Integrin alpha-5 precursor - Mus musculus (Mouse)

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P11688 (ITA5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 128. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Integrin alpha-5

Alternative name(s):
CD49 antigen-like family member E
Fibronectin receptor subunit alpha
Integrin alpha-F
VLA-5
CD_antigen=CD49e

Gene names

Name:

Itga5

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1053 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. May play a role in the survival of adult skeletal muscle.
Subunit structure	Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with NISCH. Interacts with HPS5 By similarity. Interacts with RAB21 and COMP By similarity. Ref.5 Ref.6 Ref.7
Subcellular location	Membrane; Single-pass type I membrane protein.
Post-translational modification	Proteolytic cleavage by PCSK5 mediates activation of the precursor By similarity.
Disruption phenotype	Mice die at day E10-E11. They show both extraembryonic and embryonic vascular defects, and severe abnormalities in the development of the posterior trunk. Ref.4
Sequence similarities	Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Domain	Repeat Signal Transmembrane Transmembrane helix
Ligand	Calcium Metal-binding
Molecular function	Integrin Receptor
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell-cell adhesion mediated by integrin Inferred from mutant phenotype PubMed 8557754. Source: MGI cell-substrate junction assembly Inferred from direct assay PubMed 12867986. Source: MGI heterophilic cell-cell adhesion Inferred from mutant phenotype PubMed 8557754. Source: MGI integrin-mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW leukocyte cell-cell adhesion Inferred from mutant phenotype PubMed 8557754. Source: MGI memory Inferred from mutant phenotype PubMed 12904471. Source: MGI negative regulation of anoikis Inferred from electronic annotation. Source: Compara positive regulation of peptidyl-tyrosine phosphorylation Inferred from electronic annotation. Source: Compara wound healing, spreading of epidermal cells Inferred from electronic annotation. Source: Compara
Cellular_component	cell-cell junction Inferred from electronic annotation. Source: Compara external side of plasma membrane Inferred from direct assay PubMed 8557754. Source: MGI focal adhesion Inferred from electronic annotation. Source: Compara integrin complex Inferred from electronic annotation. Source: InterPro ruffle membrane Inferred from electronic annotation. Source: Compara synapse Inferred from direct assay PubMed 12904471. Source: MGI
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 44

