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P11688

- ITA5_MOUSE

UniProt

P11688 - ITA5_MOUSE

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Protein
Integrin alpha-5
Gene
Itga5
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. May play a role in the survival of adult skeletal muscle.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei265 – 2651Arg of R-G-D substrate By similarity
Binding sitei272 – 2721Arg of R-G-D substrate By similarity
Metal bindingi283 – 2831Calcium 1 By similarity
Metal bindingi285 – 2851Calcium 1 By similarity
Metal bindingi287 – 2871Calcium 1 By similarity
Metal bindingi289 – 2891Calcium 1; via carbonyl oxygen By similarity
Metal bindingi291 – 2911Calcium 1 By similarity
Metal bindingi337 – 3371Calcium 2 By similarity
Metal bindingi339 – 3391Calcium 2 By similarity
Metal bindingi341 – 3411Calcium 2 By similarity
Metal bindingi343 – 3431Calcium 2; via carbonyl oxygen By similarity
Metal bindingi345 – 3451Calcium 2 By similarity
Metal bindingi404 – 4041Calcium 3 By similarity
Metal bindingi406 – 4061Calcium 3 By similarity
Metal bindingi408 – 4081Calcium 3 By similarity
Metal bindingi410 – 4101Calcium 3; via carbonyl oxygen By similarity
Metal bindingi412 – 4121Calcium 3 By similarity
Metal bindingi468 – 4681Calcium 4 By similarity
Metal bindingi470 – 4701Calcium 4 By similarity
Metal bindingi472 – 4721Calcium 4 By similarity
Metal bindingi474 – 4741Calcium 4; via carbonyl oxygen By similarity
Metal bindingi476 – 4761Calcium 4 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi283 – 2919 By similarity
Calcium bindingi337 – 3459 Reviewed prediction
Calcium bindingi404 – 4129 Reviewed prediction
Calcium bindingi468 – 4769 Reviewed prediction

GO - Molecular functioni

  1. integrin binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. protein binding Source: MGI

GO - Biological processi

  1. cell-cell adhesion mediated by integrin Source: MGI
  2. cell-substrate junction assembly Source: MGI
  3. heterophilic cell-cell adhesion Source: MGI
  4. integrin-mediated signaling pathway Source: UniProtKB-KW
  5. leukocyte cell-cell adhesion Source: MGI
  6. memory Source: MGI
  7. negative regulation of anoikis Source: Ensembl
  8. positive regulation of cell migration Source: Ensembl
  9. positive regulation of cell-substrate adhesion Source: Ensembl
  10. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  11. wound healing, spreading of epidermal cells Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Integrin, Receptor

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198996. Elastic fibre formation.
REACT_204211. Fibronectin matrix formation.
REACT_215461. Signal transduction by L1.
REACT_216309. Integrin cell surface interactions.
REACT_225233. Cell surface interactions at the vascular wall.

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin alpha-5
Alternative name(s):
CD49 antigen-like family member E
Fibronectin receptor subunit alpha
Integrin alpha-F
VLA-5
CD_antigen: CD49e
Cleaved into the following 2 chains:
Gene namesi
Name:Itga5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:96604. Itga5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini45 – 999955Extracellular Reviewed prediction
Add
BLAST
Transmembranei1000 – 102526Helical; Reviewed prediction
Add
BLAST
Topological domaini1026 – 105328Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cell surface Source: MGI
  2. cell-cell junction Source: Ensembl
  3. cytoplasm Source: Ensembl
  4. external side of plasma membrane Source: MGI
  5. focal adhesion Source: Ensembl
  6. integrin complex Source: Ensembl
  7. ruffle membrane Source: Ensembl
  8. synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice die at day E10-E11. They show both extraembryonic and embryonic vascular defects, and severe abnormalities in the development of the posterior trunk.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4444 By similarity
Add
BLAST
Chaini45 – 10531009Integrin alpha-5
PRO_0000016252Add
BLAST
Chaini45 – 898854Integrin alpha-5 heavy chain
PRO_0000016253Add
BLAST
Chaini899 – 1053155Integrin alpha-5 light chain
PRO_0000016254Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi87 – 871N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi102 ↔ 111 By similarity
Disulfide bondi159 ↔ 179 By similarity
Glycosylationi185 – 1851N-linked (GlcNAc...)1 Publication
Disulfide bondi195 ↔ 208 By similarity
Glycosylationi300 – 3001N-linked (GlcNAc...)1 Publication
Glycosylationi310 – 3101N-linked (GlcNAc...)1 Publication
Glycosylationi319 – 3191N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi516 ↔ 525 By similarity
Glycosylationi527 – 5271N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi531 ↔ 587 By similarity
Glycosylationi533 – 5331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi596 – 5961N-linked (GlcNAc...)2 Publications
Glycosylationi612 – 6121N-linked (GlcNAc...)2 Publications
Disulfide bondi648 ↔ 654 By similarity
Glycosylationi675 – 6751N-linked (GlcNAc...); atypical1 Publication
Glycosylationi678 – 6781N-linked (GlcNAc...)2 Publications
Glycosylationi685 – 6851N-linked (GlcNAc...); atypical1 Publication
Glycosylationi715 – 7151N-linked (GlcNAc...)1 Publication
Disulfide bondi721 ↔ 734 By similarity
Glycosylationi727 – 7271N-linked (GlcNAc...)1 Publication
Glycosylationi776 – 7761N-linked (GlcNAc...) Reviewed prediction
Glycosylationi872 – 8721N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi873 ↔ 915Interchain (between heavy and light chains) By similarity
Glycosylationi876 – 8761N-linked (GlcNAc...)1 Publication
Disulfide bondi920 ↔ 925 By similarity

