Reviewed, UniProtKB/Swiss-Prot P11711 (CP2A1_RAT)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 2A1
    EC=1.14.14.1
Alternative name(s):
    CYPIIA1
    Steroid hormones 7-alpha-hydroxylase
    Testosterone 7-alpha-hydroxylase
    P450-UT-F

Gene names

Name:	Cyp2a1
Synonyms:	Cyp2a-1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Highly active in the 7-alpha-hydroxylation of testosterone, progesterone and androstenedione.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Liver and testis.
Induction	By 3-methylcholanthrene (3MC).
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	coumarin metabolic process Inferred from direct assay. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Ref.1 Inferred from direct assay. Source: RGD
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro steroid hydroxylase activity Ref.1 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Cytochrome P450 2A1		PRO_0000051663
Sites
	Metal binding	437	1	Iron (heme axial ligand)
Experimental info
	Sequence conflict	386	1	G → A in AAA41030. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P11711-1 [UniParc]. Last modified May 1, 2007. Version 2. Checksum: 58A187F9F10A1D85	FASTA	492	55,995
	10 20 30 40 50 60 MLDTGLLLVV ILASLSVMLL VSLWQQKIRG RLPPGPTPLP FIGNYLQLNT KDVYSSITQL 70 80 90 100 110 120 SERYGPVFTI HLGPRRVVVL YGYDAVKEAL VDQAEEFSGR GEQATYNTLF KGYGVAFSSG 130 140 150 160 170 180 ERAKQLRRLS IATLRDFGVG KRGVEERILE EAGYLIKMLQ GTCGAPIDPT IYLSKTVSNV 190 200 210 220 230 240 ISSIVFGERF DYEDTEFLSL LQMMGQMNRF AASPTGQLYD MFHSVMKYLP GPQQQIIKVT 250 260 270 280 290 300 QKLEDFMIEK VRQNHSTLDP NSPRNFIDSF LIRMQEEKNG NSEFHMKNLV MTTLSLFFAG 310 320 330 340 350 360 SETVSSTLRY GFLLLMKHPD VEAKVHEEIE QVIGRNRQPQ YEDHMKMPYT QAVINEIQRF 370 380 390 400 410 420 SNLAPLGIPR RIIKNTTFRG FFLPKGTDVF PILGSLMTDP KFFPSPKDFD PQNFLDDKGQ 430 440 450 460 470 480 LKKNAAFLPF STGKRFCLGD GLAKMELFLL LTTILQNFRF KFPMKLEDIN ESPKPLGFTR 490 IIPKYTMSFM PI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rat testosterone 7 alpha-hydroxylase. Isolation, sequence, and expression of cDNA and its developmental regulation and induction by 3-methylcholanthrene." Nagata K., Matsunaga T., Gillette J., Gelboin H.V., Gonzalez F.J. J. Biol. Chem. 262:2787-2793(1987) [PubMed: 3818621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Structure and in vitro transcription of the rat CYP2A1 and CYP2A2 genes and regional localization of the CYP2A gene subfamily on mouse chromosome 7." Matsunaga T., Nomoto M., Kozak C.A., Gonzalez F.J. Biochemistry 29:1329-1341(1990) [PubMed: 2322568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	"Purification of two isozymes of rat liver microsomal cytochrome P450 with testosterone 7 alpha-hydroxylase activity." Arlotto M.P., Greenway D.J., Parkinson A. Arch. Biochem. Biophys. 270:441-457(1989) [PubMed: 2650624] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-20. Tissue: Liver.
[5]	"Purification and characterization of six cytochromes P-450 from hepatic microsomes of immature female rats." Imaoka S., Kamataki T., Funae Y. J. Biochem. 102:843-851(1987) [PubMed: 3436956] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J02669 mRNA. Translation: AAA41030.1. M33312 Genomic DNA. Translation: AAA41020.1. BC081848 mRNA. Translation: AAH81848.1.
IPI	IPI00196696.
PIR	A34272.
RefSeq	NP_036824.1.
UniGene	Rn.32107
3D structure databases
HSSP	HSSP built from PDB template 1DT6 based on UniProtKB P00179.
ModBase	Search...
Protein-protein interaction databases
STRING	P11711.
Proteomic databases
PRIDE	P11711.
Genome annotation databases
Ensembl	ENSRNOT00000028237; ENSRNOP00000028237; ENSRNOG00000020928; Rattus norvegicus. [Genome view]
GeneID	24894.
KEGG	rno:24894.
UCSC	NM_012692. rat.
Organism-specific databases
CTD	24894.
RGD	2463. Cyp2a1.
Phylogenomic databases
HOVERGEN	P11711.
OMA	EDINESP.
Enzyme and pathway databases
BRENDA	1.14.14.1. 248.
Gene expression databases
ArrayExpress	P11711.
Genevestigator	P11711.
GermOnline	ENSRNOG00000020928. Rattus norvegicus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008067. Cyt_P450_E_grp-I_CYP2A-like. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01684. EP450ICYP2A. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	604766.

Entry information

Entry name

CP2A1_RAT

Accession

Primary (citable) accession number: P11711
Secondary accession number(s): Q642C5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	May 1, 2007
Last modified:	November 3, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)