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P11710

- FUS1_YEAST

UniProt

P11710 - FUS1_YEAST

Protein

Nuclear fusion protein FUS1

Gene

FUS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 3 (26 Sep 2001)
      Previous versions | rss
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    Functioni

    Required for cell fusion. Negatively regulates Sho1p signaling to ensure efficient cell fusion.1 Publication
    Interacts with SHO1.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. cortical protein anchoring Source: SGD
    2. cytogamy Source: SGD
    3. regulation of termination of mating projection growth Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29288-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear fusion protein FUS1
    Gene namesi
    Name:FUS1
    Ordered Locus Names:YCL027W
    ORF Names:YCL27W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCL027w.
    SGDiS000000532. FUS1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: SGD
    2. mating projection tip Source: SGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 512512Nuclear fusion protein FUS1PRO_0000087384Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei178 – 1781Phosphothreonine1 Publication
    Modified residuei190 – 1901Phosphoserine1 Publication
    Modified residuei256 – 2561Phosphoserine1 Publication
    Modified residuei281 – 2811Phosphothreonine1 Publication
    Modified residuei424 – 4241Phosphothreonine1 Publication

    Post-translational modificationi

    O-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP11710.
    PeptideAtlasiP11710.

    Expressioni

    Inductioni

    FUS1 remains essentially unexpressed in vegetative cells, but is strongly induced by incubation of haploid cells with the appropriate mating pheromone.

    Gene expression databases

    GenevestigatoriP11710.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BNR1P404505EBI-7179,EBI-3711
    HAA1Q127533EBI-7179,EBI-8118
    PEA2P400915EBI-7179,EBI-13106
    SHO1P400737EBI-7179,EBI-18140
    STE5P329172EBI-7179,EBI-18373

    Protein-protein interaction databases

    BioGridi30956. 51 interactions.
    DIPiDIP-670N.
    IntActiP11710. 48 interactions.
    MINTiMINT-522625.
    STRINGi4932.YCL027W.

    Structurei

    3D structure databases

    ProteinModelPortaliP11710.
    SMRiP11710. Positions 439-512.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei72 – 9625HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini436 – 51277SH3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni97 – 512416HydrophilicAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1 – 7171Ser/Thr-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG46565.
    HOGENOMiHOG000000893.
    KOiK11243.
    OMAiILARANK.
    OrthoDBiEOG7Z3FF0.

    Family and domain databases

    InterProiIPR001452. SH3_domain.
    [Graphical view]
    PfamiPF00018. SH3_1. 1 hit.
    [Graphical view]
    SMARTiSM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P11710-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVATIMQTTT TVLTTVAAMS TTLASNYISS QASSSTSVTT VTTIATSIRS    50
    TPSNLLFSNV AAQPKSSSAS TIGLSIGLPI GIFCFGLLIL LCYFYLKRNS 100
    VSISNPPMSA TIPREEEYCR RTNWFSRLFW QSKCEDQNSY SNRDIEKYND 150
    TQWTSGDNMS SKIQYKISKP IIPQHILTPK KTVKNPYAWS GKNISLDPKV 200
    NEMEEEKVVD AFLYTKPPNI VHIESSMPSY NDLPSQKTVS SKKTALKTSE 250
    KWSYESPLSR WFLRGSTYFK DYGLSKTSLK TPTGAPQLKQ MKMLSRISKG 300
    YFNESDIMPD ERSPILEYNN TPLDANDSVN NLGNTTPDSQ ITSYRNNNID 350
    LITARPHSVI YGTTAQQTLE TNFNDHHDCN KSTEKHELII PTPSKPLKKR 400
    KKRRQSKMYQ HLQHLSRSKP LPLTPNSKYN GEASVQLGKT YTVIQDYEPR 450
    LTDEIRISLG EKVKILATHT DGWCLVEKCN TQKGSIHVSV DDKRYLNEDR 500
    GIVPGDCLQE YD 512
    Length:512
    Mass (Da):57,779
    Last modified:September 26, 2001 - v3
    Checksum:i705241556DAD5D1C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17199 Genomic DNA. Translation: AAA34612.1.
    M16717 Genomic DNA. Translation: AAA34616.1.
    X59720 Genomic DNA. Translation: CAA42358.2.
    BK006937 Genomic DNA. Translation: DAA07457.1.
    PIRiS19354.
    RefSeqiNP_009903.2. NM_001178672.1.

    Genome annotation databases

    EnsemblFungiiYCL027W; YCL027W; YCL027W.
    GeneIDi850330.
    KEGGisce:YCL027W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17199 Genomic DNA. Translation: AAA34612.1 .
    M16717 Genomic DNA. Translation: AAA34616.1 .
    X59720 Genomic DNA. Translation: CAA42358.2 .
    BK006937 Genomic DNA. Translation: DAA07457.1 .
    PIRi S19354.
    RefSeqi NP_009903.2. NM_001178672.1.

    3D structure databases

    ProteinModelPortali P11710.
    SMRi P11710. Positions 439-512.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 30956. 51 interactions.
    DIPi DIP-670N.
    IntActi P11710. 48 interactions.
    MINTi MINT-522625.
    STRINGi 4932.YCL027W.

    Proteomic databases

    PaxDbi P11710.
    PeptideAtlasi P11710.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCL027W ; YCL027W ; YCL027W .
    GeneIDi 850330.
    KEGGi sce:YCL027W.

    Organism-specific databases

    CYGDi YCL027w.
    SGDi S000000532. FUS1.

    Phylogenomic databases

    eggNOGi NOG46565.
    HOGENOMi HOG000000893.
    KOi K11243.
    OMAi ILARANK.
    OrthoDBi EOG7Z3FF0.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29288-MONOMER.

    Miscellaneous databases

    NextBioi 965760.

    Gene expression databases

    Genevestigatori P11710.

    Family and domain databases

    InterProi IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF00018. SH3_1. 1 hit.
    [Graphical view ]
    SMARTi SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein."
      Trueheart J., Boeke J.D., Fink G.R.
      Mol. Cell. Biol. 7:2316-2328(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae."
      McCaffrey G., Clay F.J., Kelsay K., Sprague G.F. Jr.
      Mol. Cell. Biol. 7:2680-2690(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Mewes H.-W.
      Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 130; 482 AND 485.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "The yeast cell fusion protein FUS1 is O-glycosylated and spans the plasma membrane."
      Trueheart J., Fink G.R.
      Proc. Natl. Acad. Sci. U.S.A. 86:9916-9920(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION.
    7. "Fus1p interacts with components of the Hog1p mitogen-activated protein kinase and Cdc42p morphogenesis signaling pathways to control cell fusion during yeast mating."
      Nelson B., Parsons A.B., Evangelista M., Schaefer K., Kennedy K., Ritchie S., Petryshen T.L., Boone C.
      Genetics 166:67-77(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SHO1.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-178; SER-190; SER-256; THR-281 AND THR-424, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.

    Entry informationi

    Entry nameiFUS1_YEAST
    AccessioniPrimary (citable) accession number: P11710
    Secondary accession number(s): D6VQY8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: September 26, 2001
    Last modified: October 1, 2014
    This is version 121 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3