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P11678

- PERE_HUMAN

UniProt

P11678 - PERE_HUMAN

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Protein

Eosinophil peroxidase

Gene
EPX, EPER, EPO, EPP
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis.2 Publications

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Binds 1 calcium ion per heterodimer By similarity.
Binds 1 heme B (iron-protoporphyrin IX) covalently through ester linkages to hydroxylated methyl groups formed auto-catalytically with hydrogen peroxide at the heme C-1 and C-5 positions. The ester linkage to Asp-232 was observed in 30% of the chains.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei232 – 2321Heme (covalent; via 2 links); partial
Active sitei233 – 2331Proton acceptor By similarity
Metal bindingi234 – 2341Calcium By similarity
Metal bindingi306 – 3061Calcium By similarity
Metal bindingi308 – 3081Calcium; via carbonyl oxygen By similarity
Metal bindingi310 – 3101Calcium By similarity
Metal bindingi312 – 3121Calcium By similarity
Sitei377 – 3771Transition state stabilizer By similarity
Binding sitei380 – 3801Heme (covalent; via 2 links)
Metal bindingi474 – 4741Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. metal ion binding Source: UniProtKB-KW
  3. peroxidase activity Source: ProtInc

GO - Biological processi

  1. defense response to nematode Source: Ensembl
  2. eosinophil migration Source: Ensembl
  3. hydrogen peroxide catabolic process Source: UniProtKB-KW
  4. negative regulation of interleukin-10 production Source: Ensembl
  5. negative regulation of interleukin-5 production Source: Ensembl
  6. positive regulation of interleukin-4 production Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Protein family/group databases

PeroxiBasei3317. HsEPO.

Names & Taxonomyi

Protein namesi
Recommended name:
Eosinophil peroxidase (EC:1.11.1.7)
Short name:
EPO
Cleaved into the following 2 chains:
Gene namesi
Name:EPX
Synonyms:EPER, EPO, EPP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:3423. EPX.

Subcellular locationi

Cytoplasmic granule
Note: Cytoplasmic granules of eosinophils.

Pathology & Biotechi

Involvement in diseasei

Eosinophil peroxidase deficiency (EPXD) [MIM:261500]: A rare abnormality without clinical symptoms characterized by decreased or absent peroxidase activity and decreased volume of the granule matrix in eosinophils.
Note: The disease is caused by mutations affecting the gene represented in this entry.1 Publication
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti286 – 2861R → H in EPXD. 1 Publication
Corresponds to variant rs121434566 [ dbSNP | Ensembl ].
VAR_015376

Keywords - Diseasei

Disease mutation

Organism-specific databases

MIMi261500. phenotype.
PharmGKBiPA27841.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717 Reviewed predictionAdd
BLAST
Propeptidei18 – 139122PRO_0000023639Add
BLAST
Chaini140 – 250111Eosinophil peroxidase light chainPRO_0000023640Add
BLAST
Chaini251 – 715465Eosinophil peroxidase heavy chainPRO_0000023641Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi52 – 521N-linked (GlcNAc...) Reviewed prediction
Glycosylationi113 – 1131N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi141 ↔ 152 By similarity
Disulfide bondi253 ↔ 263 By similarity
Disulfide bondi257 ↔ 281 By similarity
Glycosylationi327 – 3271N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi359 ↔ 370 By similarity
Glycosylationi363 – 3631N-linked (GlcNAc...) Reviewed prediction
Modified residuei488 – 4881Nitrated tyrosine1 Publication
Disulfide bondi578 ↔ 635 By similarity
Disulfide bondi676 ↔ 701 By similarity
Glycosylationi700 – 7001N-linked (GlcNAc...) Reviewed prediction
Glycosylationi708 – 7081N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein, Nitration

Proteomic databases

PaxDbiP11678.
PRIDEiP11678.

PTM databases

PhosphoSiteiP11678.

Expressioni

Gene expression databases

BgeeiP11678.
CleanExiHS_EPO.
HS_EPX.
GenevestigatoriP11678.

Organism-specific databases

HPAiHPA050507.

