ID   P19_TBSVC               Reviewed;         172 AA.
AC   P11690;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   27-MAR-2024, entry version 83.
DE   RecName: Full=RNA silencing suppressor p19;
DE   AltName: Full=19 kDa symptom severity modulator;
GN   ORFNames=ORF4;
OS   Tomato bushy stunt virus (strain Cherry) (TBSV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes;
OC   Tolivirales; Tombusviridae; Procedovirinae; Tombusvirus;
OC   Tombusvirus lycopersici.
OX   NCBI_TaxID=12147;
OH   NCBI_TaxID=4072; Capsicum annuum (Capsicum pepper).
OH   NCBI_TaxID=3749; Malus.
OH   NCBI_TaxID=3766; Pyrus (pears).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH   NCBI_TaxID=4111; Solanum melongena (Eggplant) (Aubergine).
OH   NCBI_TaxID=29777; Tolmiea menziesii.
OH   NCBI_TaxID=13305; Tulipa.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2922927; DOI=10.1016/0042-6822(89)90039-1;
RA   Hillman B.I., Hearne P.Q., Rochon D., Morris T.J.;
RT   "Organization of tomato bushy stunt virus genome: characterization of the
RT   coat protein gene and the 3' terminus.";
RL   Virology 169:42-50(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2353450; DOI=10.1016/0042-6822(90)90468-7;
RA   Hearne P.Q., Knorr D.A., Hillman B.I., Morris T.J.;
RT   "The complete genome structure and synthesis of infectious RNA from clones
RT   of tomato bushy stunt virus.";
RL   Virology 177:141-151(1990).
RN   [3]
RP   FUNCTION.
RX   PubMed=10570213; DOI=10.1073/pnas.96.24.14147;
RA   Voinnet O., Pinto Y.M., Baulcombe D.C.;
RT   "Suppression of gene silencing: a general strategy used by diverse DNA and
RT   RNA viruses of plants.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:14147-14152(1999).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 23-158.
RX   PubMed=14661029; DOI=10.1038/nature02213;
RA   Ye K., Malinina L., Patel D.J.;
RT   "Recognition of small interfering RNA by a viral suppressor of RNA
RT   silencing.";
RL   Nature 426:874-878(2003).
CC   -!- FUNCTION: Acts as a suppressor of RNA-mediated gene silencing, also
CC       known as post-transcriptional gene silencing (PTGS), a mechanism of
CC       plant viral defense that limits the accumulation of viral RNAs. Binds
CC       to short interfering RNAs (siRNAs) with high affinity. Acts as a
CC       molecular caliper to specifically select siRNAs based on the length of
CC       the duplex region of the RNA (By similarity). {ECO:0000250,
CC       ECO:0000269|PubMed:10570213}.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the tombusviruses protein p19 family.
CC       {ECO:0000305}.
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DR   EMBL; M21958; AAB02538.1; -; Genomic_RNA.
DR   PIR; JA0116; NKVGTD.
DR   RefSeq; NP_062901.1; NC_001554.1.
DR   PDB; 1R9F; X-ray; 1.85 A; A=27-158.
DR   PDBsum; 1R9F; -.
DR   SMR; P11690; -.
DR   GeneID; 1493957; -.
DR   KEGG; vg:1493957; -.
DR   EvolutionaryTrace; P11690; -.
DR   Proteomes; UP000001666; Segment.
DR   GO; GO:0044423; C:virion component; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.390.180; RNA silencing suppressor P19; 1.
DR   InterPro; IPR004905; Tombusvirus_p19.
DR   InterPro; IPR036131; VP19_sf.
DR   Pfam; PF03220; Tombus_P19; 1.
DR   SUPFAM; SSF103145; Tombusvirus P19 core protein, VP19; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Host-virus interaction;
KW   Inhibition of host innate immune response by virus; Reference proteome;
KW   RNA-binding; Suppressor of RNA silencing; Viral immunoevasion.
FT   CHAIN           1..172
FT                   /note="RNA silencing suppressor p19"
FT                   /id="PRO_0000222881"
FT   REGION          1..38
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        19..34
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   HELIX           39..48
FT                   /evidence="ECO:0007829|PDB:1R9F"
FT   STRAND          57..65
FT                   /evidence="ECO:0007829|PDB:1R9F"
FT   STRAND          68..75
FT                   /evidence="ECO:0007829|PDB:1R9F"
FT   HELIX           80..87
FT                   /evidence="ECO:0007829|PDB:1R9F"
FT   HELIX           92..100
FT                   /evidence="ECO:0007829|PDB:1R9F"
FT   STRAND          109..116
FT                   /evidence="ECO:0007829|PDB:1R9F"
FT   STRAND          119..126
FT                   /evidence="ECO:0007829|PDB:1R9F"
FT   HELIX           127..129
FT                   /evidence="ECO:0007829|PDB:1R9F"
FT   HELIX           130..145
FT                   /evidence="ECO:0007829|PDB:1R9F"
SQ   SEQUENCE   172 AA;  19395 MW;  C6CDD00B5C90AB96 CRC64;
     MERAIQGNDA REQANSERWD GGSGGTTSPF KLPDESPSWT EWRLHNDETN SNQDNPLGFK
     ESWGFGKVVF KRYLRYDRTE ASLHRVLGSW TGDSVNYAAS RFFGFDQIGC TYSIRFRGVS
     ITVSGGSRTL QHLCEMAIRS KQELLQLAPI EVESNVSRGC PEGTETFEKE SE
//