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P11709

- BIK1_YEAST

UniProt

P11709 - BIK1_YEAST

Protein

Nuclear fusion protein BIK1

Gene

BIK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Oct 1989)
      Previous versions | rss
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    Functioni

    Required for nuclear fusion, chromosome disjunction, and nuclear segregation during mitosis. Probably required for the formation or stabilization of microtubules during mitosis and for spindle pole body fusion during conjugation.

    GO - Molecular functioni

    1. microtubule binding Source: SGD
    2. protein homodimerization activity Source: SGD

    GO - Biological processi

    1. karyogamy involved in conjugation with cellular fusion Source: SGD
    2. mitotic nuclear division Source: UniProtKB-KW
    3. mitotic spindle elongation Source: SGD
    4. negative regulation of microtubule polymerization Source: SGD
    5. nuclear migration involved in conjugation with cellular fusion Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29290-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear fusion protein BIK1
    Gene namesi
    Name:BIK1
    Ordered Locus Names:YCL029C
    ORF Names:YCL29C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCL029c.
    SGDiS000000534. BIK1.

    Subcellular locationi

    GO - Cellular componenti

    1. cell cortex Source: SGD
    2. condensed nuclear chromosome kinetochore Source: SGD
    3. kinetochore Source: SGD
    4. mating projection tip Source: SGD
    5. microtubule plus-end Source: SGD
    6. spindle Source: SGD
    7. spindle microtubule Source: SGD
    8. spindle pole body Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 440440Nuclear fusion protein BIK1PRO_0000083513Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei95 – 951Phosphoserine1 Publication
    Modified residuei110 – 1101Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP11709.
    PaxDbiP11709.

    Expressioni

    Gene expression databases

    GenevestigatoriP11709.

    Interactioni

    Protein-protein interaction databases

    BioGridi30954. 152 interactions.
    DIPiDIP-2341N.
    IntActiP11709. 4 interactions.
    MINTiMINT-437612.
    STRINGi4932.YCL029C.

    Structurei

    3D structure databases

    ProteinModelPortaliP11709.
    SMRiP11709. Positions 20-73.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 6944CAP-GlyPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili190 – 397208Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi416 – 42914CCHC-boxAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CAP-Gly domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG254865.
    HOGENOMiHOG000057099.
    OMAiPYENDIF.
    OrthoDBiEOG7HF1VC.

    Family and domain databases

    Gene3Di2.30.30.190. 1 hit.
    InterProiIPR000938. CAP-Gly_domain.
    [Graphical view]
    PfamiPF01302. CAP_GLY. 1 hit.
    [Graphical view]
    SMARTiSM01052. CAP_GLY. 1 hit.
    [Graphical view]
    SUPFAMiSSF74924. SSF74924. 1 hit.
    PROSITEiPS00845. CAP_GLY_1. 1 hit.
    PS50245. CAP_GLY_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P11709-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDRYQRKIGC FIQIPNLGRG QLKYVGPVDT KAGMFAGVDL LANIGKNDGS    50
    FMGKKYFQTE YPQSGLFIQL QKVASLIEKA SISQTSRRTT MEPLSIPKNR 100
    SIVRLTNQFS PMDDPKSPTP MRSFRITSRH SGNQQSMDQE ASDHHQQQEF 150
    GYDNREDRME VDSILSSDRK ANHNTTSDWK PDNGHMNDLN SSEVTIELRE 200
    AQLTIEKLQR KQLHYKRLLD DQRMVLEEVQ PTFDRYEATI QEREKEIDHL 250
    KQQLELERRQ QAKQKQFFDA ENEQLLAVVS QLHEEIKENE ERNLSHNQPT 300
    GANEDVELLK KQLEQLRNIE DQFELHKTKW AKEREQLKMH NDSLSKEYQN 350
    LSKELFLTKP QDSSSEEVAS LTKKLEEANE KIKQLEQAQA QTAVESLPIF 400
    DPPAPVDTTA GRQQWCEHCD TMGHNTAECP HHNPDNQQFF 440
    Length:440
    Mass (Da):51,092
    Last modified:October 1, 1989 - v1
    Checksum:i9E098B25C431619B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16717 Genomic DNA. Translation: AAA34614.1.
    X59720 Genomic DNA. Translation: CAA42356.1.
    BK006937 Genomic DNA. Translation: DAA07455.1.
    PIRiB27061.
    RefSeqiNP_009901.1. NM_001178674.1.

    Genome annotation databases

    EnsemblFungiiYCL029C; YCL029C; YCL029C.
    GeneIDi850328.
    KEGGisce:YCL029C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16717 Genomic DNA. Translation: AAA34614.1 .
    X59720 Genomic DNA. Translation: CAA42356.1 .
    BK006937 Genomic DNA. Translation: DAA07455.1 .
    PIRi B27061.
    RefSeqi NP_009901.1. NM_001178674.1.

    3D structure databases

    ProteinModelPortali P11709.
    SMRi P11709. Positions 20-73.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 30954. 152 interactions.
    DIPi DIP-2341N.
    IntActi P11709. 4 interactions.
    MINTi MINT-437612.
    STRINGi 4932.YCL029C.

    Proteomic databases

    MaxQBi P11709.
    PaxDbi P11709.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCL029C ; YCL029C ; YCL029C .
    GeneIDi 850328.
    KEGGi sce:YCL029C.

    Organism-specific databases

    CYGDi YCL029c.
    SGDi S000000534. BIK1.

    Phylogenomic databases

    eggNOGi NOG254865.
    HOGENOMi HOG000057099.
    OMAi PYENDIF.
    OrthoDBi EOG7HF1VC.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29290-MONOMER.

    Miscellaneous databases

    NextBioi 965754.

    Gene expression databases

    Genevestigatori P11709.

    Family and domain databases

    Gene3Di 2.30.30.190. 1 hit.
    InterProi IPR000938. CAP-Gly_domain.
    [Graphical view ]
    Pfami PF01302. CAP_GLY. 1 hit.
    [Graphical view ]
    SMARTi SM01052. CAP_GLY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF74924. SSF74924. 1 hit.
    PROSITEi PS00845. CAP_GLY_1. 1 hit.
    PS50245. CAP_GLY_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein."
      Trueheart J., Boeke J.D., Fink G.R.
      Mol. Cell. Biol. 7:2316-2328(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete sequence of a 11,953 bp fragment from C1G on chromosome III encompasses four new open reading frames."
      Rad M.R., Luetzenkirchen K., Xu G., Kleinhans U., Hollenberg C.P.
      Yeast 7:533-538(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "BIK1, a protein required for microtubule function during mating and mitosis in Saccharomyces cerevisiae, colocalizes with tubulin."
      Berlin V., Styles C.A., Fink G.R.
      J. Cell Biol. 111:2573-2586(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: POSSIBLE FUNCTION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95 AND SER-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBIK1_YEAST
    AccessioniPrimary (citable) accession number: P11709
    Secondary accession number(s): D6VQY6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3