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P11688

- ITA5_MOUSE

UniProt

P11688 - ITA5_MOUSE

Protein

Integrin alpha-5

Gene

Itga5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. May play a role in the survival of adult skeletal muscle.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei265 – 2651Arg of R-G-D substrateBy similarity
    Binding sitei272 – 2721Arg of R-G-D substrateBy similarity
    Metal bindingi283 – 2831Calcium 1By similarity
    Metal bindingi285 – 2851Calcium 1By similarity
    Metal bindingi287 – 2871Calcium 1By similarity
    Metal bindingi289 – 2891Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi291 – 2911Calcium 1By similarity
    Metal bindingi337 – 3371Calcium 2By similarity
    Metal bindingi339 – 3391Calcium 2By similarity
    Metal bindingi341 – 3411Calcium 2By similarity
    Metal bindingi343 – 3431Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi345 – 3451Calcium 2By similarity
    Metal bindingi404 – 4041Calcium 3By similarity
    Metal bindingi406 – 4061Calcium 3By similarity
    Metal bindingi408 – 4081Calcium 3By similarity
    Metal bindingi410 – 4101Calcium 3; via carbonyl oxygenBy similarity
    Metal bindingi412 – 4121Calcium 3By similarity
    Metal bindingi468 – 4681Calcium 4By similarity
    Metal bindingi470 – 4701Calcium 4By similarity
    Metal bindingi472 – 4721Calcium 4By similarity
    Metal bindingi474 – 4741Calcium 4; via carbonyl oxygenBy similarity
    Metal bindingi476 – 4761Calcium 4By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi283 – 2919By similarity
    Calcium bindingi337 – 3459Sequence Analysis
    Calcium bindingi404 – 4129Sequence Analysis
    Calcium bindingi468 – 4769Sequence Analysis

    GO - Molecular functioni

    1. integrin binding Source: MGI
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: MGI

    GO - Biological processi

    1. cell-cell adhesion mediated by integrin Source: MGI
    2. cell-substrate junction assembly Source: MGI
    3. heterophilic cell-cell adhesion Source: MGI
    4. integrin-mediated signaling pathway Source: UniProtKB-KW
    5. leukocyte cell-cell adhesion Source: MGI
    6. memory Source: MGI
    7. negative regulation of anoikis Source: Ensembl
    8. positive regulation of cell migration Source: Ensembl
    9. positive regulation of cell-substrate adhesion Source: Ensembl
    10. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
    11. wound healing, spreading of epidermal cells Source: Ensembl

    Keywords - Molecular functioni

    Integrin, Receptor

    Keywords - Biological processi

    Cell adhesion

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_198996. Elastic fibre formation.
    REACT_204211. Fibronectin matrix formation.
    REACT_215461. Signal transduction by L1.
    REACT_216309. Integrin cell surface interactions.
    REACT_225233. Cell surface interactions at the vascular wall.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Integrin alpha-5
    Alternative name(s):
    CD49 antigen-like family member E
    Fibronectin receptor subunit alpha
    Integrin alpha-F
    VLA-5
    CD_antigen: CD49e
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Itga5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:96604. Itga5.

    Subcellular locationi

    GO - Cellular componenti

    1. cell-cell junction Source: Ensembl
    2. cell surface Source: MGI
    3. cytoplasm Source: Ensembl
    4. external side of plasma membrane Source: MGI
    5. focal adhesion Source: Ensembl
    6. integrin complex Source: Ensembl
    7. ruffle membrane Source: Ensembl
    8. synapse Source: MGI

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice die at day E10-E11. They show both extraembryonic and embryonic vascular defects, and severe abnormalities in the development of the posterior trunk.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4444By similarityAdd
    BLAST
    Chaini45 – 10531009Integrin alpha-5PRO_0000016252Add
    BLAST
    Chaini45 – 898854Integrin alpha-5 heavy chainPRO_0000016253Add
    BLAST
    Chaini899 – 1053155Integrin alpha-5 light chainPRO_0000016254Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi87 – 871N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi102 ↔ 111By similarity
    Disulfide bondi159 ↔ 179By similarity
    Glycosylationi185 – 1851N-linked (GlcNAc...)1 Publication
    Disulfide bondi195 ↔ 208By similarity
    Glycosylationi300 – 3001N-linked (GlcNAc...)1 Publication
    Glycosylationi310 – 3101N-linked (GlcNAc...)1 Publication
    Glycosylationi319 – 3191N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi516 ↔ 525By similarity
    Glycosylationi527 – 5271N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi531 ↔ 587By similarity
    Glycosylationi533 – 5331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi596 – 5961N-linked (GlcNAc...)2 Publications
    Glycosylationi612 – 6121N-linked (GlcNAc...)2 Publications
    Disulfide bondi648 ↔ 654By similarity
    Glycosylationi675 – 6751N-linked (GlcNAc...); atypical1 Publication
    Glycosylationi678 – 6781N-linked (GlcNAc...)2 Publications
    Glycosylationi685 – 6851N-linked (GlcNAc...); atypical1 Publication
    Glycosylationi715 – 7151N-linked (GlcNAc...)1 Publication
    Disulfide bondi721 ↔ 734By similarity
    Glycosylationi727 – 7271N-linked (GlcNAc...)1 Publication
    Glycosylationi776 – 7761N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi872 – 8721N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi873 ↔ 915Interchain (between heavy and light chains)By similarity
    Glycosylationi876 – 8761N-linked (GlcNAc...)1 Publication
    Disulfide bondi920 ↔ 925By similarity

    Post-translational modificationi

    Proteolytic cleavage by PCSK5 mediates activation of the precursor.By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP11688.
    PRIDEiP11688.

    PTM databases

    PhosphoSiteiP11688.

    Expressioni

    Gene expression databases

    ArrayExpressiP11688.
    BgeeiP11688.
    CleanExiMM_ITGA5.
    GenevestigatoriP11688.

    Interactioni

    Subunit structurei

    Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with NISCH. Interacts with HPS5 By similarity. Interacts with RAB21 and COMP. Interacts with CIB1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi200818. 3 interactions.
    IntActiP11688. 3 interactions.
    MINTiMINT-4098865.
    STRINGi10090.ENSMUSP00000023128.

    Structurei

    3D structure databases

    ProteinModelPortaliP11688.
    SMRiP11688. Positions 45-992, 1001-1036.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini45 – 999955ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1026 – 105328CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1000 – 102526HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati46 – 11166FG-GAP 1Add
    BLAST
    Repeati131 – 19161FG-GAP 2Add
    BLAST
    Repeati196 – 24853FG-GAP 3Add
    BLAST
    Repeati262 – 31857FG-GAP 4Add
    BLAST
    Repeati319 – 38062FG-GAP 5Add
    BLAST
    Repeati381 – 44060FG-GAP 6Add
    BLAST
    Repeati444 – 50764FG-GAP 7Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1024 – 10285GFFKR motif

    Sequence similaritiesi

    Belongs to the integrin alpha chain family.Curated
    Contains 7 FG-GAP repeats.Curated

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG26407.
    GeneTreeiENSGT00750000117267.
    HOGENOMiHOG000231603.
    HOVERGENiHBG006186.
    InParanoidiP11688.
    KOiK06484.
    OMAiERSCSLE.
    OrthoDBiEOG7HQN77.
    TreeFamiTF105391.

