Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Keratin, type II cytoskeletal 8

Gene

Krt8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei347 – 3471Stutter

GO - Molecular functioni

GO - Biological processi

  • cell differentiation involved in embryonic placenta development Source: MGI
  • cell morphogenesis involved in differentiation Source: UniProtKB
  • extrinsic apoptotic signaling pathway Source: MGI
  • hepatocyte apoptotic process Source: MGI
  • multicellular organism development Source: UniProtKB
  • response to hydrostatic pressure Source: Ensembl
  • response to other organism Source: MGI
  • sarcomere organization Source: Ensembl
  • tumor necrosis factor-mediated signaling pathway Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type II cytoskeletal 8
Alternative name(s):
Cytokeratin endo A
Cytokeratin-8
Short name:
CK-8
Keratin-8
Short name:
K8
Type-II keratin Kb8
Gene namesi
Name:Krt8
Synonyms:Krt2-8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:96705. Krt8.

Subcellular locationi

GO - Cellular componenti

  • cell-cell junction Source: MGI
  • cell periphery Source: MGI
  • costamere Source: Ensembl
  • cytoplasm Source: MGI
  • dystrophin-associated glycoprotein complex Source: Ensembl
  • extracellular exosome Source: MGI
  • intermediate filament Source: MGI
  • keratin filament Source: Ensembl
  • nuclear matrix Source: UniProtKB-SubCell
  • nucleoplasm Source: UniProtKB-SubCell
  • nucleus Source: MGI
  • sarcolemma Source: MGI
  • Z disc Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Intermediate filament, Keratin, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Keratin, type II cytoskeletal 8PRO_0000063741Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei9 – 91Phosphoserine; by PKC/PRKCEBy similarity
Modified residuei13 – 131PhosphoserineBy similarity
Modified residuei15 – 151PhosphoserineBy similarity
Modified residuei21 – 211PhosphoserineCombined sources
Modified residuei22 – 221PhosphoserineBy similarity
Modified residuei24 – 241Phosphoserine; by PKC/PRKCECurated
Modified residuei26 – 261PhosphothreonineBy similarity
Modified residuei27 – 271PhosphoserineBy similarity
Modified residuei34 – 341PhosphoserineBy similarity
Modified residuei37 – 371PhosphoserineBy similarity
Modified residuei39 – 391PhosphoserineBy similarity
Modified residuei43 – 431PhosphoserineBy similarity
Modified residuei44 – 441PhosphoserineBy similarity
Modified residuei47 – 471PhosphoserineBy similarity
Modified residuei51 – 511PhosphoserineBy similarity
Modified residuei80 – 801Phosphoserine; by MAPK1 Publication
Modified residuei107 – 1071N6-malonyllysineBy similarity
Cross-linki203 – 203Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki203 – 203Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei213 – 2131N6-acetyllysineBy similarity
Modified residuei259 – 2591PhosphoserineCombined sources
Modified residuei280 – 2801PhosphoserineBy similarity
Cross-linki291 – 291Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei301 – 3011N6-acetyllysineBy similarity
Cross-linki310 – 310Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei331 – 3311N6-acetyllysineBy similarity
Modified residuei336 – 3361PhosphoserineBy similarity
Modified residuei406 – 4061PhosphoserineBy similarity
Modified residuei410 – 4101PhosphoserineBy similarity
Modified residuei416 – 4161PhosphoserineBy similarity
Modified residuei423 – 4231PhosphoserineBy similarity
Modified residuei430 – 4301PhosphoserineBy similarity
Modified residuei432 – 4321PhosphoserineBy similarity
Modified residuei438 – 4381PhosphoserineBy similarity
Cross-linki479 – 479Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki479 – 479Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei482 – 4821PhosphoserineCombined sources
Modified residuei484 – 4841PhosphoserineBy similarity
Modified residuei485 – 4851PhosphoserineCombined sources
Modified residuei489 – 4891PhosphoserineCombined sources

Post-translational modificationi

Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-80 phosphorylation plays an important role in keratin filament reorganization (By similarity).By similarity
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation (By similarity).By similarity
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP11679.
PaxDbiP11679.
PeptideAtlasiP11679.
PRIDEiP11679.

2D gel databases

SWISS-2DPAGEP11679.

PTM databases

iPTMnetiP11679.
PhosphoSiteiP11679.

Expressioni

Tissue specificityi

Expressed in abundance in the epithelia of colon, bladder, ileum, and stomach, with lower expression observed in earskin (at protein level). Also expressed in pancreas, liver, dudenum and jejunum.1 Publication

Gene expression databases

BgeeiENSMUSG00000049382.
CleanExiMM_KRT8.
GenevisibleiP11679. MM.

Interactioni

Subunit structurei

Heterotetramer of two type I and two type II keratins. KRT8 associates with KRT18. Associates with KRT20. Interacts with PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP. Interacts with APEX1 (By similarity). Interacts with GPER1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201037. 7 interactions.
IntActiP11679. 3 interactions.
MINTiMINT-1859050.
STRINGi10090.ENSMUSP00000023952.

