Cytochrome P450 2A1 - Rattus norvegicus (Rat)

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P11711 (CP2A1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cytochrome P450 2A1
EC=1.14.14.1

Alternative name(s):
CYPIIA1
Cytochrome P450-UT-F
Steroid hormones 7-alpha-hydroxylase
Testosterone 7-alpha-hydroxylase

Gene names

Name:	Cyp2a1
Synonyms:	Cyp2a-1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Highly active in the 7-alpha-hydroxylation of testosterone, progesterone and androstenedione.
Catalytic activity	RH + reduced flavoprotein + O₂ = ROH + oxidized flavoprotein + H₂O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Liver and testis.
Induction	By 3-methylcholanthrene (3MC).
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	coumarin metabolic process Inferred from direct assay PubMed 12162851. Source: RGD
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell intracellular membrane-bounded organelle Inferred from direct assay Ref.1. Source: RGD
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro steroid hydroxylase activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 492

492

Cytochrome P450 2A1

PRO_0000051663

Sites

Metal binding

437

Iron (heme axial ligand)

Experimental info

Sequence conflict

386

G → A in AAA41030. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P11711 [UniParc].

Last modified May 1, 2007. Version 2.
Checksum: 58A187F9F10A1D85
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FASTA

492

55,995

        10         20         30         40         50         60 
MLDTGLLLVV ILASLSVMLL VSLWQQKIRG RLPPGPTPLP FIGNYLQLNT KDVYSSITQL 

        70         80         90        100        110        120 
SERYGPVFTI HLGPRRVVVL YGYDAVKEAL VDQAEEFSGR GEQATYNTLF KGYGVAFSSG 

       130        140        150        160        170        180 
ERAKQLRRLS IATLRDFGVG KRGVEERILE EAGYLIKMLQ GTCGAPIDPT IYLSKTVSNV 

       190        200        210        220        230        240 
ISSIVFGERF DYEDTEFLSL LQMMGQMNRF AASPTGQLYD MFHSVMKYLP GPQQQIIKVT 

       250        260        270        280        290        300 
QKLEDFMIEK VRQNHSTLDP NSPRNFIDSF LIRMQEEKNG NSEFHMKNLV MTTLSLFFAG 

       310        320        330        340        350        360 
SETVSSTLRY GFLLLMKHPD VEAKVHEEIE QVIGRNRQPQ YEDHMKMPYT QAVINEIQRF 

       370        380        390        400        410        420 
SNLAPLGIPR RIIKNTTFRG FFLPKGTDVF PILGSLMTDP KFFPSPKDFD PQNFLDDKGQ 

       430        440        450        460        470        480 
LKKNAAFLPF STGKRFCLGD GLAKMELFLL LTTILQNFRF KFPMKLEDIN ESPKPLGFTR 

       490 
IIPKYTMSFM PI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rat testosterone 7 alpha-hydroxylase. Isolation, sequence, and expression of cDNA and its developmental regulation and induction by 3-methylcholanthrene." Nagata K., Matsunaga T., Gillette J., Gelboin H.V., Gonzalez F.J. J. Biol. Chem. 262:2787-2793(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Structure and in vitro transcription of the rat CYP2A1 and CYP2A2 genes and regional localization of the CYP2A gene subfamily on mouse chromosome 7." Matsunaga T., Nomoto M., Kozak C.A., Gonzalez F.J. Biochemistry 29:1329-1341(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	"Purification of two isozymes of rat liver microsomal cytochrome P450 with testosterone 7 alpha-hydroxylase activity." Arlotto M.P., Greenway D.J., Parkinson A. Arch. Biochem. Biophys. 270:441-457(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-20. Tissue: Liver.
[5]	"Purification and characterization of six cytochromes P-450 from hepatic microsomes of immature female rats." Imaoka S., Kamataki T., Funae Y. J. Biochem. 102:843-851(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02669 mRNA. Translation: AAA41030.1. M33312 Genomic DNA. Translation: AAA41020.1. BC081848 mRNA. Translation: AAH81848.1.
IPI	IPI00196696.
PIR	A34272.
RefSeq	NP_036824.1. NM_012692.1.
UniGene	Rn.32107.
3D structure databases
ProteinModelPortal	P11711.
SMR	P11711. Positions 30-491.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4576797.
STRING	10116.ENSRNOP00000028237.
Proteomic databases
PaxDb	P11711.
PRIDE	P11711.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000028237; ENSRNOP00000028237; ENSRNOG00000020928.
GeneID	24894.
KEGG	rno:24894.
UCSC	RGD:2463. rat.
Organism-specific databases
CTD	24894.
RGD	2463. Cyp2a1.
Phylogenomic databases
eggNOG	COG2124.
GeneTree	ENSGT00670000097868.
HOGENOM	HOG000036992.
HOVERGEN	HBG015789.
InParanoid	P11711.
KO	K07411.
OMA	PTIYLSK.
OrthoDB	EOG4WM4WJ.
Gene expression databases
Genevestigator	P11711.
GermOnline	ENSRNOG00000020928. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008067. Cyt_P450_E_grp-I_CYP2A-like. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01684. EP450ICYP2A. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	604766.

Entry information

Entry name

CP2A1_RAT

Accession

Primary (citable) accession number: P11711
Secondary accession number(s): Q642C5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	May 1, 2007
Last modified:	May 29, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents