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P11711

- CP2A1_RAT

UniProt

P11711 - CP2A1_RAT

Protein

Cytochrome P450 2A1

Gene

Cyp2a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (01 May 2007)
      Previous versions | rss
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    Functioni

    Highly active in the 7-alpha-hydroxylation of testosterone, progesterone and androstenedione.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi437 – 4371Iron (heme axial ligand)

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. oxidoreductase activity Source: RGD
    5. steroid hydroxylase activity Source: RGD

    GO - Biological processi

    1. coumarin metabolic process Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2A1 (EC:1.14.14.1)
    Alternative name(s):
    CYPIIA1
    Cytochrome P450-UT-F
    Steroid hormones 7-alpha-hydroxylase
    Testosterone 7-alpha-hydroxylase
    Gene namesi
    Name:Cyp2a1
    Synonyms:Cyp2a-1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi2463. Cyp2a1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. intracellular membrane-bounded organelle Source: RGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 492492Cytochrome P450 2A1PRO_0000051663Add
    BLAST

    Proteomic databases

    PaxDbiP11711.
    PRIDEiP11711.

    Expressioni

    Tissue specificityi

    Liver and testis.

    Inductioni

    By 3-methylcholanthrene (3MC).

    Gene expression databases

    GenevestigatoriP11711.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4576797.
    STRINGi10116.ENSRNOP00000028237.

    Structurei

    3D structure databases

    ProteinModelPortaliP11711.
    SMRiP11711. Positions 30-491.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00670000097868.
    HOGENOMiHOG000036992.
    HOVERGENiHBG015789.
    InParanoidiP11711.
    KOiK07411.
    OMAiGSLMTDP.
    OrthoDBiEOG7RBZ85.
    PhylomeDBiP11711.
    TreeFamiTF352043.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01684. EP450ICYP2A.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P11711-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLDTGLLLVV ILASLSVMLL VSLWQQKIRG RLPPGPTPLP FIGNYLQLNT    50
    KDVYSSITQL SERYGPVFTI HLGPRRVVVL YGYDAVKEAL VDQAEEFSGR 100
    GEQATYNTLF KGYGVAFSSG ERAKQLRRLS IATLRDFGVG KRGVEERILE 150
    EAGYLIKMLQ GTCGAPIDPT IYLSKTVSNV ISSIVFGERF DYEDTEFLSL 200
    LQMMGQMNRF AASPTGQLYD MFHSVMKYLP GPQQQIIKVT QKLEDFMIEK 250
    VRQNHSTLDP NSPRNFIDSF LIRMQEEKNG NSEFHMKNLV MTTLSLFFAG 300
    SETVSSTLRY GFLLLMKHPD VEAKVHEEIE QVIGRNRQPQ YEDHMKMPYT 350
    QAVINEIQRF SNLAPLGIPR RIIKNTTFRG FFLPKGTDVF PILGSLMTDP 400
    KFFPSPKDFD PQNFLDDKGQ LKKNAAFLPF STGKRFCLGD GLAKMELFLL 450
    LTTILQNFRF KFPMKLEDIN ESPKPLGFTR IIPKYTMSFM PI 492
    Length:492
    Mass (Da):55,995
    Last modified:May 1, 2007 - v2
    Checksum:i58A187F9F10A1D85
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti386 – 3861G → A in AAA41030. (PubMed:3818621)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02669 mRNA. Translation: AAA41030.1.
    M33312 Genomic DNA. Translation: AAA41020.1.
    BC081848 mRNA. Translation: AAH81848.1.
    PIRiA34272.
    RefSeqiNP_036824.1. NM_012692.1.
    UniGeneiRn.32107.

    Genome annotation databases

    EnsembliENSRNOT00000028237; ENSRNOP00000028237; ENSRNOG00000020928.
    GeneIDi24894.
    KEGGirno:24894.
    UCSCiRGD:2463. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02669 mRNA. Translation: AAA41030.1 .
    M33312 Genomic DNA. Translation: AAA41020.1 .
    BC081848 mRNA. Translation: AAH81848.1 .
    PIRi A34272.
    RefSeqi NP_036824.1. NM_012692.1.
    UniGenei Rn.32107.

    3D structure databases

    ProteinModelPortali P11711.
    SMRi P11711. Positions 30-491.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4576797.
    STRINGi 10116.ENSRNOP00000028237.

    Proteomic databases

    PaxDbi P11711.
    PRIDEi P11711.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000028237 ; ENSRNOP00000028237 ; ENSRNOG00000020928 .
    GeneIDi 24894.
    KEGGi rno:24894.
    UCSCi RGD:2463. rat.

    Organism-specific databases

    CTDi 24894.
    RGDi 2463. Cyp2a1.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00670000097868.
    HOGENOMi HOG000036992.
    HOVERGENi HBG015789.
    InParanoidi P11711.
    KOi K07411.
    OMAi GSLMTDP.
    OrthoDBi EOG7RBZ85.
    PhylomeDBi P11711.
    TreeFami TF352043.

    Miscellaneous databases

    NextBioi 604766.
    PROi P11711.

    Gene expression databases

    Genevestigatori P11711.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01684. EP450ICYP2A.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rat testosterone 7 alpha-hydroxylase. Isolation, sequence, and expression of cDNA and its developmental regulation and induction by 3-methylcholanthrene."
      Nagata K., Matsunaga T., Gillette J., Gelboin H.V., Gonzalez F.J.
      J. Biol. Chem. 262:2787-2793(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "Structure and in vitro transcription of the rat CYP2A1 and CYP2A2 genes and regional localization of the CYP2A gene subfamily on mouse chromosome 7."
      Matsunaga T., Nomoto M., Kozak C.A., Gonzalez F.J.
      Biochemistry 29:1329-1341(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    4. "Purification of two isozymes of rat liver microsomal cytochrome P450 with testosterone 7 alpha-hydroxylase activity."
      Arlotto M.P., Greenway D.J., Parkinson A.
      Arch. Biochem. Biophys. 270:441-457(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-20.
      Tissue: Liver.
    5. "Purification and characterization of six cytochromes P-450 from hepatic microsomes of immature female rats."
      Imaoka S., Kamataki T., Funae Y.
      J. Biochem. 102:843-851(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-12.
      Tissue: Liver.

    Entry informationi

    Entry nameiCP2A1_RAT
    AccessioniPrimary (citable) accession number: P11711
    Secondary accession number(s): Q642C5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3