Structurei

3D structure databases

ProteinModelPortaliP11679.
SMRiP11679. Positions 94-245, 261-403.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9696HeadAdd
BLAST
Regioni97 – 403307RodAdd
BLAST
Regioni97 – 13236Coil 1AAdd
BLAST
Regioni133 – 14917Linker 1Add
BLAST
Regioni150 – 24192Coil 1BAdd
BLAST
Regioni242 – 26524Linker 12Add
BLAST
Regioni266 – 403138Coil 2Add
BLAST
Regioni267 – 387121Necessary for interaction with PNNBy similarityAdd
BLAST
Regioni404 – 49087TailAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi9 – 5143Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IG4R. Eukaryota.
ENOG410YY6B. LUCA.
GeneTreeiENSGT00760000118796.
HOVERGENiHBG013015.
InParanoidiP11679.
KOiK07605.
OMAiXRASLEA.
OrthoDBiEOG091G09KR.
PhylomeDBiP11679.
TreeFamiTF317854.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR032444. Keratin_2_head.
IPR003054. Keratin_II.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 2 hits.
PfamiPF00038. Filament. 1 hit.
PF16208. Keratin_2_head. 1 hit.
[Graphical view]
PRINTSiPR01276. TYPE2KERATIN.
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11679-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIRVTQKSY KMSTSGPRAF SSRSFTSGPG ARISSSSFSR VGSSSSSFRG
60 70 80 90 100
SMGTGVGLGG FGGAGVGGIT AVTVNQSLLS PLKLEVDPNI QAVRTQEKEQ
110 120 130 140 150
IKSLNNKFAS FIDKVRFLEQ QNKMLETKWS LLQQQKTSRS NMDNMFESYI
160 170 180 190 200
NNLRRQLEAL GQEKLKLEAE LGNMQGLVED FKNKYEDEIN KRTEMENEFV
210 220 230 240 250
LIKKDVDEAY MNKVELESRL EGLTDEINFL RQIHEEEIRE LQSQISDTSV
260 270 280 290 300
VLSMDNSRSL DMDGIIAEVR AQYEDIANRS RAEAETMYQI KYEELQTLAG
310 320 330 340 350
KHGDDLRRTK TEISEMNRNI NRLQAEIEAL KGQRASLEAA IADAEQRGEM
360 370 380 390 400
AIKDAQTKLA ELEAALQRAK QDMARQLREY QELMNVKLAL DIEITTYRKL
410 420 430 440 450
LEGEESRLES GMQNMSIHTK TTSGYSGGLS SSYGGLTSPG FSYGMSSFQP
460 470 480 490
GFGSAGGSNT FSRTTKAVVV KKIETRDGKL VSESSDVVSK
Length:490
Mass (Da):54,565
Last modified:January 23, 2007 - v4
Checksum:iC0C79590F0C28502
GO

Sequence cautioni

The sequence AAA37551 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 3524MSTSG…ARISS → NVYSVGLLASARSGSWTGSA STA in AAA37548 (PubMed:2467842).CuratedAdd
BLAST
Sequence conflicti40 – 5819RVGSS…TGVGL → GRACQLGSSFGEAWHGVS in AAA37548 (PubMed:2467842).CuratedAdd
BLAST
Sequence conflicti52 – 521M → L in AAI06155 (PubMed:15489334).Curated
Sequence conflicti75 – 751N → D in AAA37548 (PubMed:2467842).Curated
Sequence conflicti83 – 875KLEVD → QLSL in AAA37548 (PubMed:2467842).Curated
Sequence conflicti108 – 1081F → L in BAE39554 (PubMed:16141072).Curated
Sequence conflicti190 – 1912NK → QQ in CAA31278 (PubMed:2454862).Curated
Sequence conflicti312 – 3121E → G in BAE40567 (PubMed:16141072).Curated
Sequence conflicti325 – 3251Missing in CAA31278 (PubMed:2454862).Curated
Sequence conflicti368 – 3681R → P in CAA31278 (PubMed:2454862).Curated
Sequence conflicti385 – 3851N → K in BAE38980 (PubMed:16141072).Curated
Sequence conflicti385 – 3851N → K in BAE39006 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12789 mRNA. Translation: CAA31278.1.
X15662 Genomic DNA. Translation: CAA33697.1.
M21836 mRNA. Translation: AAA37550.1.
M21836 mRNA. Translation: AAA37551.1. Different initiation.
M22831 mRNA. Translation: AAA37548.1.
D90360 Genomic DNA. Translation: BAA14375.1.
AK077597 mRNA. Translation: BAC36888.1.
AK145546 mRNA. Translation: BAE26499.1.
AK145679 mRNA. Translation: BAE26585.1.
AK145777 mRNA. Translation: BAE26646.1.
AK146948 mRNA. Translation: BAE27557.1.
AK147044 mRNA. Translation: BAE27630.1.
AK166737 mRNA. Translation: BAE38980.1.
AK166768 mRNA. Translation: BAE39006.1.
AK166854 mRNA. Translation: BAE39072.1.
AK166960 mRNA. Translation: BAE39143.1.
AK166993 mRNA. Translation: BAE39172.1.
AK167120 mRNA. Translation: BAE39268.1.
AK167269 mRNA. Translation: BAE39382.1.
AK167471 mRNA. Translation: BAE39554.1.
AK168522 mRNA. Translation: BAE40401.1.
AK168540 mRNA. Translation: BAE40418.1.
AK168561 mRNA. Translation: BAE40434.1.
AK168726 mRNA. Translation: BAE40567.1.
AK168910 mRNA. Translation: BAE40723.1.
AK169136 mRNA. Translation: BAE40915.1.
AK169691 mRNA. Translation: BAE41308.1.
BC094009 mRNA. Translation: AAH94009.1.
BC106154 mRNA. Translation: AAI06155.1.
CCDSiCCDS27868.1.
PIRiJS0658.
JT0407.
S05474.
RefSeqiNP_112447.2. NM_031170.2.
UniGeneiMm.358618.

