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Reviewed, UniProtKB/Swiss-Prot P11715 (CP17A_RAT)

Last modified October 13, 2009. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Steroid 17-alpha-hydroxylase/17,20 lyase
    EC=1.14.99.9
Alternative name(s):
    Cytochrome P450 17A1
    CYPXVII
    P450-C17
      Short name=P450c17
Gene names
Name: Cyp17a1
Synonyms: Cyp17
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length507 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.

Catalytic activity

A steroid + AH2 + O2 = a 17-alpha-hydroxysteroid + A + H2O.

Cofactor

Heme group By similarity.

Enzyme regulation

Regulated predominantly by intracellular cAMP levels.

Pathway

Lipid metabolism; steroid biosynthesis.

Subcellular location

Membrane Potential. Membrane; Single-pass membrane protein.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 507507Steroid 17-alpha-hydroxylase/17,20 lyase
PRO_0000051941

Sites

Metal binding4411Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict1901N → K in AAH78898. Ref.4
Sequence conflict505 – 5062VS → LT in AAA41050. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P11715-1 [UniParc].

Last modified August 1, 1990. Version 2.
Checksum: A535600F7E6A399B

FASTA50757,250
        10         20         30         40         50         60 
MWELVGLLLL ILAYFFWVKS KTPGAKLPRS LPSLPLVGSL PFLPRRGHMH VNFFKLQEKY 

        70         80         90        100        110        120 
GPIYSLRLGT TTTVIIGHYQ LAREVLIKKG KEFSGRPQMV TQSLLSDQGK GVAFADAGSS 

       130        140        150        160        170        180 
WHLHRKLVFS TFSLFKDGQK LEKLICQEAK SLCDMMLAHD KESIDLSTPI FMSVTNIICA 

       190        200        210        220        230        240 
ICFNISYEKN DPKLTAIKTF TEGIVDATGD RNLVDIFPWL TIFPNKGLEV IKGYAKVRNE 

       250        260        270        280        290        300 
VLTGIFEKCR EKFDSQSISS LTDILIQAKM NSDNNNSCEG RDPDVFSDRH ILATVGDIFG 

       310        320        330        340        350        360 
AGIETTTTVL KWILAFLVHN PEVKKKIQKE IDQYVGFSRT PTFNDRSHLL MLEATIREVL 

       370        380        390        400        410        420 
RIRPVAPMLI PHKANVDSSI GEFTVPKDTH VVVNLWALHH DENEWDQPDQ FMPERFLDPT 

       430        440        450        460        470        480 
GSHLITPTQS YLPFGAGPRS CIGEALARQE LFVFTALLLQ RFDLDVSDDK QLPRLEGDPK 

       490        500 
VVFLIDPFKV KITVRQAWMD AQAEVST 

« Hide

References

« Hide 'large scale' references
[1]"Rat P450(17 alpha) from testis: characterization of a full-length cDNA encoding a unique steroid hydroxylase capable of catalyzing both delta 4- and delta 5-steroid-17,20-lyase reactions."
Fevold H.R., Lorence M.C., McCarthy J.L., Trant J.M., Kagimoto M., Waterman M.R., Mason J.I.
Mol. Endocrinol. 3:968-975(1989) [PubMed: 2786990] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"Rat testis P-450(17)alpha cDNA: the deduced amino acid sequence, expression and secondary structural configuration."
Namiki M., Kitamura M., Buczko E., Dufau M.L.
Biochem. Biophys. Res. Commun. 157:705-712(1988) [PubMed: 3264499] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Testis.
[3]"Transcriptional regulation of rat cytochrome P450c17 expression in mouse Leydig MA-10 and adrenal Y-1 cells: identification of a single protein that mediates both basal and cAMP-induced activities."
Givens C.R., Zhang P., Bair S.R., Mellon S.H.
DNA Cell Biol. 13:1087-1098(1994) [PubMed: 7702752] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sprague-Dawley.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[5]"Gene for 17 alpha-hydroxylase/C (17-20) lyase P-450: complete nucleotide sequence of the porcine gene and 5' upstream sequence of the rat gene."
Zhang P., Nason T.F., Han X.G., Hall P.F.
Biochim. Biophys. Acta 1131:345-348(1992) [PubMed: 1627653] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-97.
[6]"Hormonal regulation of rat Leydig cell cytochrome P-45017 alpha mRNA levels and characterization of a partial length rat P-45017 alpha cDNA."
Nishihara M., Winters C.A., Buzko E., Waterman M.R., Dufau M.L.
Biochem. Biophys. Res. Commun. 154:151-158(1988) [PubMed: 3260774] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 271-507.
[7]"cAMP regulates P450scc gene expression by a cycloheximide-insensitive mechanism in cultured mouse Leydig MA-10 cells."
Mellon S.H., Vaisse C.
Proc. Natl. Acad. Sci. U.S.A. 86:7775-7779(1989) [PubMed: 2554289] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 273-507.
[8]"Purification and characterization of six cytochromes P-450 from hepatic microsomes of immature female rats."
Imaoka S., Kamataki T., Funae Y.
J. Biochem. 102:843-851(1987) [PubMed: 3436956] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-16.
+Additional computationally mapped references.

Cross-references

Sequence databases

X14086 mRNA. Translation: CAA32248.1.
X69816 Genomic DNA. Translation: CAA49470.1.
M31681 mRNA. Translation: AAA41777.1.
M22204 mRNA. Translation: AAA41783.1.
BC078898 mRNA. Translation: AAH78898.1.
Z11902 Genomic DNA. Translation: CAA77954.1.
M21208 mRNA. Translation: AAA41050.1.
M27282 mRNA. Translation: AAA41779.1.
IPIIPI00196701.
PIRA30828. A31359.
RefSeqNP_036885.1.
UniGeneRn.10172

3D structure databases

HSSPHSSP built from PDB template 1DT6 based on UniProtKB P00179.
ModBaseSearch...

Protein-protein interaction databases

STRINGP11715.

PTM databases

PhosphoSiteP11715.

Proteomic databases

PRIDEP11715.

Genome annotation databases

EnsemblENSRNOT00000027160; ENSRNOP00000027160; ENSRNOG00000020035; Rattus norvegicus. [Genome view]
GeneID25146.
KEGGrno:25146.
UCSCNM_012753. rat.

Organism-specific databases

CTD25146.
RGD2456. Cyp17a1.

Phylogenomic databases

HOVERGENP11715.

Enzyme and pathway databases

BRENDA1.14.99.9. 248.

Gene expression databases

ArrayExpressP11715.
GenevestigatorP11715.
GermOnlineENSRNOG00000020035. Rattus norvegicus.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR017973. Cyt_P450_C.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio605573.

Entry information

Entry nameCP17A_RAT
AccessionPrimary (citable) accession number: P11715
Secondary accession number(s): Q68FY2, Q6LAE5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: August 1, 1990
Last modified: October 13, 2009
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents