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P11724 (OTC_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ornithine carbamoyltransferase
Short name=OTCase
EC=2.1.3.3
Gene names
Name:argF
Ordered Locus Names:PA3537
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase, the enzyme involved in the final step in arginine biosynthesis. Ref.3

Catalytic activity

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP-Rule MF_01109

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3. HAMAP-Rule MF_01109

Subunit structure

Homotrimer. Ref.3

Subcellular location

Cytoplasm HAMAP-Rule MF_01109.

Sequence similarities

Belongs to the ATCase/OTCase family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   Molecular functionTransferase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionamino acid binding

Inferred from electronic annotation. Source: InterPro

ornithine carbamoyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 305304Ornithine carbamoyltransferase HAMAP-Rule MF_01109
PRO_0000112985

Regions

Region53 – 575Carbamoyl phosphate binding By similarity
Region131 – 1344Carbamoyl phosphate binding By similarity
Region223 – 2242Ornithine binding By similarity
Region258 – 2614Carbamoyl phosphate binding By similarity

Sites

Binding site71Carbamoyl phosphate By similarity
Binding site691Carbamoyl phosphate By similarity
Binding site801Carbamoyl phosphate By similarity
Binding site1041Carbamoyl phosphate By similarity
Binding site1621Ornithine By similarity
Binding site2191Ornithine By similarity
Binding site2691Carbamoyl phosphate By similarity
Binding site2871Carbamoyl phosphate By similarity
Site281Important for structural integrity By similarity
Site1441Important for structural integrity By similarity

Experimental info

Sequence conflict170 – 1723AAL → EEM in AAA25720. Ref.1
Sequence conflict2361L → M in AAA25720. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P11724 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 6C196BC06BD27F0B

FASTA30533,925
        10         20         30         40         50         60 
MSVRHFLSFM DYSPEELIGL IRRGSELKDL RNRGVLYEPL KSRVLGMVFE KASTRTRLSF 

        70         80         90        100        110        120 
EAGMIQLGGQ AIFLSPRDTQ LGRGEPIGDS ARVMSRMLDG VMIRTFAHAT LTEFAAHSKV 

       130        140        150        160        170        180 
PVINGLSDDL HPCQLLADMQ TFHEHRGSIQ GKTVAWIGDG NNMCNSYIEA ALKFDFQLRV 

       190        200        210        220        230        240 
ACPEGYEPKA EFVALAGDRL RVVRDPREAV AGAHLVSTDV WASMGQEDEA AARIALFRPY 

       250        260        270        280        290        300 
QVNAALLDGA ADDVLFMHCL PAHRGEEISE ELLDDPRSVA WDQAENRLHA QKALLELLIE 


HAHYA

« Hide

References

« Hide 'large scale' references
[1]"Anabolic ornithine carbamoyltransferase of Pseudomonas aeruginosa: nucleotide sequence and transcriptional control of the argF structural gene."
Itoh Y., Soldati L., Stalon V., Falmagne P., Terawaki Y., Leisinger T., Haas D.
J. Bacteriol. 170:2725-2734(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-38.
Strain: PAO.
[2]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]"The occurrence of a catabolic and an anabolic ornithine carbamoyltransferase in Pseudomonas."
Stalon V., Ramos F., Pierard A., Wiame J.M.
Biochim. Biophys. Acta 139:91-97(1967) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS AN ANABOLIC OTCASE, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M19939 Genomic DNA. Translation: AAA25720.1.
AE004091 Genomic DNA. Translation: AAG06925.1.
PIROWPSAA. A32013.
E83203.
RefSeqNP_252227.1. NC_002516.2.

3D structure databases

ProteinModelPortalP11724.
SMRP11724. Positions 3-299.
ModBaseSearch...

Protein-protein interaction databases

STRING208964.PA3537.

Protocols and materials databases

DNASU878793.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID878793.
KEGGpae:PA3537.
PATRIC19841687. VBIPseAer58763_3701.

Organism-specific databases

PseudoCAPPA3537.

Phylogenomic databases

eggNOGCOG0078.
HOGENOMHOG000022686.
KOK00611.
OMAIMIRTTE.
ProtClustDBPRK00779.

Enzyme and pathway databases

SABIO-RKP11724.
UniPathwayUPA00068; UER00112.

Family and domain databases

Gene3D3.40.50.1370. 2 hits.
HAMAPMF_01109. OTCase.
InterProIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. Orn_carbamltrans.
[Graphical view]
PfamPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMSSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMsTIGR00658. orni_carb_tr. 1 hit.
PROSITEPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameOTC_PSEAE
AccessionPrimary (citable) accession number: P11724
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: May 29, 2013
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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