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P11709 (BIK1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear fusion protein BIK1
Gene names
Name:BIK1
Ordered Locus Names:YCL029C
ORF Names:YCL29C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length440 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for nuclear fusion, chromosome disjunction, and nuclear segregation during mitosis. Probably required for the formation or stabilization of microtubules during mitosis and for spindle pole body fusion during conjugation. Ref.5

Subcellular location

Cytoplasmcytoskeletonmicrotubule organizing centerspindle pole body. Cytoplasmcytoskeletonspindle. Cytoplasm. Note: And mitotic spindle.

Miscellaneous

Present with 300 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 CAP-Gly domain.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
   Cellular componentCytoplasm
Cytoskeleton
Microtubule
   DomainCoiled coil
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processkaryogamy involved in conjugation with cellular fusion

Inferred from mutant phenotype Ref.5. Source: SGD

mitosis

Inferred from electronic annotation. Source: UniProtKB-KW

mitotic spindle elongation

Inferred from genetic interaction PubMed 7559759. Source: SGD

negative regulation of microtubule polymerization

Inferred from direct assay PubMed 20392838. Source: SGD

nuclear migration involved in conjugation with cellular fusion

Inferred from mutant phenotype PubMed 16380440. Source: SGD

   Cellular_componentcell cortex

Inferred from direct assay PubMed 11511347. Source: SGD

condensed nuclear chromosome kinetochore

Inferred from direct assay PubMed 11511347. Source: SGD

kinetochore

Inferred from direct assay PubMed 11756471. Source: SGD

mating projection tip

Inferred from direct assay PubMed 16380440. Source: SGD

microtubule plus-end

Inferred from direct assay PubMed 20392838. Source: SGD

spindle

Inferred from direct assay Ref.5. Source: SGD

spindle microtubule

Inferred from direct assay PubMed 11511347. Source: SGD

spindle pole body

Inferred from direct assay Ref.5. Source: SGD

   Molecular_functionmicrotubule binding

Inferred from genetic interaction Ref.5. Source: SGD

protein homodimerization activity

Inferred from direct assay PubMed 20392838. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 440440Nuclear fusion protein BIK1
PRO_0000083513

Regions

Domain26 – 6944CAP-Gly
Coiled coil190 – 397208 Potential
Motif416 – 42914CCHC-box

Amino acid modifications

Modified residue951Phosphoserine Ref.8
Modified residue1101Phosphoserine Ref.7 Ref.8

Sequences

Sequence LengthMass (Da)Tools
P11709 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: 9E098B25C431619B

FASTA44051,092
        10         20         30         40         50         60 
MDRYQRKIGC FIQIPNLGRG QLKYVGPVDT KAGMFAGVDL LANIGKNDGS FMGKKYFQTE 

        70         80         90        100        110        120 
YPQSGLFIQL QKVASLIEKA SISQTSRRTT MEPLSIPKNR SIVRLTNQFS PMDDPKSPTP 

       130        140        150        160        170        180 
MRSFRITSRH SGNQQSMDQE ASDHHQQQEF GYDNREDRME VDSILSSDRK ANHNTTSDWK 

       190        200        210        220        230        240 
PDNGHMNDLN SSEVTIELRE AQLTIEKLQR KQLHYKRLLD DQRMVLEEVQ PTFDRYEATI 

       250        260        270        280        290        300 
QEREKEIDHL KQQLELERRQ QAKQKQFFDA ENEQLLAVVS QLHEEIKENE ERNLSHNQPT 

       310        320        330        340        350        360 
GANEDVELLK KQLEQLRNIE DQFELHKTKW AKEREQLKMH NDSLSKEYQN LSKELFLTKP 

       370        380        390        400        410        420 
QDSSSEEVAS LTKKLEEANE KIKQLEQAQA QTAVESLPIF DPPAPVDTTA GRQQWCEHCD 

       430        440 
TMGHNTAECP HHNPDNQQFF 

« Hide

References

« Hide 'large scale' references
[1]"Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein."
Trueheart J., Boeke J.D., Fink G.R.
Mol. Cell. Biol. 7:2316-2328(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete sequence of a 11,953 bp fragment from C1G on chromosome III encompasses four new open reading frames."
Rad M.R., Luetzenkirchen K., Xu G., Kleinhans U., Hollenberg C.P.
Yeast 7:533-538(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"BIK1, a protein required for microtubule function during mating and mitosis in Saccharomyces cerevisiae, colocalizes with tubulin."
Berlin V., Styles C.A., Fink G.R.
J. Cell Biol. 111:2573-2586(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: POSSIBLE FUNCTION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95 AND SER-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M16717 Genomic DNA. Translation: AAA34614.1.
X59720 Genomic DNA. Translation: CAA42356.1.
BK006937 Genomic DNA. Translation: DAA07455.1.
PIRB27061.
RefSeqNP_009901.1. NM_001178674.1.

3D structure databases

ProteinModelPortalP11709.
SMRP11709. Positions 20-73.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid30954. 151 interactions.
DIPDIP-2341N.
IntActP11709. 4 interactions.
MINTMINT-437612.
STRING4932.YCL029C.

Proteomic databases

PaxDbP11709.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCL029C; YCL029C; YCL029C.
GeneID850328.
KEGGsce:YCL029C.

Organism-specific databases

CYGDYCL029c.
SGDS000000534. BIK1.

Phylogenomic databases

eggNOGNOG254865.
HOGENOMHOG000057099.
OMAPYENDIF.
OrthoDBEOG7HF1VC.

Enzyme and pathway databases

BioCycYEAST:G3O-29290-MONOMER.

Gene expression databases

GenevestigatorP11709.

Family and domain databases

Gene3D2.30.30.190. 1 hit.
InterProIPR000938. CAP-Gly_domain.
[Graphical view]
PfamPF01302. CAP_GLY. 1 hit.
[Graphical view]
SMARTSM01052. CAP_GLY. 1 hit.
[Graphical view]
SUPFAMSSF74924. SSF74924. 1 hit.
PROSITEPS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio965754.

Entry information

Entry nameBIK1_YEAST
AccessionPrimary (citable) accession number: P11709
Secondary accession number(s): D6VQY6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: April 16, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families