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Protein

RNA silencing suppressor p19

Gene

ORF4

Organism
Tomato bushy stunt virus (strain Cherry) (TBSV)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. Binds to short interfering RNAs (siRNAs) with high affinity. Acts as a molecular caliper to specifically select siRNAs based on the length of the duplex region of the RNA (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Suppressor of RNA silencing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA silencing suppressor p19
Alternative name(s):
19 kDa symptom severity modulator
Gene namesi
ORF Names:ORF4
OrganismiTomato bushy stunt virus (strain Cherry) (TBSV)
Taxonomic identifieri12147 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageTombusviridaeTombusvirus
Virus hostiCapsicum annuum (Bell pepper) [TaxID: 4072]
Malus [TaxID: 3749]
Pyrus (pears) [TaxID: 3766]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]
Solanum melongena (Eggplant) (Aubergine) [TaxID: 4111]
Tolmiea menziesii [TaxID: 29777]
Tulipa [TaxID: 13305]
ProteomesiUP000001666 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 172172RNA silencing suppressor p19PRO_0000222881Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1
172
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi39 – 4810Combined sources
Beta strandi57 – 659Combined sources
Beta strandi68 – 758Combined sources
Helixi80 – 878Combined sources
Helixi92 – 1009Combined sources
Beta strandi109 – 1168Combined sources
Beta strandi119 – 1268Combined sources
Helixi127 – 1293Combined sources
Helixi130 – 14516Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R9FX-ray1.85A27-158[»]
ProteinModelPortaliP11690.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11690.

Family & Domainsi

Sequence similaritiesi

Belongs to the tombusviruses protein p19 family.Curated

Family and domain databases

InterProiIPR004905. Tombusvirus_p19.
[Graphical view]
PfamiPF03220. Tombus_P19. 1 hit.
[Graphical view]
ProDomiPD005743. Tombus_P19. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF103145. SSF103145. 1 hit.

Sequencei

Sequence statusi: Complete.

P11690-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERAIQGNDA REQANSERWD GGSGGTTSPF KLPDESPSWT EWRLHNDETN
60 70 80 90 100
SNQDNPLGFK ESWGFGKVVF KRYLRYDRTE ASLHRVLGSW TGDSVNYAAS
110 120 130 140 150
RFFGFDQIGC TYSIRFRGVS ITVSGGSRTL QHLCEMAIRS KQELLQLAPI
160 170
EVESNVSRGC PEGTETFEKE SE
Length:172
Mass (Da):19,395
Last modified:October 1, 1989 - v1
Checksum:iC6CDD00B5C90AB96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21958 Genomic RNA. Translation: AAB02538.1.
PIRiJA0116. NKVGTD.
RefSeqiNP_062901.1. NC_001554.1.

Genome annotation databases

GeneIDi1493957.
KEGGivg:1493957.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21958 Genomic RNA. Translation: AAB02538.1.
PIRiJA0116. NKVGTD.
RefSeqiNP_062901.1. NC_001554.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R9FX-ray1.85A27-158[»]
ProteinModelPortaliP11690.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1493957.
KEGGivg:1493957.

Miscellaneous databases

EvolutionaryTraceiP11690.

Family and domain databases

InterProiIPR004905. Tombusvirus_p19.
[Graphical view]
PfamiPF03220. Tombus_P19. 1 hit.
[Graphical view]
ProDomiPD005743. Tombus_P19. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF103145. SSF103145. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Organization of tomato bushy stunt virus genome: characterization of the coat protein gene and the 3' terminus."
    Hillman B.I., Hearne P.Q., Rochon D., Morris T.J.
    Virology 169:42-50(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "The complete genome structure and synthesis of infectious RNA from clones of tomato bushy stunt virus."
    Hearne P.Q., Knorr D.A., Hillman B.I., Morris T.J.
    Virology 177:141-151(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Suppression of gene silencing: a general strategy used by diverse DNA and RNA viruses of plants."
    Voinnet O., Pinto Y.M., Baulcombe D.C.
    Proc. Natl. Acad. Sci. U.S.A. 96:14147-14152(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Recognition of small interfering RNA by a viral suppressor of RNA silencing."
    Ye K., Malinina L., Patel D.J.
    Nature 426:874-878(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 23-158.

Entry informationi

Entry nameiP19_TBSVC
AccessioniPrimary (citable) accession number: P11690
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 24, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.