Reviewed, UniProtKB/Swiss-Prot P11714 (CP2D9_MOUSE)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 2D9
    EC=1.14.14.1
Alternative name(s):
    CYPIID9
    P450-16-alpha
    CA
    Testosterone 16-alpha hydroxylase

Gene names

Name:	Cyp2d9
Synonyms:	Cyp2d-9

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from direct assay. Source: MGI
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Cytochrome P450 2D9		PRO_0000051734
Sites
	Metal binding	446	1	Iron (heme axial ligand)
Amino acid modifications
	Modified residue	138	1	Phosphoserine Ref.4
Experimental info
	Sequence conflict	54 – 55	2	LG → QD in AAA39876. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P11714-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: B85862205472A176	FASTA	504	56,949
	10 20 30 40 50 60 MELLTGTDLW PVAIFTVIFI LLVDLTHQRQ RWTSRYPPGP VPWPVLGNLL QVDLGNMPYS 70 80 90 100 110 120 LYKLQNRYGD VFSLQMAWKP MVVINGLKAM KEMLLTCGED TADRPPVPIF EYLGVKPGSQ 130 140 150 160 170 180 GVVLAPYGPE WREQRRFSVS TLRNFGLGKK SLEDWVTKEA NHLCDAFTAQ AGQPINPNPM 190 200 210 220 230 240 LNKSTCNVIA SLIFARRFEY EDPFLIRMLK VLEQSLTEVS GLIPEVLNAF PILLRIPRLA 250 260 270 280 290 300 DKALQGQKSF IAILDNLLTE NRTTWDPVQA PRNLTDAFLA QIEKAKGNPE SSFNDENLLM 310 320 330 340 350 360 VVRDLFGAGM LTTSTTLSWA LMLMILHPDV QRRVQQEIDE VIGQVRHPEM ADQAHMPYTN 370 380 390 400 410 420 AVIHEVQRFG DIVPVNLPRI TSHDIEVQDF LIPKGTILLP NMSSMLKDES VWEKPLRFHP 430 440 450 460 470 480 EHFLDAQGHF VKPEAFMPFS AGRRSCLGEA LARMELFLFF TCLLQRFSFS VPDGQPQPSN 490 500 SGVYGILVAP SPYQLCAVVR DQGH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene family of male-specific testosterone 16 alpha-hydroxylase (C-P-450(16) alpha) in mouse liver: cDNA sequences, neonatal imprinting, and reversible regulation by androgen." Wong G., Kawajiri K., Negishi M. Biochemistry 26:8683-8690(1987) [PubMed: 2831949] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Functional characterization of two cytochrome P-450s within the mouse, male-specific steroid 16 alpha-hydroxylase gene family: expression in mammalian cells and chimeric proteins." Ichikawa T., Itakura T., Negishi M. Biochemistry 28:4779-4784(1989) [PubMed: 2788458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129/J. Tissue: Liver.
[3]	"Gene family of male-specific testosterone 16 alpha-hydroxylase (C-P-450(16 alpha)) in mice. Organization, differential regulation, and chromosome localization." Wong G., Itakura T., Kawajiri K., Skow L., Negishi M. J. Biol. Chem. 264:2920-2927(1989) [PubMed: 2914938] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[4]	"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis." Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H. J. Proteome Res. 7:3957-3967(2008) [PubMed: 18630941] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-138, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M23998 mRNA. Translation: AAA40427.1. M23997 mRNA. Translation: AAA40428.1. Sequence problems. M27168 mRNA. Translation: AAA39876.1. M24267, M24262 Genomic DNA. Translation: AAA75462.1.
IPI	IPI00116572.
PIR	B27384. A27384. S15806.
UniGene	Mm.226708
3D structure databases
HSSP	HSSP built from PDB template 1DT6 based on UniProtKB P00179.
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000068086. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:88606. Cyp2d9.
Phylogenomic databases
HOGENOM	P11714.
HOVERGEN	P11714.
Enzyme and pathway databases
BRENDA	1.14.14.1. 244.
Gene expression databases
ArrayExpress	P11714.
Bgee	P11714.
GermOnline	ENSMUSG00000068086. Mus musculus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008069. Cyt_P450_E_grp-I_CYP2D-like. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01686. EP450ICYP2D. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

CP2D9_MOUSE

Accession

Primary (citable) accession number: P11714
Secondary accession number(s): Q64489

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	April 14, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)