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Protein

Steroid 17-alpha-hydroxylase/17,20 lyase

Gene

Cyp17a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.

Catalytic activityi

A C(21)-steroid + [reduced NADPH--hemoprotein reductase] + O2 = a 17-alpha-hydroxy-C(21)-steroid + [oxidized NADPH--hemoprotein reductase] + H2O.By similarity
17-alpha-hydroxyprogesterone + [reduced NADPH--hemoprotein reductase] + O2 = androstenedione + acetate + [oxidized NADPH--hemoprotein reductase] + H2O.By similarity
17-alpha-hydroxypregnenolone + [reduced NADPH--hemoprotein reductase] + O2 = 3-beta-hydroxyandrost-5-en-17-one + acetate + [oxidized NADPH--hemoprotein reductase] + H2O.By similarity

Cofactori

hemeBy similarity

Enzyme regulationi

Regulated predominantly by intracellular cAMP levels.

Pathwayi: steroid biosynthesis

This protein is involved in the pathway steroid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway steroid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi441Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

  • adrenal gland development Source: RGD
  • androgen biosynthetic process Source: RGD
  • biphenyl metabolic process Source: RGD
  • cellular response to antibiotic Source: RGD
  • cellular response to gonadotropin stimulus Source: RGD
  • cellular response to lipopolysaccharide Source: RGD
  • dibenzo-p-dioxin metabolic process Source: RGD
  • hippocampus development Source: RGD
  • hormone biosynthetic process Source: UniProtKB
  • Leydig cell differentiation Source: RGD
  • male gonad development Source: RGD
  • organic acid metabolic process Source: RGD
  • ovulation Source: RGD
  • phenol-containing compound metabolic process Source: RGD
  • phthalate metabolic process Source: RGD
  • positive regulation of steroid hormone biosynthetic process Source: RGD
  • progesterone metabolic process Source: UniProtKB
  • response to acetate Source: RGD
  • response to cAMP Source: RGD
  • response to cytokine Source: RGD
  • response to drug Source: RGD
  • response to fungicide Source: RGD
  • response to gonadotropin Source: RGD
  • response to herbicide Source: RGD
  • response to insecticide Source: RGD
  • response to ionizing radiation Source: RGD
  • response to methylmercury Source: RGD
  • response to nutrient levels Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to organic substance Source: RGD
  • response to retinoic acid Source: RGD
  • response to steroid hormone Source: RGD
  • response to toxic substance Source: RGD
  • steroid biosynthetic process Source: RGD
  • steroid metabolic process Source: UniProtKB

Keywordsi

Molecular functionLyase, Monooxygenase, Oxidoreductase
Biological processSteroidogenesis
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00062.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase (EC:1.14.14.19By similarity, EC:1.14.14.32By similarity)
Alternative name(s):
17-alpha-hydroxyprogesterone aldolase
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name:
Cytochrome P450c17
Gene namesi
Name:Cyp17a1
Synonyms:Cyp17
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2456. Cyp17a1.

Subcellular locationi

GO - Cellular componenti

  • cell projection Source: RGD
  • endoplasmic reticulum Source: RGD
  • intracellular membrane-bounded organelle Source: RGD
  • membrane Source: UniProtKB-SubCell
  • neuronal cell body Source: RGD

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4430.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000519411 – 507Steroid 17-alpha-hydroxylase/17,20 lyaseAdd BLAST507

Proteomic databases

PaxDbiP11715.
PRIDEiP11715.

PTM databases

iPTMnetiP11715.
PhosphoSitePlusiP11715.

Interactioni

Protein-protein interaction databases

MINTiMINT-4566010.
STRINGi10116.ENSRNOP00000027160.

Chemistry databases

BindingDBiP11715.

Structurei

3D structure databases

ProteinModelPortaliP11715.
SMRiP11715.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiP11715.
KOiK00512.
PhylomeDBiP11715.
TreeFamiTF105095.

Family and domain databases

InterProiView protein in InterPro
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
PfamiView protein in Pfam
PF00067. p450. 1 hit.
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiView protein in PROSITE
PS00086. CYTOCHROME_P450. 1 hit.

Sequencei

Sequence statusi: Complete.

P11715-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWELVGLLLL ILAYFFWVKS KTPGAKLPRS LPSLPLVGSL PFLPRRGHMH
60 70 80 90 100
VNFFKLQEKY GPIYSLRLGT TTTVIIGHYQ LAREVLIKKG KEFSGRPQMV
110 120 130 140 150
TQSLLSDQGK GVAFADAGSS WHLHRKLVFS TFSLFKDGQK LEKLICQEAK
160 170 180 190 200
SLCDMMLAHD KESIDLSTPI FMSVTNIICA ICFNISYEKN DPKLTAIKTF
210 220 230 240 250
TEGIVDATGD RNLVDIFPWL TIFPNKGLEV IKGYAKVRNE VLTGIFEKCR
260 270 280 290 300
EKFDSQSISS LTDILIQAKM NSDNNNSCEG RDPDVFSDRH ILATVGDIFG
310 320 330 340 350
AGIETTTTVL KWILAFLVHN PEVKKKIQKE IDQYVGFSRT PTFNDRSHLL
360 370 380 390 400
MLEATIREVL RIRPVAPMLI PHKANVDSSI GEFTVPKDTH VVVNLWALHH
410 420 430 440 450
DENEWDQPDQ FMPERFLDPT GSHLITPTQS YLPFGAGPRS CIGEALARQE
460 470 480 490 500
LFVFTALLLQ RFDLDVSDDK QLPRLEGDPK VVFLIDPFKV KITVRQAWMD

AQAEVST
Length:507
Mass (Da):57,250
Last modified:August 1, 1990 - v2
Checksum:iA535600F7E6A399B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti190N → K in AAH78898 (PubMed:15489334).Curated1
Sequence conflicti505 – 506VS → LT in AAA41050 (PubMed:3260774).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14086 mRNA. Translation: CAA32248.1.
X69816 Genomic DNA. Translation: CAA49470.1.
M31681 mRNA. Translation: AAA41777.1.
M22204 mRNA. Translation: AAA41783.1.
BC078898 mRNA. Translation: AAH78898.1.
Z11902 Genomic DNA. Translation: CAA77954.1.
M21208 mRNA. Translation: AAA41050.1.
M27282 mRNA. Translation: AAA41779.1.
PIRiA31359. A30828.
RefSeqiNP_036885.1. NM_012753.2.
XP_006231496.1. XM_006231434.3.
XP_006231497.1. XM_006231435.3.
UniGeneiRn.10172.

Genome annotation databases

GeneIDi25146.
KEGGirno:25146.
UCSCiRGD:2456. rat.

Similar proteinsi

Entry informationi

Entry nameiCP17A_RAT
AccessioniPrimary (citable) accession number: P11715
Secondary accession number(s): Q68FY2, Q6LAE5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: August 1, 1990
Last modified: August 30, 2017
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families