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Protein

Keratin, type II cytoskeletal 8

Gene

Krt8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei347Stutter1

GO - Molecular functioni

GO - Biological processi

  • cell differentiation involved in embryonic placenta development Source: MGI
  • cell morphogenesis involved in differentiation Source: UniProtKB
  • extrinsic apoptotic signaling pathway Source: MGI
  • hepatocyte apoptotic process Source: MGI
  • multicellular organism development Source: UniProtKB
  • response to hydrostatic pressure Source: Ensembl
  • response to other organism Source: MGI
  • sarcomere organization Source: Ensembl
  • tumor necrosis factor-mediated signaling pathway Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-6805567. Keratinization.
R-MMU-6809371. Formation of the cornified envelope.

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type II cytoskeletal 8
Alternative name(s):
Cytokeratin endo A
Cytokeratin-8
Short name:
CK-8
Keratin-8
Short name:
K8
Type-II keratin Kb8
Gene namesi
Name:Krt8
Synonyms:Krt2-8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:96705. Krt8.

Subcellular locationi

GO - Cellular componenti

  • apicolateral plasma membrane Source: UniProtKB
  • cell-cell junction Source: MGI
  • cell periphery Source: MGI
  • costamere Source: Ensembl
  • cytoplasm Source: MGI
  • dystrophin-associated glycoprotein complex Source: Ensembl
  • extracellular exosome Source: MGI
  • intermediate filament Source: MGI
  • keratin filament Source: Ensembl
  • nuclear matrix Source: UniProtKB-SubCell
  • nucleoplasm Source: UniProtKB-SubCell
  • nucleus Source: MGI
  • sarcolemma Source: MGI
  • Z disc Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Intermediate filament, Keratin, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000637411 – 490Keratin, type II cytoskeletal 8Add BLAST490

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9Phosphoserine; by PKC/PRKCEBy similarity1
Modified residuei13PhosphoserineBy similarity1
Modified residuei15PhosphoserineBy similarity1
Modified residuei21PhosphoserineCombined sources1
Modified residuei22PhosphoserineBy similarity1
Modified residuei23Omega-N-methylarginineBy similarity1
Modified residuei24Phosphoserine; by PKC/PRKCECurated1
Modified residuei26PhosphothreonineBy similarity1
Modified residuei27PhosphoserineBy similarity1
Modified residuei32Omega-N-methylarginineBy similarity1
Modified residuei34PhosphoserineBy similarity1
Modified residuei37PhosphoserineBy similarity1
Modified residuei39PhosphoserineBy similarity1
Modified residuei40Omega-N-methylarginineBy similarity1
Modified residuei43PhosphoserineBy similarity1
Modified residuei44PhosphoserineBy similarity1
Modified residuei47PhosphoserineBy similarity1
Modified residuei49Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei49Omega-N-methylarginine; alternateBy similarity1
Modified residuei51PhosphoserineBy similarity1
Modified residuei80Phosphoserine; by MAPK1 Publication1
Modified residuei107N6-malonyllysineBy similarity1
Cross-linki203Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki203Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei213N6-acetyllysineBy similarity1
Modified residuei259PhosphoserineCombined sources1
Modified residuei280PhosphoserineBy similarity1
Cross-linki291Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei301N6-acetyllysineBy similarity1
Cross-linki310Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei331N6-acetyllysineBy similarity1
Modified residuei336PhosphoserineBy similarity1
Modified residuei406PhosphoserineBy similarity1
Modified residuei410PhosphoserineBy similarity1
Modified residuei416PhosphoserineBy similarity1
Modified residuei423PhosphoserineBy similarity1
Modified residuei430PhosphoserineBy similarity1
Modified residuei432PhosphoserineBy similarity1
Modified residuei438PhosphoserineBy similarity1
Cross-linki479Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki479Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei482PhosphoserineCombined sources1
Modified residuei484PhosphoserineBy similarity1
Modified residuei485PhosphoserineCombined sources1
Modified residuei489PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-80 phosphorylation plays an important role in keratin filament reorganization (By similarity).By similarity
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation (By similarity).By similarity
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP11679.
PaxDbiP11679.
PeptideAtlasiP11679.
PRIDEiP11679.

2D gel databases

SWISS-2DPAGEP11679.

PTM databases

iPTMnetiP11679.
PhosphoSitePlusiP11679.

Expressioni

Tissue specificityi

Expressed in abundance in the epithelia of colon, bladder, ileum, and stomach, with lower expression observed in earskin (at protein level). Also expressed in pancreas, liver, dudenum and jejunum.1 Publication

Gene expression databases

BgeeiENSMUSG00000049382.
CleanExiMM_KRT8.
GenevisibleiP11679. MM.

Interactioni

Subunit structurei

Heterotetramer of two type I and two type II keratins. KRT8 associates with KRT18. Associates with KRT20. Interacts with PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP. Interacts with APEX1 (By similarity). Interacts with GPER1 (By similarity). Interacts with EPPK1 (PubMed:25617501).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201037. 7 interactors.
IntActiP11679. 3 interactors.
MINTiMINT-1859050.
STRINGi10090.ENSMUSP00000023952.

