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P11709

- BIK1_YEAST

UniProt

P11709 - BIK1_YEAST

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Protein

Nuclear fusion protein BIK1

Gene

BIK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for nuclear fusion, chromosome disjunction, and nuclear segregation during mitosis. Probably required for the formation or stabilization of microtubules during mitosis and for spindle pole body fusion during conjugation.

GO - Molecular functioni

  1. microtubule binding Source: SGD
  2. protein homodimerization activity Source: SGD

GO - Biological processi

  1. karyogamy involved in conjugation with cellular fusion Source: SGD
  2. mitotic nuclear division Source: UniProtKB-KW
  3. mitotic spindle elongation Source: SGD
  4. negative regulation of microtubule polymerization Source: SGD
  5. nuclear migration involved in conjugation with cellular fusion Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-29290-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear fusion protein BIK1
Gene namesi
Name:BIK1
Ordered Locus Names:YCL029C
ORF Names:YCL29C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCL029c.
SGDiS000000534. BIK1.

Subcellular locationi

GO - Cellular componenti

  1. cell cortex Source: SGD
  2. condensed nuclear chromosome kinetochore Source: SGD
  3. kinetochore Source: SGD
  4. mating projection tip Source: SGD
  5. microtubule plus-end Source: SGD
  6. spindle Source: SGD
  7. spindle microtubule Source: SGD
  8. spindle pole body Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440Nuclear fusion protein BIK1PRO_0000083513Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei95 – 951Phosphoserine1 Publication
Modified residuei110 – 1101Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP11709.
PaxDbiP11709.

Expressioni

Gene expression databases

GenevestigatoriP11709.

Interactioni

Protein-protein interaction databases

BioGridi30954. 152 interactions.
DIPiDIP-2341N.
IntActiP11709. 4 interactions.
MINTiMINT-437612.
STRINGi4932.YCL029C.

Structurei

3D structure databases

ProteinModelPortaliP11709.
SMRiP11709. Positions 20-73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 6944CAP-GlyPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili190 – 397208Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi416 – 42914CCHC-boxAdd
BLAST

Sequence similaritiesi

Contains 1 CAP-Gly domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG254865.
HOGENOMiHOG000057099.
InParanoidiP11709.
OMAiPYENDIF.
OrthoDBiEOG7HF1VC.

Family and domain databases

Gene3Di2.30.30.190. 1 hit.
InterProiIPR000938. CAP-Gly_domain.
[Graphical view]
PfamiPF01302. CAP_GLY. 1 hit.
[Graphical view]
SMARTiSM01052. CAP_GLY. 1 hit.
[Graphical view]
SUPFAMiSSF74924. SSF74924. 1 hit.
PROSITEiPS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11709-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDRYQRKIGC FIQIPNLGRG QLKYVGPVDT KAGMFAGVDL LANIGKNDGS
60 70 80 90 100
FMGKKYFQTE YPQSGLFIQL QKVASLIEKA SISQTSRRTT MEPLSIPKNR
110 120 130 140 150
SIVRLTNQFS PMDDPKSPTP MRSFRITSRH SGNQQSMDQE ASDHHQQQEF
160 170 180 190 200
GYDNREDRME VDSILSSDRK ANHNTTSDWK PDNGHMNDLN SSEVTIELRE
210 220 230 240 250
AQLTIEKLQR KQLHYKRLLD DQRMVLEEVQ PTFDRYEATI QEREKEIDHL
260 270 280 290 300
KQQLELERRQ QAKQKQFFDA ENEQLLAVVS QLHEEIKENE ERNLSHNQPT
310 320 330 340 350
GANEDVELLK KQLEQLRNIE DQFELHKTKW AKEREQLKMH NDSLSKEYQN
360 370 380 390 400
LSKELFLTKP QDSSSEEVAS LTKKLEEANE KIKQLEQAQA QTAVESLPIF
410 420 430 440
DPPAPVDTTA GRQQWCEHCD TMGHNTAECP HHNPDNQQFF
Length:440
Mass (Da):51,092
Last modified:October 1, 1989 - v1
Checksum:i9E098B25C431619B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16717 Genomic DNA. Translation: AAA34614.1.
X59720 Genomic DNA. Translation: CAA42356.1.
BK006937 Genomic DNA. Translation: DAA07455.1.
PIRiB27061.
RefSeqiNP_009901.1. NM_001178674.1.

Genome annotation databases

EnsemblFungiiYCL029C; YCL029C; YCL029C.
GeneIDi850328.
KEGGisce:YCL029C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16717 Genomic DNA. Translation: AAA34614.1 .
X59720 Genomic DNA. Translation: CAA42356.1 .
BK006937 Genomic DNA. Translation: DAA07455.1 .
PIRi B27061.
RefSeqi NP_009901.1. NM_001178674.1.

3D structure databases

ProteinModelPortali P11709.
SMRi P11709. Positions 20-73.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 30954. 152 interactions.
DIPi DIP-2341N.
IntActi P11709. 4 interactions.
MINTi MINT-437612.
STRINGi 4932.YCL029C.

Proteomic databases

MaxQBi P11709.
PaxDbi P11709.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCL029C ; YCL029C ; YCL029C .
GeneIDi 850328.
KEGGi sce:YCL029C.

Organism-specific databases

CYGDi YCL029c.
SGDi S000000534. BIK1.

Phylogenomic databases

eggNOGi NOG254865.
HOGENOMi HOG000057099.
InParanoidi P11709.
OMAi PYENDIF.
OrthoDBi EOG7HF1VC.

Enzyme and pathway databases

BioCyci YEAST:G3O-29290-MONOMER.

Miscellaneous databases

NextBioi 965754.

Gene expression databases

Genevestigatori P11709.

Family and domain databases

Gene3Di 2.30.30.190. 1 hit.
InterProi IPR000938. CAP-Gly_domain.
[Graphical view ]
Pfami PF01302. CAP_GLY. 1 hit.
[Graphical view ]
SMARTi SM01052. CAP_GLY. 1 hit.
[Graphical view ]
SUPFAMi SSF74924. SSF74924. 1 hit.
PROSITEi PS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein."
    Trueheart J., Boeke J.D., Fink G.R.
    Mol. Cell. Biol. 7:2316-2328(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete sequence of a 11,953 bp fragment from C1G on chromosome III encompasses four new open reading frames."
    Rad M.R., Luetzenkirchen K., Xu G., Kleinhans U., Hollenberg C.P.
    Yeast 7:533-538(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "BIK1, a protein required for microtubule function during mating and mitosis in Saccharomyces cerevisiae, colocalizes with tubulin."
    Berlin V., Styles C.A., Fink G.R.
    J. Cell Biol. 111:2573-2586(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE FUNCTION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95 AND SER-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBIK1_YEAST
AccessioniPrimary (citable) accession number: P11709
Secondary accession number(s): D6VQY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: October 29, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3