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P11710

- FUS1_YEAST

UniProt

P11710 - FUS1_YEAST

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Protein
Nuclear fusion protein FUS1
Gene
FUS1, YCL027W, YCL27W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for cell fusion. Negatively regulates Sho1p signaling to ensure efficient cell fusion.1 Publication
Interacts with SHO1.1 Publication

GO - Molecular functioni

  1. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. cortical protein anchoring Source: SGD
  2. cytogamy Source: SGD
  3. regulation of termination of mating projection growth Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29288-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear fusion protein FUS1
Gene namesi
Name:FUS1
Ordered Locus Names:YCL027W
ORF Names:YCL27W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCL027w.
SGDiS000000532. FUS1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei72 – 9625Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: SGD
  2. mating projection tip Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 512512Nuclear fusion protein FUS1
PRO_0000087384Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei178 – 1781Phosphothreonine1 Publication
Modified residuei190 – 1901Phosphoserine1 Publication
Modified residuei256 – 2561Phosphoserine1 Publication
Modified residuei281 – 2811Phosphothreonine1 Publication
Modified residuei424 – 4241Phosphothreonine1 Publication

Post-translational modificationi

O-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP11710.
PeptideAtlasiP11710.

Expressioni

Inductioni

FUS1 remains essentially unexpressed in vegetative cells, but is strongly induced by incubation of haploid cells with the appropriate mating pheromone.

Gene expression databases

GenevestigatoriP11710.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
BNR1P404505EBI-7179,EBI-3711
HAA1Q127533EBI-7179,EBI-8118
PEA2P400915EBI-7179,EBI-13106
SHO1P400737EBI-7179,EBI-18140
STE5P329172EBI-7179,EBI-18373

Protein-protein interaction databases

BioGridi30956. 51 interactions.
DIPiDIP-670N.
IntActiP11710. 48 interactions.
MINTiMINT-522625.
STRINGi4932.YCL027W.

Structurei

3D structure databases

ProteinModelPortaliP11710.
SMRiP11710. Positions 439-512.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini436 – 51277SH3
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni97 – 512416Hydrophilic
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 7171Ser/Thr-rich
Add
BLAST

Sequence similaritiesi

Contains 1 SH3 domain.

Keywords - Domaini

SH3 domain, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG46565.
HOGENOMiHOG000000893.
KOiK11243.
OMAiILARANK.
OrthoDBiEOG7Z3FF0.

Family and domain databases

InterProiIPR001452. SH3_domain.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11710-1 [UniParc]FASTAAdd to Basket

« Hide

MVATIMQTTT TVLTTVAAMS TTLASNYISS QASSSTSVTT VTTIATSIRS    50
TPSNLLFSNV AAQPKSSSAS TIGLSIGLPI GIFCFGLLIL LCYFYLKRNS 100
VSISNPPMSA TIPREEEYCR RTNWFSRLFW QSKCEDQNSY SNRDIEKYND 150
TQWTSGDNMS SKIQYKISKP IIPQHILTPK KTVKNPYAWS GKNISLDPKV 200
NEMEEEKVVD AFLYTKPPNI VHIESSMPSY NDLPSQKTVS SKKTALKTSE 250
KWSYESPLSR WFLRGSTYFK DYGLSKTSLK TPTGAPQLKQ MKMLSRISKG 300
YFNESDIMPD ERSPILEYNN TPLDANDSVN NLGNTTPDSQ ITSYRNNNID 350
LITARPHSVI YGTTAQQTLE TNFNDHHDCN KSTEKHELII PTPSKPLKKR 400
KKRRQSKMYQ HLQHLSRSKP LPLTPNSKYN GEASVQLGKT YTVIQDYEPR 450
LTDEIRISLG EKVKILATHT DGWCLVEKCN TQKGSIHVSV DDKRYLNEDR 500
GIVPGDCLQE YD 512
Length:512
Mass (Da):57,779
Last modified:September 26, 2001 - v3
Checksum:i705241556DAD5D1C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17199 Genomic DNA. Translation: AAA34612.1.
M16717 Genomic DNA. Translation: AAA34616.1.
X59720 Genomic DNA. Translation: CAA42358.2.
BK006937 Genomic DNA. Translation: DAA07457.1.
PIRiS19354.
RefSeqiNP_009903.2. NM_001178672.1.

Genome annotation databases

EnsemblFungiiYCL027W; YCL027W; YCL027W.
GeneIDi850330.
KEGGisce:YCL027W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17199 Genomic DNA. Translation: AAA34612.1 .
M16717 Genomic DNA. Translation: AAA34616.1 .
X59720 Genomic DNA. Translation: CAA42358.2 .
BK006937 Genomic DNA. Translation: DAA07457.1 .
PIRi S19354.
RefSeqi NP_009903.2. NM_001178672.1.

3D structure databases

ProteinModelPortali P11710.
SMRi P11710. Positions 439-512.
ModBasei Search...

Protein-protein interaction databases

BioGridi 30956. 51 interactions.
DIPi DIP-670N.
IntActi P11710. 48 interactions.
MINTi MINT-522625.
STRINGi 4932.YCL027W.

Proteomic databases

PaxDbi P11710.
PeptideAtlasi P11710.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCL027W ; YCL027W ; YCL027W .
GeneIDi 850330.
KEGGi sce:YCL027W.

Organism-specific databases

CYGDi YCL027w.
SGDi S000000532. FUS1.

Phylogenomic databases

eggNOGi NOG46565.
HOGENOMi HOG000000893.
KOi K11243.
OMAi ILARANK.
OrthoDBi EOG7Z3FF0.

Enzyme and pathway databases

BioCyci YEAST:G3O-29288-MONOMER.

Miscellaneous databases

NextBioi 965760.

Gene expression databases

Genevestigatori P11710.

Family and domain databases

InterProi IPR001452. SH3_domain.
[Graphical view ]
Pfami PF00018. SH3_1. 1 hit.
[Graphical view ]
SMARTi SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
PROSITEi PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein."
    Trueheart J., Boeke J.D., Fink G.R.
    Mol. Cell. Biol. 7:2316-2328(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae."
    McCaffrey G., Clay F.J., Kelsay K., Sprague G.F. Jr.
    Mol. Cell. Biol. 7:2680-2690(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Mewes H.-W.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 130; 482 AND 485.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "The yeast cell fusion protein FUS1 is O-glycosylated and spans the plasma membrane."
    Trueheart J., Fink G.R.
    Proc. Natl. Acad. Sci. U.S.A. 86:9916-9920(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION.
  7. "Fus1p interacts with components of the Hog1p mitogen-activated protein kinase and Cdc42p morphogenesis signaling pathways to control cell fusion during yeast mating."
    Nelson B., Parsons A.B., Evangelista M., Schaefer K., Kennedy K., Ritchie S., Petryshen T.L., Boone C.
    Genetics 166:67-77(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SHO1.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-178; SER-190; SER-256; THR-281 AND THR-424, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.

Entry informationi

Entry nameiFUS1_YEAST
AccessioniPrimary (citable) accession number: P11710
Secondary accession number(s): D6VQY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: September 26, 2001
Last modified: June 11, 2014
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

Similar proteinsi