Structurei

3D structure databases

ProteinModelPortaliP11679.
SMRiP11679.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 96HeadAdd BLAST96
Regioni97 – 403RodAdd BLAST307
Regioni97 – 132Coil 1AAdd BLAST36
Regioni133 – 149Linker 1Add BLAST17
Regioni150 – 241Coil 1BAdd BLAST92
Regioni242 – 265Linker 12Add BLAST24
Regioni266 – 403Coil 2Add BLAST138
Regioni267 – 387Necessary for interaction with PNNBy similarityAdd BLAST121
Regioni404 – 490TailAdd BLAST87

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi9 – 51Ser-richAdd BLAST43

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IG4R. Eukaryota.
ENOG410YY6B. LUCA.
GeneTreeiENSGT00760000118796.
HOVERGENiHBG013015.
InParanoidiP11679.
KOiK07605.
OMAiXRASLEA.
OrthoDBiEOG091G09KR.
PhylomeDBiP11679.
TreeFamiTF317854.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR032444. Keratin_2_head.
IPR003054. Keratin_II.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 2 hits.
PfamiPF00038. Filament. 1 hit.
PF16208. Keratin_2_head. 1 hit.
[Graphical view]
PRINTSiPR01276. TYPE2KERATIN.
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11679-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIRVTQKSY KMSTSGPRAF SSRSFTSGPG ARISSSSFSR VGSSSSSFRG
60 70 80 90 100
SMGTGVGLGG FGGAGVGGIT AVTVNQSLLS PLKLEVDPNI QAVRTQEKEQ
110 120 130 140 150
IKSLNNKFAS FIDKVRFLEQ QNKMLETKWS LLQQQKTSRS NMDNMFESYI
160 170 180 190 200
NNLRRQLEAL GQEKLKLEAE LGNMQGLVED FKNKYEDEIN KRTEMENEFV
210 220 230 240 250
LIKKDVDEAY MNKVELESRL EGLTDEINFL RQIHEEEIRE LQSQISDTSV
260 270 280 290 300
VLSMDNSRSL DMDGIIAEVR AQYEDIANRS RAEAETMYQI KYEELQTLAG
310 320 330 340 350
KHGDDLRRTK TEISEMNRNI NRLQAEIEAL KGQRASLEAA IADAEQRGEM
360 370 380 390 400
AIKDAQTKLA ELEAALQRAK QDMARQLREY QELMNVKLAL DIEITTYRKL
410 420 430 440 450
LEGEESRLES GMQNMSIHTK TTSGYSGGLS SSYGGLTSPG FSYGMSSFQP
460 470 480 490
GFGSAGGSNT FSRTTKAVVV KKIETRDGKL VSESSDVVSK
Length:490
Mass (Da):54,565
Last modified:January 23, 2007 - v4
Checksum:iC0C79590F0C28502
GO

Sequence cautioni

The sequence AAA37551 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12 – 35MSTSG…ARISS → NVYSVGLLASARSGSWTGSA STA in AAA37548 (PubMed:2467842).CuratedAdd BLAST24
Sequence conflicti40 – 58RVGSS…TGVGL → GRACQLGSSFGEAWHGVS in AAA37548 (PubMed:2467842).CuratedAdd BLAST19
Sequence conflicti52M → L in AAI06155 (PubMed:15489334).Curated1
Sequence conflicti75N → D in AAA37548 (PubMed:2467842).Curated1
Sequence conflicti83 – 87KLEVD → QLSL in AAA37548 (PubMed:2467842).Curated5
Sequence conflicti108F → L in BAE39554 (PubMed:16141072).Curated1
Sequence conflicti190 – 191NK → QQ in CAA31278 (PubMed:2454862).Curated2
Sequence conflicti312E → G in BAE40567 (PubMed:16141072).Curated1
Sequence conflicti325Missing in CAA31278 (PubMed:2454862).Curated1
Sequence conflicti368R → P in CAA31278 (PubMed:2454862).Curated1
Sequence conflicti385N → K in BAE38980 (PubMed:16141072).Curated1
Sequence conflicti385N → K in BAE39006 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12789 mRNA. Translation: CAA31278.1.
X15662 Genomic DNA. Translation: CAA33697.1.
M21836 mRNA. Translation: AAA37550.1.
M21836 mRNA. Translation: AAA37551.1. Different initiation.
M22831 mRNA. Translation: AAA37548.1.
D90360 Genomic DNA. Translation: BAA14375.1.
AK077597 mRNA. Translation: BAC36888.1.
AK145546 mRNA. Translation: BAE26499.1.
AK145679 mRNA. Translation: BAE26585.1.
AK145777 mRNA. Translation: BAE26646.1.
AK146948 mRNA. Translation: BAE27557.1.
AK147044 mRNA. Translation: BAE27630.1.
AK166737 mRNA. Translation: BAE38980.1.
AK166768 mRNA. Translation: BAE39006.1.
AK166854 mRNA. Translation: BAE39072.1.
AK166960 mRNA. Translation: BAE39143.1.
AK166993 mRNA. Translation: BAE39172.1.
AK167120 mRNA. Translation: BAE39268.1.
AK167269 mRNA. Translation: BAE39382.1.
AK167471 mRNA. Translation: BAE39554.1.
AK168522 mRNA. Translation: BAE40401.1.
AK168540 mRNA. Translation: BAE40418.1.
AK168561 mRNA. Translation: BAE40434.1.
AK168726 mRNA. Translation: BAE40567.1.
AK168910 mRNA. Translation: BAE40723.1.
AK169136 mRNA. Translation: BAE40915.1.
AK169691 mRNA. Translation: BAE41308.1.
BC094009 mRNA. Translation: AAH94009.1.
BC106154 mRNA. Translation: AAI06155.1.
CCDSiCCDS27868.1.
PIRiJS0658.
JT0407.
S05474.
RefSeqiNP_112447.2. NM_031170.2.
UniGeneiMm.358618.