Interactioni

Subunit structurei

Tetramer of two light chains and two heavy chains.

Protein-protein interaction databases

IntActiP11678. 3 interactions.
STRINGi9606.ENSP00000225371.

Structurei

3D structure databases

ProteinModelPortaliP11678.
SMRiP11678. Positions 143-713.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG262194.
HOGENOMiHOG000016084.
HOVERGENiHBG000071.
InParanoidiP11678.
KOiK10788.
OMAiMHVALGL.
OrthoDBiEOG7M0NQW.
PhylomeDBiP11678.
TreeFamiTF314316.

Family and domain databases

Gene3Di1.10.640.10. 1 hit.
InterProiIPR029599. EPX/EPO.
IPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
[Graphical view]
PANTHERiPTHR11475:SF49. PTHR11475:SF49. 1 hit.
PfamiPF03098. An_peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00457. ANPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS50292. PEROXIDASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11678-1 [UniParc]FASTAAdd to Basket

« Hide

MHLLPALAGV LATLVLAQPC EGTDPASPGA VETSVLRDCI AEAKLLVDAA    50
YNWTQKSIKQ RLRSGSASPM DLLSYFKQPV AATRTVVRAA DYMHVALGLL 100
EEKLQPQRSG PFNVTDVLTE PQLRLLSQAS GCALRDQAER CSDKYRTITG 150
RCNNKRRPLL GASNQALARW LPAEYEDGLS LPFGWTPSRR RNGFLLPLVR 200
AVSNQIVRFP NERLTSDRGR ALMFMQWGQF IDHDLDFSPE SPARVAFTAG 250
VDCERTCAQL PPCFPIKIPP NDPRIKNQRD CIPFFRSAPS CPQNKNRVRN 300
QINALTSFVD ASMVYGSEVS LSLRLRNRTN YLGLLAINQR FQDNGRALLP 350
FDNLHDDPCL LTNRSARIPC FLAGDTRSTE TPKLAAMHTL FMREHNRLAT 400
ELRRLNPRWN GDKLYNEARK IMGAMVQIIT YRDFLPLVLG KARARRTLGH 450
YRGYCSNVDP RVANVFTLAF RFGHTMLQPF MFRLDSQYRA SAPNSHVPLS 500
SAFFASWRIV YEGGIDPILR GLMATPAKLN RQDAMLVDEL RDRLFRQVRR 550
IGLDLAALNM QRSRDHGLPG YNAWRRFCGL SQPRNLAQLS RVLKNQDLAR 600
KFLNLYGTPD NIDIWIGAIA EPLLPGARVG PLLACLFENQ FRRARDGDRF 650
WWQKRGVFTK RQRKALSRIS LSRIICDNTG ITTVSRDIFR ANIYPRGFVN 700
CSRIPRLNLS AWRGT 715
Length:715
Mass (Da):81,040
Last modified:February 1, 1996 - v2
Checksum:iCEB4E689A6C46374
GO

