P11724 (OTC_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ornithine carbamoyltransferase
Short name=OTCase
EC=2.1.3.3

Gene names

Name:	argF
Ordered Locus Names:	PA3537

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3. HAMAP MF_01109
Subunit structure	Homotrimer.
Subcellular location	Cytoplasm HAMAP MF_01109.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 305	304	Ornithine carbamoyltransferase HAMAP MF_01109		PRO_0000112985
Regions
	Region	53 – 57	5	Carbamoyl phosphate binding By similarity
	Region	258 – 261	4	Ornithine binding By similarity
Sites
	Binding site	104	1	Carbamoyl phosphate By similarity
	Binding site	131	1	Carbamoyl phosphate By similarity
	Site	28	1	Important for structural integrity By similarity
	Site	144	1	Important for structural integrity By similarity
Experimental info
	Sequence conflict	170 – 172	3	AAL → EEM in AAA25720. Ref.1
	Sequence conflict	236	1	L → M in AAA25720. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P11724 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 6C196BC06BD27F0B	FASTA	305	33,925
	10 20 30 40 50 60 MSVRHFLSFM DYSPEELIGL IRRGSELKDL RNRGVLYEPL KSRVLGMVFE KASTRTRLSF 70 80 90 100 110 120 EAGMIQLGGQ AIFLSPRDTQ LGRGEPIGDS ARVMSRMLDG VMIRTFAHAT LTEFAAHSKV 130 140 150 160 170 180 PVINGLSDDL HPCQLLADMQ TFHEHRGSIQ GKTVAWIGDG NNMCNSYIEA ALKFDFQLRV 190 200 210 220 230 240 ACPEGYEPKA EFVALAGDRL RVVRDPREAV AGAHLVSTDV WASMGQEDEA AARIALFRPY 250 260 270 280 290 300 QVNAALLDGA ADDVLFMHCL PAHRGEEISE ELLDDPRSVA WDQAENRLHA QKALLELLIE HAHYA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Anabolic ornithine carbamoyltransferase of Pseudomonas aeruginosa: nucleotide sequence and transcriptional control of the argF structural gene." Itoh Y., Soldati L., Stalon V., Falmagne P., Terawaki Y., Leisinger T., Haas D. J. Bacteriol. 170:2725-2734(1988) [PubMed: 3131308] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-38. Strain: PAO.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M19939 Genomic DNA. Translation: AAA25720.1. AE004091 Genomic DNA. Translation: AAG06925.1.
PIR	OWPSAA. A32013. E83203.
RefSeq	NP_252227.1. NC_002516.2.
3D structure databases
ProteinModelPortal	P11724.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	878793.
GenomeReviews	Gene locus PA3537 in contig AE004091_GR.
KEGG	pae:PA3537.
PATRIC	19841687. VBIPseAer58763_3701.
Organism-specific databases
PseudoCAP	PA3537.
Phylogenomic databases
HOGENOM	HBG579429.
OMA	GVDCVVT.
ProtClustDB	PRK00779.
Enzyme and pathway databases
BioCyc	PAER208964:PA3537-MONOMER.
Family and domain databases
HAMAP	MF_01109. OTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024904. Orn_carbamltrans. [Graphical view]
KO	K00611.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTC_PSEAE

Accession

Primary (citable) accession number: P11724

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 96 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program