Post-translational modificationi

Proteolytic cleavage by PCSK5 mediates activation of the precursor By similarity.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP11688.
PRIDEiP11688.

PTM databases

PhosphoSiteiP11688.

Expressioni

Gene expression databases

ArrayExpressiP11688.
BgeeiP11688.
CleanExiMM_ITGA5.
GenevestigatoriP11688.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with NISCH. Interacts with HPS5 By similarity. Interacts with RAB21 and COMP. Interacts with CIB1 By similarity.3 Publications

Protein-protein interaction databases

BioGridi200818. 3 interactions.
IntActiP11688. 3 interactions.
MINTiMINT-4098865.
STRINGi10090.ENSMUSP00000023128.

Structurei

3D structure databases

ProteinModelPortaliP11688.
SMRiP11688. Positions 45-992, 1001-1036.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati46 – 11166FG-GAP 1
Add
BLAST
Repeati131 – 19161FG-GAP 2
Add
BLAST
Repeati196 – 24853FG-GAP 3
Add
BLAST
Repeati262 – 31857FG-GAP 4
Add
BLAST
Repeati319 – 38062FG-GAP 5
Add
BLAST
Repeati381 – 44060FG-GAP 6
Add
BLAST
Repeati444 – 50764FG-GAP 7
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1024 – 10285GFFKR motif

Sequence similaritiesi

Contains 7 FG-GAP repeats.

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG26407.
GeneTreeiENSGT00750000117267.
HOGENOMiHOG000231603.
HOVERGENiHBG006186.
InParanoidiP11688.
KOiK06484.
OMAiERSCSLE.
OrthoDBiEOG7HQN77.
TreeFamiTF105391.

Family and domain databases

InterProiIPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
[Graphical view]
PfamiPF01839. FG-GAP. 3 hits.
PF08441. Integrin_alpha2. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 5 hits.
[Graphical view]
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11688-1 [UniParc]FASTAAdd to Basket

« Hide

MGSWTPRSPR SPLHAVLLRW GPRRLPPLLP LLLLLWPPPL QVGGFNLDAE     50
APAVLSGPPG SLFGFSVEFY RPGRDGVSVL VGAPKANTSQ PGVLQGGAVY 100
VCPWGTSPIQ CTTIQFDSKG SRILESSLYS AKGEEPVEYK SLQWFGATVR 150
AHGSSILACA PLYSWRTEKD PQNDPVGTCY LSTENFTRIL EYAPCRSDFG 200
SAAGQGYCQG GFSAEFTKTG RVVLGGPGSY FWQGQILSAT QEQISESYYP 250
EYLINPVQGQ LQTRQASSVY DDSYLGYSVA VGEFSGDDTE DFVAGVPKGN 300
LTYGYVTVLN GSDIHSLYNV SGEQMASYFG YAVAATDTNG DGLDDLLVGA 350
PLLMERTADG RPQEVGRVYI YLQRPAGIDP TPTLTLTGQD EFSRFGSSLT 400
PLGDLDQDGY NDVAIGAPFG GEAQQGVVFI FPGGPGGLST KPSQVLQPLW 450
AAGRTPDFFG SALRGGRDLD GNGYPDLIVG SFGVDKALVY RGRPIISASA 500
SLTIFPSMFN PEERSCSLEG NPVSCINLSF CLNASGKHVP NSIGFEVELQ 550
LDWQKQKGGV RRALFLTSKQ ATLTQTLLIQ NGAREDCREM KIYLRNESEF 600
RDKLSPIHIA LNFSLDPKAP MDSHGLRPVL HYQSKSRIED KAQILLDCGE 650
DNICVPDLQL DVYGEKKHVY LGDKNALNLT FHAQNLGEGG AYEAELRVTA 700
PLEAEYSGLV RHPGNFSSLS CDYFAVNQSR QLVCDLGNPM KAGTSLWGGL 750
RFTVPHLQDT KKTIQFDFQI LSKNLNNSQS NVVSFPLSVE AQAQVSLNGV 800
SKPEAVIFPV SDWNPQDQPQ KEEDLGPAVH HVYELINQGP SSISQGVLEL 850
SCPQALEGQQ LLYVTKVTGL SNCTSNYTPN SQGLELDPET SPHHLQKREA 900
PGRSSTASGT QVLKCPEAKC FRLRCEFGPL HRQESRSLQL HFRVWAKTFL 950
QREYQPFSLQ CEAVYEALKM PYQILPRQLP QKKLQVATAV QWTKAEGSNG 1000
VPLWIIILAI LFGLLLLGLL IYVLYKLGFF KRSLPYGTAM EKAQLKPPAT 1050
SDA 1053
Length:1,053
Mass (Da):115,043
Last modified:July 27, 2011 - v3
Checksum:i8624AEF83E4A9C07
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681E → Q in CAA55638. 1 Publication
Sequence conflicti377 – 3771G → A in CAA55638. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X79003 mRNA. Translation: CAA55638.1.
AC131721 Genomic DNA. No translation available.
X15203 mRNA. Translation: CAA33273.1.
CCDSiCCDS27903.1.
PIRiS44250.
RefSeqiNP_034707.3. NM_010577.3.
UniGeneiMm.16234.

