolate reductase type A13 (dfrA13), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase type 2, Dihydrofolate reductase type 2, Dihydrofolate reductase (dfr2a), Dihydrofolate reductase type 7 (dhfrVII), Dihydrofolate reductase type 2, Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfrB3), Dihydrofolate reductase type 1 (dhfrI), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfr22), Dihydrofolate reductase type 5 (dhfrV), Dihydrofolate reductase (dhfrXII), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase type 15 (dhfrXV), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfrII), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfr22), Dihydrofolate reductase (dfrA12)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAntibiotic resistance, Methotrexate resistance, One-carbon metabolism, Trimethoprim resistance
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase type 5 (EC:1.5.1.3)
Alternative name(s):
Dihydrofolate reductase type V
Gene namesi
Name:dhfrV
Encoded oniPlasmid pLMO200 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001864221 – 157Dihydrofolate reductase type 5Add BLAST157

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP11731.
SMRiP11731.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 156DHFRPROSITE-ProRule annotationAdd BLAST155

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

KOiK18589.

Family and domain databases

CDDicd00209. DHFR. 1 hit.
Gene3Di3.40.430.10. 1 hit.
InterProiView protein in InterPro
IPR012259. DHFR.
IPR024072. DHFR-like_dom_sf.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
PfamiView protein in Pfam
PF00186. DHFR_1. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiView protein in PROSITE
PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P11731-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVSLMAAKA KNGVIGCGPH IPWSAKGEQL LFKALTYNQW LLVGRKTFES
60 70 80 90 100
MGALPNRKYA VVTRSAWTAD NDNVIVFPSI EEAMYGLAEL TDHVIVSGGG
110 120 130 140 150
EIYRETLPMA STLHISTIDI EPEGDVFFPN IPNTFEVVFE QHFSSNINYC

YQIWQKG
Length:157
Mass (Da):17,531
Last modified:October 1, 1989 - v1
Checksum:i04B968163A25EFF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12868 Genomic DNA. Translation: CAA31356.1.
PIRiS04810.
RefSeqiWP_001317507.1. NZ_MQAE01000066.1.
YP_008864016.1. NC_022992.1.
YP_008864683.1. NC_022996.1.

Genome annotation databases

GeneIDi17824297.
17824432.
KEGGiag:CAA31356.

Similar proteinsi

Entry informationi

Entry nameiDYR5_ECOLX
AccessioniPrimary (citable) accession number: P11731
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 22, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families