Genome annotation databases

EnsembliENSMUST00000023952; ENSMUSP00000023952; ENSMUSG00000049382.
GeneIDi16691.
KEGGimmu:16691.
UCSCiuc007xui.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12789 mRNA. Translation: CAA31278.1.
X15662 Genomic DNA. Translation: CAA33697.1.
M21836 mRNA. Translation: AAA37550.1.
M21836 mRNA. Translation: AAA37551.1. Different initiation.
M22831 mRNA. Translation: AAA37548.1.
D90360 Genomic DNA. Translation: BAA14375.1.
AK077597 mRNA. Translation: BAC36888.1.
AK145546 mRNA. Translation: BAE26499.1.
AK145679 mRNA. Translation: BAE26585.1.
AK145777 mRNA. Translation: BAE26646.1.
AK146948 mRNA. Translation: BAE27557.1.
AK147044 mRNA. Translation: BAE27630.1.
AK166737 mRNA. Translation: BAE38980.1.
AK166768 mRNA. Translation: BAE39006.1.
AK166854 mRNA. Translation: BAE39072.1.
AK166960 mRNA. Translation: BAE39143.1.
AK166993 mRNA. Translation: BAE39172.1.
AK167120 mRNA. Translation: BAE39268.1.
AK167269 mRNA. Translation: BAE39382.1.
AK167471 mRNA. Translation: BAE39554.1.
AK168522 mRNA. Translation: BAE40401.1.
AK168540 mRNA. Translation: BAE40418.1.
AK168561 mRNA. Translation: BAE40434.1.
AK168726 mRNA. Translation: BAE40567.1.
AK168910 mRNA. Translation: BAE40723.1.
AK169136 mRNA. Translation: BAE40915.1.
AK169691 mRNA. Translation: BAE41308.1.
BC094009 mRNA. Translation: AAH94009.1.
BC106154 mRNA. Translation: AAI06155.1.
CCDSiCCDS27868.1.
PIRiJS0658.
JT0407.
S05474.
RefSeqiNP_112447.2. NM_031170.2.
UniGeneiMm.358618.

3D structure databases

ProteinModelPortaliP11679.
SMRiP11679.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201037. 7 interactors.
IntActiP11679. 3 interactors.
MINTiMINT-1859050.
STRINGi10090.ENSMUSP00000023952.

PTM databases

iPTMnetiP11679.
PhosphoSitePlusiP11679.

2D gel databases

SWISS-2DPAGEP11679.

Proteomic databases

MaxQBiP11679.
PaxDbiP11679.
PeptideAtlasiP11679.
PRIDEiP11679.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023952; ENSMUSP00000023952; ENSMUSG00000049382.
GeneIDi16691.
KEGGimmu:16691.
UCSCiuc007xui.1. mouse.

Organism-specific databases

CTDi3856.
MGIiMGI:96705. Krt8.

Phylogenomic databases

eggNOGiENOG410IG4R. Eukaryota.
ENOG410YY6B. LUCA.
GeneTreeiENSGT00760000118796.
HOVERGENiHBG013015.
InParanoidiP11679.
KOiK07605.
OMAiXRASLEA.
OrthoDBiEOG091G09KR.
PhylomeDBiP11679.
TreeFamiTF317854.

Enzyme and pathway databases

ReactomeiR-MMU-6805567. Keratinization.
R-MMU-6809371. Formation of the cornified envelope.

Miscellaneous databases

PROiP11679.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000049382.
CleanExiMM_KRT8.
GenevisibleiP11679. MM.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR032444. Keratin_2_head.
IPR003054. Keratin_II.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 2 hits.
PfamiPF00038. Filament. 1 hit.
PF16208. Keratin_2_head. 1 hit.
[Graphical view]
PRINTSiPR01276. TYPE2KERATIN.
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiK2C8_MOUSE
AccessioniPrimary (citable) accession number: P11679
Secondary accession number(s): Q3KQK5
, Q3TGI1, Q3TJE1, Q3TKY7, Q61463, Q61518, Q61519
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 160 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.