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P11711 (CP2A1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2A1

EC=1.14.14.1
Alternative name(s):
CYPIIA1
Cytochrome P450-UT-F
Steroid hormones 7-alpha-hydroxylase
Testosterone 7-alpha-hydroxylase
Gene names
Name:Cyp2a1
Synonyms:Cyp2a-1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Highly active in the 7-alpha-hydroxylation of testosterone, progesterone and androstenedione.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Liver and testis.

Induction

By 3-methylcholanthrene (3MC).

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 492492Cytochrome P450 2A1
PRO_0000051663

Sites

Metal binding4371Iron (heme axial ligand)

Experimental info

Sequence conflict3861G → A in AAA41030. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P11711 [UniParc].

Last modified May 1, 2007. Version 2.
Checksum: 58A187F9F10A1D85

FASTA49255,995
        10         20         30         40         50         60 
MLDTGLLLVV ILASLSVMLL VSLWQQKIRG RLPPGPTPLP FIGNYLQLNT KDVYSSITQL 

        70         80         90        100        110        120 
SERYGPVFTI HLGPRRVVVL YGYDAVKEAL VDQAEEFSGR GEQATYNTLF KGYGVAFSSG 

       130        140        150        160        170        180 
ERAKQLRRLS IATLRDFGVG KRGVEERILE EAGYLIKMLQ GTCGAPIDPT IYLSKTVSNV 

       190        200        210        220        230        240 
ISSIVFGERF DYEDTEFLSL LQMMGQMNRF AASPTGQLYD MFHSVMKYLP GPQQQIIKVT 

       250        260        270        280        290        300 
QKLEDFMIEK VRQNHSTLDP NSPRNFIDSF LIRMQEEKNG NSEFHMKNLV MTTLSLFFAG 

       310        320        330        340        350        360 
SETVSSTLRY GFLLLMKHPD VEAKVHEEIE QVIGRNRQPQ YEDHMKMPYT QAVINEIQRF 

       370        380        390        400        410        420 
SNLAPLGIPR RIIKNTTFRG FFLPKGTDVF PILGSLMTDP KFFPSPKDFD PQNFLDDKGQ 

       430        440        450        460        470        480 
LKKNAAFLPF STGKRFCLGD GLAKMELFLL LTTILQNFRF KFPMKLEDIN ESPKPLGFTR 

       490 
IIPKYTMSFM PI 

« Hide

References

« Hide 'large scale' references
[1]"Rat testosterone 7 alpha-hydroxylase. Isolation, sequence, and expression of cDNA and its developmental regulation and induction by 3-methylcholanthrene."
Nagata K., Matsunaga T., Gillette J., Gelboin H.V., Gonzalez F.J.
J. Biol. Chem. 262:2787-2793(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"Structure and in vitro transcription of the rat CYP2A1 and CYP2A2 genes and regional localization of the CYP2A gene subfamily on mouse chromosome 7."
Matsunaga T., Nomoto M., Kozak C.A., Gonzalez F.J.
Biochemistry 29:1329-1341(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[4]"Purification of two isozymes of rat liver microsomal cytochrome P450 with testosterone 7 alpha-hydroxylase activity."
Arlotto M.P., Greenway D.J., Parkinson A.
Arch. Biochem. Biophys. 270:441-457(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-20.
Tissue: Liver.
[5]"Purification and characterization of six cytochromes P-450 from hepatic microsomes of immature female rats."
Imaoka S., Kamataki T., Funae Y.
J. Biochem. 102:843-851(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-12.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02669 mRNA. Translation: AAA41030.1.
M33312 Genomic DNA. Translation: AAA41020.1.
BC081848 mRNA. Translation: AAH81848.1.
PIRA34272.
RefSeqNP_036824.1. NM_012692.1.
UniGeneRn.32107.

3D structure databases

ProteinModelPortalP11711.
SMRP11711. Positions 30-491.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4576797.
STRING10116.ENSRNOP00000028237.

Proteomic databases

PaxDbP11711.
PRIDEP11711.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000028237; ENSRNOP00000028237; ENSRNOG00000020928.
GeneID24894.
KEGGrno:24894.
UCSCRGD:2463. rat.

Organism-specific databases

CTD24894.
RGD2463. Cyp2a1.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00670000097868.
HOGENOMHOG000036992.
HOVERGENHBG015789.
InParanoidP11711.
KOK07411.
OMAPTIYLSK.
OrthoDBEOG7RBZ85.
PhylomeDBP11711.
TreeFamTF352043.

Gene expression databases

GenevestigatorP11711.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio604766.
PROP11711.

Entry information

Entry nameCP2A1_RAT
AccessionPrimary (citable) accession number: P11711
Secondary accession number(s): Q642C5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: May 1, 2007
Last modified: April 16, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families