Genome annotation databases

EnsembliENSMUST00000023952; ENSMUSP00000023952; ENSMUSG00000049382.
GeneIDi16691.
KEGGimmu:16691.
UCSCiuc007xui.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12789 mRNA. Translation: CAA31278.1.
X15662 Genomic DNA. Translation: CAA33697.1.
M21836 mRNA. Translation: AAA37550.1.
M21836 mRNA. Translation: AAA37551.1. Different initiation.
M22831 mRNA. Translation: AAA37548.1.
D90360 Genomic DNA. Translation: BAA14375.1.
AK077597 mRNA. Translation: BAC36888.1.
AK145546 mRNA. Translation: BAE26499.1.
AK145679 mRNA. Translation: BAE26585.1.
AK145777 mRNA. Translation: BAE26646.1.
AK146948 mRNA. Translation: BAE27557.1.
AK147044 mRNA. Translation: BAE27630.1.
AK166737 mRNA. Translation: BAE38980.1.
AK166768 mRNA. Translation: BAE39006.1.
AK166854 mRNA. Translation: BAE39072.1.
AK166960 mRNA. Translation: BAE39143.1.
AK166993 mRNA. Translation: BAE39172.1.
AK167120 mRNA. Translation: BAE39268.1.
AK167269 mRNA. Translation: BAE39382.1.
AK167471 mRNA. Translation: BAE39554.1.
AK168522 mRNA. Translation: BAE40401.1.
AK168540 mRNA. Translation: BAE40418.1.
AK168561 mRNA. Translation: BAE40434.1.
AK168726 mRNA. Translation: BAE40567.1.
AK168910 mRNA. Translation: BAE40723.1.
AK169136 mRNA. Translation: BAE40915.1.
AK169691 mRNA. Translation: BAE41308.1.
BC094009 mRNA. Translation: AAH94009.1.
BC106154 mRNA. Translation: AAI06155.1.
CCDSiCCDS27868.1.
PIRiJS0658.
JT0407.
S05474.
RefSeqiNP_112447.2. NM_031170.2.
UniGeneiMm.358618.

3D structure databases

ProteinModelPortaliP11679.
SMRiP11679. Positions 94-245, 261-403.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201037. 7 interactions.
IntActiP11679. 3 interactions.
MINTiMINT-1859050.
STRINGi10090.ENSMUSP00000023952.

PTM databases

iPTMnetiP11679.
PhosphoSiteiP11679.

2D gel databases

SWISS-2DPAGEP11679.

Proteomic databases

MaxQBiP11679.
PaxDbiP11679.
PeptideAtlasiP11679.
PRIDEiP11679.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023952; ENSMUSP00000023952; ENSMUSG00000049382.
GeneIDi16691.
KEGGimmu:16691.
UCSCiuc007xui.1. mouse.

Organism-specific databases

CTDi3856.
MGIiMGI:96705. Krt8.

Phylogenomic databases

eggNOGiENOG410IG4R. Eukaryota.
ENOG410YY6B. LUCA.
GeneTreeiENSGT00760000118796.
HOVERGENiHBG013015.
InParanoidiP11679.
KOiK07605.
OMAiXRASLEA.
OrthoDBiEOG091G09KR.
PhylomeDBiP11679.
TreeFamiTF317854.

Miscellaneous databases

PROiP11679.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000049382.
CleanExiMM_KRT8.
GenevisibleiP11679. MM.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR032444. Keratin_2_head.
IPR003054. Keratin_II.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 2 hits.
PfamiPF00038. Filament. 1 hit.
PF16208. Keratin_2_head. 1 hit.
[Graphical view]
PRINTSiPR01276. TYPE2KERATIN.
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiK2C8_MOUSE
AccessioniPrimary (citable) accession number: P11679
Secondary accession number(s): Q3KQK5
, Q3TGI1, Q3TJE1, Q3TKY7, Q61463, Q61518, Q61519
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 157 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.