    Family and domain databases

    InterProiIPR013517. FG-GAP.
    IPR013519. Int_alpha_beta-p.
    IPR000413. Integrin_alpha.
    IPR013649. Integrin_alpha-2.
    IPR018184. Integrin_alpha_C_CS.
    [Graphical view]
    PfamiPF01839. FG-GAP. 3 hits.
    PF08441. Integrin_alpha2. 1 hit.
    [Graphical view]
    PRINTSiPR01185. INTEGRINA.
    SMARTiSM00191. Int_alpha. 5 hits.
    [Graphical view]
    PROSITEiPS51470. FG_GAP. 7 hits.
    PS00242. INTEGRIN_ALPHA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P11688-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSWTPRSPR SPLHAVLLRW GPRRLPPLLP LLLLLWPPPL QVGGFNLDAE     50
    APAVLSGPPG SLFGFSVEFY RPGRDGVSVL VGAPKANTSQ PGVLQGGAVY 100
    VCPWGTSPIQ CTTIQFDSKG SRILESSLYS AKGEEPVEYK SLQWFGATVR 150
    AHGSSILACA PLYSWRTEKD PQNDPVGTCY LSTENFTRIL EYAPCRSDFG 200
    SAAGQGYCQG GFSAEFTKTG RVVLGGPGSY FWQGQILSAT QEQISESYYP 250
    EYLINPVQGQ LQTRQASSVY DDSYLGYSVA VGEFSGDDTE DFVAGVPKGN 300
    LTYGYVTVLN GSDIHSLYNV SGEQMASYFG YAVAATDTNG DGLDDLLVGA 350
    PLLMERTADG RPQEVGRVYI YLQRPAGIDP TPTLTLTGQD EFSRFGSSLT 400
    PLGDLDQDGY NDVAIGAPFG GEAQQGVVFI FPGGPGGLST KPSQVLQPLW 450
    AAGRTPDFFG SALRGGRDLD GNGYPDLIVG SFGVDKALVY RGRPIISASA 500
    SLTIFPSMFN PEERSCSLEG NPVSCINLSF CLNASGKHVP NSIGFEVELQ 550
    LDWQKQKGGV RRALFLTSKQ ATLTQTLLIQ NGAREDCREM KIYLRNESEF 600
    RDKLSPIHIA LNFSLDPKAP MDSHGLRPVL HYQSKSRIED KAQILLDCGE 650
    DNICVPDLQL DVYGEKKHVY LGDKNALNLT FHAQNLGEGG AYEAELRVTA 700
    PLEAEYSGLV RHPGNFSSLS CDYFAVNQSR QLVCDLGNPM KAGTSLWGGL 750
    RFTVPHLQDT KKTIQFDFQI LSKNLNNSQS NVVSFPLSVE AQAQVSLNGV 800
    SKPEAVIFPV SDWNPQDQPQ KEEDLGPAVH HVYELINQGP SSISQGVLEL 850
    SCPQALEGQQ LLYVTKVTGL SNCTSNYTPN SQGLELDPET SPHHLQKREA 900
    PGRSSTASGT QVLKCPEAKC FRLRCEFGPL HRQESRSLQL HFRVWAKTFL 950
    QREYQPFSLQ CEAVYEALKM PYQILPRQLP QKKLQVATAV QWTKAEGSNG 1000
    VPLWIIILAI LFGLLLLGLL IYVLYKLGFF KRSLPYGTAM EKAQLKPPAT 1050
    SDA 1053
    Length:1,053
    Mass (Da):115,043
    Last modified:July 27, 2011 - v3
    Checksum:i8624AEF83E4A9C07
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti68 – 681E → Q in CAA55638. 1 PublicationCurated
    Sequence conflicti377 – 3771G → A in CAA55638. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79003 mRNA. Translation: CAA55638.1.
    AC131721 Genomic DNA. No translation available.
    X15203 mRNA. Translation: CAA33273.1.
    CCDSiCCDS27903.1.
    PIRiS44250.
    RefSeqiNP_034707.3. NM_010577.3.
    UniGeneiMm.16234.

    Genome annotation databases

    EnsembliENSMUST00000023128; ENSMUSP00000023128; ENSMUSG00000000555.
    GeneIDi16402.
    KEGGimmu:16402.
    UCSCiuc007xyb.3. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79003 mRNA. Translation: CAA55638.1 .
    AC131721 Genomic DNA. No translation available.
    X15203 mRNA. Translation: CAA33273.1 .
    CCDSi CCDS27903.1.
    PIRi S44250.
    RefSeqi NP_034707.3. NM_010577.3.
    UniGenei Mm.16234.

