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Protein

Cytochrome P450 2A1

Gene

Cyp2a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Highly active in the 7-alpha-hydroxylation of testosterone, progesterone and androstenedione.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi437 – 4371Iron (heme axial ligand)

GO - Molecular functioni

  1. arachidonic acid epoxygenase activity Source: GO_Central
  2. aromatase activity Source: UniProtKB-EC
  3. heme binding Source: GO_Central
  4. iron ion binding Source: InterPro
  5. oxidoreductase activity Source: RGD
  6. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
  7. oxygen binding Source: GO_Central
  8. steroid hydroxylase activity Source: RGD

GO - Biological processi

  1. coumarin metabolic process Source: RGD
  2. epoxygenase P450 pathway Source: GO_Central
  3. exogenous drug catabolic process Source: GO_Central
  4. oxidation-reduction process Source: GO_Central
  5. xenobiotic metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_278052. Aflatoxin activation and detoxification.
REACT_315621. Xenobiotics.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2A1 (EC:1.14.14.1)
Alternative name(s):
CYPIIA1
Cytochrome P450-UT-F
Steroid hormones 7-alpha-hydroxylase
Testosterone 7-alpha-hydroxylase
Gene namesi
Name:Cyp2a1
Synonyms:Cyp2a-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi2463. Cyp2a1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Chemistry

DrugBankiDB08834. Tauroursodeoxycholic acid.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 492492Cytochrome P450 2A1PRO_0000051663Add
BLAST

Proteomic databases

PaxDbiP11711.
PRIDEiP11711.

Expressioni

Tissue specificityi

Liver and testis.

Inductioni

By 3-methylcholanthrene (3MC).

Gene expression databases

GenevestigatoriP11711.

Interactioni

Protein-protein interaction databases

MINTiMINT-4576797.
STRINGi10116.ENSRNOP00000028237.

Structurei

3D structure databases

SMRiP11711. Positions 30-491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP11711.
KOiK07411.
OMAiGSLMTDP.
OrthoDBiEOG7RBZ85.
PhylomeDBiP11711.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11711-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDTGLLLVV ILASLSVMLL VSLWQQKIRG RLPPGPTPLP FIGNYLQLNT
60 70 80 90 100
KDVYSSITQL SERYGPVFTI HLGPRRVVVL YGYDAVKEAL VDQAEEFSGR
110 120 130 140 150
GEQATYNTLF KGYGVAFSSG ERAKQLRRLS IATLRDFGVG KRGVEERILE
160 170 180 190 200
EAGYLIKMLQ GTCGAPIDPT IYLSKTVSNV ISSIVFGERF DYEDTEFLSL
210 220 230 240 250
LQMMGQMNRF AASPTGQLYD MFHSVMKYLP GPQQQIIKVT QKLEDFMIEK
260 270 280 290 300
VRQNHSTLDP NSPRNFIDSF LIRMQEEKNG NSEFHMKNLV MTTLSLFFAG
310 320 330 340 350
SETVSSTLRY GFLLLMKHPD VEAKVHEEIE QVIGRNRQPQ YEDHMKMPYT
360 370 380 390 400
QAVINEIQRF SNLAPLGIPR RIIKNTTFRG FFLPKGTDVF PILGSLMTDP
410 420 430 440 450
KFFPSPKDFD PQNFLDDKGQ LKKNAAFLPF STGKRFCLGD GLAKMELFLL
460 470 480 490
LTTILQNFRF KFPMKLEDIN ESPKPLGFTR IIPKYTMSFM PI
Length:492
Mass (Da):55,995
Last modified:May 1, 2007 - v2
Checksum:i58A187F9F10A1D85
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti386 – 3861G → A in AAA41030 (PubMed:3818621).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02669 mRNA. Translation: AAA41030.1.
M33312 Genomic DNA. Translation: AAA41020.1.
BC081848 mRNA. Translation: AAH81848.1.
PIRiA34272.
RefSeqiNP_036824.1. NM_012692.1.
XP_008757327.1. XM_008759105.1.
XP_008757328.1. XM_008759106.1.
UniGeneiRn.32107.

Genome annotation databases

EnsembliENSRNOT00000028237; ENSRNOP00000028237; ENSRNOG00000020928.
GeneIDi24894.
KEGGirno:24894.
UCSCiRGD:2463. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02669 mRNA. Translation: AAA41030.1.
M33312 Genomic DNA. Translation: AAA41020.1.
BC081848 mRNA. Translation: AAH81848.1.
PIRiA34272.
RefSeqiNP_036824.1. NM_012692.1.
XP_008757327.1. XM_008759105.1.
XP_008757328.1. XM_008759106.1.
UniGeneiRn.32107.

3D structure databases

SMRiP11711. Positions 30-491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4576797.
STRINGi10116.ENSRNOP00000028237.

Chemistry

DrugBankiDB08834. Tauroursodeoxycholic acid.

Proteomic databases

PaxDbiP11711.
PRIDEiP11711.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028237; ENSRNOP00000028237; ENSRNOG00000020928.
GeneIDi24894.
KEGGirno:24894.
UCSCiRGD:2463. rat.

Organism-specific databases

CTDi24894.
RGDi2463. Cyp2a1.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP11711.
KOiK07411.
OMAiGSLMTDP.
OrthoDBiEOG7RBZ85.
PhylomeDBiP11711.
TreeFamiTF352043.

Enzyme and pathway databases

ReactomeiREACT_278052. Aflatoxin activation and detoxification.
REACT_315621. Xenobiotics.

Miscellaneous databases

NextBioi604766.
PROiP11711.

Gene expression databases

GenevestigatoriP11711.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rat testosterone 7 alpha-hydroxylase. Isolation, sequence, and expression of cDNA and its developmental regulation and induction by 3-methylcholanthrene."
    Nagata K., Matsunaga T., Gillette J., Gelboin H.V., Gonzalez F.J.
    J. Biol. Chem. 262:2787-2793(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Structure and in vitro transcription of the rat CYP2A1 and CYP2A2 genes and regional localization of the CYP2A gene subfamily on mouse chromosome 7."
    Matsunaga T., Nomoto M., Kozak C.A., Gonzalez F.J.
    Biochemistry 29:1329-1341(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. "Purification of two isozymes of rat liver microsomal cytochrome P450 with testosterone 7 alpha-hydroxylase activity."
    Arlotto M.P., Greenway D.J., Parkinson A.
    Arch. Biochem. Biophys. 270:441-457(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-20.
    Tissue: Liver.
  5. "Purification and characterization of six cytochromes P-450 from hepatic microsomes of immature female rats."
    Imaoka S., Kamataki T., Funae Y.
    J. Biochem. 102:843-851(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-12.
    Tissue: Liver.

Entry informationi

Entry nameiCP2A1_RAT
AccessioniPrimary (citable) accession number: P11711
Secondary accession number(s): Q642C5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: May 1, 2007
Last modified: April 1, 2015
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.