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P11724

- OTC_PSEAE

UniProt

P11724 - OTC_PSEAE

Protein

Ornithine carbamoyltransferase

Gene

argF

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase, the enzyme involved in the final step in arginine biosynthesis.1 Publication

    Catalytic activityi

    Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei7 – 71Carbamoyl phosphateBy similarity
    Sitei28 – 281Important for structural integrityBy similarity
    Binding sitei69 – 691Carbamoyl phosphateBy similarity
    Binding sitei80 – 801Carbamoyl phosphateBy similarity
    Binding sitei104 – 1041Carbamoyl phosphateBy similarity
    Sitei144 – 1441Important for structural integrityBy similarity
    Binding sitei162 – 1621OrnithineBy similarity
    Binding sitei219 – 2191OrnithineBy similarity
    Binding sitei269 – 2691Carbamoyl phosphateBy similarity
    Binding sitei287 – 2871Carbamoyl phosphateBy similarity

    GO - Molecular functioni

    1. amino acid binding Source: InterPro
    2. ornithine carbamoyltransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. arginine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Arginine biosynthesis

    Enzyme and pathway databases

    SABIO-RKP11724.
    UniPathwayiUPA00068; UER00112.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ornithine carbamoyltransferase (EC:2.1.3.3)
    Short name:
    OTCase
    Gene namesi
    Name:argF
    Ordered Locus Names:PA3537
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA3537.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 305304Ornithine carbamoyltransferasePRO_0000112985Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.1 Publication

    Protein-protein interaction databases

    STRINGi208964.PA3537.

    Structurei

    3D structure databases

    ProteinModelPortaliP11724.
    SMRiP11724. Positions 3-299.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni53 – 575Carbamoyl phosphate bindingBy similarity
    Regioni131 – 1344Carbamoyl phosphate bindingBy similarity
    Regioni223 – 2242Ornithine bindingBy similarity
    Regioni258 – 2614Carbamoyl phosphate bindingBy similarity

    Sequence similaritiesi

    Belongs to the ATCase/OTCase family.Curated

    Phylogenomic databases

    eggNOGiCOG0078.
    HOGENOMiHOG000022686.
    KOiK00611.
    OMAiEAKKGFY.
    OrthoDBiEOG690MGV.
    PhylomeDBiP11724.

    Family and domain databases

    Gene3Di3.40.50.1370. 2 hits.
    HAMAPiMF_01109. OTCase.
    InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
    IPR006130. Asp/Orn_carbamoylTrfase.
    IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
    IPR002292. Orn/put_carbamltrans.
    IPR024904. Orn_carbamltrans.
    [Graphical view]
    PfamiPF00185. OTCace. 1 hit.
    PF02729. OTCace_N. 1 hit.
    [Graphical view]
    PRINTSiPR00100. AOTCASE.
    PR00102. OTCASE.
    SUPFAMiSSF53671. SSF53671. 1 hit.
    TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
    PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P11724-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVRHFLSFM DYSPEELIGL IRRGSELKDL RNRGVLYEPL KSRVLGMVFE    50
    KASTRTRLSF EAGMIQLGGQ AIFLSPRDTQ LGRGEPIGDS ARVMSRMLDG 100
    VMIRTFAHAT LTEFAAHSKV PVINGLSDDL HPCQLLADMQ TFHEHRGSIQ 150
    GKTVAWIGDG NNMCNSYIEA ALKFDFQLRV ACPEGYEPKA EFVALAGDRL 200
    RVVRDPREAV AGAHLVSTDV WASMGQEDEA AARIALFRPY QVNAALLDGA 250
    ADDVLFMHCL PAHRGEEISE ELLDDPRSVA WDQAENRLHA QKALLELLIE 300
    HAHYA 305
    Length:305
    Mass (Da):33,925
    Last modified:January 23, 2007 - v3
    Checksum:i6C196BC06BD27F0B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti170 – 1723AAL → EEM in AAA25720. (PubMed:3131308)Curated
    Sequence conflicti236 – 2361L → M in AAA25720. (PubMed:3131308)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M19939 Genomic DNA. Translation: AAA25720.1.
    AE004091 Genomic DNA. Translation: AAG06925.1.
    PIRiA32013. OWPSAA.
    E83203.
    RefSeqiNP_252227.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG06925; AAG06925; PA3537.
    GeneIDi878793.
    KEGGipae:PA3537.
    PATRICi19841687. VBIPseAer58763_3701.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M19939 Genomic DNA. Translation: AAA25720.1 .
    AE004091 Genomic DNA. Translation: AAG06925.1 .
    PIRi A32013. OWPSAA.
    E83203.
    RefSeqi NP_252227.1. NC_002516.2.

    3D structure databases

    ProteinModelPortali P11724.
    SMRi P11724. Positions 3-299.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA3537.

    Protocols and materials databases

    DNASUi 878793.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG06925 ; AAG06925 ; PA3537 .
    GeneIDi 878793.
    KEGGi pae:PA3537.
    PATRICi 19841687. VBIPseAer58763_3701.

    Organism-specific databases

    PseudoCAPi PA3537.

    Phylogenomic databases

    eggNOGi COG0078.
    HOGENOMi HOG000022686.
    KOi K00611.
    OMAi EAKKGFY.
    OrthoDBi EOG690MGV.
    PhylomeDBi P11724.

    Enzyme and pathway databases

    UniPathwayi UPA00068 ; UER00112 .
    SABIO-RK P11724.

    Family and domain databases

    Gene3Di 3.40.50.1370. 2 hits.
    HAMAPi MF_01109. OTCase.
    InterProi IPR006132. Asp/Orn_carbamoyltranf_P-bd.
    IPR006130. Asp/Orn_carbamoylTrfase.
    IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
    IPR002292. Orn/put_carbamltrans.
    IPR024904. Orn_carbamltrans.
    [Graphical view ]
    Pfami PF00185. OTCace. 1 hit.
    PF02729. OTCace_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00100. AOTCASE.
    PR00102. OTCASE.
    SUPFAMi SSF53671. SSF53671. 1 hit.
    TIGRFAMsi TIGR00658. orni_carb_tr. 1 hit.
    PROSITEi PS00097. CARBAMOYLTRANSFERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Anabolic ornithine carbamoyltransferase of Pseudomonas aeruginosa: nucleotide sequence and transcriptional control of the argF structural gene."
      Itoh Y., Soldati L., Stalon V., Falmagne P., Terawaki Y., Leisinger T., Haas D.
      J. Bacteriol. 170:2725-2734(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-38.
      Strain: PAO.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    3. "The occurrence of a catabolic and an anabolic ornithine carbamoyltransferase in Pseudomonas."
      Stalon V., Ramos F., Pierard A., Wiame J.M.
      Biochim. Biophys. Acta 139:91-97(1967) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS AN ANABOLIC OTCASE, SUBUNIT.

    Entry informationi

    Entry nameiOTC_PSEAE
    AccessioniPrimary (citable) accession number: P11724
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 119 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3