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P11710 (FUS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear fusion protein FUS1
Gene names
Name:FUS1
Ordered Locus Names:YCL027W
ORF Names:YCL27W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for cell fusion. Negatively regulates Sho1p signaling to ensure efficient cell fusion. Ref.7

Interacts with SHO1. Ref.7

Subcellular location

Membrane; Single-pass membrane protein Potential.

Induction

FUS1 remains essentially unexpressed in vegetative cells, but is strongly induced by incubation of haploid cells with the appropriate mating pheromone.

Post-translational modification

O-glycosylated. Ref.6

Sequence similarities

Contains 1 SH3 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 512512Nuclear fusion protein FUS1
PRO_0000087384

Regions

Transmembrane72 – 9625Helical; Potential
Domain436 – 51277SH3
Region97 – 512416Hydrophilic
Compositional bias1 – 7171Ser/Thr-rich

Amino acid modifications

Modified residue1781Phosphothreonine Ref.8
Modified residue1901Phosphoserine Ref.8
Modified residue2561Phosphoserine Ref.8
Modified residue2811Phosphothreonine Ref.8
Modified residue4241Phosphothreonine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P11710 [UniParc].

Last modified September 26, 2001. Version 3.
Checksum: 705241556DAD5D1C

FASTA51257,779
        10         20         30         40         50         60 
MVATIMQTTT TVLTTVAAMS TTLASNYISS QASSSTSVTT VTTIATSIRS TPSNLLFSNV 

        70         80         90        100        110        120 
AAQPKSSSAS TIGLSIGLPI GIFCFGLLIL LCYFYLKRNS VSISNPPMSA TIPREEEYCR 

       130        140        150        160        170        180 
RTNWFSRLFW QSKCEDQNSY SNRDIEKYND TQWTSGDNMS SKIQYKISKP IIPQHILTPK 

       190        200        210        220        230        240 
KTVKNPYAWS GKNISLDPKV NEMEEEKVVD AFLYTKPPNI VHIESSMPSY NDLPSQKTVS 

       250        260        270        280        290        300 
SKKTALKTSE KWSYESPLSR WFLRGSTYFK DYGLSKTSLK TPTGAPQLKQ MKMLSRISKG 

       310        320        330        340        350        360 
YFNESDIMPD ERSPILEYNN TPLDANDSVN NLGNTTPDSQ ITSYRNNNID LITARPHSVI 

       370        380        390        400        410        420 
YGTTAQQTLE TNFNDHHDCN KSTEKHELII PTPSKPLKKR KKRRQSKMYQ HLQHLSRSKP 

       430        440        450        460        470        480 
LPLTPNSKYN GEASVQLGKT YTVIQDYEPR LTDEIRISLG EKVKILATHT DGWCLVEKCN 

       490        500        510 
TQKGSIHVSV DDKRYLNEDR GIVPGDCLQE YD 

« Hide

References

« Hide 'large scale' references
[1]"Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein."
Trueheart J., Boeke J.D., Fink G.R.
Mol. Cell. Biol. 7:2316-2328(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae."
McCaffrey G., Clay F.J., Kelsay K., Sprague G.F. Jr.
Mol. Cell. Biol. 7:2680-2690(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Mewes H.-W.
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 130; 482 AND 485.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"The yeast cell fusion protein FUS1 is O-glycosylated and spans the plasma membrane."
Trueheart J., Fink G.R.
Proc. Natl. Acad. Sci. U.S.A. 86:9916-9920(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION.
[7]"Fus1p interacts with components of the Hog1p mitogen-activated protein kinase and Cdc42p morphogenesis signaling pathways to control cell fusion during yeast mating."
Nelson B., Parsons A.B., Evangelista M., Schaefer K., Kennedy K., Ritchie S., Petryshen T.L., Boone C.
Genetics 166:67-77(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SHO1.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-178; SER-190; SER-256; THR-281 AND THR-424, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M17199 Genomic DNA. Translation: AAA34612.1.
M16717 Genomic DNA. Translation: AAA34616.1.
X59720 Genomic DNA. Translation: CAA42358.2.
BK006937 Genomic DNA. Translation: DAA07457.1.
PIRS19354.
RefSeqNP_009903.2. NM_001178672.1.

3D structure databases

ProteinModelPortalP11710.
SMRP11710. Positions 439-512.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid30956. 51 interactions.
DIPDIP-670N.
IntActP11710. 48 interactions.
MINTMINT-522625.
STRING4932.YCL027W.

Proteomic databases

PaxDbP11710.
PeptideAtlasP11710.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCL027W; YCL027W; YCL027W.
GeneID850330.
KEGGsce:YCL027W.

Organism-specific databases

CYGDYCL027w.
SGDS000000532. FUS1.

Phylogenomic databases

eggNOGNOG46565.
HOGENOMHOG000000893.
KOK11243.
OMAILARANK.
OrthoDBEOG7Z3FF0.

Enzyme and pathway databases

BioCycYEAST:G3O-29288-MONOMER.

Gene expression databases

GenevestigatorP11710.

Family and domain databases

InterProIPR001452. SH3_domain.
[Graphical view]
PfamPF00018. SH3_1. 1 hit.
[Graphical view]
SMARTSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
PROSITEPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio965760.

Entry information

Entry nameFUS1_YEAST
AccessionPrimary (citable) accession number: P11710
Secondary accession number(s): D6VQY8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: September 26, 2001
Last modified: June 11, 2014
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families