By similarity

Chain

45 – 1053

1009

Integrin alpha-5

PRO_0000016252

Chain

45 – 898

854

Integrin alpha-5 heavy chain

PRO_0000016253

Chain

899 – 1053

155

Integrin alpha-5 light chain

PRO_0000016254

Regions

Topological domain

45 – 999

955

Extracellular Potential

Transmembrane

1000 – 1025

Helical; Potential

Topological domain

1026 – 1053

Cytoplasmic Potential

Repeat

46 – 111

FG-GAP 1

Repeat

131 – 191

FG-GAP 2

Repeat

196 – 248

FG-GAP 3

Repeat

262 – 318

FG-GAP 4

Repeat

319 – 380

FG-GAP 5

Repeat

381 – 440

FG-GAP 6

Repeat

444 – 507

FG-GAP 7

Calcium binding

283 – 291

By similarity

Calcium binding

337 – 345

Potential

Calcium binding

404 – 412

Potential

Calcium binding

468 – 476

Potential

Motif

1024 – 1028

GFFKR motif

Sites

Metal binding

283

Calcium 1 By similarity

Metal binding

285

Calcium 1 By similarity

Metal binding

287

Calcium 1 By similarity

Metal binding

289

Calcium 1; via carbonyl oxygen By similarity

Metal binding

291

Calcium 1 By similarity

Metal binding

337

Calcium 2 By similarity

Metal binding

339

Calcium 2 By similarity

Metal binding

341

Calcium 2 By similarity

Metal binding

343

Calcium 2; via carbonyl oxygen By similarity

Metal binding

345

Calcium 2 By similarity

Metal binding

404

Calcium 3 By similarity

Metal binding

406

Calcium 3 By similarity

Metal binding

408

Calcium 3 By similarity

Metal binding

410

Calcium 3; via carbonyl oxygen By similarity

Metal binding

412

Calcium 3 By similarity

Metal binding

468

Calcium 4 By similarity

Metal binding

470

Calcium 4 By similarity

Metal binding

472

Calcium 4 By similarity

Metal binding

474

Calcium 4; via carbonyl oxygen By similarity

Metal binding

476

Calcium 4 By similarity

Binding site

265

Arg of R-G-D substrate By similarity

Binding site

272

Arg of R-G-D substrate By similarity

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

185

N-linked (GlcNAc...) Ref.9

Glycosylation

300

N-linked (GlcNAc...) Ref.8

Glycosylation

310

N-linked (GlcNAc...) Ref.8

Glycosylation

319

N-linked (GlcNAc...) Potential

Glycosylation

527

N-linked (GlcNAc...) Potential

Glycosylation

533

N-linked (GlcNAc...) Potential

Glycosylation

596

N-linked (GlcNAc...) Ref.8 Ref.9

Glycosylation

612

N-linked (GlcNAc...) Ref.8 Ref.9

Glycosylation

675

N-linked (GlcNAc...) Ref.8

Glycosylation

678

N-linked (GlcNAc...) Ref.8 Ref.9

Glycosylation

685

N-linked (GlcNAc...) Ref.8

Glycosylation

715

N-linked (GlcNAc...) Ref.9

Glycosylation

727

N-linked (GlcNAc...) Ref.9

Glycosylation

776

N-linked (GlcNAc...) Potential

Glycosylation

872

N-linked (GlcNAc...) Potential

Glycosylation

876

N-linked (GlcNAc...) Ref.9

Disulfide bond

102 ↔ 111

By similarity

Disulfide bond

159 ↔ 179

By similarity

Disulfide bond

195 ↔ 208

By similarity

Disulfide bond

516 ↔ 525

By similarity

Disulfide bond

531 ↔ 587

By similarity

Disulfide bond

648 ↔ 654

By similarity

Disulfide bond

721 ↔ 734

By similarity

Disulfide bond

873 ↔ 915

Interchain (between heavy and light chains) By similarity

Disulfide bond

920 ↔ 925

By similarity

Experimental info

Sequence conflict

E → Q in CAA55638. Ref.1

Sequence conflict

377

G → A in CAA55638. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P11688 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 8624AEF83E4A9C07
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FASTA

1,053

115,043

        10         20         30         40         50         60 
MGSWTPRSPR SPLHAVLLRW GPRRLPPLLP LLLLLWPPPL QVGGFNLDAE APAVLSGPPG 

        70         80         90        100        110        120 
SLFGFSVEFY RPGRDGVSVL VGAPKANTSQ PGVLQGGAVY VCPWGTSPIQ CTTIQFDSKG 

       130        140        150        160        170        180 
SRILESSLYS AKGEEPVEYK SLQWFGATVR AHGSSILACA PLYSWRTEKD PQNDPVGTCY 

       190        200        210        220        230        240 
LSTENFTRIL EYAPCRSDFG SAAGQGYCQG GFSAEFTKTG RVVLGGPGSY FWQGQILSAT 

       250        260        270        280        290        300 
QEQISESYYP EYLINPVQGQ LQTRQASSVY DDSYLGYSVA VGEFSGDDTE DFVAGVPKGN 

       310        320        330        340        350        360 
LTYGYVTVLN GSDIHSLYNV SGEQMASYFG YAVAATDTNG DGLDDLLVGA PLLMERTADG 

       370        380        390        400        410        420 
RPQEVGRVYI YLQRPAGIDP TPTLTLTGQD EFSRFGSSLT PLGDLDQDGY NDVAIGAPFG 

       430        440        450        460        470        480 
GEAQQGVVFI FPGGPGGLST KPSQVLQPLW AAGRTPDFFG SALRGGRDLD GNGYPDLIVG 

       490        500        510        520        530        540 
SFGVDKALVY RGRPIISASA SLTIFPSMFN PEERSCSLEG NPVSCINLSF CLNASGKHVP 

       550        560        570        580        590        600 
NSIGFEVELQ LDWQKQKGGV RRALFLTSKQ ATLTQTLLIQ NGAREDCREM KIYLRNESEF 

       610        620        630        640        650        660 
RDKLSPIHIA LNFSLDPKAP MDSHGLRPVL HYQSKSRIED KAQILLDCGE DNICVPDLQL 

       670        680        690        700        710        720 
DVYGEKKHVY LGDKNALNLT FHAQNLGEGG AYEAELRVTA PLEAEYSGLV RHPGNFSSLS 

       730        740        750        760        770        780 
CDYFAVNQSR QLVCDLGNPM KAGTSLWGGL RFTVPHLQDT KKTIQFDFQI LSKNLNNSQS 

       790        800        810        820        830        840 
NVVSFPLSVE AQAQVSLNGV SKPEAVIFPV SDWNPQDQPQ KEEDLGPAVH HVYELINQGP 

