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P11715

- CP17A_RAT

UniProt

P11715 - CP17A_RAT

Protein

Steroid 17-alpha-hydroxylase/17,20 lyase

Gene

Cyp17a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.

    Catalytic activityi

    A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.
    17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

    Cofactori

    Heme group.By similarity

    Enzyme regulationi

    Regulated predominantly by intracellular cAMP levels.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi441 – 4411Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. 17-alpha-hydroxyprogesterone aldolase activity Source: UniProtKB-EC
    2. heme binding Source: UniProtKB
    3. iron ion binding Source: InterPro
    4. steroid 17-alpha-monooxygenase activity Source: RGD

    GO - Biological processi

    1. adrenal gland development Source: RGD
    2. androgen biosynthetic process Source: RGD
    3. biphenyl metabolic process Source: RGD
    4. cellular response to antibiotic Source: RGD
    5. cellular response to gonadotropin stimulus Source: RGD
    6. cellular response to lipopolysaccharide Source: RGD
    7. dibenzo-p-dioxin metabolic process Source: RGD
    8. glucocorticoid biosynthetic process Source: Ensembl
    9. hippocampus development Source: RGD
    10. hormone biosynthetic process Source: UniProtKB
    11. Leydig cell differentiation Source: RGD
    12. male gonad development Source: RGD
    13. organic acid metabolic process Source: RGD
    14. ovulation Source: RGD
    15. phenol-containing compound metabolic process Source: RGD
    16. phthalate metabolic process Source: RGD
    17. positive regulation of steroid hormone biosynthetic process Source: RGD
    18. progesterone metabolic process Source: UniProtKB
    19. response to acetate Source: RGD
    20. response to cAMP Source: RGD
    21. response to cytokine Source: RGD
    22. response to drug Source: RGD
    23. response to fungicide Source: RGD
    24. response to gonadotropin Source: RGD
    25. response to herbicide Source: RGD
    26. response to insecticide Source: RGD
    27. response to ionizing radiation Source: RGD
    28. response to methylmercury Source: RGD
    29. response to nutrient levels Source: RGD
    30. response to organic cyclic compound Source: RGD
    31. response to organic substance Source: RGD
    32. response to retinoic acid Source: RGD
    33. response to steroid hormone Source: RGD
    34. response to toxic substance Source: RGD
    35. steroid biosynthetic process Source: RGD
    36. steroid metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Lyase, Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Steroidogenesis

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00062.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Steroid 17-alpha-hydroxylase/17,20 lyase (EC:1.14.99.9, EC:4.1.2.30)
    Alternative name(s):
    17-alpha-hydroxyprogesterone aldolase
    CYPXVII
    Cytochrome P450 17A1
    Cytochrome P450-C17
    Short name:
    Cytochrome P450c17
    Gene namesi
    Name:Cyp17a1
    Synonyms:Cyp17
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi2456. Cyp17a1.

    Subcellular locationi

    Membrane Curated

    GO - Cellular componenti

    1. axon Source: Ensembl
    2. cell projection Source: RGD
    3. endoplasmic reticulum Source: RGD
    4. intracellular membrane-bounded organelle Source: RGD
    5. membrane Source: UniProtKB-SubCell
    6. mitochondrion Source: Ensembl
    7. neuronal cell body Source: RGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 507507Steroid 17-alpha-hydroxylase/17,20 lyasePRO_0000051941Add
    BLAST

    Proteomic databases

    PaxDbiP11715.
    PRIDEiP11715.

    PTM databases

    PhosphoSiteiP11715.

    Expressioni

    Gene expression databases

    GenevestigatoriP11715.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4566010.

    Structurei

    3D structure databases

    ProteinModelPortaliP11715.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00750000117317.
    HOGENOMiHOG000036991.
    HOVERGENiHBG106944.
    InParanoidiP11715.
    KOiK00512.
    OrthoDBiEOG7RBZ85.
    PhylomeDBiP11715.
    TreeFamiTF105095.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P11715-1 [UniParc]FASTAAdd to Basket

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    MWELVGLLLL ILAYFFWVKS KTPGAKLPRS LPSLPLVGSL PFLPRRGHMH    50
    VNFFKLQEKY GPIYSLRLGT TTTVIIGHYQ LAREVLIKKG KEFSGRPQMV 100
    TQSLLSDQGK GVAFADAGSS WHLHRKLVFS TFSLFKDGQK LEKLICQEAK 150
    SLCDMMLAHD KESIDLSTPI FMSVTNIICA ICFNISYEKN DPKLTAIKTF 200
    TEGIVDATGD RNLVDIFPWL TIFPNKGLEV IKGYAKVRNE VLTGIFEKCR 250
    EKFDSQSISS LTDILIQAKM NSDNNNSCEG RDPDVFSDRH ILATVGDIFG 300
    AGIETTTTVL KWILAFLVHN PEVKKKIQKE IDQYVGFSRT PTFNDRSHLL 350
    MLEATIREVL RIRPVAPMLI PHKANVDSSI GEFTVPKDTH VVVNLWALHH 400
    DENEWDQPDQ FMPERFLDPT GSHLITPTQS YLPFGAGPRS CIGEALARQE 450
    LFVFTALLLQ RFDLDVSDDK QLPRLEGDPK VVFLIDPFKV KITVRQAWMD 500
    AQAEVST 507
    Length:507
    Mass (Da):57,250
    Last modified:August 1, 1990 - v2
    Checksum:iA535600F7E6A399B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti190 – 1901N → K in AAH78898. (PubMed:15489334)Curated
    Sequence conflicti505 – 5062VS → LT in AAA41050. (PubMed:3260774)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14086 mRNA. Translation: CAA32248.1.
    X69816 Genomic DNA. Translation: CAA49470.1.
    M31681 mRNA. Translation: AAA41777.1.
    M22204 mRNA. Translation: AAA41783.1.
    BC078898 mRNA. Translation: AAH78898.1.
    Z11902 Genomic DNA. Translation: CAA77954.1.
    M21208 mRNA. Translation: AAA41050.1.
    M27282 mRNA. Translation: AAA41779.1.
    PIRiA31359. A30828.
    RefSeqiNP_036885.1. NM_012753.2.
    XP_006231496.1. XM_006231434.1.
    XP_006231497.1. XM_006231435.1.
    UniGeneiRn.10172.