Polymorphismi

Allelic variant in EPX is associated with Japanese cedar pollinosis which is a type I allergic disease with ocular and nasal symptoms that develop paroxysmally on contact with Japanese cedar pollen. These symptoms, which occur seasonally each year, are typical features of allergic rhinitis, such as sneezing, excessive nasal secretion, nasal congestion, and conjunctival itching.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti35 – 351V → I.
Corresponds to variant rs34553736 [ dbSNP | Ensembl ].
VAR_050485
Natural varianti40 – 401I → M.1 Publication
Corresponds to variant rs11079339 [ dbSNP | Ensembl ].
VAR_025138
Natural varianti122 – 1221Q → H.1 Publication
Corresponds to variant rs11652709 [ dbSNP | Ensembl ].
VAR_025139
Natural varianti249 – 2491A → E.1 Publication
Corresponds to variant rs35896669 [ dbSNP | Ensembl ].
VAR_025140
Natural varianti276 – 2761K → R.1 Publication
Corresponds to variant rs35074452 [ dbSNP | Ensembl ].
VAR_025141
Natural varianti286 – 2861R → H in EPXD. 1 Publication
Corresponds to variant rs121434566 [ dbSNP | Ensembl ].
VAR_015376
Natural varianti292 – 2921P → L.1 Publication
Corresponds to variant rs33971258 [ dbSNP | Ensembl ].
VAR_025142
Natural varianti326 – 3261R → H.1 Publication
Corresponds to variant rs35832094 [ dbSNP | Ensembl ].
VAR_060197
Natural varianti326 – 3261R → L.1 Publication
VAR_060198
Natural varianti326 – 3261R → P.1 Publication
Corresponds to variant rs35832094 [ dbSNP | Ensembl ].
VAR_025143
Natural varianti358 – 3581P → L Associated with Japanese cedar pollinosis. 2 Publications
Corresponds to variant rs35135976 [ dbSNP | Ensembl ].
VAR_025144
Natural varianti364 – 3641R → H.1 Publication
Corresponds to variant rs35232062 [ dbSNP | Ensembl ].
VAR_025145
Natural varianti441 – 4411K → T.1 Publication
Corresponds to variant rs35750729 [ dbSNP | Ensembl ].
VAR_025146
Natural varianti458 – 4581V → M.
Corresponds to variant rs34817773 [ dbSNP | Ensembl ].
VAR_050486
Natural varianti496 – 4961H → Q.1 Publication
Corresponds to variant rs33955150 [ dbSNP | Ensembl ].
VAR_025147
Natural varianti572 – 5721N → Y.1 Publication
Corresponds to variant rs2302311 [ dbSNP | Ensembl ].
VAR_020031

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 186TLVLAQ → EFRGQD AA sequence 1 Publication
Sequence conflicti21 – 211E → Q AA sequence 1 Publication
Sequence conflicti113 – 1131N → I in CAA32530. 1 Publication
Sequence conflicti163 – 1631S → C AA sequence 1 Publication
Sequence conflicti645 – 66016RDGDR…GVFTK → ETETGSGGRTRCFHQ AA sequence 1 PublicationAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M29913
, M29904, M29905, M29906, M29907, M29908, M29909, M29910, M29911, M29912 Genomic DNA. Translation: AAA58458.1.
DQ054598 Genomic DNA. Translation: AAY43126.1.
X14346 mRNA. Translation: CAA32530.1.
CCDSiCCDS11602.1.
PIRiA34408.
RefSeqiNP_000493.1. NM_000502.4.
XP_006722191.1. XM_006722128.1.
UniGeneiHs.279259.

Genome annotation databases

EnsembliENST00000225371; ENSP00000225371; ENSG00000121053.
GeneIDi8288.
KEGGihsa:8288.
UCSCiuc002ivq.3. human.

Polymorphism databases

DMDMi1352738.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M29913
, M29904 , M29905 , M29906 , M29907 , M29908 , M29909 , M29910 , M29911 , M29912 Genomic DNA. Translation: AAA58458.1 .
DQ054598 Genomic DNA. Translation: AAY43126.1 .
X14346 mRNA. Translation: CAA32530.1 .
CCDSi CCDS11602.1.
PIRi A34408.
RefSeqi NP_000493.1. NM_000502.4.
XP_006722191.1. XM_006722128.1.
UniGenei Hs.279259.

3D structure databases

ProteinModelPortali P11678.
SMRi P11678. Positions 143-713.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P11678. 3 interactions.
STRINGi 9606.ENSP00000225371.

Chemistry

ChEMBLi CHEMBL2438.

Protein family/group databases

PeroxiBasei 3317. HsEPO.

PTM databases

PhosphoSitei P11678.

Polymorphism databases

DMDMi 1352738.

Proteomic databases

PaxDbi P11678.
PRIDEi P11678.

Protocols and materials databases

DNASUi 8288.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000225371 ; ENSP00000225371 ; ENSG00000121053 .
GeneIDi 8288.
KEGGi hsa:8288.
UCSCi uc002ivq.3. human.

