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P11724

- OTC_PSEAE

UniProt

P11724 - OTC_PSEAE

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Protein
Ornithine carbamoyltransferase
Gene
argF, PA3537
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase, the enzyme involved in the final step in arginine biosynthesis.1 Publication

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei7 – 71Carbamoyl phosphate By similarity
Sitei28 – 281Important for structural integrity By similarity
Binding sitei69 – 691Carbamoyl phosphate By similarity
Binding sitei80 – 801Carbamoyl phosphate By similarity
Binding sitei104 – 1041Carbamoyl phosphate By similarity
Sitei144 – 1441Important for structural integrity By similarity
Binding sitei162 – 1621Ornithine By similarity
Binding sitei219 – 2191Ornithine By similarity
Binding sitei269 – 2691Carbamoyl phosphate By similarity
Binding sitei287 – 2871Carbamoyl phosphate By similarity

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. ornithine carbamoyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

SABIO-RKP11724.
UniPathwayiUPA00068; UER00112.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase (EC:2.1.3.3)
Short name:
OTCase
Gene namesi
Name:argF
Ordered Locus Names:PA3537
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA3537.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 305304Ornithine carbamoyltransferaseUniRule annotation
PRO_0000112985Add
BLAST

Interactioni

Subunit structurei

Homotrimer.1 Publication

Protein-protein interaction databases

STRINGi208964.PA3537.

Structurei

3D structure databases

ProteinModelPortaliP11724.
SMRiP11724. Positions 3-299.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni53 – 575Carbamoyl phosphate binding By similarity
Regioni131 – 1344Carbamoyl phosphate binding By similarity
Regioni223 – 2242Ornithine binding By similarity
Regioni258 – 2614Carbamoyl phosphate binding By similarity

Sequence similaritiesi

Belongs to the ATCase/OTCase family.

Phylogenomic databases

eggNOGiCOG0078.
HOGENOMiHOG000022686.
KOiK00611.
OMAiEAKKGFY.
OrthoDBiEOG690MGV.
PhylomeDBiP11724.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. Orn_carbamltrans.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11724-1 [UniParc]FASTAAdd to Basket

« Hide

MSVRHFLSFM DYSPEELIGL IRRGSELKDL RNRGVLYEPL KSRVLGMVFE    50
KASTRTRLSF EAGMIQLGGQ AIFLSPRDTQ LGRGEPIGDS ARVMSRMLDG 100
VMIRTFAHAT LTEFAAHSKV PVINGLSDDL HPCQLLADMQ TFHEHRGSIQ 150
GKTVAWIGDG NNMCNSYIEA ALKFDFQLRV ACPEGYEPKA EFVALAGDRL 200
RVVRDPREAV AGAHLVSTDV WASMGQEDEA AARIALFRPY QVNAALLDGA 250
ADDVLFMHCL PAHRGEEISE ELLDDPRSVA WDQAENRLHA QKALLELLIE 300
HAHYA 305
Length:305
Mass (Da):33,925
Last modified:January 23, 2007 - v3
Checksum:i6C196BC06BD27F0B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti170 – 1723AAL → EEM in AAA25720. 1 Publication
Sequence conflicti236 – 2361L → M in AAA25720. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M19939 Genomic DNA. Translation: AAA25720.1.
AE004091 Genomic DNA. Translation: AAG06925.1.
PIRiA32013. OWPSAA.
E83203.
RefSeqiNP_252227.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG06925; AAG06925; PA3537.
GeneIDi878793.
KEGGipae:PA3537.
PATRICi19841687. VBIPseAer58763_3701.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M19939 Genomic DNA. Translation: AAA25720.1 .
AE004091 Genomic DNA. Translation: AAG06925.1 .
PIRi A32013. OWPSAA.
E83203.
RefSeqi NP_252227.1. NC_002516.2.

3D structure databases

ProteinModelPortali P11724.
SMRi P11724. Positions 3-299.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA3537.

Protocols and materials databases

DNASUi 878793.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG06925 ; AAG06925 ; PA3537 .
GeneIDi 878793.
KEGGi pae:PA3537.
PATRICi 19841687. VBIPseAer58763_3701.

Organism-specific databases

PseudoCAPi PA3537.

Phylogenomic databases

eggNOGi COG0078.
HOGENOMi HOG000022686.
KOi K00611.
OMAi EAKKGFY.
OrthoDBi EOG690MGV.
PhylomeDBi P11724.

Enzyme and pathway databases

UniPathwayi UPA00068 ; UER00112 .
SABIO-RK P11724.

Family and domain databases

Gene3Di 3.40.50.1370. 2 hits.
HAMAPi MF_01109. OTCase.
InterProi IPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. Orn_carbamltrans.
[Graphical view ]
Pfami PF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view ]
PRINTSi PR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMi SSF53671. SSF53671. 1 hit.
TIGRFAMsi TIGR00658. orni_carb_tr. 1 hit.
PROSITEi PS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Anabolic ornithine carbamoyltransferase of Pseudomonas aeruginosa: nucleotide sequence and transcriptional control of the argF structural gene."
    Itoh Y., Soldati L., Stalon V., Falmagne P., Terawaki Y., Leisinger T., Haas D.
    J. Bacteriol. 170:2725-2734(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-38.
    Strain: PAO.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  3. "The occurrence of a catabolic and an anabolic ornithine carbamoyltransferase in Pseudomonas."
    Stalon V., Ramos F., Pierard A., Wiame J.M.
    Biochim. Biophys. Acta 139:91-97(1967) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN ANABOLIC OTCASE, SUBUNIT.

Entry informationi

Entry nameiOTC_PSEAE
AccessioniPrimary (citable) accession number: P11724
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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