    Genome annotation databases

    EnsembliENSRNOT00000027160; ENSRNOP00000027160; ENSRNOG00000020035.
    GeneIDi25146.
    KEGGirno:25146.
    UCSCiRGD:2456. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14086 mRNA. Translation: CAA32248.1 .
    X69816 Genomic DNA. Translation: CAA49470.1 .
    M31681 mRNA. Translation: AAA41777.1 .
    M22204 mRNA. Translation: AAA41783.1 .
    BC078898 mRNA. Translation: AAH78898.1 .
    Z11902 Genomic DNA. Translation: CAA77954.1 .
    M21208 mRNA. Translation: AAA41050.1 .
    M27282 mRNA. Translation: AAA41779.1 .
    PIRi A31359. A30828.
    RefSeqi NP_036885.1. NM_012753.2.
    XP_006231496.1. XM_006231434.1.
    XP_006231497.1. XM_006231435.1.
    UniGenei Rn.10172.

    3D structure databases

    ProteinModelPortali P11715.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4566010.

    Chemistry

    BindingDBi P11715.
    ChEMBLi CHEMBL4430.

    PTM databases

    PhosphoSitei P11715.

    Proteomic databases

    PaxDbi P11715.
    PRIDEi P11715.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000027160 ; ENSRNOP00000027160 ; ENSRNOG00000020035 .
    GeneIDi 25146.
    KEGGi rno:25146.
    UCSCi RGD:2456. rat.

    Organism-specific databases

    CTDi 1586.
    RGDi 2456. Cyp17a1.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00750000117317.
    HOGENOMi HOG000036991.
    HOVERGENi HBG106944.
    InParanoidi P11715.
    KOi K00512.
    OrthoDBi EOG7RBZ85.
    PhylomeDBi P11715.
    TreeFami TF105095.

    Enzyme and pathway databases

    UniPathwayi UPA00062 .

    Miscellaneous databases

    NextBioi 605573.
    PROi P11715.

    Gene expression databases

    Genevestigatori P11715.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rat P450(17 alpha) from testis: characterization of a full-length cDNA encoding a unique steroid hydroxylase capable of catalyzing both delta 4- and delta 5-steroid-17,20-lyase reactions."
      Fevold H.R., Lorence M.C., McCarthy J.L., Trant J.M., Kagimoto M., Waterman M.R., Mason J.I.
      Mol. Endocrinol. 3:968-975(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "Rat testis P-450(17)alpha cDNA: the deduced amino acid sequence, expression and secondary structural configuration."
      Namiki M., Kitamura M., Buczko E., Dufau M.L.
      Biochem. Biophys. Res. Commun. 157:705-712(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Tissue: Testis.
    3. "Transcriptional regulation of rat cytochrome P450c17 expression in mouse Leydig MA-10 and adrenal Y-1 cells: identification of a single protein that mediates both basal and cAMP-induced activities."
      Givens C.R., Zhang P., Bair S.R., Mellon S.H.
      DNA Cell Biol. 13:1087-1098(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    5. "Gene for 17 alpha-hydroxylase/C (17-20) lyase P-450: complete nucleotide sequence of the porcine gene and 5' upstream sequence of the rat gene."
      Zhang P., Nason T.F., Han X.G., Hall P.F.
      Biochim. Biophys. Acta 1131:345-348(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-97.
    6. "Hormonal regulation of rat Leydig cell cytochrome P-45017 alpha mRNA levels and characterization of a partial length rat P-45017 alpha cDNA."
      Nishihara M., Winters C.A., Buzko E., Waterman M.R., Dufau M.L.
      Biochem. Biophys. Res. Commun. 154:151-158(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 271-507.
    7. "cAMP regulates P450scc gene expression by a cycloheximide-insensitive mechanism in cultured mouse Leydig MA-10 cells."
      Mellon S.H., Vaisse C.
      Proc. Natl. Acad. Sci. U.S.A. 86:7775-7779(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 273-507.
    8. "Purification and characterization of six cytochromes P-450 from hepatic microsomes of immature female rats."
      Imaoka S., Kamataki T., Funae Y.
      J. Biochem. 102:843-851(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-16.

    Entry informationi

    Entry nameiCP17A_RAT
    AccessioniPrimary (citable) accession number: P11715
    Secondary accession number(s): Q68FY2, Q6LAE5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3