Organism-specific databases

CTDi 8288.
GeneCardsi GC17P056270.
H-InvDB HIX0202542.
HGNCi HGNC:3423. EPX.
HPAi HPA050507.
MIMi 131399. gene.
261500. phenotype.
neXtProti NX_P11678.
PharmGKBi PA27841.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG262194.
HOGENOMi HOG000016084.
HOVERGENi HBG000071.
InParanoidi P11678.
KOi K10788.
OMAi MHVALGL.
OrthoDBi EOG7M0NQW.
PhylomeDBi P11678.
TreeFami TF314316.

Miscellaneous databases

GeneWikii Eosinophil_peroxidase.
GenomeRNAii 8288.
NextBioi 31057.
PROi P11678.
SOURCEi Search...

Gene expression databases

Bgeei P11678.
CleanExi HS_EPO.
HS_EPX.
Genevestigatori P11678.

Family and domain databases

Gene3Di 1.10.640.10. 1 hit.
InterProi IPR029599. EPX/EPO.
IPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
[Graphical view ]
PANTHERi PTHR11475:SF49. PTHR11475:SF49. 1 hit.
Pfami PF03098. An_peroxidase. 1 hit.
[Graphical view ]
PRINTSi PR00457. ANPEROXIDASE.
SUPFAMi SSF48113. SSF48113. 1 hit.
PROSITEi PS00435. PEROXIDASE_1. 1 hit.
PS50292. PEROXIDASE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and characterization of a chromosomal gene for human eosinophil peroxidase."
    Sakamaki K., Tomonaga M., Tsukui K., Nagata S.
    J. Biol. Chem. 264:16828-16836(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Placenta.
  2. NIEHS SNPs program
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS MET-40; HIS-122; GLU-249; ARG-276; LEU-292; PRO-326; LEU-358; HIS-364; THR-441; GLN-496 AND TYR-572.
  3. "Molecular cloning of the human eosinophil peroxidase. Evidence for the existence of a peroxidase multigene family."
    Ten R.M., Pease L.R., McKean D.J., Bell M.P., Gleich G.J.
    J. Exp. Med. 169:1757-1769(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-715, PROTEIN SEQUENCE OF 140-187 AND 251-288.
    Tissue: Blood.
  4. "Biochemical evidence for heme linkage through esters with Asp-93 and Glu-241 in human eosinophil peroxidase. The ester with Asp-93 is only partially formed in vivo."
    Oxvig C., Thomsen A.R., Overgaard M.T., Sorensen E.S., Hoejrup P., Bjerrum M.J., Gleich G.J., Sottrup-Jensen L.
    J. Biol. Chem. 274:16953-16958(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: COVALENT HEME ATTACHMENT, PARTIAL PROTEIN SEQUENCE.
    Tissue: Blood.
  5. "Human eosinophil peroxidase induces surface alteration, killing, and lysis of Mycobacterium tuberculosis."
    Borelli V., Vita F., Shankar S., Soranzo M.R., Banfi E., Scialino G., Brochetta C., Zabucchi G.
    Infect. Immun. 71:605-613(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: FUNCTION, NITRATION AT TYR-488.
  7. "Hereditary eosinophil peroxidase deficiency: immunochemical and spectroscopic studies and evidence for a compound heterozygosity of the defect."
    Romano M., Patriarca P., Melo C., Baralle F.E., Dri P.
    Proc. Natl. Acad. Sci. U.S.A. 91:12496-12500(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT EPXD HIS-286.
  8. "High contribution contrast between the genes of eosinophil peroxidase and IL-4 receptor alpha-chain in Japanese cedar pollinosis."
    Nakamura H., Miyagawa K., Ogino K., Endo T., Imai T., Ozasa K., Motohashi Y., Matsuzaki I., Sasahara S., Hatta K., Eboshida A.
    J. Allergy Clin. Immunol. 112:1127-1131(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS HIS-326; LEU-326 AND LEU-358, POLYMORPHISM.

Entry informationi

Entry nameiPERE_HUMAN
AccessioniPrimary (citable) accession number: P11678
Secondary accession number(s): Q4TVP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: February 1, 1996
Last modified: September 3, 2014
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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