       850        860        870        880        890        900 
SSISQGVLEL SCPQALEGQQ LLYVTKVTGL SNCTSNYTPN SQGLELDPET SPHHLQKREA 

       910        920        930        940        950        960 
PGRSSTASGT QVLKCPEAKC FRLRCEFGPL HRQESRSLQL HFRVWAKTFL QREYQPFSLQ 

       970        980        990       1000       1010       1020 
CEAVYEALKM PYQILPRQLP QKKLQVATAV QWTKAEGSNG VPLWIIILAI LFGLLLLGLL 

      1030       1040       1050 
IYVLYKLGFF KRSLPYGTAM EKAQLKPPAT SDA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Morrisey E., Dutt P., Patel V. Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Bone marrow.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Molecular cloning of a murine fibronectin receptor and its expression during inflammation. Expression of VLA-5 is increased in activated peritoneal macrophages in a manner discordant from major histocompatibility complex class II." Holers V.M., Ruff T.G., Parks D.L., McDonald J.A., Ballard L.L., Brown E.J. J. Exp. Med. 169:1589-1605(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 645-1053. Strain: BALB/c.
[4]	"Embryonic mesodermal defects in alpha 5 integrin-deficient mice." Yang J.T., Rayburn H., Hynes R.O. Development 119:1093-1105(1993) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[5]	"Nischarin, a novel protein that interacts with the integrin alpha5 subunit and inhibits cell migration." Alahari S.K., Lee J.W., Juliano R.L. J. Cell Biol. 151:1141-1154(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NISCH. Strain: ICR. Tissue: Brain.
[6]	"A membrane proximal region of the integrin alpha5 subunit is important for its interaction with nischarin." Alahari S.K., Nasrallah H. Biochem. J. 377:449-457(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NISCH.
[7]	"The integrin-binding protein Nischarin regulates cell migration by inhibiting PAK." Alahari S.K., Reddig P.J., Juliano R.L. EMBO J. 23:2777-2788(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NISCH.
[8]	"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation." Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B. Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-300; ASN-310; ASN-596; ASN-612; ASN-675; ASN-678 AND ASN-685, MASS SPECTROMETRY. Tissue: Myoblast.
[9]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-185; ASN-596; ASN-612; ASN-678; ASN-715; ASN-727 AND ASN-876, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X79003 mRNA. Translation: CAA55638.1. AC131721 Genomic DNA. No translation available. X15203 mRNA. Translation: CAA33273.1.
IPI	IPI00115976.
PIR	S44250.
RefSeq	NP_034707.3. NM_010577.3.
UniGene	Mm.16234.
3D structure databases
ProteinModelPortal	P11688.
SMR	P11688. Positions 45-1006.
ModBase	Search...
Protein-protein interaction databases
IntAct	P11688. 1 interaction.
STRING	10090.ENSMUSP00000023128.
PTM databases
PhosphoSite	P11688.
Proteomic databases
PaxDb	P11688.
PRIDE	P11688.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000023128; ENSMUSP00000023128; ENSMUSG00000000555.
GeneID	16402.
KEGG	mmu:16402.
Organism-specific databases
CTD	3678.
MGI	MGI:96604. Itga5.
Phylogenomic databases
eggNOG	NOG26407.
GeneTree	ENSGT00700000104007.
HOGENOM	HOG000231603.
HOVERGEN	HBG006186.
InParanoid	P11688.
KO	K06484.
OMA	ERSCSLE.
OrthoDB	EOG43JC3X.
Gene expression databases
ArrayExpress	P11688.
Bgee	P11688.
CleanEx	MM_ITGA5.
Genevestigator	P11688.
GermOnline	ENSMUSG00000000555. Mus musculus.
Family and domain databases
InterPro	IPR013517. FG-GAP. IPR013519. Int_alpha_beta-p. IPR000413. Integrin_alpha. IPR013649. Integrin_alpha-2. IPR018184. Integrin_alpha_C_CS. [Graphical view]
Pfam	PF01839. FG-GAP. 3 hits. PF08441. Integrin_alpha2. 1 hit. [Graphical view]
PRINTS	PR01185. INTEGRINA.
SMART	SM00191. Int_alpha. 5 hits. [Graphical view]
PROSITE	PS51470. FG_GAP. 7 hits. PS00242. INTEGRIN_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ITGA5. mouse.
NextBio	289561.
SOURCE	Search...

Entry information

Entry name

ITA5_MOUSE

Accession

Primary (citable) accession number: P11688
Secondary accession number(s): E9QN40

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 128 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following 2 chains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents