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Protein

Cytochrome P450 2D9

Gene

Cyp2d9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi446 – 4461Iron (heme axial ligand)

GO - Molecular functioni

  1. arachidonic acid epoxygenase activity Source: GO_Central
  2. aromatase activity Source: UniProtKB-EC
  3. heme binding Source: GO_Central
  4. iron ion binding Source: InterPro
  5. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
  6. oxygen binding Source: GO_Central
  7. steroid hydroxylase activity Source: GO_Central

GO - Biological processi

  1. arachidonic acid metabolic process Source: GO_Central
  2. exogenous drug catabolic process Source: GO_Central
  3. oxidation-reduction process Source: GO_Central
  4. xenobiotic metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_245291. Xenobiotics.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2D9 (EC:1.14.14.1)
Alternative name(s):
CYPIID9
Cytochrome P450-16-alpha
Cytochrome P450CA
Testosterone 16-alpha hydroxylase
Gene namesi
Name:Cyp2d9
Synonyms:Cyp2d-9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:88606. Cyp2d9.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. intracellular membrane-bounded organelle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Cytochrome P450 2D9PRO_0000051734Add
BLAST

Proteomic databases

PaxDbiP11714.
PRIDEiP11714.

Expressioni

Inductioni

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Gene expression databases

BgeeiP11714.
GenevestigatoriP11714.

Interactioni

Protein-protein interaction databases

IntActiP11714. 1 interaction.
MINTiMINT-4122639.

Structurei

3D structure databases

ProteinModelPortaliP11714.
SMRiP11714. Positions 51-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036991.
HOVERGENiHBG015789.
InParanoidiP11714.
KOiK07414.
OrthoDBiEOG7RBZ85.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01686. EP450ICYP2D.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11714-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLTGTDLW PVAIFTVIFI LLVDLTHQRQ RWTSRYPPGP VPWPVLGNLL
60 70 80 90 100
QVDLGNMPYS LYKLQNRYGD VFSLQMAWKP MVVINGLKAM KEMLLTCGED
110 120 130 140 150
TADRPPVPIF EYLGVKPGSQ GVVLAPYGPE WREQRRFSVS TLRNFGLGKK
160 170 180 190 200
SLEDWVTKEA NHLCDAFTAQ AGQPINPNPM LNKSTCNVIA SLIFARRFEY
210 220 230 240 250
EDPFLIRMLK VLEQSLTEVS GLIPEVLNAF PILLRIPRLA DKALQGQKSF
260 270 280 290 300
IAILDNLLTE NRTTWDPVQA PRNLTDAFLA EIEKAKGNPE SSFNDENLLM
310 320 330 340 350
VVRDLFGAGM LTTSTTLSWA LMLMILHPDV QRRVQQEIDE VIGQVRHPEM
360 370 380 390 400
ADQAHMPYTN AVIHEVQRFG DIVPVNLPRI TSHDIEVQDF LIPKGTILLP
410 420 430 440 450
NMSSMLKDES VWEKPLRFHP EHFLDAQGHF VKPEAFMPFS AGRRSCLGEA
460 470 480 490 500
LARMELFLFF TCLLQRFSFS VPDGQPQPSN SGVYGILVAP SPYQLCAVVR

DQGH
Length:504
Mass (Da):56,950
Last modified:July 27, 2011 - v2
Checksum:i6DC93B3985EFB8A2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 552LG → QD in AAA39876 (PubMed:2788458).Curated
Sequence conflicti281 – 2811E → Q in AAA40428 (PubMed:2831949).Curated
Sequence conflicti281 – 2811E → Q in AAA40427 (PubMed:2831949).Curated
Sequence conflicti281 – 2811E → Q in AAA39876 (PubMed:2788458).Curated
Sequence conflicti281 – 2811E → Q in AAA75462 (PubMed:2914938).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23998 mRNA. Translation: AAA40427.1.
M23997 mRNA. Translation: AAA40428.1. Sequence problems.
M27168 mRNA. Translation: AAA39876.1.
M24267, M24262 Genomic DNA. Translation: AAA75462.1.
CH466550 Genomic DNA. Translation: EDL04501.1.
BC010593 mRNA. Translation: AAH10593.1.
BC094015 mRNA. Translation: AAH94015.1.
CCDSiCCDS27691.1.
PIRiB27384.
S15806. A27384.
RefSeqiNP_034136.2. NM_010006.2.
UniGeneiMm.226708.

Genome annotation databases

EnsembliENSMUST00000089129; ENSMUSP00000086530; ENSMUSG00000068086.
GeneIDi13105.
KEGGimmu:13105.
UCSCiuc007wzg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23998 mRNA. Translation: AAA40427.1.
M23997 mRNA. Translation: AAA40428.1. Sequence problems.
M27168 mRNA. Translation: AAA39876.1.
M24267, M24262 Genomic DNA. Translation: AAA75462.1.
CH466550 Genomic DNA. Translation: EDL04501.1.
BC010593 mRNA. Translation: AAH10593.1.
BC094015 mRNA. Translation: AAH94015.1.
CCDSiCCDS27691.1.
PIRiB27384.
S15806. A27384.
RefSeqiNP_034136.2. NM_010006.2.
UniGeneiMm.226708.

3D structure databases

ProteinModelPortaliP11714.
SMRiP11714. Positions 51-500.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP11714. 1 interaction.
MINTiMINT-4122639.

Proteomic databases

PaxDbiP11714.
PRIDEiP11714.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000089129; ENSMUSP00000086530; ENSMUSG00000068086.
GeneIDi13105.
KEGGimmu:13105.
UCSCiuc007wzg.2. mouse.

Organism-specific databases

CTDi13105.
MGIiMGI:88606. Cyp2d9.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036991.
HOVERGENiHBG015789.
InParanoidiP11714.
KOiK07414.
OrthoDBiEOG7RBZ85.
TreeFamiTF352043.

Enzyme and pathway databases

ReactomeiREACT_245291. Xenobiotics.

Miscellaneous databases

NextBioi283098.
PROiP11714.
SOURCEiSearch...

Gene expression databases

BgeeiP11714.
GenevestigatoriP11714.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01686. EP450ICYP2D.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gene family of male-specific testosterone 16 alpha-hydroxylase (C-P-450(16) alpha) in mouse liver: cDNA sequences, neonatal imprinting, and reversible regulation by androgen."
    Wong G., Kawajiri K., Negishi M.
    Biochemistry 26:8683-8690(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Functional characterization of two cytochrome P-450s within the mouse, male-specific steroid 16 alpha-hydroxylase gene family: expression in mammalian cells and chimeric proteins."
    Ichikawa T., Itakura T., Negishi M.
    Biochemistry 28:4779-4784(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129/J.
    Tissue: Liver.
  3. "Gene family of male-specific testosterone 16 alpha-hydroxylase (C-P-450(16 alpha)) in mice. Organization, differential regulation, and chromosome localization."
    Wong G., Itakura T., Kawajiri K., Skow L., Negishi M.
    J. Biol. Chem. 264:2920-2927(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiCP2D9_MOUSE
AccessioniPrimary (citable) accession number: P11714
Secondary accession number(s): Q64489, Q921V1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 27, 2011
Last modified: February 4, 2015
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.