Genome annotation databases

EnsembliENSMUST00000023128; ENSMUSP00000023128; ENSMUSG00000000555.
GeneIDi16402.
KEGGimmu:16402.
UCSCiuc007xyb.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X79003 mRNA. Translation: CAA55638.1 .
AC131721 Genomic DNA. No translation available.
X15203 mRNA. Translation: CAA33273.1 .
CCDSi CCDS27903.1.
PIRi S44250.
RefSeqi NP_034707.3. NM_010577.3.
UniGenei Mm.16234.

3D structure databases

ProteinModelPortali P11688.
SMRi P11688. Positions 45-992, 1001-1036.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200818. 3 interactions.
IntActi P11688. 3 interactions.
MINTi MINT-4098865.
STRINGi 10090.ENSMUSP00000023128.

PTM databases

PhosphoSitei P11688.

Proteomic databases

PaxDbi P11688.
PRIDEi P11688.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000023128 ; ENSMUSP00000023128 ; ENSMUSG00000000555 .
GeneIDi 16402.
KEGGi mmu:16402.
UCSCi uc007xyb.3. mouse.

Organism-specific databases

CTDi 3678.
MGIi MGI:96604. Itga5.

Phylogenomic databases

eggNOGi NOG26407.
GeneTreei ENSGT00750000117267.
HOGENOMi HOG000231603.
HOVERGENi HBG006186.
InParanoidi P11688.
KOi K06484.
OMAi ERSCSLE.
OrthoDBi EOG7HQN77.
TreeFami TF105391.

Enzyme and pathway databases

Reactomei REACT_198996. Elastic fibre formation.
REACT_204211. Fibronectin matrix formation.
REACT_215461. Signal transduction by L1.
REACT_216309. Integrin cell surface interactions.
REACT_225233. Cell surface interactions at the vascular wall.

Miscellaneous databases

ChiTaRSi ITGA5. mouse.
NextBioi 289561.
PROi P11688.
SOURCEi Search...

Gene expression databases

ArrayExpressi P11688.
Bgeei P11688.
CleanExi MM_ITGA5.
Genevestigatori P11688.

Family and domain databases

InterProi IPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
[Graphical view ]
Pfami PF01839. FG-GAP. 3 hits.
PF08441. Integrin_alpha2. 1 hit.
[Graphical view ]
PRINTSi PR01185. INTEGRINA.
SMARTi SM00191. Int_alpha. 5 hits.
[Graphical view ]
PROSITEi PS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Morrisey E., Dutt P., Patel V.
    Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Bone marrow.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Molecular cloning of a murine fibronectin receptor and its expression during inflammation. Expression of VLA-5 is increased in activated peritoneal macrophages in a manner discordant from major histocompatibility complex class II."
    Holers V.M., Ruff T.G., Parks D.L., McDonald J.A., Ballard L.L., Brown E.J.
    J. Exp. Med. 169:1589-1605(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 645-1053.
    Strain: BALB/c.
  4. "Embryonic mesodermal defects in alpha 5 integrin-deficient mice."
    Yang J.T., Rayburn H., Hynes R.O.
    Development 119:1093-1105(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  5. "Nischarin, a novel protein that interacts with the integrin alpha5 subunit and inhibits cell migration."
    Alahari S.K., Lee J.W., Juliano R.L.
    J. Cell Biol. 151:1141-1154(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NISCH.
    Strain: ICR.
    Tissue: Brain.
  6. "A membrane proximal region of the integrin alpha5 subunit is important for its interaction with nischarin."
    Alahari S.K., Nasrallah H.
    Biochem. J. 377:449-457(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NISCH.
  7. "The integrin-binding protein Nischarin regulates cell migration by inhibiting PAK."
    Alahari S.K., Reddig P.J., Juliano R.L.
    EMBO J. 23:2777-2788(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NISCH.
  8. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-300; ASN-310; ASN-596; ASN-612; ASN-675; ASN-678 AND ASN-685.
    Tissue: Myoblast.
  9. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-185; ASN-596; ASN-612; ASN-678; ASN-715; ASN-727 AND ASN-876.

Entry informationi

Entry nameiITA5_MOUSE
AccessioniPrimary (citable) accession number: P11688
Secondary accession number(s): E9QN40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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