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Protein

Ornithine carbamoyltransferase

Gene

argF

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase, the enzyme involved in the final step in arginine biosynthesis.1 Publication

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei7 – 71Carbamoyl phosphateBy similarity
Sitei28 – 281Important for structural integrityBy similarity
Binding sitei69 – 691Carbamoyl phosphateBy similarity
Binding sitei80 – 801Carbamoyl phosphateBy similarity
Binding sitei104 – 1041Carbamoyl phosphateBy similarity
Sitei144 – 1441Important for structural integrityBy similarity
Binding sitei162 – 1621OrnithineBy similarity
Binding sitei219 – 2191OrnithineBy similarity
Binding sitei269 – 2691Carbamoyl phosphateBy similarity
Binding sitei287 – 2871Carbamoyl phosphateBy similarity

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. ornithine carbamoyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

SABIO-RKP11724.
UniPathwayiUPA00068; UER00112.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase (EC:2.1.3.3)
Short name:
OTCase
Gene namesi
Name:argF
Ordered Locus Names:PA3537
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA3537.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 305304Ornithine carbamoyltransferasePRO_0000112985Add
BLAST

Interactioni

Subunit structurei

Homotrimer.1 Publication

Protein-protein interaction databases

STRINGi208964.PA3537.

Structurei

3D structure databases

ProteinModelPortaliP11724.
SMRiP11724. Positions 4-299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni53 – 575Carbamoyl phosphate bindingBy similarity
Regioni131 – 1344Carbamoyl phosphate bindingBy similarity
Regioni223 – 2242Ornithine bindingBy similarity
Regioni258 – 2614Carbamoyl phosphate bindingBy similarity

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiCOG0078.
HOGENOMiHOG000022686.
InParanoidiP11724.
KOiK00611.
OMAiGNNVCNS.
OrthoDBiEOG690MGV.
PhylomeDBiP11724.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. Orn_carbamltrans.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11724-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVRHFLSFM DYSPEELIGL IRRGSELKDL RNRGVLYEPL KSRVLGMVFE
60 70 80 90 100
KASTRTRLSF EAGMIQLGGQ AIFLSPRDTQ LGRGEPIGDS ARVMSRMLDG
110 120 130 140 150
VMIRTFAHAT LTEFAAHSKV PVINGLSDDL HPCQLLADMQ TFHEHRGSIQ
160 170 180 190 200
GKTVAWIGDG NNMCNSYIEA ALKFDFQLRV ACPEGYEPKA EFVALAGDRL
210 220 230 240 250
RVVRDPREAV AGAHLVSTDV WASMGQEDEA AARIALFRPY QVNAALLDGA
260 270 280 290 300
ADDVLFMHCL PAHRGEEISE ELLDDPRSVA WDQAENRLHA QKALLELLIE

HAHYA
Length:305
Mass (Da):33,925
Last modified:January 23, 2007 - v3
Checksum:i6C196BC06BD27F0B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti170 – 1723AAL → EEM in AAA25720 (PubMed:3131308).Curated
Sequence conflicti236 – 2361L → M in AAA25720 (PubMed:3131308).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19939 Genomic DNA. Translation: AAA25720.1.
AE004091 Genomic DNA. Translation: AAG06925.1.
PIRiA32013. OWPSAA.
E83203.
RefSeqiNP_252227.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG06925; AAG06925; PA3537.
GeneIDi878793.
KEGGipae:PA3537.
PATRICi19841687. VBIPseAer58763_3701.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19939 Genomic DNA. Translation: AAA25720.1.
AE004091 Genomic DNA. Translation: AAG06925.1.
PIRiA32013. OWPSAA.
E83203.
RefSeqiNP_252227.1. NC_002516.2.

3D structure databases

ProteinModelPortaliP11724.
SMRiP11724. Positions 4-299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3537.

Protocols and materials databases

DNASUi878793.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06925; AAG06925; PA3537.
GeneIDi878793.
KEGGipae:PA3537.
PATRICi19841687. VBIPseAer58763_3701.

Organism-specific databases

PseudoCAPiPA3537.

Phylogenomic databases

eggNOGiCOG0078.
HOGENOMiHOG000022686.
InParanoidiP11724.
KOiK00611.
OMAiGNNVCNS.
OrthoDBiEOG690MGV.
PhylomeDBiP11724.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.
SABIO-RKP11724.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. Orn_carbamltrans.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Anabolic ornithine carbamoyltransferase of Pseudomonas aeruginosa: nucleotide sequence and transcriptional control of the argF structural gene."
    Itoh Y., Soldati L., Stalon V., Falmagne P., Terawaki Y., Leisinger T., Haas D.
    J. Bacteriol. 170:2725-2734(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-38.
    Strain: PAO.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  3. "The occurrence of a catabolic and an anabolic ornithine carbamoyltransferase in Pseudomonas."
    Stalon V., Ramos F., Pierard A., Wiame J.M.
    Biochim. Biophys. Acta 139:91-97(1967) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN ANABOLIC OTCASE, SUBUNIT.

Entry informationi

Entry nameiOTC_PSEAE
AccessioniPrimary (citable) accession number: P11724
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: February 4, 2015
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.