    3D structure databases

    ProteinModelPortali P11688.
    SMRi P11688. Positions 45-992, 1001-1036.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200818. 3 interactions.
    IntActi P11688. 3 interactions.
    MINTi MINT-4098865.
    STRINGi 10090.ENSMUSP00000023128.

    PTM databases

    PhosphoSitei P11688.

    Proteomic databases

    PaxDbi P11688.
    PRIDEi P11688.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023128 ; ENSMUSP00000023128 ; ENSMUSG00000000555 .
    GeneIDi 16402.
    KEGGi mmu:16402.
    UCSCi uc007xyb.3. mouse.

    Organism-specific databases

    CTDi 3678.
    MGIi MGI:96604. Itga5.

    Phylogenomic databases

    eggNOGi NOG26407.
    GeneTreei ENSGT00750000117267.
    HOGENOMi HOG000231603.
    HOVERGENi HBG006186.
    InParanoidi P11688.
    KOi K06484.
    OMAi ERSCSLE.
    OrthoDBi EOG7HQN77.
    TreeFami TF105391.

    Enzyme and pathway databases

    Reactomei REACT_198996. Elastic fibre formation.
    REACT_204211. Fibronectin matrix formation.
    REACT_215461. Signal transduction by L1.
    REACT_216309. Integrin cell surface interactions.
    REACT_225233. Cell surface interactions at the vascular wall.

    Miscellaneous databases

    ChiTaRSi ITGA5. mouse.
    NextBioi 289561.
    PROi P11688.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P11688.
    Bgeei P11688.
    CleanExi MM_ITGA5.
    Genevestigatori P11688.

    Family and domain databases

    InterProi IPR013517. FG-GAP.
    IPR013519. Int_alpha_beta-p.
    IPR000413. Integrin_alpha.
    IPR013649. Integrin_alpha-2.
    IPR018184. Integrin_alpha_C_CS.
    [Graphical view ]
    Pfami PF01839. FG-GAP. 3 hits.
    PF08441. Integrin_alpha2. 1 hit.
    [Graphical view ]
    PRINTSi PR01185. INTEGRINA.
    SMARTi SM00191. Int_alpha. 5 hits.
    [Graphical view ]
    PROSITEi PS51470. FG_GAP. 7 hits.
    PS00242. INTEGRIN_ALPHA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Morrisey E., Dutt P., Patel V.
      Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Bone marrow.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "Molecular cloning of a murine fibronectin receptor and its expression during inflammation. Expression of VLA-5 is increased in activated peritoneal macrophages in a manner discordant from major histocompatibility complex class II."
      Holers V.M., Ruff T.G., Parks D.L., McDonald J.A., Ballard L.L., Brown E.J.
      J. Exp. Med. 169:1589-1605(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 645-1053.
      Strain: BALB/c.
    4. "Embryonic mesodermal defects in alpha 5 integrin-deficient mice."
      Yang J.T., Rayburn H., Hynes R.O.
      Development 119:1093-1105(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    5. "Nischarin, a novel protein that interacts with the integrin alpha5 subunit and inhibits cell migration."
      Alahari S.K., Lee J.W., Juliano R.L.
      J. Cell Biol. 151:1141-1154(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NISCH.
      Strain: ICR.
      Tissue: Brain.
    6. "A membrane proximal region of the integrin alpha5 subunit is important for its interaction with nischarin."
      Alahari S.K., Nasrallah H.
      Biochem. J. 377:449-457(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NISCH.
    7. "The integrin-binding protein Nischarin regulates cell migration by inhibiting PAK."
      Alahari S.K., Reddig P.J., Juliano R.L.
      EMBO J. 23:2777-2788(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NISCH.
    8. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
      Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
      Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-300; ASN-310; ASN-596; ASN-612; ASN-675; ASN-678 AND ASN-685.
      Tissue: Myoblast.
    9. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-185; ASN-596; ASN-612; ASN-678; ASN-715; ASN-727 AND ASN-876.

    Entry informationi

    Entry nameiITA5_MOUSE
    AccessioniPrimary (citable) accession number: P11688
    Secondary accession number(s): E9QN40
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 142 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3